soc broth  (Millipore)


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  • 99
    Name:
    SOC Broth
    Description:

    Catalog Number:
    85469
    Price:
    None
    Applications:
    SOC Broth is a sterile solution for the recovery of electroporated Escherichia coli cells.
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    Structured Review

    Millipore soc broth

    https://www.bioz.com/result/soc broth/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soc broth - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: Selected cleaned PCR products were cloned into TOPO-TA Cloning® (Invitrogen, Carlsbad, California, USA) vectors according to the manufacturer’s instructions, except that the ligation reactions were halved. .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours.

    Amplification:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: The ITS region for members of supersection Cieca was sequenced directly from the cleaned amplified product in the DNA Sequencing Core Facility on the University of Florida campus (DSEQ, UF) with ABI 377 and ABI 373A automated sequencers. .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours.

    Ligation:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: Selected cleaned PCR products were cloned into TOPO-TA Cloning® (Invitrogen, Carlsbad, California, USA) vectors according to the manufacturer’s instructions, except that the ligation reactions were halved. .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours.

    Polymerase Chain Reaction:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: Selected cleaned PCR products were cloned into TOPO-TA Cloning® (Invitrogen, Carlsbad, California, USA) vectors according to the manufacturer’s instructions, except that the ligation reactions were halved. .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours.

    Incubation:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours. .. Only large white colonies, representing potentially recombinant plasmids, were selected for amplification and sequencing.

    DNA Sequencing:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: The ITS region for members of supersection Cieca was sequenced directly from the cleaned amplified product in the DNA Sequencing Core Facility on the University of Florida campus (DSEQ, UF) with ABI 377 and ABI 373A automated sequencers. .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours.

    Sequencing:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: Sequences were edited and assembled using the ABI software packages Sequence Navigator ™ Version 1.0.1 and Auto Assembler ™ Version 1.3.0 on an Apple PowerMac computer and aligned visually. .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours.

    Transformation Assay:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours. .. Only large white colonies, representing potentially recombinant plasmids, were selected for amplification and sequencing.

    Recombinant:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours. .. Only large white colonies, representing potentially recombinant plasmids, were selected for amplification and sequencing.

    Software:

    Article Title: A revision of PassifloraL.subgenusDecaloba(DC.) Rchb.supersectionCieca (Medik.) J. M. MacDougal Feuillet ( Passifloraceae)
    Article Snippet: Sequences were edited and assembled using the ABI software packages Sequence Navigator ™ Version 1.0.1 and Auto Assembler ™ Version 1.3.0 on an Apple PowerMac computer and aligned visually. .. Transformation reactions were incubated in SOC broth (2.0% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 -6H2 O, 20 mM glucose) at 37 °C for one hour before being spread onto figs containing S-Gal™/LB Agar/Kanamycin Blend (Sigma, St. Louis, Missouri, USA) and incubated at 37 °C for 8–18 hours.