sodium bisulfite treated dna  (Millipore)


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    Name:
    Sodium bisulfite
    Description:

    Catalog Number:
    28-1970
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    Structured Review

    Millipore sodium bisulfite treated dna
    Sodium bisulfite

    https://www.bioz.com/result/sodium bisulfite treated dna/product/Millipore
    Average 97 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    sodium bisulfite treated dna - by Bioz Stars, 2020-07
    97/100 stars

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    Related Articles

    Chromatography:

    Article Title: 5-methylcytosine promotes mRNA export — NSUN2 as the methyltransferase and ALYREF as an m5C reader
    Article Snippet: .. The RNA pellet was resuspended in 100 μl bisulfite solution (pH 5.1), which is a 100:1 mixture of 40% sodium bisulfite (Sigma) and 600 μM hydroquinone (Sigma) and subjected to heat incubation at 75 °C for 4 h. The reaction mixture was desalted by twice passing it through Micro Bio-spin 6 chromatography columns (Bio-Rad) and then desulfonated by incubation with an equal volume of 1 M Tris (pH 9.0) at 75 °C for 1 h. After ethanol precipitation, the RNAs were resuspended in 10 μl of RNase-free water and used for library construction or validation. .. Sanger sequencing of PCR products For the validation of methylated sites, a 300 ng aliquot of sodium bisulfite converted RNA was reverse transcribed into cDNA using ACT random hexamers and Superscript III Reverse Transcriptase Kit (Invitrogen) according to the manufacturer's instructions. cDNA was amplified by PCR using normal primers for untreated mRNAs and specific primers for bisulfite-treated mRNAs.

    Methylation:

    Article Title: miR-31 and its host gene lncRNA LOC554202 are regulated by promoter hypermethylation in triple-negative breast cancer
    Article Snippet: .. Preparation of bisulfite-modified DNA for methylation analysis Genomic DNA (1-2 μg) was denatured in 0.3 M NaOH for 30 min at 42°C, and then the unmethylated cytosine residues were sulphonated by incubation in 3.12 M sodium bisulfite (pH 5.0; Sigma)/5 mM hydroquinone (Sigma, MO, USA) at 55°C for 16 h. The sulphonated DNA was recovered using the QIAquick Gel Extraction system (Qiagen, MA, USA), according to the manufacturer's recommendations. ..

    Article Title: MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients
    Article Snippet: .. Methylation-specific PCR for TP53 and MGMT promoter methylation analysis Sodium bisulfite modification of isolated genomic DNA was performed using the CpGenome DNA kit (Chemicon International Inc., Temecula, CA, USA) according to the manufacturer’s protocol. ..

    Ethanol Precipitation:

    Article Title: 5-methylcytosine promotes mRNA export — NSUN2 as the methyltransferase and ALYREF as an m5C reader
    Article Snippet: .. The RNA pellet was resuspended in 100 μl bisulfite solution (pH 5.1), which is a 100:1 mixture of 40% sodium bisulfite (Sigma) and 600 μM hydroquinone (Sigma) and subjected to heat incubation at 75 °C for 4 h. The reaction mixture was desalted by twice passing it through Micro Bio-spin 6 chromatography columns (Bio-Rad) and then desulfonated by incubation with an equal volume of 1 M Tris (pH 9.0) at 75 °C for 1 h. After ethanol precipitation, the RNAs were resuspended in 10 μl of RNase-free water and used for library construction or validation. .. Sanger sequencing of PCR products For the validation of methylated sites, a 300 ng aliquot of sodium bisulfite converted RNA was reverse transcribed into cDNA using ACT random hexamers and Superscript III Reverse Transcriptase Kit (Invitrogen) according to the manufacturer's instructions. cDNA was amplified by PCR using normal primers for untreated mRNAs and specific primers for bisulfite-treated mRNAs.

    Purification:

    Article Title: Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors
    Article Snippet: .. DNA was denatured by 0.3 M NaOH at 42°C for 30 min. Then 3 M sodium bisulfite (Sigma) and 10 mmol/L hydroquinone were added, which was further incubated at 50°C for 16 h. Modified DNA was purified using Wizard DNA purification resin (Promega), re-treated with 0.3 M NaOH at 37°C for 15 min, precipitated with ethanol, and resuspended in 50 μL water. ..

    Modification:

    Article Title: Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors
    Article Snippet: .. DNA was denatured by 0.3 M NaOH at 42°C for 30 min. Then 3 M sodium bisulfite (Sigma) and 10 mmol/L hydroquinone were added, which was further incubated at 50°C for 16 h. Modified DNA was purified using Wizard DNA purification resin (Promega), re-treated with 0.3 M NaOH at 37°C for 15 min, precipitated with ethanol, and resuspended in 50 μL water. ..

    Article Title: DNMT1 mediates chemosensitivity by reducing methylation of miRNA-20a promoter in glioma cells
    Article Snippet: .. Then, the genomic DNA of each sample was treated with bisulfite modification as follows: genomic DNA was denatured in 3 mol l-1 NaOH for 15 min at 37 °C, and the cytosines were sulfonated in 3.6 mol l-1 sodium bisulfite and 1 mmol l-1 hydroquinone (Sigma-Aldrich) for 16 h at 55 °C. .. The modified DNA samples were desalted using a DNA clean-up system (Promega Corporation, Fitchburg, WI, USA).

    Article Title: MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients
    Article Snippet: .. Methylation-specific PCR for TP53 and MGMT promoter methylation analysis Sodium bisulfite modification of isolated genomic DNA was performed using the CpGenome DNA kit (Chemicon International Inc., Temecula, CA, USA) according to the manufacturer’s protocol. ..

    Incubation:

    Article Title: miR-31 and its host gene lncRNA LOC554202 are regulated by promoter hypermethylation in triple-negative breast cancer
    Article Snippet: .. Preparation of bisulfite-modified DNA for methylation analysis Genomic DNA (1-2 μg) was denatured in 0.3 M NaOH for 30 min at 42°C, and then the unmethylated cytosine residues were sulphonated by incubation in 3.12 M sodium bisulfite (pH 5.0; Sigma)/5 mM hydroquinone (Sigma, MO, USA) at 55°C for 16 h. The sulphonated DNA was recovered using the QIAquick Gel Extraction system (Qiagen, MA, USA), according to the manufacturer's recommendations. ..

    Article Title: 5-methylcytosine promotes mRNA export — NSUN2 as the methyltransferase and ALYREF as an m5C reader
    Article Snippet: .. The RNA pellet was resuspended in 100 μl bisulfite solution (pH 5.1), which is a 100:1 mixture of 40% sodium bisulfite (Sigma) and 600 μM hydroquinone (Sigma) and subjected to heat incubation at 75 °C for 4 h. The reaction mixture was desalted by twice passing it through Micro Bio-spin 6 chromatography columns (Bio-Rad) and then desulfonated by incubation with an equal volume of 1 M Tris (pH 9.0) at 75 °C for 1 h. After ethanol precipitation, the RNAs were resuspended in 10 μl of RNase-free water and used for library construction or validation. .. Sanger sequencing of PCR products For the validation of methylated sites, a 300 ng aliquot of sodium bisulfite converted RNA was reverse transcribed into cDNA using ACT random hexamers and Superscript III Reverse Transcriptase Kit (Invitrogen) according to the manufacturer's instructions. cDNA was amplified by PCR using normal primers for untreated mRNAs and specific primers for bisulfite-treated mRNAs.

    Article Title: Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors
    Article Snippet: .. DNA was denatured by 0.3 M NaOH at 42°C for 30 min. Then 3 M sodium bisulfite (Sigma) and 10 mmol/L hydroquinone were added, which was further incubated at 50°C for 16 h. Modified DNA was purified using Wizard DNA purification resin (Promega), re-treated with 0.3 M NaOH at 37°C for 15 min, precipitated with ethanol, and resuspended in 50 μL water. ..

    Article Title: Metallothionein 1G functions as a tumor suppressor in thyroid cancer through modulating the PI3K/Akt signaling pathway
    Article Snippet: .. The mixture was then incubated for 2 h at 70°C in 500 μL of a freshly prepared solution containing 3 M sodium bisulfite (Sigma, Saint Louis, MO) and 10 mM hydroquinone (Sigma, Saint Louis, MO). .. DNA was subsequently purified with a Wizard DNA Clean-Up System (Promega Corp., Madison, WI) following the instructions of the manufacturer, followed by ethanol precipitation, dry, and resuspension in 50 μL of deionized H2 O. Bisulfited-treated DNA samples were stored at −80°C until use.

    Article Title: RTA Promoter Demethylation and Histone Acetylation Regulation of Murine Gammaherpesvirus 68 Reactivation
    Article Snippet: .. The denatured DNA was mixed with melted 2% (w/v) LMP agarose (Amresco) to form beads, and incubated with 2.5 M sodium bisulfite (Sigma) at pH 5.0 and 0.5 mM hydroquinone (Sigma) at 50°C for 4 hrs. .. The beads were washed by TE to remove agarose, incubated with 0.2 M NaOH for 30 min for desulfonation, and washed again by TE to remove NaOH solution.

    DNA Purification:

    Article Title: Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors
    Article Snippet: .. DNA was denatured by 0.3 M NaOH at 42°C for 30 min. Then 3 M sodium bisulfite (Sigma) and 10 mmol/L hydroquinone were added, which was further incubated at 50°C for 16 h. Modified DNA was purified using Wizard DNA purification resin (Promega), re-treated with 0.3 M NaOH at 37°C for 15 min, precipitated with ethanol, and resuspended in 50 μL water. ..

    Isolation:

    Article Title: MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients
    Article Snippet: .. Methylation-specific PCR for TP53 and MGMT promoter methylation analysis Sodium bisulfite modification of isolated genomic DNA was performed using the CpGenome DNA kit (Chemicon International Inc., Temecula, CA, USA) according to the manufacturer’s protocol. ..

    Polymerase Chain Reaction:

    Article Title: MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients
    Article Snippet: .. Methylation-specific PCR for TP53 and MGMT promoter methylation analysis Sodium bisulfite modification of isolated genomic DNA was performed using the CpGenome DNA kit (Chemicon International Inc., Temecula, CA, USA) according to the manufacturer’s protocol. ..

    Gel Extraction:

    Article Title: miR-31 and its host gene lncRNA LOC554202 are regulated by promoter hypermethylation in triple-negative breast cancer
    Article Snippet: .. Preparation of bisulfite-modified DNA for methylation analysis Genomic DNA (1-2 μg) was denatured in 0.3 M NaOH for 30 min at 42°C, and then the unmethylated cytosine residues were sulphonated by incubation in 3.12 M sodium bisulfite (pH 5.0; Sigma)/5 mM hydroquinone (Sigma, MO, USA) at 55°C for 16 h. The sulphonated DNA was recovered using the QIAquick Gel Extraction system (Qiagen, MA, USA), according to the manufacturer's recommendations. ..

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  • 97
    Millipore sodium bisulfite treated dna
    Sodium Bisulfite Treated Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium bisulfite treated dna/product/Millipore
    Average 97 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    sodium bisulfite treated dna - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

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