sodium dodecyl sulfate proteinase k  (Millipore)


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    Name:
    Sodium dodecyl sulfate
    Description:

    Catalog Number:
    l5750
    Price:
    None
    Applications:
    Sodium dodecyl sulfate has been used:. to dissolve insoluble purple formazan in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. to perform SDS-PAGE of yeast tubulin. as an additive in linolenic acid solution to assess the total antioxidative activity (TAA) of the bacteria using the linolenic acid test (LA-test)
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    Millipore sodium dodecyl sulfate proteinase k
    Sodium dodecyl sulfate

    https://www.bioz.com/result/sodium dodecyl sulfate proteinase k/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium dodecyl sulfate proteinase k - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Betulinic Acid Suppresses STAT3 Activation Pathway Through Induction of Protein Tyrosine Phosphatase SHP-1 in Human Multiple Myeloma Cells
    Article Snippet: We purchased Hoechst 33342, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Tris, glycine, NaCl, sodium dodecyl sulfate (SDS), and bovine serum albumin from Sigma-Aldrich (St. Louis, MO), and we obtained Roswell Park Memorial Institute (RPMI) medium 1640, fetal bovine serum (FBS), 0.4% trypan blue vital stain, and antibiotic-antimycotic mixture from Life Technologies (Grand Island, NY). .. We also purchased antibodies to phospho-specific Src (Tyr416), Src, phospho-specific JAK1 (Tyr1022/1023), JAK1, and JAK2 from Cell Signaling Technology (Beverly, MA), goat anti-mouse HRP from Transduction Laboratories (Lexington, KY), and goat anti-rabbit Alexa 594 from Molecular Probes (Eugene, OR).

    De-Phosphorylation Assay:

    Article Title: Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity from Prevotella intermedia
    Article Snippet: Paragraph title: Dephosphorylation of phosphotyrosine proteins in A431 lysate. ... A 2-μl aliquot of purified PiACP was incubated with 10 μl of lysate of the human epidermoid carcinoma cell line A431 that contained phosphotyrosine EGFR (Upstate Biotechnology) in the presence of 50 mM sodium acetate (pH 4.9)–50 mM NaCl–5% glycerol at 37°C for 1, 3, or 15 h. Proteins were resolved in 10% polyacrylamide gels containing sodium dodecyl sulfate (SDS) , followed by transfer to Immobilon-P membranes (Millipore) for immunoblot (Western) analysis by using RC20H antibodies (1:1,000) and the ECL method (Amersham).

    Stable Transfection:

    Article Title: Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a
    Article Snippet: Bacterial growth medium, dodecyltrimethylammonium chloride (DTAC), sodium dodecyl sulfate (SDS), reduced and oxidized glutathione were from Sigma-Aldrich. .. Murine Wnt3a was expressed in stably transfected Drosophila S2 cells and isolated from conditioned media according to [ ].

    Enzyme-linked Immunosorbent Assay:

    Article Title: Human L-ferritin deficiency is characterized by idiopathic generalized seizures and atypical restless leg syndrome
    Article Snippet: Ferritin levels were determined by ELISA using the monoclonal antibodies L03 (specific for the FtL) and rH02 (specific for the FtH) calibrated on the corresponding recombinant homopolymer ( ). .. 20 µg of soluble proteins were separated by sodium dodecyl sulfate–PAGE (SDS-PAGE), and immunoblotting was performed using specific antibodies for Catalase (EMD Millipore), SOD1 (Santa Cruz Biotechnology, Inc.), SOD2 (Santa Cruz Biotechnology, Inc.), TfR1 (Zymed), and β-actin (Sigma-Aldrich), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Acrylamide Gel Assay:

    Article Title: Evidence for Transcriptional and Posttranscriptional Alterations of the Sodium/Iodide Symporter Expression in Hypofunctioning Benign and Malignant Thyroid Tumors
    Article Snippet: .. Membrane proteins (1 to 180 μg of protein) were subjected to electrophoresis on 7.5% acrylamide gel in the presence of sodium dodecyl sulfate (SDS) and electrotransferred onto Immobilon P membranes (Millipore Corp., Bedford, MA). .. Membranes were preincubated in PBS supplemented with 5% nonfat dry milk and 0.2% Tween 20 for 1 hour at room temperature and then incubated overnight at 4°C with the IgG fraction from pAb 795 immune serum (5 to 30 μg/ml) in the same buffer.

    Incubation:

    Article Title: Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity from Prevotella intermedia
    Article Snippet: .. A 2-μl aliquot of purified PiACP was incubated with 10 μl of lysate of the human epidermoid carcinoma cell line A431 that contained phosphotyrosine EGFR (Upstate Biotechnology) in the presence of 50 mM sodium acetate (pH 4.9)–50 mM NaCl–5% glycerol at 37°C for 1, 3, or 15 h. Proteins were resolved in 10% polyacrylamide gels containing sodium dodecyl sulfate (SDS) , followed by transfer to Immobilon-P membranes (Millipore) for immunoblot (Western) analysis by using RC20H antibodies (1:1,000) and the ECL method (Amersham). ..

    Article Title: Evidence for Transcriptional and Posttranscriptional Alterations of the Sodium/Iodide Symporter Expression in Hypofunctioning Benign and Malignant Thyroid Tumors
    Article Snippet: Membrane proteins (1 to 180 μg of protein) were subjected to electrophoresis on 7.5% acrylamide gel in the presence of sodium dodecyl sulfate (SDS) and electrotransferred onto Immobilon P membranes (Millipore Corp., Bedford, MA). .. Membranes were preincubated in PBS supplemented with 5% nonfat dry milk and 0.2% Tween 20 for 1 hour at room temperature and then incubated overnight at 4°C with the IgG fraction from pAb 795 immune serum (5 to 30 μg/ml) in the same buffer.

    Expressing:

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich). .. Band intensity was revealed by enhanced chemiluminescence (ECL; GE Healthcare).

    BIA-KA:

    Article Title: Human L-ferritin deficiency is characterized by idiopathic generalized seizures and atypical restless leg syndrome
    Article Snippet: Protein concentration was evaluated by the bicinchoninic acid (BCA) method (Thermo Fisher Scientific) calibrated on BSA. .. 20 µg of soluble proteins were separated by sodium dodecyl sulfate–PAGE (SDS-PAGE), and immunoblotting was performed using specific antibodies for Catalase (EMD Millipore), SOD1 (Santa Cruz Biotechnology, Inc.), SOD2 (Santa Cruz Biotechnology, Inc.), TfR1 (Zymed), and β-actin (Sigma-Aldrich), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: Protein concentration was evaluated by the bicinchoninic acid (BCA) method (Pierce) calibrated on BSA. .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Modification:

    Article Title: Expanding Proteome Coverage with Orthogonal-specificity α-Lytic Proteases *
    Article Snippet: N-ethyl maleimide (NEM), formic acid (FA), sodium deoxycholate (SDC), sodium dodecyl sulfate (SDS), and Trizma-brand Tris buffer were obtained from Sigma-Aldrich (St. Louis, MO). .. Sequencing-grade modified trypsin was purchased from Promega (Madison, WI).

    Western Blot:

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: The expression of the transgene was also confirmed by western blotting. .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Article Title: Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity from Prevotella intermedia
    Article Snippet: .. A 2-μl aliquot of purified PiACP was incubated with 10 μl of lysate of the human epidermoid carcinoma cell line A431 that contained phosphotyrosine EGFR (Upstate Biotechnology) in the presence of 50 mM sodium acetate (pH 4.9)–50 mM NaCl–5% glycerol at 37°C for 1, 3, or 15 h. Proteins were resolved in 10% polyacrylamide gels containing sodium dodecyl sulfate (SDS) , followed by transfer to Immobilon-P membranes (Millipore) for immunoblot (Western) analysis by using RC20H antibodies (1:1,000) and the ECL method (Amersham). ..

    Article Title: Evidence for Transcriptional and Posttranscriptional Alterations of the Sodium/Iodide Symporter Expression in Hypofunctioning Benign and Malignant Thyroid Tumors
    Article Snippet: Paragraph title: Western Blot Analyses ... Membrane proteins (1 to 180 μg of protein) were subjected to electrophoresis on 7.5% acrylamide gel in the presence of sodium dodecyl sulfate (SDS) and electrotransferred onto Immobilon P membranes (Millipore Corp., Bedford, MA).

    High Performance Liquid Chromatography:

    Article Title: Expanding Proteome Coverage with Orthogonal-specificity α-Lytic Proteases *
    Article Snippet: Acetonitrile (ACN) optima, tri-carboxyethyl phosphine (Bond-Breaker), and HPLC-grade ethyl-acetate were purchased from Thermo Fisher Scientific (Waltham, MA). .. N-ethyl maleimide (NEM), formic acid (FA), sodium deoxycholate (SDC), sodium dodecyl sulfate (SDS), and Trizma-brand Tris buffer were obtained from Sigma-Aldrich (St. Louis, MO).

    Article Title: APols-Aided Protein Precipitation: A Rapid Method for Concentrating Proteins for Proteomic Analysis
    Article Snippet: Urea, dithiothreitol (DTT), iodoacetamide (IAA), ammonium bicarbonate (ABC), formic acid (FA), and sodium dodecyl sulfate (SDS) were obtained from Sigma Aldrich (St. Louis, MO, USA). .. Water and acetonitrile (ACN) for HPLC were obtained from JT Baker (Phillipsburg NJ, USA).

    Transfection:

    Article Title: Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a
    Article Snippet: Bacterial growth medium, dodecyltrimethylammonium chloride (DTAC), sodium dodecyl sulfate (SDS), reduced and oxidized glutathione were from Sigma-Aldrich. .. Murine Wnt3a was expressed in stably transfected Drosophila S2 cells and isolated from conditioned media according to [ ].

    Cell Culture:

    Article Title: Betulinic Acid Suppresses STAT3 Activation Pathway Through Induction of Protein Tyrosine Phosphatase SHP-1 in Human Multiple Myeloma Cells
    Article Snippet: We prepared a 50-mM solution of betulinic acid (purity > 98%; Alexis, San Diego, CA) in ethanol, stored the solution as small aliquots at −20°C, and then diluted the solution as needed in cell culture medium. .. We purchased Hoechst 33342, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Tris, glycine, NaCl, sodium dodecyl sulfate (SDS), and bovine serum albumin from Sigma-Aldrich (St. Louis, MO), and we obtained Roswell Park Memorial Institute (RPMI) medium 1640, fetal bovine serum (FBS), 0.4% trypan blue vital stain, and antibiotic-antimycotic mixture from Life Technologies (Grand Island, NY).

    other:

    Article Title: Salmonella enterica Serovar Typhimurium Swarming Mutants with Altered Biofilm-Forming Abilities: Surfactin Inhibits Biofilm Formation
    Article Snippet: Surfactin, sodium dodecyl sulfate (SDS), and Tween 80 were purchased from Sigma Chemical Co. Rhamnolipid (extracted from Pseudomonas aeruginosa ) was a gift from Jeneil Biosurfactant Co. Inc. PVC 96-well microtiter plates were from Costar (2596; nontreated).

    Protein Concentration:

    Article Title: Human L-ferritin deficiency is characterized by idiopathic generalized seizures and atypical restless leg syndrome
    Article Snippet: Protein concentration was evaluated by the bicinchoninic acid (BCA) method (Thermo Fisher Scientific) calibrated on BSA. .. 20 µg of soluble proteins were separated by sodium dodecyl sulfate–PAGE (SDS-PAGE), and immunoblotting was performed using specific antibodies for Catalase (EMD Millipore), SOD1 (Santa Cruz Biotechnology, Inc.), SOD2 (Santa Cruz Biotechnology, Inc.), TfR1 (Zymed), and β-actin (Sigma-Aldrich), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: Protein concentration was evaluated by the bicinchoninic acid (BCA) method (Pierce) calibrated on BSA. .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Sequencing:

    Article Title: Expanding Proteome Coverage with Orthogonal-specificity α-Lytic Proteases *
    Article Snippet: N-ethyl maleimide (NEM), formic acid (FA), sodium deoxycholate (SDC), sodium dodecyl sulfate (SDS), and Trizma-brand Tris buffer were obtained from Sigma-Aldrich (St. Louis, MO). .. Sequencing-grade modified trypsin was purchased from Promega (Madison, WI).

    Recombinant:

    Article Title: Human L-ferritin deficiency is characterized by idiopathic generalized seizures and atypical restless leg syndrome
    Article Snippet: Ferritin levels were determined by ELISA using the monoclonal antibodies L03 (specific for the FtL) and rH02 (specific for the FtH) calibrated on the corresponding recombinant homopolymer ( ). .. 20 µg of soluble proteins were separated by sodium dodecyl sulfate–PAGE (SDS-PAGE), and immunoblotting was performed using specific antibodies for Catalase (EMD Millipore), SOD1 (Santa Cruz Biotechnology, Inc.), SOD2 (Santa Cruz Biotechnology, Inc.), TfR1 (Zymed), and β-actin (Sigma-Aldrich), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Article Title: Betulinic Acid Suppresses STAT3 Activation Pathway Through Induction of Protein Tyrosine Phosphatase SHP-1 in Human Multiple Myeloma Cells
    Article Snippet: We purchased Hoechst 33342, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Tris, glycine, NaCl, sodium dodecyl sulfate (SDS), and bovine serum albumin from Sigma-Aldrich (St. Louis, MO), and we obtained Roswell Park Memorial Institute (RPMI) medium 1640, fetal bovine serum (FBS), 0.4% trypan blue vital stain, and antibiotic-antimycotic mixture from Life Technologies (Grand Island, NY). .. We obtained bacteria-derived recombinant human IL-6 from Novartis Pharmaceuticals (East Hanover, NJ).

    DC Protein Assay:

    Article Title: APols-Aided Protein Precipitation: A Rapid Method for Concentrating Proteins for Proteomic Analysis
    Article Snippet: Urea, dithiothreitol (DTT), iodoacetamide (IAA), ammonium bicarbonate (ABC), formic acid (FA), and sodium dodecyl sulfate (SDS) were obtained from Sigma Aldrich (St. Louis, MO, USA). .. DC protein assay kit II (500-0112) was purchased from Bio-Rad.

    MTT Assay:

    Article Title: Hydrolysis of Interleukin-12 by Porphyromonas gingivalis Major Cysteine Proteinases May Affect Local Gamma Interferon Accumulation and the Th1 or Th2 T-Cell Phenotype in Periodontitis
    Article Snippet: .. Collagenase type 1A, endothelial cell growth factor, leupeptin, l -arginine, l -lysine, l -cysteine, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), N -α-tosyl- l -lysyl chloromethyl ketone (TLCK), phytohemagglutinin (PHA), polymyxin B, propanol, sodium dodecyl sulfate (SDS), tosyl-Gly- l -Pro- l -Arg p -nitroanilide, tosyl-Gly- l -Pro- l -Lys p -nitroanilide, Trizma base, Tris-HCl, and trypsin were purchased from Sigma (St. Louis, Mo.). .. Trypticase soy broth was purchased from Difco (Detroit, Mich.).

    Article Title: Betulinic Acid Suppresses STAT3 Activation Pathway Through Induction of Protein Tyrosine Phosphatase SHP-1 in Human Multiple Myeloma Cells
    Article Snippet: .. We purchased Hoechst 33342, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Tris, glycine, NaCl, sodium dodecyl sulfate (SDS), and bovine serum albumin from Sigma-Aldrich (St. Louis, MO), and we obtained Roswell Park Memorial Institute (RPMI) medium 1640, fetal bovine serum (FBS), 0.4% trypan blue vital stain, and antibiotic-antimycotic mixture from Life Technologies (Grand Island, NY). .. We obtained rabbit polyclonal STAT3 antibodies and mouse monoclonal antibodies against phospho-STAT3 (Tyr705), bcl-2, bcl-xL, SHP-1, cyclin D1, procaspase-3, and poly(ADP-ribose) polymerase (PARP) from Santa Cruz Biotechnology (Santa Cruz, CA), and we purchased goat anti-rabbit horseradish peroxidase (HRP) conjugate from Bio-Rad (Hercules, CA).

    Isolation:

    Article Title: Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a
    Article Snippet: Bacterial growth medium, dodecyltrimethylammonium chloride (DTAC), sodium dodecyl sulfate (SDS), reduced and oxidized glutathione were from Sigma-Aldrich. .. Murine Wnt3a was expressed in stably transfected Drosophila S2 cells and isolated from conditioned media according to [ ].

    Immunodetection:

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: Paragraph title: Ferritin quantification and protein immunodetection ... The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Purification:

    Article Title: Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity from Prevotella intermedia
    Article Snippet: .. A 2-μl aliquot of purified PiACP was incubated with 10 μl of lysate of the human epidermoid carcinoma cell line A431 that contained phosphotyrosine EGFR (Upstate Biotechnology) in the presence of 50 mM sodium acetate (pH 4.9)–50 mM NaCl–5% glycerol at 37°C for 1, 3, or 15 h. Proteins were resolved in 10% polyacrylamide gels containing sodium dodecyl sulfate (SDS) , followed by transfer to Immobilon-P membranes (Millipore) for immunoblot (Western) analysis by using RC20H antibodies (1:1,000) and the ECL method (Amersham). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich). .. Band intensity was revealed by enhanced chemiluminescence (ECL; GE Healthcare).

    SDS Page:

    Article Title: Human L-ferritin deficiency is characterized by idiopathic generalized seizures and atypical restless leg syndrome
    Article Snippet: .. 20 µg of soluble proteins were separated by sodium dodecyl sulfate–PAGE (SDS-PAGE), and immunoblotting was performed using specific antibodies for Catalase (EMD Millipore), SOD1 (Santa Cruz Biotechnology, Inc.), SOD2 (Santa Cruz Biotechnology, Inc.), TfR1 (Zymed), and β-actin (Sigma-Aldrich), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich). .. Band intensity was revealed by enhanced chemiluminescence (ECL; GE Healthcare).

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich). .. Band intensity was revealed by enhanced chemiluminescence (ECL; GE Healthcare).

    Electrophoresis:

    Article Title: Evidence for Transcriptional and Posttranscriptional Alterations of the Sodium/Iodide Symporter Expression in Hypofunctioning Benign and Malignant Thyroid Tumors
    Article Snippet: .. Membrane proteins (1 to 180 μg of protein) were subjected to electrophoresis on 7.5% acrylamide gel in the presence of sodium dodecyl sulfate (SDS) and electrotransferred onto Immobilon P membranes (Millipore Corp., Bedford, MA). .. Membranes were preincubated in PBS supplemented with 5% nonfat dry milk and 0.2% Tween 20 for 1 hour at room temperature and then incubated overnight at 4°C with the IgG fraction from pAb 795 immune serum (5 to 30 μg/ml) in the same buffer.

    Concentration Assay:

    Article Title: Broad-Spectrum Antimicrobial Activity and Low Cytotoxicity against Human Cells of a Peptide Derived from Bovine αS1-Casein
    Article Snippet: Before assessments, peptide was liquefied in deionized water at a concentration of 2.56 mM. .. Sodium dodecyl sulfate (SDS), trifluoroethyl alcohol (TFE), Triton X-100, o-nitrophenyl-b-d-galactopyranoside (ONPG), 3,30-dipropylthiadicarbocyanine (diSC3-5), HEPES, N -phenyl-1-napthylamine (NPN), propidium iodide (PI) were obtained from Sigma-Aldrich (Shanghai, China).

    Article Title: Evidence for Transcriptional and Posttranscriptional Alterations of the Sodium/Iodide Symporter Expression in Hypofunctioning Benign and Malignant Thyroid Tumors
    Article Snippet: Frozen tissue samples were rapidly weighed and homogenized in ice-cold phosphate-buffered saline (PBS) (1 ml/100 mg tissue) supplemented with protease inhibitors (apoprotinin, peptasin, leupeptin, each at a concentration of 1 μg/ml) using a Teflon-glass Potter homogenizer. .. Membrane proteins (1 to 180 μg of protein) were subjected to electrophoresis on 7.5% acrylamide gel in the presence of sodium dodecyl sulfate (SDS) and electrotransferred onto Immobilon P membranes (Millipore Corp., Bedford, MA).

    Staining:

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: To study iron incorporation capacity, equal amounts of the 70 °C heated samples were separated on native-PAGE and then the gels were stained with Prussian Blue and enhanced with 0.025% 3,3′-diaminobenzidine (DAB) and 0.05% H2 O2 for 15 min. .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

    Article Title: Dilution of protein-surfactant complexes: A fluorescence study
    Article Snippet: Bovine serum albumin (BSA, 66 kDa), beta-galactosidase from E. coli (betagal, 465 kDa), carbonic anhydrase from bovine erythrocytes (CA, 28 kDa), sodium dodecyl sulfate (SDS), hexadecyl trimethyl ammonium bromide (CTAB), beta-mercaptoethanol (DTT), and Tris–Glycine buffer were obtained from Sigma (St. Louis, MO). .. Sypro Orange protein staining dye (5000×) was purchased from Invitrogen (Carlsbad, CA).

    Article Title: Betulinic Acid Suppresses STAT3 Activation Pathway Through Induction of Protein Tyrosine Phosphatase SHP-1 in Human Multiple Myeloma Cells
    Article Snippet: .. We purchased Hoechst 33342, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Tris, glycine, NaCl, sodium dodecyl sulfate (SDS), and bovine serum albumin from Sigma-Aldrich (St. Louis, MO), and we obtained Roswell Park Memorial Institute (RPMI) medium 1640, fetal bovine serum (FBS), 0.4% trypan blue vital stain, and antibiotic-antimycotic mixture from Life Technologies (Grand Island, NY). .. We obtained rabbit polyclonal STAT3 antibodies and mouse monoclonal antibodies against phospho-STAT3 (Tyr705), bcl-2, bcl-xL, SHP-1, cyclin D1, procaspase-3, and poly(ADP-ribose) polymerase (PARP) from Santa Cruz Biotechnology (Santa Cruz, CA), and we purchased goat anti-rabbit horseradish peroxidase (HRP) conjugate from Bio-Rad (Hercules, CA).

    Clear Native PAGE:

    Article Title: A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits
    Article Snippet: To study iron incorporation capacity, equal amounts of the 70 °C heated samples were separated on native-PAGE and then the gels were stained with Prussian Blue and enhanced with 0.025% 3,3′-diaminobenzidine (DAB) and 0.05% H2 O2 for 15 min. .. The expression of other proteins was assayed by analyzing 20 μg of homogenate soluble proteins separated by sodium dodecyl sulfate -PAGE (SDS-PAGE) and immunoblotting was performed using specific antibodies for Catalase (EMD, Millipore), TfR1 (Zymed), Ubiquitin (Santa Cruz Biotechnology) and β-actin (Sigma), followed by peroxidase-labeled secondary antibodies (Sigma-Aldrich).

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    Millipore polyvinylidene difluoride pvdf membranes
    Polyvinylidene Difluoride Pvdf Membranes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyvinylidene difluoride pvdf membranes/product/Millipore
    Average 99 stars, based on 1753 article reviews
    Price from $9.99 to $1999.99
    polyvinylidene difluoride pvdf membranes - by Bioz Stars, 2020-04
    99/100 stars
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