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FUJIFILM sodium deoxycholate
Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
Sodium Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium deoxycholate/product/FUJIFILM
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sodium deoxycholate - by Bioz Stars, 2021-04
86/100 stars

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1) Product Images from "Multifunctional Composite Microcapsules for Oral Delivery of Insulin"

Article Title: Multifunctional Composite Microcapsules for Oral Delivery of Insulin

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms18010054

Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
Figure Legend Snippet: Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.

Techniques Used:

Related Articles

Western Blot:

Article Title: Identification of Licopyranocoumarin and Glycyrurol from Herbal Medicines as Neuroprotective Compounds for Parkinson's Disease
Article Snippet: The active fraction (250.6 mg) was further purified by preparative octadecyl silyl (ODS) HPLC (YMC-Pack ODS-AQ, YMC Co. Ltd., Japan) with 40% aqueous CH3 CN to give pure licopyranocoumarin (10.8 mg) and glycyrurol (4 mg), respectively. .. Western blotting Cells were lysed in RIPA buffer (25 mM HEPES (pH 7.2), 1.5% Triton X-100 (Wako), 1% sodium deoxycholate (Wako), 0.1% SDS, 0.5 M NaCl (Wako), 5 mM EDTA, 50 mM NaF (Sigma), 0.1 mM sodium vanadate (Sigma) and 1 mM phenylmethylsulfonyl fluoride (PMSF) with sonication. ..

Article Title: Preclinical efficacy of Sym004, novel anti-EGFR antibody mixture, in esophageal squamous cell carcinoma cell lines
Article Snippet: Antibodies against EGFR (D38B1), phosphorylated EGFR (Tyr1068 ), Akt, phosphorylated-Akt (Ser473 ), ERK1/2, phosphorylated-ERK1/2 (Thr202 /Thr204 ) and β-actin were purchased from Cell Signaling Technology (Beverly, MA). .. Western blotting analysis Cells were lysed in a radio immunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH8.0), 150-mM sodium chloride, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, and 1% NP-40 (Wako, Osaka, Japan) with protease inhibitor and phosphatase inhibitor (Wako). .. Cell extracts were denatured, subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad, Hercules, CA) using a Trans-Blot Turbo transfer machine (Bio-Rad).

Article Title: Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
Article Snippet: .. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting analysis PEFs_SV40 cells were cultured in 10% FBS DMEM and stimulated with or without 0.1 μg/mL L-LPS for 3 h and 72 h. To obtain total protein extract, cells were lysed in solution which containing 50 mM Tris-HCl (pH 7.4), 0.15 M NaCl, 1% Triton X-100, 2.5 mg/mL sodium deoxycholate (Wako Pure Chemical Industries, Osaka, Japan), and a protease inhibitor cock-tail (1:200 dilution, Nacalai-tesque). .. After calibrating the total protein concentration of cell lysate by a DC Protein assay reagent (Bio-Rad Laboratories, Hercules, CA, USA), 10 μg proteins was subjected to SDS-PAGE (ATTO Corporation, Tokyo, Japan) and blotted onto polyvinylidene fluoride membrane (Immobilon-P; Millipore Corporation, MA, USA).

Sonication:

Article Title: Identification of Licopyranocoumarin and Glycyrurol from Herbal Medicines as Neuroprotective Compounds for Parkinson's Disease
Article Snippet: The active fraction (250.6 mg) was further purified by preparative octadecyl silyl (ODS) HPLC (YMC-Pack ODS-AQ, YMC Co. Ltd., Japan) with 40% aqueous CH3 CN to give pure licopyranocoumarin (10.8 mg) and glycyrurol (4 mg), respectively. .. Western blotting Cells were lysed in RIPA buffer (25 mM HEPES (pH 7.2), 1.5% Triton X-100 (Wako), 1% sodium deoxycholate (Wako), 0.1% SDS, 0.5 M NaCl (Wako), 5 mM EDTA, 50 mM NaF (Sigma), 0.1 mM sodium vanadate (Sigma) and 1 mM phenylmethylsulfonyl fluoride (PMSF) with sonication. ..

Protease Inhibitor:

Article Title: A method of producing genetically manipulated mouse mammary gland
Article Snippet: Adjustment for the variations in the brightness and/or contrast of the entire area of images was performed using Photoshop (Adobe, San Jose, CA, USA). .. Immunoblotting Cells were collected in RIPA buffer (10 mM Tris-HCl (pH 8.0), 1% (w/v) NP40, 0.1% (w/v) sodium deoxycholate (Wako), 0.1% (w/v ) SDS (Wako), 0.15 M NaCl (Wako), 1 mM EDTA, 10 mM NaF (Wako), 1.5 mM Na3 VO4 (Wako), and cOmplete™ Protease Inhibitor Cocktail (Roche)). .. Protein concentrations were titered using the BCA protein assay kit (Thermo Fisher Scientific).

Article Title: Preclinical efficacy of Sym004, novel anti-EGFR antibody mixture, in esophageal squamous cell carcinoma cell lines
Article Snippet: Antibodies against EGFR (D38B1), phosphorylated EGFR (Tyr1068 ), Akt, phosphorylated-Akt (Ser473 ), ERK1/2, phosphorylated-ERK1/2 (Thr202 /Thr204 ) and β-actin were purchased from Cell Signaling Technology (Beverly, MA). .. Western blotting analysis Cells were lysed in a radio immunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH8.0), 150-mM sodium chloride, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, and 1% NP-40 (Wako, Osaka, Japan) with protease inhibitor and phosphatase inhibitor (Wako). .. Cell extracts were denatured, subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad, Hercules, CA) using a Trans-Blot Turbo transfer machine (Bio-Rad).

Article Title: Brexpiprazole, a Serotonin-Dopamine Activity Modulator, Can Sensitize Glioma Stem Cells to Osimertinib, a Third-Generation EGFR-TKI, via Survivin Reduction
Article Snippet: Then, the numbers of PI- and Hoechst-stained cells were counted using a fluorescence microscope (CKX41; Olympus, Tokyo, Japan), and the percentage of PI-stained cells (dead cells) against Hoechst-stained cells (total cells) was calculated [ ]. .. Immunoblot Analysis Cells were washed with ice-cold PBS and lysed in RIPA buffer (10 mM Tris-HCl (pH 7.4), 0.1% SDS, 0.1% sodium deoxycholate, 1% NP-40, 150 mM NaCl, 1 mM EDTA, 1.5 mM Na3 VO4 , 10 mM NaF, 10 mM sodium pyrophosphate, 10 mM sodium β-glycerophosphate. and 1% protease inhibitor cocktail set III (Wako Pure Chemical Industries, Ltd, Osaka, Japan)). .. After centrifugation for 10 minutes at 14,000× g at 4 °C, the supernatants were harvested as the cell lysates and the protein concentration of the cell lysates was measured using the BCA protein assay kit (Thermo Fisher Scientific).

Article Title: Effective RNA Knockdown Using CRISPR-Cas13a and Molecular Targeting of the EML4-ALK Transcript in H3122 Lung Cancer Cells
Article Snippet: Crizotinib (PF-02341066, Selleck Chemicals) (0.25 µM and 2 µM for H3122 and H2228 cells respectively) was used as a positive control. .. Cells were lysed in buffer containing 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid sodium salt, 0.1% SDS, 25 mM Tris-HCL (pH 7.4), and protease inhibitor cocktail set III (Wako Pure Chemical Industries, Japan). .. Proteins were separated by 9% and 11% SDS-PAGE for blotting with ALK and β-actin antibodies, respectively, and then transferred onto a PVDF membrane (Millipore, Bedford, MA, USA).

Article Title: Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
Article Snippet: .. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting analysis PEFs_SV40 cells were cultured in 10% FBS DMEM and stimulated with or without 0.1 μg/mL L-LPS for 3 h and 72 h. To obtain total protein extract, cells were lysed in solution which containing 50 mM Tris-HCl (pH 7.4), 0.15 M NaCl, 1% Triton X-100, 2.5 mg/mL sodium deoxycholate (Wako Pure Chemical Industries, Osaka, Japan), and a protease inhibitor cock-tail (1:200 dilution, Nacalai-tesque). .. After calibrating the total protein concentration of cell lysate by a DC Protein assay reagent (Bio-Rad Laboratories, Hercules, CA, USA), 10 μg proteins was subjected to SDS-PAGE (ATTO Corporation, Tokyo, Japan) and blotted onto polyvinylidene fluoride membrane (Immobilon-P; Millipore Corporation, MA, USA).

Radio Immunoprecipitation:

Article Title: Preclinical efficacy of Sym004, novel anti-EGFR antibody mixture, in esophageal squamous cell carcinoma cell lines
Article Snippet: Antibodies against EGFR (D38B1), phosphorylated EGFR (Tyr1068 ), Akt, phosphorylated-Akt (Ser473 ), ERK1/2, phosphorylated-ERK1/2 (Thr202 /Thr204 ) and β-actin were purchased from Cell Signaling Technology (Beverly, MA). .. Western blotting analysis Cells were lysed in a radio immunoprecipitation assay (RIPA) buffer containing 50 mM Tris-HCl (pH8.0), 150-mM sodium chloride, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, and 1% NP-40 (Wako, Osaka, Japan) with protease inhibitor and phosphatase inhibitor (Wako). .. Cell extracts were denatured, subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad, Hercules, CA) using a Trans-Blot Turbo transfer machine (Bio-Rad).

Lysis:

Article Title: Nasopharyngeal Haemophilus influenzae Carriage in Japanese Children Attending Day-Care Centers ▿
Article Snippet: The pellet was mixed with an equal volume of melted 1.2% chromosomal-grade agarose (Bio-Rad Laboratories, Richmond, CA). .. The mixture was poured in a mold and chilled, and the resultant plugs were treated with 0.5 ml of lysis solution I (6 mM Tris-HCl [pH 8.0], 0.1 M EDTA, 0.5% Brij 58, 0.2% sodium deoxycholate, 0.5% Sarkosyl, 0.8 mg/ml of lysozyme [Wako Pure Chemical Industries, Ltd., Japan], and 10 μg/ml of RNase [Nippon Gene, Co., Ltd., Tokyo, Japan]) at 37°C for 12 h and treated with 0.5 ml of lysis solution II (0.5 M EDTA [pH 8.0], 1% Sarkosyl, and 0.25 mg/ml of proteinase K [Wako Pure Chemical Industries, Ltd.]) at 50°C for 24 h. For restriction endonuclease digestion, the plugs were incubated with 30 U of SmaI (Takara Bio Inc.) in restriction enzyme buffer at 30°C for 16 h. DNA fragments were electrophoresed in a 1% agarose gel at 6 V/cm and 14°C for 22 h with a 3- to 60-s pulse time with CHEF Mapper and CHEF Mapper XA pulsed-field electrophoresis systems (Bio-Rad Laboratories). .. A bacteriophage lambda ladder (Bio-Rad Laboratories) was used as a size standard.

Incubation:

Article Title: Nasopharyngeal Haemophilus influenzae Carriage in Japanese Children Attending Day-Care Centers ▿
Article Snippet: The pellet was mixed with an equal volume of melted 1.2% chromosomal-grade agarose (Bio-Rad Laboratories, Richmond, CA). .. The mixture was poured in a mold and chilled, and the resultant plugs were treated with 0.5 ml of lysis solution I (6 mM Tris-HCl [pH 8.0], 0.1 M EDTA, 0.5% Brij 58, 0.2% sodium deoxycholate, 0.5% Sarkosyl, 0.8 mg/ml of lysozyme [Wako Pure Chemical Industries, Ltd., Japan], and 10 μg/ml of RNase [Nippon Gene, Co., Ltd., Tokyo, Japan]) at 37°C for 12 h and treated with 0.5 ml of lysis solution II (0.5 M EDTA [pH 8.0], 1% Sarkosyl, and 0.25 mg/ml of proteinase K [Wako Pure Chemical Industries, Ltd.]) at 50°C for 24 h. For restriction endonuclease digestion, the plugs were incubated with 30 U of SmaI (Takara Bio Inc.) in restriction enzyme buffer at 30°C for 16 h. DNA fragments were electrophoresed in a 1% agarose gel at 6 V/cm and 14°C for 22 h with a 3- to 60-s pulse time with CHEF Mapper and CHEF Mapper XA pulsed-field electrophoresis systems (Bio-Rad Laboratories). .. A bacteriophage lambda ladder (Bio-Rad Laboratories) was used as a size standard.

Agarose Gel Electrophoresis:

Article Title: Nasopharyngeal Haemophilus influenzae Carriage in Japanese Children Attending Day-Care Centers ▿
Article Snippet: The pellet was mixed with an equal volume of melted 1.2% chromosomal-grade agarose (Bio-Rad Laboratories, Richmond, CA). .. The mixture was poured in a mold and chilled, and the resultant plugs were treated with 0.5 ml of lysis solution I (6 mM Tris-HCl [pH 8.0], 0.1 M EDTA, 0.5% Brij 58, 0.2% sodium deoxycholate, 0.5% Sarkosyl, 0.8 mg/ml of lysozyme [Wako Pure Chemical Industries, Ltd., Japan], and 10 μg/ml of RNase [Nippon Gene, Co., Ltd., Tokyo, Japan]) at 37°C for 12 h and treated with 0.5 ml of lysis solution II (0.5 M EDTA [pH 8.0], 1% Sarkosyl, and 0.25 mg/ml of proteinase K [Wako Pure Chemical Industries, Ltd.]) at 50°C for 24 h. For restriction endonuclease digestion, the plugs were incubated with 30 U of SmaI (Takara Bio Inc.) in restriction enzyme buffer at 30°C for 16 h. DNA fragments were electrophoresed in a 1% agarose gel at 6 V/cm and 14°C for 22 h with a 3- to 60-s pulse time with CHEF Mapper and CHEF Mapper XA pulsed-field electrophoresis systems (Bio-Rad Laboratories). .. A bacteriophage lambda ladder (Bio-Rad Laboratories) was used as a size standard.

Electrophoresis:

Article Title: Nasopharyngeal Haemophilus influenzae Carriage in Japanese Children Attending Day-Care Centers ▿
Article Snippet: The pellet was mixed with an equal volume of melted 1.2% chromosomal-grade agarose (Bio-Rad Laboratories, Richmond, CA). .. The mixture was poured in a mold and chilled, and the resultant plugs were treated with 0.5 ml of lysis solution I (6 mM Tris-HCl [pH 8.0], 0.1 M EDTA, 0.5% Brij 58, 0.2% sodium deoxycholate, 0.5% Sarkosyl, 0.8 mg/ml of lysozyme [Wako Pure Chemical Industries, Ltd., Japan], and 10 μg/ml of RNase [Nippon Gene, Co., Ltd., Tokyo, Japan]) at 37°C for 12 h and treated with 0.5 ml of lysis solution II (0.5 M EDTA [pH 8.0], 1% Sarkosyl, and 0.25 mg/ml of proteinase K [Wako Pure Chemical Industries, Ltd.]) at 50°C for 24 h. For restriction endonuclease digestion, the plugs were incubated with 30 U of SmaI (Takara Bio Inc.) in restriction enzyme buffer at 30°C for 16 h. DNA fragments were electrophoresed in a 1% agarose gel at 6 V/cm and 14°C for 22 h with a 3- to 60-s pulse time with CHEF Mapper and CHEF Mapper XA pulsed-field electrophoresis systems (Bio-Rad Laboratories). .. A bacteriophage lambda ladder (Bio-Rad Laboratories) was used as a size standard.

Nucleic Acid Electrophoresis:

Article Title: Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
Article Snippet: .. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting analysis PEFs_SV40 cells were cultured in 10% FBS DMEM and stimulated with or without 0.1 μg/mL L-LPS for 3 h and 72 h. To obtain total protein extract, cells were lysed in solution which containing 50 mM Tris-HCl (pH 7.4), 0.15 M NaCl, 1% Triton X-100, 2.5 mg/mL sodium deoxycholate (Wako Pure Chemical Industries, Osaka, Japan), and a protease inhibitor cock-tail (1:200 dilution, Nacalai-tesque). .. After calibrating the total protein concentration of cell lysate by a DC Protein assay reagent (Bio-Rad Laboratories, Hercules, CA, USA), 10 μg proteins was subjected to SDS-PAGE (ATTO Corporation, Tokyo, Japan) and blotted onto polyvinylidene fluoride membrane (Immobilon-P; Millipore Corporation, MA, USA).

Cell Culture:

Article Title: Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
Article Snippet: .. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting analysis PEFs_SV40 cells were cultured in 10% FBS DMEM and stimulated with or without 0.1 μg/mL L-LPS for 3 h and 72 h. To obtain total protein extract, cells were lysed in solution which containing 50 mM Tris-HCl (pH 7.4), 0.15 M NaCl, 1% Triton X-100, 2.5 mg/mL sodium deoxycholate (Wako Pure Chemical Industries, Osaka, Japan), and a protease inhibitor cock-tail (1:200 dilution, Nacalai-tesque). .. After calibrating the total protein concentration of cell lysate by a DC Protein assay reagent (Bio-Rad Laboratories, Hercules, CA, USA), 10 μg proteins was subjected to SDS-PAGE (ATTO Corporation, Tokyo, Japan) and blotted onto polyvinylidene fluoride membrane (Immobilon-P; Millipore Corporation, MA, USA).

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  • 99
    FUJIFILM sodium deoxycholate
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Sodium Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate/product/FUJIFILM
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    86
    FUJIFILM sodium deoxycholate solution
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Sodium Deoxycholate Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate solution/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate solution - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    FUJIFILM deoxycholate
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholate - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.

    Journal: International Journal of Molecular Sciences

    Article Title: Multifunctional Composite Microcapsules for Oral Delivery of Insulin

    doi: 10.3390/ijms18010054

    Figure Lengend Snippet: Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.

    Article Snippet: Sodium deoxycholate and poly(lactic-co -glycolic acid) (PLGA 75/25, molecular weight of 20 kDa) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).

    Techniques: