Structured Review

Merck & Co sodium deoxycholate
Sodium Deoxycholate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium deoxycholate/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sodium deoxycholate - by Bioz Stars, 2021-03
86/100 stars

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Electrophoresis:

Article Title: Quality Control of Photosystem II
Article Snippet: The samples subjected to moderate heat or high light treatment were solubilized with 1.5% DM at 4 °C for 10 min, followed by centrifugation at 18,000 × g for 20 min to remove insoluble materials. .. The supernatant was loaded onto a gel with a gradient of 5–13% acrylamide, and electrophoresis was performed for 2 h at 4 °C where the voltage was gradually increased from 70 to 300 V. The composition of the electrode buffers for CN-PAGE was 50 m m Tricine, 7.5 m m imidazole (pH 7.0), 0.05% Triton X-100, and 0.05% deoxycholic acid sodium salt (Merck) for the cathode buffer and 25 m m imidazole HCl (pH 7.0) for the anode buffer. .. The antibodies against the DE loop of the D1 protein, the C terminus of the D1 protein, CP43 and CP47 (the antenna chlorophyll-binding proteins of PSII core showing relative molecular masses of 43,000 and 47,000, respectively, in SDS-PAGE), cyt f , LHCII, and VAR2 (FtsH2 from Arabidopsis ) were used for Western blot analysis.

Clear Native PAGE:

Article Title: Quality Control of Photosystem II
Article Snippet: The samples subjected to moderate heat or high light treatment were solubilized with 1.5% DM at 4 °C for 10 min, followed by centrifugation at 18,000 × g for 20 min to remove insoluble materials. .. The supernatant was loaded onto a gel with a gradient of 5–13% acrylamide, and electrophoresis was performed for 2 h at 4 °C where the voltage was gradually increased from 70 to 300 V. The composition of the electrode buffers for CN-PAGE was 50 m m Tricine, 7.5 m m imidazole (pH 7.0), 0.05% Triton X-100, and 0.05% deoxycholic acid sodium salt (Merck) for the cathode buffer and 25 m m imidazole HCl (pH 7.0) for the anode buffer. .. The antibodies against the DE loop of the D1 protein, the C terminus of the D1 protein, CP43 and CP47 (the antenna chlorophyll-binding proteins of PSII core showing relative molecular masses of 43,000 and 47,000, respectively, in SDS-PAGE), cyt f , LHCII, and VAR2 (FtsH2 from Arabidopsis ) were used for Western blot analysis.

Isolation:

Article Title: Decellularized Matrix from Tumorigenic Human Mesenchymal Stem Cells Promotes Neovascularization with Galectin-1 Dependent Endothelial Interaction
Article Snippet: After 3 days of serum starvation, the monolayer placed on ice was washed in ice cold PBS−− containing EDTA+ Complete® protease inhibitors (Roche). .. The cells were decellularized for 3–5 minutes with 0.25% Triton X (Fluka), 0.25% sodium-deoxycholate (Merck) in PBS−− (Gibco), and the isolated ECM was gently washed in PBS++ (Gibco) with 100 µg/mL RNAse A (Roche) and 10 IU/mL DNAse (Sigma) followed by three washes in PBS++ . .. Morphological changes were observed under phase-contrast illumination using an inverted microscope (Olympus) connected to a digital camera (Olympus, Denmark).

Protease Inhibitor:

Article Title: Glucocorticoid Signaling Enhances Expression of Glucose-Sensing Molecules in Immature Pancreatic Beta-Like Cells Derived from Murine Embryonic Stem Cells In Vitro
Article Snippet: Subsequently, isotype control antibodies or omission of the primary antibodies were used in parallel to the primary antibodies in each staining experiment. .. Cells were lysed on ice in RIPA buffer (Thermo Fisher Scientific) containing 50 mM TRIS, pH 7.4, 150 mM NaCl, 0.1 mM ethylenediaminetetraacetic acid, 1% Triton X-100, 0.5% sodium deoxycholate, and 0.1% sodium dodecyl sulfate (SDS) and supplemented with phosphatase inhibitor cocktail III (Calbiochem/Merck, Bad Soden, Germany) and complete protease inhibitor (Roche, Mannheim, Germany). ..

Article Title: Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-Small Cell Lung Cancer
Article Snippet: Western-Blot Analysis For each analysis, 4 × 106 NCI-H1703, NCI-H522 and IMR-90 cells in the exponential growth phase were taken. .. The extraction of whole cell proteins was made using the RIPA buffer [50 mM Tris HCl; 150 mM NaCl; 0.1% SDS; 1% Igepal (CA-630, Merck, Darmstadt, Germany); 0.5% sodium deoxycholate; protease inhibitor cocktail (Merck); 0.5 mM PMSF]. .. The concentration of proteins was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Impact of gene vector design on the control of recombinant monoclonal antibody production by Chinese hamster ovary cells.
Article Snippet: In this study, we systematically compare two vector design strategies for recombinant monoclonal antibody (Mab) synthesis by Chinese hamster ovary (CHO) cells; a dual open reading frame (ORF) expression vector utilizing separate cytomegalovirus (CMV) promoters to drive heavy chain (HC) and light chain (LC) expression independently, and a single ORF vector design employing a single CMV promoter to drive HC and LC polypeptide expression joined by a foot and mouth disease virus F2A polypeptide self-cleaving linker sequence. .. In this study, we systematically compare two vector design strategies for recombinant monoclonal antibody (Mab) synthesis by Chinese hamster ovary (CHO) cells; a dual open reading frame (ORF) expression vector utilizing separate cytomegalovirus (CMV) promoters to drive heavy chain (HC) and light chain (LC) expression independently, and a single ORF vector design employing a single CMV promoter to drive HC and LC polypeptide expression joined by a foot and mouth disease virus F2A polypeptide self-cleaving linker sequence. .. In this study, we systematically compare two vector design strategies for recombinant monoclonal antibody (Mab) synthesis by Chinese hamster ovary (CHO) cells; a dual open reading frame (ORF) expression vector utilizing separate cytomegalovirus (CMV) promoters to drive heavy chain (HC) and light chain (LC) expression independently, and a single ORF vector design employing a single CMV promoter to drive HC and LC polypeptide expression joined by a foot and mouth disease virus F2A polypeptide self-cleaving linker sequence.

Article Title: Cell line-specific control of recombinant monoclonal antibody production by CHO cells.
Article Snippet: .. In this study we compare the cellular control of recombinant human IgG(4) monoclonal antibody (Mab) synthesis in different CHO cell lines. ..

Lysis:

Article Title: Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells.
Article Snippet: .. Cells were harvested, washed twice with Cell Buffer and once with PBS, and resuspended in ice-cold Lysis Buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% (v/v) Triton X-100, 0.5% (v/v) sodium deoxycholate, 0.1% (w/v) SDS, 1 mMMgCl2, and 100mU/mL benzonase (Merck) containing cOmplete protease inhibitors, 0.1 mL). ..

Incubation:

Article Title: Impact of gene vector design on the control of recombinant monoclonal antibody production by Chinese hamster ovary cells.
Article Snippet: In this study, we systematically compare two vector design strategies for recombinant monoclonal antibody (Mab) synthesis by Chinese hamster ovary (CHO) cells; a dual open reading frame (ORF) expression vector utilizing separate cytomegalovirus (CMV) promoters to drive heavy chain (HC) and light chain (LC) expression independently, and a single ORF vector design employing a single CMV promoter to drive HC and LC polypeptide expression joined by a foot and mouth disease virus F2A polypeptide self-cleaving linker sequence. .. In this study, we systematically compare two vector design strategies for recombinant monoclonal antibody (Mab) synthesis by Chinese hamster ovary (CHO) cells; a dual open reading frame (ORF) expression vector utilizing separate cytomegalovirus (CMV) promoters to drive heavy chain (HC) and light chain (LC) expression independently, and a single ORF vector design employing a single CMV promoter to drive HC and LC polypeptide expression joined by a foot and mouth disease virus F2A polypeptide self-cleaving linker sequence. .. In this study, we systematically compare two vector design strategies for recombinant monoclonal antibody (Mab) synthesis by Chinese hamster ovary (CHO) cells; a dual open reading frame (ORF) expression vector utilizing separate cytomegalovirus (CMV) promoters to drive heavy chain (HC) and light chain (LC) expression independently, and a single ORF vector design employing a single CMV promoter to drive HC and LC polypeptide expression joined by a foot and mouth disease virus F2A polypeptide self-cleaving linker sequence.

Article Title: Cell line-specific control of recombinant monoclonal antibody production by CHO cells.
Article Snippet: .. In this study we compare the cellular control of recombinant human IgG(4) monoclonal antibody (Mab) synthesis in different CHO cell lines. ..

Infection:

Article Title: Characterization of Ribosomal Frameshifting in Theiler's Murine Encephalomyelitis Virus
Article Snippet: Blots were scanned using an Odyssey infrared imaging system (Li-Cor). .. Infected BHK-21 cells from a 25-cm2 flask were washed in cold PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris HCl [pH 8], 150 mM sodium chloride, 1% NP-40 substitute, 0.5% sodium deoxycholate, 0.1% SDS) plus protease inhibitors and Benzonase (Merck) on ice for 20 min. .. Cell debris was removed by centrifugation at 21,000 × g at 4°C for 10 min.

Radio Immunoprecipitation:

Article Title: Characterization of Ribosomal Frameshifting in Theiler's Murine Encephalomyelitis Virus
Article Snippet: Blots were scanned using an Odyssey infrared imaging system (Li-Cor). .. Infected BHK-21 cells from a 25-cm2 flask were washed in cold PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris HCl [pH 8], 150 mM sodium chloride, 1% NP-40 substitute, 0.5% sodium deoxycholate, 0.1% SDS) plus protease inhibitors and Benzonase (Merck) on ice for 20 min. .. Cell debris was removed by centrifugation at 21,000 × g at 4°C for 10 min.

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    Merck & Co xld
    Xld, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xld/product/Merck & Co
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    https://www.bioz.com/result/ripa buffer/product/Merck & Co
    Average 86 stars, based on 1 article reviews
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    Merck & Co sodium deoxycholate
    Sodium Deoxycholate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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