sodium bisulfite treated dna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sodium bisulfite treated dna
    Methylation levels assessed by <t>RT-MSP</t> for CGIs located on CCDC17 , PVR , and MAP3K11 gene bodies in SCC, KA, and normal epidermis. (a) The methylation level of each sample in SCC cell lines, clinical SCC samples, clinical KA samples, clinical normal epidermis samples, and controls including methylated <t>DNA</t> (M) and unmethylated DNA (U) is indicated as a bar graph. The vertical and horizontal axes indicate the methylation level (%) and sample ID, respectively. (b) Averages and standard deviations of methylation levels in each sample group are indicated as bar graphs. The vertical and horizontal axes indicate the average methylation level (%) with standard deviation and sample ID, respectively. An asterisk represents a significant difference ( P
    Sodium Bisulfite Treated Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium bisulfite treated dna/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium bisulfite treated dna - by Bioz Stars, 2020-07
    89/100 stars

    Images

    1) Product Images from "Aberrant DNA Methylation in Keratoacanthoma"

    Article Title: Aberrant DNA Methylation in Keratoacanthoma

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0165370

    Methylation levels assessed by RT-MSP for CGIs located on CCDC17 , PVR , and MAP3K11 gene bodies in SCC, KA, and normal epidermis. (a) The methylation level of each sample in SCC cell lines, clinical SCC samples, clinical KA samples, clinical normal epidermis samples, and controls including methylated DNA (M) and unmethylated DNA (U) is indicated as a bar graph. The vertical and horizontal axes indicate the methylation level (%) and sample ID, respectively. (b) Averages and standard deviations of methylation levels in each sample group are indicated as bar graphs. The vertical and horizontal axes indicate the average methylation level (%) with standard deviation and sample ID, respectively. An asterisk represents a significant difference ( P
    Figure Legend Snippet: Methylation levels assessed by RT-MSP for CGIs located on CCDC17 , PVR , and MAP3K11 gene bodies in SCC, KA, and normal epidermis. (a) The methylation level of each sample in SCC cell lines, clinical SCC samples, clinical KA samples, clinical normal epidermis samples, and controls including methylated DNA (M) and unmethylated DNA (U) is indicated as a bar graph. The vertical and horizontal axes indicate the methylation level (%) and sample ID, respectively. (b) Averages and standard deviations of methylation levels in each sample group are indicated as bar graphs. The vertical and horizontal axes indicate the average methylation level (%) with standard deviation and sample ID, respectively. An asterisk represents a significant difference ( P

    Techniques Used: Methylation, Standard Deviation

    Bisulfite sequencing analysis in CGIs located on CCDC17 , PVR , and MAP3K11 gene bodies. The results of bisulfite sequencing of the CGIs in representative samples in each sample group are shown. Horizontal rows indicate each CGI located on the CCDC17 , PVR , or MAP3K11 gene body. The vertical rows indicate each representative sample of SCC, KA, or normal epidermis. The sample ID is the number in the upper left corner of each figure. Closed and open circles indicate methylated and unmethylated CpG sites, respectively. Closed and open triangles indicate the location of the RT-MSP primer sets specific to the methylated and unmethylated DNA sequences, respectively. The vertical and horizontal number rows indicate each clone and CpG site, respectively.
    Figure Legend Snippet: Bisulfite sequencing analysis in CGIs located on CCDC17 , PVR , and MAP3K11 gene bodies. The results of bisulfite sequencing of the CGIs in representative samples in each sample group are shown. Horizontal rows indicate each CGI located on the CCDC17 , PVR , or MAP3K11 gene body. The vertical rows indicate each representative sample of SCC, KA, or normal epidermis. The sample ID is the number in the upper left corner of each figure. Closed and open circles indicate methylated and unmethylated CpG sites, respectively. Closed and open triangles indicate the location of the RT-MSP primer sets specific to the methylated and unmethylated DNA sequences, respectively. The vertical and horizontal number rows indicate each clone and CpG site, respectively.

    Techniques Used: Methylation Sequencing, Methylation

    2) Product Images from "Silencing of interferon regulatory factor gene 6 in melanoma"

    Article Title: Silencing of interferon regulatory factor gene 6 in melanoma

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0184444

    Methylation levels of the CGI located in the 5' region of IRF6 in 21 clinical melanoma samples and 24 clinical melanocytic nevus samples. Each methylation level was determined using RT-MSP. The vertical and horizontal axes indicate the methylation level (%) and sample ID, respectively. M and U indicate controls of methylated and unmethylated DNA, respectively.
    Figure Legend Snippet: Methylation levels of the CGI located in the 5' region of IRF6 in 21 clinical melanoma samples and 24 clinical melanocytic nevus samples. Each methylation level was determined using RT-MSP. The vertical and horizontal axes indicate the methylation level (%) and sample ID, respectively. M and U indicate controls of methylated and unmethylated DNA, respectively.

    Techniques Used: Methylation

    Bisulfite sequencing analysis to confirm the RT-MSP data. The results of bisulfite sequencing of the 5' IRF6 CGI for representative samples are shown. The clinical melanoma samples #10 and #17 were examined as representatives of samples with high methylation levels. Normal cultured melanocytes MC2 and clinical melanocytic nevus samples #23 and #34 were examined as representatives of samples with low methylation levels. Closed and open circles indicate methylated and unmethylated CpG sites, respectively. Closed and open triangles indicate the location of the RT-MSP primer sets specific to the methylated and unmethylated DNA sequences, respectively. The vertical and horizontal numbered rows indicate each clone and CpG site, respectively.
    Figure Legend Snippet: Bisulfite sequencing analysis to confirm the RT-MSP data. The results of bisulfite sequencing of the 5' IRF6 CGI for representative samples are shown. The clinical melanoma samples #10 and #17 were examined as representatives of samples with high methylation levels. Normal cultured melanocytes MC2 and clinical melanocytic nevus samples #23 and #34 were examined as representatives of samples with low methylation levels. Closed and open circles indicate methylated and unmethylated CpG sites, respectively. Closed and open triangles indicate the location of the RT-MSP primer sets specific to the methylated and unmethylated DNA sequences, respectively. The vertical and horizontal numbered rows indicate each clone and CpG site, respectively.

    Techniques Used: Methylation Sequencing, Methylation, Cell Culture

    3) Product Images from "VILIP-1 Downregulation in Non-Small Cell Lung Carcinomas: Mechanisms and Prediction of Survival"

    Article Title: VILIP-1 Downregulation in Non-Small Cell Lung Carcinomas: Mechanisms and Prediction of Survival

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0001698

    Methylation status of VILIP-1 promoter in NSCLC cell lines. A. Schematic map of VILIP-1 2kb promoter and CpG islands around exon1. Filled boxes, CpG islands. Open boxes, exons. Vertical ticks, CpG sites on the expanded axis. Start of exon 1 is marked at +1. TSS, translation start site (ATG start codon). B. MSP analysis of cell lines. Bands in lanes M are methylated, bands in lanes U are unmethylated. H 2 O: water was added instead of DNA; Untreated DNA: genomic DNA without treatment with sodium bisulfite; NHBE 1 and NHBE 2: DNA from two different individuals. C. Representative results of bisulfite sequencing of the second CpG island of VILIP-1 promoter in VILIP-1-expressing cell lines (+) and VILIP-1-nonexpressing cell lines (−). Open and filled squares indicate unmethylated and methylated CpG sites, respectively. Six to eight clones of PCR products amplified from bisulfite-treated genomic DNA were sequenced for each cell line. D. Reactivation of VILIP-1 expression by 5′-Aza-dC treatment in cell lines. VILIP-1 protein expression was determined by immunoblot analysis. GAPDH was included as a control for equal loading.
    Figure Legend Snippet: Methylation status of VILIP-1 promoter in NSCLC cell lines. A. Schematic map of VILIP-1 2kb promoter and CpG islands around exon1. Filled boxes, CpG islands. Open boxes, exons. Vertical ticks, CpG sites on the expanded axis. Start of exon 1 is marked at +1. TSS, translation start site (ATG start codon). B. MSP analysis of cell lines. Bands in lanes M are methylated, bands in lanes U are unmethylated. H 2 O: water was added instead of DNA; Untreated DNA: genomic DNA without treatment with sodium bisulfite; NHBE 1 and NHBE 2: DNA from two different individuals. C. Representative results of bisulfite sequencing of the second CpG island of VILIP-1 promoter in VILIP-1-expressing cell lines (+) and VILIP-1-nonexpressing cell lines (−). Open and filled squares indicate unmethylated and methylated CpG sites, respectively. Six to eight clones of PCR products amplified from bisulfite-treated genomic DNA were sequenced for each cell line. D. Reactivation of VILIP-1 expression by 5′-Aza-dC treatment in cell lines. VILIP-1 protein expression was determined by immunoblot analysis. GAPDH was included as a control for equal loading.

    Techniques Used: Methylation, Methylation Sequencing, Expressing, Clone Assay, Polymerase Chain Reaction, Amplification

    Related Articles

    Clone Assay:

    Article Title: Parallel assessment of CpG methylation by two-color hybridization with oligonucleotide arrays
    Article Snippet: .. The 190-bp amplicon of sodium bisulfite-treated DNA was cloned into plasmid pCR 2.1 using a TA cloning kit (Invitrogen, Carlsbad, CA) and vendor protocols. .. Plasmid was isolated from 18 individual colonies and the insert sequenced using an ABI3100 sequencer with T7 and M13 primers and dye-terminated DNA sequencing protocols.

    Amplification:

    Article Title: Parallel assessment of CpG methylation by two-color hybridization with oligonucleotide arrays
    Article Snippet: .. The 190-bp amplicon of sodium bisulfite-treated DNA was cloned into plasmid pCR 2.1 using a TA cloning kit (Invitrogen, Carlsbad, CA) and vendor protocols. .. Plasmid was isolated from 18 individual colonies and the insert sequenced using an ABI3100 sequencer with T7 and M13 primers and dye-terminated DNA sequencing protocols.

    Article Title: Murine De Novo Methyltransferase Dnmt3a Demonstrates Strand Asymmetry and Site Preference in the Methylation of DNA In Vitro
    Article Snippet: .. Top and bottom strands of the sodium bisulfite-treated DNA were amplified and ligated into the TOPO TA cloning vector (Invitrogen). .. Both strands of each clone were sequenced using the EXCEL II sequencing kit (Epicentre) and analyzed on the Li-Cor Sequencer (model 4200L).

    Article Title: Aberrant DNA Methylation in Keratoacanthoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) by using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). ..

    Article Title: Silencing of interferon regulatory factor gene 6 in melanoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). .. The sequences of the M set primers were: 5'-GTAGGGTGGGACGTTGGACGGAC-3' for the forward primer and 5'-TAACCACGCCCCCCGACGTTCG-3' for the reverse primer, which were designed to amplify from ‒127 bp to ‒22 bp of the IRF6 gene based on the major IRF6 start site (based on the IRF6 sequence in NC_000001.11).

    Methylation:

    Article Title: Aberrant DNA Methylation in Keratoacanthoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) by using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). ..

    Article Title: Silencing of interferon regulatory factor gene 6 in melanoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). .. The sequences of the M set primers were: 5'-GTAGGGTGGGACGTTGGACGGAC-3' for the forward primer and 5'-TAACCACGCCCCCCGACGTTCG-3' for the reverse primer, which were designed to amplify from ‒127 bp to ‒22 bp of the IRF6 gene based on the major IRF6 start site (based on the IRF6 sequence in NC_000001.11).

    TA Cloning:

    Article Title: Parallel assessment of CpG methylation by two-color hybridization with oligonucleotide arrays
    Article Snippet: .. The 190-bp amplicon of sodium bisulfite-treated DNA was cloned into plasmid pCR 2.1 using a TA cloning kit (Invitrogen, Carlsbad, CA) and vendor protocols. .. Plasmid was isolated from 18 individual colonies and the insert sequenced using an ABI3100 sequencer with T7 and M13 primers and dye-terminated DNA sequencing protocols.

    Article Title: Murine De Novo Methyltransferase Dnmt3a Demonstrates Strand Asymmetry and Site Preference in the Methylation of DNA In Vitro
    Article Snippet: .. Top and bottom strands of the sodium bisulfite-treated DNA were amplified and ligated into the TOPO TA cloning vector (Invitrogen). .. Both strands of each clone were sequenced using the EXCEL II sequencing kit (Epicentre) and analyzed on the Li-Cor Sequencer (model 4200L).

    Spectrophotometry:

    Article Title: DNA Methylation Profiles of the Brain-Derived Neurotrophic Factor (BDNF) Gene as a Potent Diagnostic Biomarker in Major Depression
    Article Snippet: .. The concentration of sodium bisulfite-treated DNA was measured using an ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA), and 10 ng of treated DNA was applied in a region-specific PCR. .. The PCR reactions were carried out in a total volume of 5 µL using 1 µmol of each primer, 200 µM dNTP, 0.2 U HotStar Taq DNA polymerase (Qiagen), 15 mM MgCl2, and 10× PCR buffer (final concentration 1×).

    SYBR Green Assay:

    Article Title: Aberrant DNA Methylation in Keratoacanthoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) by using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). ..

    Article Title: Silencing of interferon regulatory factor gene 6 in melanoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). .. The sequences of the M set primers were: 5'-GTAGGGTGGGACGTTGGACGGAC-3' for the forward primer and 5'-TAACCACGCCCCCCGACGTTCG-3' for the reverse primer, which were designed to amplify from ‒127 bp to ‒22 bp of the IRF6 gene based on the major IRF6 start site (based on the IRF6 sequence in NC_000001.11).

    Concentration Assay:

    Article Title: DNA Methylation Profiles of the Brain-Derived Neurotrophic Factor (BDNF) Gene as a Potent Diagnostic Biomarker in Major Depression
    Article Snippet: .. The concentration of sodium bisulfite-treated DNA was measured using an ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA), and 10 ng of treated DNA was applied in a region-specific PCR. .. The PCR reactions were carried out in a total volume of 5 µL using 1 µmol of each primer, 200 µM dNTP, 0.2 U HotStar Taq DNA polymerase (Qiagen), 15 mM MgCl2, and 10× PCR buffer (final concentration 1×).

    Polymerase Chain Reaction:

    Article Title: Parallel assessment of CpG methylation by two-color hybridization with oligonucleotide arrays
    Article Snippet: .. The 190-bp amplicon of sodium bisulfite-treated DNA was cloned into plasmid pCR 2.1 using a TA cloning kit (Invitrogen, Carlsbad, CA) and vendor protocols. .. Plasmid was isolated from 18 individual colonies and the insert sequenced using an ABI3100 sequencer with T7 and M13 primers and dye-terminated DNA sequencing protocols.

    Article Title: Expression Analysis of Genes Involved in the RB/E2F Pathway in Astrocytic Tumors
    Article Snippet: .. Each PCR was performed in a final reaction volume of 25 μL containing 2 μL of sodium bisulfite-treated DNA; 2.5 μL of 10X reaction buffer; 1 μL of MgCl2 (1.5 mM); 0.5 μL of each dNTP (10 mM) (Life Technologies); 1 μL of each primer (10 mM); 0.3 μL of Taq DNA polymerase (Promega, Inc.); and ultrapure H2 O to complete the final reaction volume. .. The PCR amplification products were analyzed on 3% agarose gels stained with ethidium bromide and subsequently purified using the EZ-10 Spin Column PCR Product Purification kit (Bio Basic/Ludwig Biotec), following the manufacturer's instructions.

    Article Title: Aberrant DNA Methylation in Keratoacanthoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) by using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). ..

    Article Title: Silencing of interferon regulatory factor gene 6 in melanoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). .. The sequences of the M set primers were: 5'-GTAGGGTGGGACGTTGGACGGAC-3' for the forward primer and 5'-TAACCACGCCCCCCGACGTTCG-3' for the reverse primer, which were designed to amplify from ‒127 bp to ‒22 bp of the IRF6 gene based on the major IRF6 start site (based on the IRF6 sequence in NC_000001.11).

    Article Title: Enzymatic Regional Methylation Assay: A Novel Method to Quantify Regional CpG Methylation Density
    Article Snippet: .. By use of 100 ng of resuspended, sodium bisulfite-treated DNA, PCR was performed in a 50-μL reaction containing 400 nM of each primer (GIBCO BRL), 1× PCR buffer (16.6 mM ammonium sulfate/67 mM Tris at pH 8.8/6.7 mM MgCl2 /10 mM 2-mercaptoethanol) and 1.25 mM of each dNTP (Pharmacia). .. Reactions were hot started at 95°C for 5 min and held at 80°C before addition of 1.25 U of Taq polymerase (Sigma).

    Article Title: VILIP-1 Downregulation in Non-Small Cell Lung Carcinomas: Mechanisms and Prediction of Survival
    Article Snippet: .. MSP was performed as follows: PCR reactions comprised 2 µl of sodium bisulfite treated DNA, 0.2 mM of dNTPs, 0.2 µM of forward and reverse primers each, 1× reaction buffer, 0.2 mM of MgCl2 and 5 units of Ampli Taq Gold DNA polymerase (Applied Biosystems, Foster city, CA). .. Methylation and non-methylation specific primers ( ) were used to uncover the methylation status of sodium bisulfite modified DNA.

    Article Title: DNA Methylation Profiles of the Brain-Derived Neurotrophic Factor (BDNF) Gene as a Potent Diagnostic Biomarker in Major Depression
    Article Snippet: .. The concentration of sodium bisulfite-treated DNA was measured using an ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE, USA), and 10 ng of treated DNA was applied in a region-specific PCR. .. The PCR reactions were carried out in a total volume of 5 µL using 1 µmol of each primer, 200 µM dNTP, 0.2 U HotStar Taq DNA polymerase (Qiagen), 15 mM MgCl2, and 10× PCR buffer (final concentration 1×).

    Sequencing:

    Article Title: Aberrant DNA Methylation in Keratoacanthoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) by using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). ..

    Article Title: Silencing of interferon regulatory factor gene 6 in melanoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). .. The sequences of the M set primers were: 5'-GTAGGGTGGGACGTTGGACGGAC-3' for the forward primer and 5'-TAACCACGCCCCCCGACGTTCG-3' for the reverse primer, which were designed to amplify from ‒127 bp to ‒22 bp of the IRF6 gene based on the major IRF6 start site (based on the IRF6 sequence in NC_000001.11).

    Real-time Polymerase Chain Reaction:

    Article Title: Aberrant DNA Methylation in Keratoacanthoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) by using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). ..

    Article Title: Silencing of interferon regulatory factor gene 6 in melanoma
    Article Snippet: .. For RT-MSP, 1.0 μl of the sodium bisulfite-treated DNA was amplified with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using a mixture of primer sets that were specific to the methylated or unmethylated DNA sequence (M or U set, respectively) and the SYBR Green PCR Master Mix I (Toyobo, Osaka, Japan). .. The sequences of the M set primers were: 5'-GTAGGGTGGGACGTTGGACGGAC-3' for the forward primer and 5'-TAACCACGCCCCCCGACGTTCG-3' for the reverse primer, which were designed to amplify from ‒127 bp to ‒22 bp of the IRF6 gene based on the major IRF6 start site (based on the IRF6 sequence in NC_000001.11).

    Plasmid Preparation:

    Article Title: Parallel assessment of CpG methylation by two-color hybridization with oligonucleotide arrays
    Article Snippet: .. The 190-bp amplicon of sodium bisulfite-treated DNA was cloned into plasmid pCR 2.1 using a TA cloning kit (Invitrogen, Carlsbad, CA) and vendor protocols. .. Plasmid was isolated from 18 individual colonies and the insert sequenced using an ABI3100 sequencer with T7 and M13 primers and dye-terminated DNA sequencing protocols.

    Article Title: Murine De Novo Methyltransferase Dnmt3a Demonstrates Strand Asymmetry and Site Preference in the Methylation of DNA In Vitro
    Article Snippet: .. Top and bottom strands of the sodium bisulfite-treated DNA were amplified and ligated into the TOPO TA cloning vector (Invitrogen). .. Both strands of each clone were sequenced using the EXCEL II sequencing kit (Epicentre) and analyzed on the Li-Cor Sequencer (model 4200L).

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    Thermo Fisher sodium bisulfite
    Sodium Bisulfite, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium bisulfite/product/Thermo Fisher
    Average 93 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    sodium bisulfite - by Bioz Stars, 2020-07
    93/100 stars
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