sodium deoxycholate  (Valiant)

 
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  • 93
    Name:
    Sodium deoxycholate monohydrate
    Description:
    Sodium Deoxycholate Monohydrate
    Catalog Number:
    02199933-cf
    Price:
    10.0
    Category:
    Life Sciences Biochemicals Detergents
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    Structured Review

    Valiant sodium deoxycholate
    Sodium Deoxycholate Monohydrate
    https://www.bioz.com/result/sodium deoxycholate/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate - by Bioz Stars, 2021-03
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Identification and Validation of Novel Spinophilin-associated Proteins in Rodent Striatum Using an Enhanced ex Vivo Shotgun Proteomics Approach *
    Article Snippet: The pellet (P1) was resuspended in 1 ml of isotonic or low ionic strength buffer containing 0.5% Triton X-100 (v/v; Sigma) in a microcentrifuge tube, and samples were then adjusted to a final volume of 2 ml. .. Samples were incubated at 4 °C for 30–60 min and then centrifuged at 9,000 × g for 10 min. Supernatants (S2) were saved for immunoprecipitation, and the P2 pellets were resuspended in 2 ml of isotonic or low ionic strength buffer containing 1% Triton X-100 and 1% sodium deoxycholate (w/v; MP Biomedicals) and sonicated. .. Following incubation at 4 °C for 30 min, samples were then centrifuged at 9,000 × g for 10 min, and the supernatants (S3) were saved for immunoprecipitation.

    Immunoprecipitation:

    Article Title: Identification and Validation of Novel Spinophilin-associated Proteins in Rodent Striatum Using an Enhanced ex Vivo Shotgun Proteomics Approach *
    Article Snippet: The pellet (P1) was resuspended in 1 ml of isotonic or low ionic strength buffer containing 0.5% Triton X-100 (v/v; Sigma) in a microcentrifuge tube, and samples were then adjusted to a final volume of 2 ml. .. Samples were incubated at 4 °C for 30–60 min and then centrifuged at 9,000 × g for 10 min. Supernatants (S2) were saved for immunoprecipitation, and the P2 pellets were resuspended in 2 ml of isotonic or low ionic strength buffer containing 1% Triton X-100 and 1% sodium deoxycholate (w/v; MP Biomedicals) and sonicated. .. Following incubation at 4 °C for 30 min, samples were then centrifuged at 9,000 × g for 10 min, and the supernatants (S3) were saved for immunoprecipitation.

    Sonication:

    Article Title: Identification and Validation of Novel Spinophilin-associated Proteins in Rodent Striatum Using an Enhanced ex Vivo Shotgun Proteomics Approach *
    Article Snippet: The pellet (P1) was resuspended in 1 ml of isotonic or low ionic strength buffer containing 0.5% Triton X-100 (v/v; Sigma) in a microcentrifuge tube, and samples were then adjusted to a final volume of 2 ml. .. Samples were incubated at 4 °C for 30–60 min and then centrifuged at 9,000 × g for 10 min. Supernatants (S2) were saved for immunoprecipitation, and the P2 pellets were resuspended in 2 ml of isotonic or low ionic strength buffer containing 1% Triton X-100 and 1% sodium deoxycholate (w/v; MP Biomedicals) and sonicated. .. Following incubation at 4 °C for 30 min, samples were then centrifuged at 9,000 × g for 10 min, and the supernatants (S3) were saved for immunoprecipitation.

    Lysis:

    Article Title: Perturbation of cytoskeleton dynamics by the opposing effects of Rac1 and Rac1b
    Article Snippet: The Rac activity assay was performed as previously described in reference . .. In brief, HeLa cells were washed twice with cold PBS supplemented with 1 mM MgCl2 and 0.5 mM CaCl2 (Sigma, 223506) and lysed in lysis buffer [50 mM Tris, pH 7.4, 10 mM MgCl2 , 1% Nonidet P-40 (US Biological, N3500), 10% glycerol (Fisher, G33), 100 mM NaCl, 0.5% sodium deoxycholate, 10 mg/ml leupeptin, 1% aprotinin, 10 mg/ml pepstatin (MP Biomedicals, IC19536805), 1 mM phenylmethanesulfonyl fluoride, 10 mg/ml soybean trypsin inhibitor (MP Biomedicals, IC10111325), 10 mM benzamidine (EMD Biosciences, 80057-856)]. .. The lysates were clarified by centrifugation for 5 min at 14,000x g. Cell lysates were normalized for the amount of protein and aliquots were taken to analyze the protein amounts by immunoblotting.

    Article Title: The Proteome of Tetrasphaera elongata is adapted to Changing Conditions in Wastewater Treatment Plants
    Article Snippet: NMR signals were identified and quantified using ChenomX and the known TSP concentration as reference. .. For protein extraction, cells were lysed (in 1% sodium deoxycholate, 50 mM triethylammonium bicarbonate) using the FastPrep-96 Instrument (MP Biomedicals, Eschwege, Germany) for 1 min at 1600 rpm and the All FastDNA-96 (MP Biomedicals) lysis matrix. .. Protein concentrations were assessed by BCA method in triplicates, and approximately 20 µg of protein was subjected to a polyvinylidene fluoride membrane-based proteomic sample preparation [ ].

    Protein Extraction:

    Article Title: The Proteome of Tetrasphaera elongata is adapted to Changing Conditions in Wastewater Treatment Plants
    Article Snippet: NMR signals were identified and quantified using ChenomX and the known TSP concentration as reference. .. For protein extraction, cells were lysed (in 1% sodium deoxycholate, 50 mM triethylammonium bicarbonate) using the FastPrep-96 Instrument (MP Biomedicals, Eschwege, Germany) for 1 min at 1600 rpm and the All FastDNA-96 (MP Biomedicals) lysis matrix. .. Protein concentrations were assessed by BCA method in triplicates, and approximately 20 µg of protein was subjected to a polyvinylidene fluoride membrane-based proteomic sample preparation [ ].

    Protease Inhibitor:

    Article Title: ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer
    Article Snippet: The qRT-PCR primer sequence were listed in Additional file : Table S1. .. Protein extractionCells were homogenized on ice using a homogenizer (LABSONIC® M ultrasonic homogenizer, Sartorius AG, Goettingen, Germany) with 60% amplitude and 0.6 cycle duration for 2 min using 12 mM sodium deoxycholate (Sigma-Aldrich, St Louis, MO, USA), 12 mM sodium lauroyl sarcosine (MP Biomedicals, Santa Ana, CA, USA), 50 mM triethylammonium bicarbonate (Sigma-Aldrich), protease inhibitor cocktail (BioShop, Burlington, Canada), and phosphatase inhibitor cocktail (Bionavas, Toronto, Ontario, Canada). ..

    other:

    Article Title: Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence
    Article Snippet: Cell extracts were recovered in FA-lysis buffer containing 0.1% sodium deoxycholate (SOD) and centrifuged for 15 min at 4°C, 13,000 rpm.

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    Valiant lysis buffer
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