sodium orthovanadate  (Selleck Chemicals)

 
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    Name:
    Sodium orthovanadate
    Description:
    Sodium orthovanadate is an alkaline phosphatase and Na K ATPase inhibitor with IC50 of 10 μM
    Catalog Number:
    s2000
    Molecular Weight:
    183.91
    Price:
    None
    Category:
    ATPase inhibitor Transmembrane Transporters
    Formula:
    Na3O4V
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    Structured Review

    Selleck Chemicals sodium orthovanadate
    Sodium orthovanadate is an alkaline phosphatase and Na K ATPase inhibitor with IC50 of 10 μM
    https://www.bioz.com/result/sodium orthovanadate/product/Selleck Chemicals
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium orthovanadate - by Bioz Stars, 2020-07
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: .. Western blot analysis T lymphocytes were washed twice with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer [25 mM Tris-HCl pH 7.5 (Shanghai Jiang Lai Biological Technology Co., Ltd.), 150 mM NaCl (Shanghai Jiang Lai Biological Technology Co., Ltd.), 1% Nonidet P-40 (Sangon Biological Engineering Co., Ltd.), 5 mM sodium pyrophosphate (Xinxiang Huaxing Chemical Co., Ltd., Xinxiang, China), 1 mM sodium orthovanadate (Selleck Chemicals, Houston, TX, USA), 10 mM sodium fluoride (Shanghai Kunxin Chemical Technology Co., Ltd., Zibo, China), 10% glycerol (Solarbio Science & Technology, Beijing, China), 1 mM phenylmethylsulfonyl fluoride (Chemical Reagent Co., Ltd., Shanghai, China), 25 µ g/ml leupeptin (Shanghai Hufeng Chemical Co., Ltd., Shanghai, China), 25 µ g/ml aprotinin (Shanghai Hufeng Chemical Co., Ltd.) and 2 µ g/ml pepstatin (Shanghai Hufeng Chemical Co., Ltd.)] and incubated for 30 min on ice. .. The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.

    Incubation:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: .. Western blot analysis T lymphocytes were washed twice with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer [25 mM Tris-HCl pH 7.5 (Shanghai Jiang Lai Biological Technology Co., Ltd.), 150 mM NaCl (Shanghai Jiang Lai Biological Technology Co., Ltd.), 1% Nonidet P-40 (Sangon Biological Engineering Co., Ltd.), 5 mM sodium pyrophosphate (Xinxiang Huaxing Chemical Co., Ltd., Xinxiang, China), 1 mM sodium orthovanadate (Selleck Chemicals, Houston, TX, USA), 10 mM sodium fluoride (Shanghai Kunxin Chemical Technology Co., Ltd., Zibo, China), 10% glycerol (Solarbio Science & Technology, Beijing, China), 1 mM phenylmethylsulfonyl fluoride (Chemical Reagent Co., Ltd., Shanghai, China), 25 µ g/ml leupeptin (Shanghai Hufeng Chemical Co., Ltd., Shanghai, China), 25 µ g/ml aprotinin (Shanghai Hufeng Chemical Co., Ltd.) and 2 µ g/ml pepstatin (Shanghai Hufeng Chemical Co., Ltd.)] and incubated for 30 min on ice. .. The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.

    Article Title: Differential effects of histone deacetylase inhibitors on cellular drug transporters and their implications for using epigenetic modifiers in combination chemotherapy
    Article Snippet: .. To inhibit efflux of daunorubicin, 2 mM sodium orthovanadate (Selleck Chemicals) was added 1 hr after the addition of daunorubicin and incubated for another 30 min. Efflux assay was performed as described above in the presence of 2 mM sodium orthovanadate. .. Part of this research was performed in the Flow Cytometry & Cellular Imaging Facility, which is supported in part by the National Institutes of Health through M.D.

    Lysis:

    Article Title: Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-α-induced protein 8-like-2 in septic rats
    Article Snippet: .. Western blot analysis T lymphocytes were washed twice with ice-cold phosphate-buffered saline (PBS) and lysed in lysis buffer [25 mM Tris-HCl pH 7.5 (Shanghai Jiang Lai Biological Technology Co., Ltd.), 150 mM NaCl (Shanghai Jiang Lai Biological Technology Co., Ltd.), 1% Nonidet P-40 (Sangon Biological Engineering Co., Ltd.), 5 mM sodium pyrophosphate (Xinxiang Huaxing Chemical Co., Ltd., Xinxiang, China), 1 mM sodium orthovanadate (Selleck Chemicals, Houston, TX, USA), 10 mM sodium fluoride (Shanghai Kunxin Chemical Technology Co., Ltd., Zibo, China), 10% glycerol (Solarbio Science & Technology, Beijing, China), 1 mM phenylmethylsulfonyl fluoride (Chemical Reagent Co., Ltd., Shanghai, China), 25 µ g/ml leupeptin (Shanghai Hufeng Chemical Co., Ltd., Shanghai, China), 25 µ g/ml aprotinin (Shanghai Hufeng Chemical Co., Ltd.) and 2 µ g/ml pepstatin (Shanghai Hufeng Chemical Co., Ltd.)] and incubated for 30 min on ice. .. The proteins were separated using 4–12% gradient SDS-PAGE and transferred onto polyvinylidene difluoride membranes.

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  • 90
    Selleck Chemicals sodium orthovanadate
    Sodium Orthovanadate, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium orthovanadate/product/Selleck Chemicals
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium orthovanadate - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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