symphony peptide synthesizer  (Gyros Protein Technologies)


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    Structured Review

    Gyros Protein Technologies symphony peptide synthesizer
    Symphony Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/symphony peptide synthesizer/product/Gyros Protein Technologies
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    symphony peptide synthesizer - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Intranasal Immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of Human Papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection
    Article Snippet: All the peptides were prepared in the institutional antigen-core facility utilizing FMOC solid phase chemistry on a PTI Symphony Peptide Synthesizer (Protein Technologies Inc., Tucson, Arizona). .. Peptide purity was determined to be > 95% by high-pressure liquid chromatography (HPLC) and was validated by mass spectrometry.

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). .. The peptides were oxidized overnight in 0.1 M ammonium bicarbonate buffer at pH 8.5 and purified using reverse-phase HPLC (solvent A: H2 O, 0.05% (v /v ) trifluoroacetic acid (TFA), solvent B: 90% (v /v ) acetonitrile, 0.05% (v /v ) TFA) until the desired purity of > 95%.

    Article Title: Combination of PET and CXCR4-Targeted Peptide Molecule Agents for Noninvasive Tumor Monitoring
    Article Snippet: CXCR4 agent synthesis (NIR dye- leu-gly-ala-ser-trp-his-arg-pro-asp-lys-ala-cys-leu-gly-tyr-gln-lys-arg-pro-leu-pro-NH2 ): d-amino-peptide LGASWHRPDKACLGYQKRPLP (DVI-C11A ) was prepared following standard procedures for Fmoc solid-phase peptide synthesis on a Symphony synthesizer (Protein Technologies, Inc., Tucson, AZ). .. The compound was cleaved from the support using trifluoroacetic acid/water/triethylsilane (94/2/4) and purified by reverse phase HPLC.

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Article Title: Oral Immunization of Rhesus Macaques with Adenoviral HIV Vaccines Using Enteric-coated Capsules
    Article Snippet: .. The peptides were prepared in the institutional antigen-core facility utilizing FMOC solid phase chemistry on a PTI Symphony Peptide Synthesizer (Protein Technologies Inc., Tucson, AZ) and the purity of the peptides was determined to be > 95% by high-pressure liquid chromatography and was validated by mass spectrometry. .. A maximum of 20 μl per capsule of Ad vectors dissolved in potassium phosphate buffered saline (KPBS) with 1.0 M sucrose [ ] were flash frozen in clear Quali-V® HPMC capsules (Size 2, Qualicaps Inc., Whitsett, NC) on dry ice, placed in 1.5-ml microcentrifuge tubes, wrapped with weighing paper, and immediately placed in a pre-chilled LabConco 300-ml flask.

    Conjugation Assay:

    Article Title: Combination of PET and CXCR4-Targeted Peptide Molecule Agents for Noninvasive Tumor Monitoring
    Article Snippet: CXCR4 agent synthesis (NIR dye- leu-gly-ala-ser-trp-his-arg-pro-asp-lys-ala-cys-leu-gly-tyr-gln-lys-arg-pro-leu-pro-NH2 ): d-amino-peptide LGASWHRPDKACLGYQKRPLP (DVI-C11A ) was prepared following standard procedures for Fmoc solid-phase peptide synthesis on a Symphony synthesizer (Protein Technologies, Inc., Tucson, AZ). .. The conjugation of NIR-dye-NHS to the peptide was carried out on the solid support in presence of N, N-diisopropylethylamine (10%) in DMF.

    Mass Spectrometry:

    Article Title: Intranasal Immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of Human Papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection
    Article Snippet: All the peptides were prepared in the institutional antigen-core facility utilizing FMOC solid phase chemistry on a PTI Symphony Peptide Synthesizer (Protein Technologies Inc., Tucson, Arizona). .. Peptide purity was determined to be > 95% by high-pressure liquid chromatography (HPLC) and was validated by mass spectrometry.

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Article Title: Oral Immunization of Rhesus Macaques with Adenoviral HIV Vaccines Using Enteric-coated Capsules
    Article Snippet: .. The peptides were prepared in the institutional antigen-core facility utilizing FMOC solid phase chemistry on a PTI Symphony Peptide Synthesizer (Protein Technologies Inc., Tucson, AZ) and the purity of the peptides was determined to be > 95% by high-pressure liquid chromatography and was validated by mass spectrometry. .. A maximum of 20 μl per capsule of Ad vectors dissolved in potassium phosphate buffered saline (KPBS) with 1.0 M sucrose [ ] were flash frozen in clear Quali-V® HPMC capsules (Size 2, Qualicaps Inc., Whitsett, NC) on dry ice, placed in 1.5-ml microcentrifuge tubes, wrapped with weighing paper, and immediately placed in a pre-chilled LabConco 300-ml flask.

    In Situ:

    Article Title: Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula
    Article Snippet: Chain assembly was performed following a previously established in situ neutralization protocol . .. The process was carried out using a Symphony Automatic Peptide Synthesizer (Protein Technologies, Inc., Tucson, AZ). sOAIP-1 was then de-protected and cleaved from the solid resin with a solution of TFA∶TIPS∶water at 90∶5∶5 ratio for 3 h and evaporated under a stream of N2 .

    Synthesized:

    Article Title: Novel Bcl-2 Homology-3 Domain-like Sequences Identified from Screening Randomized Peptide Libraries for Inhibitors of the Pro-survival Bcl-2 Proteins *
    Article Snippet: .. All regular peptides were synthesized on The Symphony® (Protein Technologies, Inc). ..

    Article Title: Interaction of Tarantula Venom Peptide ProTx-II with Lipid Membranes Is a Prerequisite for Its Inhibition of Human Voltage-gated Sodium Channel NaV1.7 *
    Article Snippet: .. ProTx-II and analogues ( ) were synthesized using Fmoc ( N -(9-fluorenyl)methoxycarbonyl) chemistry at 0.25-mmol scale on 2-chlorotrityl resin using an automatic synthesizer (Symphony, Protein Technologies Inc.). ..

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula
    Article Snippet: Triisopropylsilane (TIPS) and diethylether (Sigma-Aldrich), and acetonitrile (Merck) were obtained from commercial suppliers. sOAIP-1 was synthesized on Wang polystyrene resin preloaded with the first C-terminal amino acid residue (0.2 mmol/g). .. The process was carried out using a Symphony Automatic Peptide Synthesizer (Protein Technologies, Inc., Tucson, AZ). sOAIP-1 was then de-protected and cleaved from the solid resin with a solution of TFA∶TIPS∶water at 90∶5∶5 ratio for 3 h and evaporated under a stream of N2 .

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: .. Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). ..

    Article Title: Tag-Mass: Specific Molecular Imaging of Transcriptome and Proteome by Mass Spectrometry Based on Photocleavable Tag
    Article Snippet: .. The peptide is synthesized on Symphony (Protein Technologies, Inc.) and purified on a Delta-Pak C18, 15 μ m, 100 Å column (Waters). ..

    Article Title: Semienzymatic Cyclization of Disulfide-rich Peptides Using Sortase A *
    Article Snippet: .. The linear peptides [GGG]kB1[TGG], [G]Vc1.1[GLPETGGS], and [GG]SFTI-1[LPETGG] were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony®, Protein Technologies, Inc.) following standard Fmoc chemistry on a 2-chlorotrityl chloride resin. ..

    Article Title: Immunogenicity in mice and non-human primates of the Group A Streptococcal J8 peptide vaccine candidate conjugated to CRM197
    Article Snippet: .. Briefly, the peptide was synthesized by solid phase chemistry on a NovaPEG Rink Amide resin (0.62 mmeq/g, EMD Biosciences) using a Symphony Synthesizer (Protein Technologies, Inc.). ..

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: .. Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. 2-Chlorotrityl chloride resin was used for [GGG] PVIIA[LPETGG], whereas rink amide resin was used for linear κ-PVIIA to achieve C-terminal amidation. κ-PVIIA with N-terminal acetylation was produced by treating the resin with 6% acetic acid/0.2 M DIPEA in DMF following completion of elongation of the peptide.

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: .. The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.). .. The peptide chain was assembled on 2-chlorotrityl chloride resin (Novabiochem), piperidine was used for the deprotection step, and coupling of the Fmoc-protected amino acids was carried out as described for Boc synthesis.

    Neutralization:

    Article Title: Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula
    Article Snippet: Chain assembly was performed following a previously established in situ neutralization protocol . .. The process was carried out using a Symphony Automatic Peptide Synthesizer (Protein Technologies, Inc., Tucson, AZ). sOAIP-1 was then de-protected and cleaved from the solid resin with a solution of TFA∶TIPS∶water at 90∶5∶5 ratio for 3 h and evaporated under a stream of N2 .

    Ligation:

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.). .. The θ-defensin analogues were synthesized from C to N terminus, with the starting point for synthesis chosen to give an N-terminal cysteine residue (Cys14 for BTD-2, [Aba3,16 ]BTD-2, [Aba3,7,12,16 ]BTD-2, and l-BTD-2, and Cys16 for [Aba5,14 ]BTD-2 and [Aba5,7,12,14 ]BTD-2), to allow for cyclization by native chemical ligation ( ).

    CTL Assay:

    Article Title: Intranasal Immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of Human Papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection
    Article Snippet: In addition to these peptides, the E749–57 peptide, R9F, (RAHVYNIVTF) and the E649–58 peptide, V10C, (VYDFAFRDLC), that represent murine H2b -restricted CTL epitopes, were used in the CTL assays. .. All the peptides were prepared in the institutional antigen-core facility utilizing FMOC solid phase chemistry on a PTI Symphony Peptide Synthesizer (Protein Technologies Inc., Tucson, Arizona).

    Mouse Assay:

    Article Title: Intranasal Immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of Human Papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection
    Article Snippet: The E744–62 peptide, Q19D, (QAEPDRAHVYNIVTFCCKCD) and the E643–57 peptide, Q15L, (QLLRREVYDFAFRDL) were used for immunization of mice. .. All the peptides were prepared in the institutional antigen-core facility utilizing FMOC solid phase chemistry on a PTI Symphony Peptide Synthesizer (Protein Technologies Inc., Tucson, Arizona).

    Produced:

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. 2-Chlorotrityl chloride resin was used for [GGG] PVIIA[LPETGG], whereas rink amide resin was used for linear κ-PVIIA to achieve C-terminal amidation. κ-PVIIA with N-terminal acetylation was produced by treating the resin with 6% acetic acid/0.2 M DIPEA in DMF following completion of elongation of the peptide.

    Activation Assay:

    Article Title: Chemical Synthesis, Proper Folding, Nav Channel Selectivity Profile and Analgesic Properties of the Spider Peptide Phlotoxin 1
    Article Snippet: Chemical Synthesis of PhlTx1 Linear PhlTx1 was assembled stepwise using fmoc solid-phase chemistry on a Symphony Synthesizer (Protein Technologies Inc., Tucson, AZ, USA) at a 0.1 mmol scale on 2-chlorotrityl chloride resin (substitution approx. .. Fmoc protecting groups were removed using 20% piperidine in dimetilformamid (DMF) and free amine was coupled using tenfold excess of Fmoc amino acids and HCTU/DIEA activation in NMP/DMF (3 times 15 min).

    Nuclear Magnetic Resonance:

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). .. The correct peptide mass was confirmed with ESI-MS, while native disulfide connectivity was inferred from the dispersion of peaks in the 1D NMR spectra using a Bruker Avance 600 MHz spectrometer (Billerica, USA).

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: Paragraph title: Peptide Synthesis and NMR Spectroscopy ... The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.).

    Spectroscopy:

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: Paragraph title: Peptide Synthesis and NMR Spectroscopy ... The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.).

    Purification:

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula
    Article Snippet: The process was carried out using a Symphony Automatic Peptide Synthesizer (Protein Technologies, Inc., Tucson, AZ). sOAIP-1 was then de-protected and cleaved from the solid resin with a solution of TFA∶TIPS∶water at 90∶5∶5 ratio for 3 h and evaporated under a stream of N2 . .. Crude peptide solutions were lyophilized. sOAIP-1 was then purified via RP-HPLC using a linear acetonitrile gradient (15–40% Solvent B over 25 min); the toxin eluted at ∼28% Solvent B.

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Paragraph title: 4.1. Peptide Synthesis, Folding and Purification ... Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA).

    Article Title: Tag-Mass: Specific Molecular Imaging of Transcriptome and Proteome by Mass Spectrometry Based on Photocleavable Tag
    Article Snippet: .. The peptide is synthesized on Symphony (Protein Technologies, Inc.) and purified on a Delta-Pak C18, 15 μ m, 100 Å column (Waters). ..

    Article Title: Combination of PET and CXCR4-Targeted Peptide Molecule Agents for Noninvasive Tumor Monitoring
    Article Snippet: CXCR4 agent synthesis (NIR dye- leu-gly-ala-ser-trp-his-arg-pro-asp-lys-ala-cys-leu-gly-tyr-gln-lys-arg-pro-leu-pro-NH2 ): d-amino-peptide LGASWHRPDKACLGYQKRPLP (DVI-C11A ) was prepared following standard procedures for Fmoc solid-phase peptide synthesis on a Symphony synthesizer (Protein Technologies, Inc., Tucson, AZ). .. The compound was cleaved from the support using trifluoroacetic acid/water/triethylsilane (94/2/4) and purified by reverse phase HPLC.

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Article Title: In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization
    Article Snippet: Paragraph title: Peptide synthesis and purification. ... 9-Fluorenylmethoxycarbonyl (Fmoc)-based chemistry was used to synthesize linear peptides on an automated synthesizer (Symphony; Protein Technologies, Inc.).

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  • 95
    Gyros Protein Technologies symphony peptide synthesizer
    Symphony Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/symphony peptide synthesizer/product/Gyros Protein Technologies
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    symphony peptide synthesizer - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

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