sodium dodecyl sulfate sds protease k  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Sodium dodecyl sulfate solution
    Description:

    Catalog Number:
    71736
    Price:
    None
    Applications:
    Sodium dodecyl sulfate solution has been used:. in the preparation of 2x extraction buffer. as a component in elution buffer for immunoprecipitation. in SDS-polyacrylamide gel electrophoresis (PAGE) analysis
    Buy from Supplier


    Structured Review

    Millipore sodium dodecyl sulfate sds protease k
    Sodium dodecyl sulfate solution

    https://www.bioz.com/result/sodium dodecyl sulfate sds protease k/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium dodecyl sulfate sds protease k - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    swine testicular st cells
    ppv strain ljl12

    Images

    Related Articles

    Centrifugation:

    Article Title: Role of protein kinase C ? and vascular endothelial growth factor receptor in malignant pleural mesothelioma: Therapeutic implications and the usefulness of Caenorhabditis elegans model organism
    Article Snippet: The cell lysates were cleared by centrifugation at 14,000 g for 15 minutes. .. Protein lysates (60–80 µ g) were separated by 7.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred to PVDF membranes (Millipore, Bedford, MA, USA).

    Article Title: A host cell membrane protein, golgin-97, is essential for poxvirus morphogenesis
    Article Snippet: The cells were lysed by freeze-thawing and cleared by centrifugation at 1500 × g, 4°C. .. Proteins were separated by sodium dodecyl sulfate (SDS) – polyacrylamide gel electrophoresis (PAGE) and transferred to PVDF-membrane (Immobilon-PSQ , Millipore, Billerica, MA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Activation of mineralocorticoid receptor by ecdysone, an adaptogenic and anabolic ecdysteroid, promotes glomerular injury and proteinuria involving overactive GSK3β pathway signaling
    Article Snippet: The concentrations of albumin in urine samples were measured by using mouse albumin ELISA quantitation kit (Bethyl Laboratories Inc., Montgomery, TX). .. To discern the composition of urine proteins, urine samples were processed for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with Coomassie Brilliant Blue (Sigma-Aldrich, St. Louis, MO).

    Incubation:

    Article Title: A host cell membrane protein, golgin-97, is essential for poxvirus morphogenesis
    Article Snippet: Proteins were separated by sodium dodecyl sulfate (SDS) – polyacrylamide gel electrophoresis (PAGE) and transferred to PVDF-membrane (Immobilon-PSQ , Millipore, Billerica, MA). .. The membrane was blocked in 3% gelatin in TTBS (0.05% Tween 20– 150mM NaCl - 50mM TrisHCl, pH 7.4) and incubated with primary anti-golgin-97 (1 μg/ml) or anti-actin antibodies (1:1000) in 1% gelatin - TTBS overnight.

    Article Title: Adenylyl cyclase type 6 overexpression selectively enhances ?-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts
    Article Snippet: Equal volumes of fractions were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Millipore) by electroblotting. .. Membranes were blocked in PBS–Tween with 5% nonfat dry milk and incubated with primary antibody (see “ ”) overnight at 4°C.

    Article Title: Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii ▿
    Article Snippet: Briefly, C. burnetii NMII cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an Immobilon-P membrane (Millipore, Bedford, MA). .. The membrane was placed in a Miniblotter 25 multichannel screening apparatus (Immunetics, Cambridge, MA) and incubated with undiluted hybridoma culture supernatants.

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature. .. Reactive bands were developed and enhanced by SuperSignal West Pico chemiluminescent detection reagents according to the instructions of the manufacturer (Thermo Scientific, Rockford, IL, USA).

    Expressing:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: Paragraph title: Expression and purification of HPV16 E7-binding affibody molecules ... The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma).

    BIA-KA:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma). .. The purity of purified proteins was verified by SDS-PAGE, and the protein concentrations were determined by the bicinchoninic acid (BCA) protein quantitation method.

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: The protein concentration of the samples was determined using Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA). .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature.

    Modification:

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata: Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: Purification and Determination of the Partial Amino Acid Sequence of the 47 kDa Protein To determine the N-terminal amino acid sequence of this 47 kDa protein, proteins of the urea-soluble fraction dissolved in 30 mM Tris-HCl (pH 8.0) were separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinylidene fluoride membrane (Millipore) using a dry blotting system (Nippon Eido). .. However, as the N-terminal amino acid was modified, this approach failed.

    Western Blot:

    Article Title: Distinct Domains in the Adenovirus E3 RID? Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor
    Article Snippet: Paragraph title: Western blot analysis. ... Membrane pellets were solubilized in 2× Laemmli buffer, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to Immobilon-P membranes (Millipore, Bedford, Mass.).

    Article Title: MEG3 Activated by Vitamin D Inhibits Colorectal Cancer Cells Proliferation and Migration via Regulating Clusterin
    Article Snippet: Paragraph title: Western Blotting ... 20 μg total proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA).

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: .. The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma). .. The recombinant protein was purified by affinity chromatography using precharged Ni-NTA Sepharose column (Qiagen) and refolded when dialyzed in PBS using Slide-A-Lyzer (Pierce) according to the manufacturer's recommendations.

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: Paragraph title: Western Blot Analysis ... Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature.

    Article Title: A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example
    Article Snippet: Paragraph title: Western blot analysis ... Proteins are resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred of proteins from gel to NC membrane (Millipore).

    Transformation Assay:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: Following confirmation of the inserted sequences by enzyme digestion and DNA sequencing, positive plasmids were transformed into E. coli BL21 (DE3) for expression of the fusion proteins. .. The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma).

    Transfection:

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: Western Blot Analysis Cos-1 cells were harvested 48 h after transfection and lysed either in RIPA lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM EDTA, 1 mM Na3VO4, 0.5 ug/ml leupeptin] or in NP40 lysis buffer [50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 1 mM henylmethylsulfonyl fluoride, 2 mM sodium pyrophosphate, 25 mM β-glycerophosphate, 1 mM EDTA, 1 mM Na3VO4, 0.5 ug/ml leupeptin]. .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature.

    Protease Inhibitor:

    Article Title: Role of protein kinase C ? and vascular endothelial growth factor receptor in malignant pleural mesothelioma: Therapeutic implications and the usefulness of Caenorhabditis elegans model organism
    Article Snippet: The cells were washed twice with ice-cold phosphate buffered saline (PBS), and whole cell lysates were prepared in RIPA lysis buffer [50 mM Tris (pH 8.0), 150 mM NaCl, 10% glycerol, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and 0.42% NaF containing protease inhibitor cocktail (1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 5 mg/ml leupeptin)]. .. Protein lysates (60–80 µ g) were separated by 7.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred to PVDF membranes (Millipore, Bedford, MA, USA).

    Article Title: Adenylyl cyclase type 6 overexpression selectively enhances ?-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts
    Article Snippet: Briefly, cells were homogenized in 500 mM sodium carbonate plus mammalian protease inhibitor cocktail (Sigma cat# P-8340) with 20 strokes in a Dounce homogenizer, three 10-s bursts in a tissue grinder, then three 20-s bursts with an ultrasound cell disruptor. .. Equal volumes of fractions were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Millipore) by electroblotting.

    Infection:

    Article Title: Distinct Domains in the Adenovirus E3 RID? Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor
    Article Snippet: Cells were infected with Ad mutants at 100 to 200 PFU per cell. .. Membrane pellets were solubilized in 2× Laemmli buffer, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to Immobilon-P membranes (Millipore, Bedford, Mass.).

    Inhibition:

    Article Title: A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example
    Article Snippet: Western blot analysis To monitor the inhibition of virally encoded protein by compounds, we infect the host cells with virus for 24 or 48 h. Cells are then lysed in a buffer containing 50 mmol/L Tris-HCl, pH 8.0, 150 mmol/L NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mmol/L EDTA, 1 mmol/L NaVO4 , 10 mmol/L NaF, and protease inhibitors, and the protein concentration in the lysates was determined by a spectrophotometer. .. Proteins are resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred of proteins from gel to NC membrane (Millipore).

    DNA Sequencing:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: Following confirmation of the inserted sequences by enzyme digestion and DNA sequencing, positive plasmids were transformed into E. coli BL21 (DE3) for expression of the fusion proteins. .. The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma).

    Protein Concentration:

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: The protein concentration of the samples was determined using Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA). .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature.

    Sequencing:

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata: Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: .. Purification and Determination of the Partial Amino Acid Sequence of the 47 kDa Protein To determine the N-terminal amino acid sequence of this 47 kDa protein, proteins of the urea-soluble fraction dissolved in 30 mM Tris-HCl (pH 8.0) were separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinylidene fluoride membrane (Millipore) using a dry blotting system (Nippon Eido). .. After Ponceau S staining, the band was cut out and then subjected to N-terminal amino acid sequence analysis.

    Sonication:

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: The cell lysates in NP-40 lysis buffer were further sonicated and centrifuged to obtain fractions containing soluble supernatants and insoluble pellets. .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature.

    Recombinant:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: .. The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma). .. The recombinant protein was purified by affinity chromatography using precharged Ni-NTA Sepharose column (Qiagen) and refolded when dialyzed in PBS using Slide-A-Lyzer (Pierce) according to the manufacturer's recommendations.

    Imaging:

    Article Title: MEG3 Activated by Vitamin D Inhibits Colorectal Cancer Cells Proliferation and Migration via Regulating Clusterin
    Article Snippet: 20 μg total proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The bands were observed with the enhanced chemiluminescence western blotting detection kit and visualized with a Tanon luminescent imaging workstation (Tanon, Shanghai, China).

    Nucleic Acid Electrophoresis:

    Article Title: Distinct Domains in the Adenovirus E3 RID? Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor
    Article Snippet: .. Membrane pellets were solubilized in 2× Laemmli buffer, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to Immobilon-P membranes (Millipore, Bedford, Mass.). .. For analysis of Fas degradation, proteins solubilized at 18 h postinfection (p.i.) in radioimmunoprecipitation assay buffer were transferred to Immobilon-P. Rabbit antiserum P77-91, which was raised against a synthetic peptide corresponding to amino acids 77 to 91 of RIDα, was used at a dilution of 1:200.

    Article Title: Adenylyl cyclase type 6 overexpression selectively enhances ?-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts
    Article Snippet: .. Equal volumes of fractions were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Millipore) by electroblotting. .. Membranes were blocked in PBS–Tween with 5% nonfat dry milk and incubated with primary antibody (see “ ”) overnight at 4°C.

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: .. The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma). .. The recombinant protein was purified by affinity chromatography using precharged Ni-NTA Sepharose column (Qiagen) and refolded when dialyzed in PBS using Slide-A-Lyzer (Pierce) according to the manufacturer's recommendations.

    Article Title: Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii ▿
    Article Snippet: .. Briefly, C. burnetii NMII cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an Immobilon-P membrane (Millipore, Bedford, MA). .. The membrane was placed in a Miniblotter 25 multichannel screening apparatus (Immunetics, Cambridge, MA) and incubated with undiluted hybridoma culture supernatants.

    Isolation:

    Article Title: Distinct Domains in the Adenovirus E3 RID? Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor
    Article Snippet: For the analysis of RID in the membrane fraction, membranes were isolated as previously described ( ). .. Membrane pellets were solubilized in 2× Laemmli buffer, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to Immobilon-P membranes (Millipore, Bedford, Mass.).

    Article Title: Regulation of Phosphoinositide 3-Kinase by Its Intrinsic Serine Kinase Activity In Vivo
    Article Snippet: .. Isolated protein or immunoprecipitates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon P; Millipore). .. Blots were probed with the indicated antibodies, followed by a horseradish peroxidase-conjugated secondary antibody.

    Protein Quantitation:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma). .. The purity of purified proteins was verified by SDS-PAGE, and the protein concentrations were determined by the bicinchoninic acid (BCA) protein quantitation method.

    Purification:

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata: Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: .. Purification and Determination of the Partial Amino Acid Sequence of the 47 kDa Protein To determine the N-terminal amino acid sequence of this 47 kDa protein, proteins of the urea-soluble fraction dissolved in 30 mM Tris-HCl (pH 8.0) were separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinylidene fluoride membrane (Millipore) using a dry blotting system (Nippon Eido). .. After Ponceau S staining, the band was cut out and then subjected to N-terminal amino acid sequence analysis.

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: Paragraph title: Expression and purification of HPV16 E7-binding affibody molecules ... The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma).

    Article Title: Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii ▿
    Article Snippet: The mice were inoculated with purified, formalin-killed NMII C. burnetii mixed with the monophosphoryl lipid A-trehalose dicorynomycolate emulsion adjuvant system (Sigma-Aldrich, St. Louis, MO) and boosted according to the manufacturer's protocol. .. Briefly, C. burnetii NMII cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an Immobilon-P membrane (Millipore, Bedford, MA).

    Affinity Chromatography:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma). .. The recombinant protein was purified by affinity chromatography using precharged Ni-NTA Sepharose column (Qiagen) and refolded when dialyzed in PBS using Slide-A-Lyzer (Pierce) according to the manufacturer's recommendations.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Role of protein kinase C ? and vascular endothelial growth factor receptor in malignant pleural mesothelioma: Therapeutic implications and the usefulness of Caenorhabditis elegans model organism
    Article Snippet: .. Protein lysates (60–80 µ g) were separated by 7.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred to PVDF membranes (Millipore, Bedford, MA, USA). ..

    Article Title: A host cell membrane protein, golgin-97, is essential for poxvirus morphogenesis
    Article Snippet: .. Proteins were separated by sodium dodecyl sulfate (SDS) – polyacrylamide gel electrophoresis (PAGE) and transferred to PVDF-membrane (Immobilon-PSQ , Millipore, Billerica, MA). .. The membrane was blocked in 3% gelatin in TTBS (0.05% Tween 20– 150mM NaCl - 50mM TrisHCl, pH 7.4) and incubated with primary anti-golgin-97 (1 μg/ml) or anti-actin antibodies (1:1000) in 1% gelatin - TTBS overnight.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata: Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: .. Purification and Determination of the Partial Amino Acid Sequence of the 47 kDa Protein To determine the N-terminal amino acid sequence of this 47 kDa protein, proteins of the urea-soluble fraction dissolved in 30 mM Tris-HCl (pH 8.0) were separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinylidene fluoride membrane (Millipore) using a dry blotting system (Nippon Eido). .. After Ponceau S staining, the band was cut out and then subjected to N-terminal amino acid sequence analysis.

    Article Title: MEG3 Activated by Vitamin D Inhibits Colorectal Cancer Cells Proliferation and Migration via Regulating Clusterin
    Article Snippet: .. 20 μg total proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. Membranes were blocked in 5% non-fat milk in Tris-buffered saline with 0.05% Tween-20 at room temperature for 2 h and then probed with antibodies against Clusterin (sc-6420; RRID: AB_2083323 ; Santa Cruz, CA, 1:200 dilution) and beta-actin (Abcam Cat# 1854–1, RRID: AB_764434 , 1:1000 dilution), and probed with goat anti-rabbit secondary antibody conjugated with horseradish peroxidase (Abcam Cat# ab7088, RRID: AB_955595 ).

    Article Title: Activation of mineralocorticoid receptor by ecdysone, an adaptogenic and anabolic ecdysteroid, promotes glomerular injury and proteinuria involving overactive GSK3β pathway signaling
    Article Snippet: .. To discern the composition of urine proteins, urine samples were processed for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with Coomassie Brilliant Blue (Sigma-Aldrich, St. Louis, MO). .. Mice were anesthetized and perfused by infusing the abdominal artery with 5 ml of PBS containing 8 × 107 Dynabeads M-450 (Dynal Biotech ASA, Oslo, Norway).

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature. .. Reactive bands were developed and enhanced by SuperSignal West Pico chemiluminescent detection reagents according to the instructions of the manufacturer (Thermo Scientific, Rockford, IL, USA).

    Lysis:

    Article Title: Role of protein kinase C ? and vascular endothelial growth factor receptor in malignant pleural mesothelioma: Therapeutic implications and the usefulness of Caenorhabditis elegans model organism
    Article Snippet: The cells were washed twice with ice-cold phosphate buffered saline (PBS), and whole cell lysates were prepared in RIPA lysis buffer [50 mM Tris (pH 8.0), 150 mM NaCl, 10% glycerol, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and 0.42% NaF containing protease inhibitor cocktail (1 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , 5 mg/ml leupeptin)]. .. Protein lysates (60–80 µ g) were separated by 7.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred to PVDF membranes (Millipore, Bedford, MA, USA).

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: The cell lysates in NP-40 lysis buffer were further sonicated and centrifuged to obtain fractions containing soluble supernatants and insoluble pellets. .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature.

    Mouse Assay:

    Article Title: Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii ▿
    Article Snippet: The mice were inoculated with purified, formalin-killed NMII C. burnetii mixed with the monophosphoryl lipid A-trehalose dicorynomycolate emulsion adjuvant system (Sigma-Aldrich, St. Louis, MO) and boosted according to the manufacturer's protocol. .. Briefly, C. burnetii NMII cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an Immobilon-P membrane (Millipore, Bedford, MA).

    SDS Page:

    Article Title: Role of protein kinase C ? and vascular endothelial growth factor receptor in malignant pleural mesothelioma: Therapeutic implications and the usefulness of Caenorhabditis elegans model organism
    Article Snippet: .. Protein lysates (60–80 µ g) were separated by 7.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred to PVDF membranes (Millipore, Bedford, MA, USA). ..

    Article Title: Distinct Domains in the Adenovirus E3 RID? Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor
    Article Snippet: .. Membrane pellets were solubilized in 2× Laemmli buffer, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to Immobilon-P membranes (Millipore, Bedford, Mass.). .. For analysis of Fas degradation, proteins solubilized at 18 h postinfection (p.i.) in radioimmunoprecipitation assay buffer were transferred to Immobilon-P. Rabbit antiserum P77-91, which was raised against a synthetic peptide corresponding to amino acids 77 to 91 of RIDα, was used at a dilution of 1:200.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata: Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: .. Purification and Determination of the Partial Amino Acid Sequence of the 47 kDa Protein To determine the N-terminal amino acid sequence of this 47 kDa protein, proteins of the urea-soluble fraction dissolved in 30 mM Tris-HCl (pH 8.0) were separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinylidene fluoride membrane (Millipore) using a dry blotting system (Nippon Eido). .. After Ponceau S staining, the band was cut out and then subjected to N-terminal amino acid sequence analysis.

    Article Title: Adenylyl cyclase type 6 overexpression selectively enhances ?-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts
    Article Snippet: .. Equal volumes of fractions were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Millipore) by electroblotting. .. Membranes were blocked in PBS–Tween with 5% nonfat dry milk and incubated with primary antibody (see “ ”) overnight at 4°C.

    Article Title: MEG3 Activated by Vitamin D Inhibits Colorectal Cancer Cells Proliferation and Migration via Regulating Clusterin
    Article Snippet: .. 20 μg total proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. Membranes were blocked in 5% non-fat milk in Tris-buffered saline with 0.05% Tween-20 at room temperature for 2 h and then probed with antibodies against Clusterin (sc-6420; RRID: AB_2083323 ; Santa Cruz, CA, 1:200 dilution) and beta-actin (Abcam Cat# 1854–1, RRID: AB_764434 , 1:1000 dilution), and probed with goat anti-rabbit secondary antibody conjugated with horseradish peroxidase (Abcam Cat# ab7088, RRID: AB_955595 ).

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: .. The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma). .. The recombinant protein was purified by affinity chromatography using precharged Ni-NTA Sepharose column (Qiagen) and refolded when dialyzed in PBS using Slide-A-Lyzer (Pierce) according to the manufacturer's recommendations.

    Article Title: Activation of mineralocorticoid receptor by ecdysone, an adaptogenic and anabolic ecdysteroid, promotes glomerular injury and proteinuria involving overactive GSK3β pathway signaling
    Article Snippet: .. To discern the composition of urine proteins, urine samples were processed for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with Coomassie Brilliant Blue (Sigma-Aldrich, St. Louis, MO). .. Mice were anesthetized and perfused by infusing the abdominal artery with 5 ml of PBS containing 8 × 107 Dynabeads M-450 (Dynal Biotech ASA, Oslo, Norway).

    Article Title: Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii ▿
    Article Snippet: .. Briefly, C. burnetii NMII cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an Immobilon-P membrane (Millipore, Bedford, MA). .. The membrane was placed in a Miniblotter 25 multichannel screening apparatus (Immunetics, Cambridge, MA) and incubated with undiluted hybridoma culture supernatants.

    Article Title: The Study of the Intercellular Trafficking of the Fusion Proteins of Herpes Simplex Virus Protein VP22
    Article Snippet: .. Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.22 µm PVDF membrane (EMD Millipore, Billerica, MA, USA) and probed with the first antibodies at 4°C overnight, then washed and incubated with horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature. .. Reactive bands were developed and enhanced by SuperSignal West Pico chemiluminescent detection reagents according to the instructions of the manufacturer (Thermo Scientific, Rockford, IL, USA).

    Plasmid Preparation:

    Article Title: Generation of affibody molecules specific for HPV16 E7 recognition
    Article Snippet: Expression and purification of HPV16 E7-binding affibody molecules The sequences of selected HPV16 E7-binding affibodies molecules, including ZHPV16 E7 127, ZHPV16 E7 301, ZHPV16 E7 384, ZHPV16 E7 745 and wild SPA-Z scaffold (Z-WT ), were subcloned into the Nde I and EcoRI sites of pET21a(+) expression vector. .. The 6×His-tagged recombinant proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and confirmed by Western blotting using anti-His antibody (Sigma).

    Software:

    Article Title: Regulation of Phosphoinositide 3-Kinase by Its Intrinsic Serine Kinase Activity In Vivo
    Article Snippet: Isolated protein or immunoprecipitates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon P; Millipore). .. Images were analyzed with a Fuji LAS-1000 Luminescent Image Analyser and Fuji Image Gauge software.

    Binding Assay:

    Article Title: A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example
    Article Snippet: Proteins are resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred of proteins from gel to NC membrane (Millipore). .. NC Membranes were blocked for 4 h with 5% nonfat dry milk solution in Tris-buffered saline and as thorough removal of the unbound reagents after each of the binding steps in the procedure.

    Radio Immunoprecipitation:

    Article Title: Distinct Domains in the Adenovirus E3 RID? Protein Are Required for Degradation of Fas and the Epidermal Growth Factor Receptor
    Article Snippet: Membrane pellets were solubilized in 2× Laemmli buffer, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to Immobilon-P membranes (Millipore, Bedford, Mass.). .. For analysis of Fas degradation, proteins solubilized at 18 h postinfection (p.i.) in radioimmunoprecipitation assay buffer were transferred to Immobilon-P. Rabbit antiserum P77-91, which was raised against a synthetic peptide corresponding to amino acids 77 to 91 of RIDα, was used at a dilution of 1:200.

    Quantitation Assay:

    Article Title: Activation of mineralocorticoid receptor by ecdysone, an adaptogenic and anabolic ecdysteroid, promotes glomerular injury and proteinuria involving overactive GSK3β pathway signaling
    Article Snippet: The concentrations of albumin in urine samples were measured by using mouse albumin ELISA quantitation kit (Bethyl Laboratories Inc., Montgomery, TX). .. To discern the composition of urine proteins, urine samples were processed for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with Coomassie Brilliant Blue (Sigma-Aldrich, St. Louis, MO).

    Spectrophotometry:

    Article Title: A comprehensive procedure for antiviral inhibitor discovery using EV71 as an example
    Article Snippet: Western blot analysis To monitor the inhibition of virally encoded protein by compounds, we infect the host cells with virus for 24 or 48 h. Cells are then lysed in a buffer containing 50 mmol/L Tris-HCl, pH 8.0, 150 mmol/L NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mmol/L EDTA, 1 mmol/L NaVO4 , 10 mmol/L NaF, and protease inhibitors, and the protein concentration in the lysates was determined by a spectrophotometer. .. Proteins are resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred of proteins from gel to NC membrane (Millipore).

    Produced:

    Article Title: Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii ▿
    Article Snippet: Monoclonal antibodies (MAb) were produced from hybridomas resulting from fusion of spleen cells from female BALB/c mice and SP2/0 myeloma cells. .. Briefly, C. burnetii NMII cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to an Immobilon-P membrane (Millipore, Bedford, MA).

    Fractionation:

    Article Title: Adenylyl cyclase type 6 overexpression selectively enhances ?-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts
    Article Snippet: Paragraph title: Membrane fractionation ... Equal volumes of fractions were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Millipore) by electroblotting.

    Staining:

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata: Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: Purification and Determination of the Partial Amino Acid Sequence of the 47 kDa Protein To determine the N-terminal amino acid sequence of this 47 kDa protein, proteins of the urea-soluble fraction dissolved in 30 mM Tris-HCl (pH 8.0) were separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted onto a polyvinylidene fluoride membrane (Millipore) using a dry blotting system (Nippon Eido). .. After Ponceau S staining, the band was cut out and then subjected to N-terminal amino acid sequence analysis.

    Article Title: Activation of mineralocorticoid receptor by ecdysone, an adaptogenic and anabolic ecdysteroid, promotes glomerular injury and proteinuria involving overactive GSK3β pathway signaling
    Article Snippet: .. To discern the composition of urine proteins, urine samples were processed for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with Coomassie Brilliant Blue (Sigma-Aldrich, St. Louis, MO). .. Mice were anesthetized and perfused by infusing the abdominal artery with 5 ml of PBS containing 8 × 107 Dynabeads M-450 (Dynal Biotech ASA, Oslo, Norway).

    Electrochemiluminescence:

    Article Title: Adenylyl cyclase type 6 overexpression selectively enhances ?-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts
    Article Snippet: Equal volumes of fractions were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Millipore) by electroblotting. .. Bound primary antibodies were visualized using an appropriate secondary antibody with conjugated horseradish peroxidase (Santa Cruz Biotechnology) and electrochemiluminescence reagent (Pierce).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore polyvinylidene difluoride pvdf membranes
    Polyvinylidene Difluoride Pvdf Membranes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyvinylidene difluoride pvdf membranes/product/Millipore
    Average 99 stars, based on 1753 article reviews
    Price from $9.99 to $1999.99
    polyvinylidene difluoride pvdf membranes - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results