sodium diphosphate buffer  (Millipore)


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  • 99
    Name:
    pH 4 Buffer
    Description:
    This Certified Reference Material CRM is produced and certified in accordance with ISO 17034 and ISO IEC 17025 All information regarding the use of this CRM can be found on the certificate of analysis
    Catalog Number:
    qc1121
    Price:
    None
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    Structured Review

    Millipore sodium diphosphate buffer
    This Certified Reference Material CRM is produced and certified in accordance with ISO 17034 and ISO IEC 17025 All information regarding the use of this CRM can be found on the certificate of analysis
    https://www.bioz.com/result/sodium diphosphate buffer/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium diphosphate buffer - by Bioz Stars, 2020-09
    99/100 stars

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    Lysis:

    Article Title: THE ACTIN BUNDLING PROTEIN PALLADIN IS AN AKT1-SPECIFIC SUBSTRATE THAT REGULATES BREAST CANCER CELL MIGRATION
    Article Snippet: .. Cells were lysed 24 h post-transfection in ice-cold lysis buffer (1% NP-40, 150 mM NaCl, 10 mM KCl, 20 mM Tris-HCl (pH 7.5), 0.1% SDC, 0.1% SDS, proteinase inhibitor cocktail (Sigma-Aldrich), 50 nM calyculin (Sigma-Aldrich), 1 mM sodium pyrophosphate, 20 mM sodium fluoride). .. Actin binding and bundling assays were performed according to the manufacturer’s protocol (Cytoskeleton).

    Article Title: Expression, purification and kinetic characterization of recombinant benzoate dioxygenase from Rhodococcus ruber UKMP-5M
    Article Snippet: .. The pellet was washed and resuspended in a lysis buffer (50 mM NaH2PO4 with 300 mM NaCl) (pH 8.0) containing 1 μg/mL lysozyme, and 0.1 mM/mL phenylmethyl sulfonyl fluoride (PMSF) (Sigma) was added to protect the protein structure. ..

    Article Title: Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state
    Article Snippet: .. Next day upon transfection, cells were lysed in lysis buffer (1% NP-40, 150 mM NaCl, and 10 mM Tris [pH 7.4]) containing protease inhibitor (Sigma-Aldrich). .. A small fraction of the cell lysate was analyzed by western blotting using rat anti-kindlin-3 antibody (1:1,000 dilution) (clone 181A, generated in-house).

    Article Title: Phase I/II Study of Combination Therapy With Sorafenib, Idarubicin, and Cytarabine in Younger Patients With Acute Myeloid Leukemia
    Article Snippet: .. The cells were washed twice with ice-cold phosphate-buffered saline and then lysed by resuspending them in lysis buffer (20 mmol/L Tris pH 7.4, 100 mmol/L NaCl, 1% Igepal (Sigma, St. Louis, MO), 1 mmol/L ethylenediaminetetra-acetate, 2 mmol/L NaVO4, plus Complete Protease Inhibitor (Roche, Indianapolis, IN) for 30 minutes while rocking. .. Anti-FLT3 antibody (S18; Santa Cruz Biotechnology, Santa Cruz, CA) was added to the extract for overnight incubation; then protein A sepharose (Upstate Biotechnology, Lake Placid, NY) was added for an additional 2 hours.

    Article Title: MRNIP is a replication fork protection factor
    Article Snippet: .. Cell lysis and Western blotting For the preparation of whole-cell extracts, cells were solubilized in lysis buffer [25 mM tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM dithiothreitol (DTT), and 1 mM MgCl2 ] supplemented with benzonase (50 U/μl) (Novagen) and cOmplete protease inhibitors and PhosSTOP phosphatase inhibitors (Roche). .. Gel electrophoresis was performed using 4 to 15% Mini-PROTEAN TGX precast gels (Bio-Rad).

    Article Title: Proteomic analysis links alterations of bioenergetics, mitochondria-ER interactions and proteostasis in hippocampal astrocytes from 3xTg-AD mice
    Article Snippet: .. Final pellets containing mitochondrial-ER enriched fraction (MERE fraction) were lysed with 50 µL of lysis buffer (50 mM TrisHCl (pH 7.4), sodium dodecyl sulphate (SDS) 0.5%, 5 mM EDTA), 5 µL of protease inhibitors cocktail (PIC, Calbiochem). .. After quantification (QuantiPro BCA Assay Kit, QPBSA-1kit, ThermoFisher, Milan, Italy), MERE fractions were snap frozen and stored at -80 °C.

    Transfection:

    Article Title: Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state
    Article Snippet: .. Next day upon transfection, cells were lysed in lysis buffer (1% NP-40, 150 mM NaCl, and 10 mM Tris [pH 7.4]) containing protease inhibitor (Sigma-Aldrich). .. A small fraction of the cell lysate was analyzed by western blotting using rat anti-kindlin-3 antibody (1:1,000 dilution) (clone 181A, generated in-house).

    Protease Inhibitor:

    Article Title: The rare sugar d-tagatose protects plants from downy mildews and is a safe fungicidal agrochemical
    Article Snippet: .. Germinated conidiospores (2 × 106 conidiospores) that had been grown for 24 h in Gamborg B5 medium salt mixture solution (Nihon Pharmaceutical) were ground with a mortar, pestle and zirconia beads (0.5 mm diameter) in 500 μl of extraction buffer (50 mM Tris-HCl buffer, pH 7.5, containing EDTA-free Complete protease inhibitor [Sigma]), then centrifuged at 13,000 rpm for 5 min at 4 °C. .. The supernatant was desalted using 50 mM Tris-HCl buffer (pH 7.5) and an Amicon Ultra-4 (10,000 MWCD; Millipore, Billerica, MA, USA), and the supernatant (=crude enzyme extracts) was mixed with 100 mM of d -tagatose in the reaction mixture (50 mM Tris-HCl pH 7.5, 10 mM MgCl2 and 6 mM ATP).

    Article Title: Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state
    Article Snippet: .. Next day upon transfection, cells were lysed in lysis buffer (1% NP-40, 150 mM NaCl, and 10 mM Tris [pH 7.4]) containing protease inhibitor (Sigma-Aldrich). .. A small fraction of the cell lysate was analyzed by western blotting using rat anti-kindlin-3 antibody (1:1,000 dilution) (clone 181A, generated in-house).

    Article Title: Phase I/II Study of Combination Therapy With Sorafenib, Idarubicin, and Cytarabine in Younger Patients With Acute Myeloid Leukemia
    Article Snippet: .. The cells were washed twice with ice-cold phosphate-buffered saline and then lysed by resuspending them in lysis buffer (20 mmol/L Tris pH 7.4, 100 mmol/L NaCl, 1% Igepal (Sigma, St. Louis, MO), 1 mmol/L ethylenediaminetetra-acetate, 2 mmol/L NaVO4, plus Complete Protease Inhibitor (Roche, Indianapolis, IN) for 30 minutes while rocking. .. Anti-FLT3 antibody (S18; Santa Cruz Biotechnology, Santa Cruz, CA) was added to the extract for overnight incubation; then protein A sepharose (Upstate Biotechnology, Lake Placid, NY) was added for an additional 2 hours.

    Western Blot:

    Article Title: MRNIP is a replication fork protection factor
    Article Snippet: .. Cell lysis and Western blotting For the preparation of whole-cell extracts, cells were solubilized in lysis buffer [25 mM tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM dithiothreitol (DTT), and 1 mM MgCl2 ] supplemented with benzonase (50 U/μl) (Novagen) and cOmplete protease inhibitors and PhosSTOP phosphatase inhibitors (Roche). .. Gel electrophoresis was performed using 4 to 15% Mini-PROTEAN TGX precast gels (Bio-Rad).

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