synthesizer  (Gyros Protein Technologies)


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    Name:
    Symphony 115 VAC 60 Hz
    Description:
    Symphony Automated 12 Channel Solid Phase Peptide Synthesizer 110 VAC 50 60 Hz Complete with computer Windows XP Symphony Windows software color flat screen monitor keyboard mouse printer cables and connectors PTI Peptide Predictor software set of 12 chemically resistant O rings safety shield solvent cabinet 20 L pressure vessel single 20 L waste container solvent bottles 1 x 4 L 1 x 500 mL 3 x 1 L standard one year warranty
    Catalog Number:
    smps-110
    Price:
    None
    Applications:
    Peptide Synthesis, Organic Synthesis
    Category:
    Instruments
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    Structured Review

    Gyros Protein Technologies synthesizer
    Symphony Automated 12 Channel Solid Phase Peptide Synthesizer 110 VAC 50 60 Hz Complete with computer Windows XP Symphony Windows software color flat screen monitor keyboard mouse printer cables and connectors PTI Peptide Predictor software set of 12 chemically resistant O rings safety shield solvent cabinet 20 L pressure vessel single 20 L waste container solvent bottles 1 x 4 L 1 x 500 mL 3 x 1 L standard one year warranty
    https://www.bioz.com/result/synthesizer/product/Gyros Protein Technologies
    Average 95 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    synthesizer - by Bioz Stars, 2020-03
    95/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). .. The peptides were oxidized overnight in 0.1 M ammonium bicarbonate buffer at pH 8.5 and purified using reverse-phase HPLC (solvent A: H2 O, 0.05% (v /v ) trifluoroacetic acid (TFA), solvent B: 90% (v /v ) acetonitrile, 0.05% (v /v ) TFA) until the desired purity of > 95%.

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Mass Spectrometry:

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Ligation:

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.). .. The θ-defensin analogues were synthesized from C to N terminus, with the starting point for synthesis chosen to give an N-terminal cysteine residue (Cys14 for BTD-2, [Aba3,16 ]BTD-2, [Aba3,7,12,16 ]BTD-2, and l-BTD-2, and Cys16 for [Aba5,14 ]BTD-2 and [Aba5,7,12,14 ]BTD-2), to allow for cyclization by native chemical ligation ( ).

    Synthesized:

    Article Title: Novel Bcl-2 Homology-3 Domain-like Sequences Identified from Screening Randomized Peptide Libraries for Inhibitors of the Pro-survival Bcl-2 Proteins *
    Article Snippet: .. All regular peptides were synthesized on The Symphony® (Protein Technologies, Inc). ..

    Article Title: Interaction of Tarantula Venom Peptide ProTx-II with Lipid Membranes Is a Prerequisite for Its Inhibition of Human Voltage-gated Sodium Channel NaV1.7 *
    Article Snippet: .. ProTx-II and analogues ( ) were synthesized using Fmoc ( N -(9-fluorenyl)methoxycarbonyl) chemistry at 0.25-mmol scale on 2-chlorotrityl resin using an automatic synthesizer (Symphony, Protein Technologies Inc.). ..

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: .. Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). ..

    Article Title: Tag-Mass: Specific Molecular Imaging of Transcriptome and Proteome by Mass Spectrometry Based on Photocleavable Tag
    Article Snippet: .. The peptide is synthesized on Symphony (Protein Technologies, Inc.) and purified on a Delta-Pak C18, 15 μ m, 100 Å column (Waters). ..

    Article Title: Semienzymatic Cyclization of Disulfide-rich Peptides Using Sortase A *
    Article Snippet: .. The linear peptides [GGG]kB1[TGG], [G]Vc1.1[GLPETGGS], and [GG]SFTI-1[LPETGG] were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony®, Protein Technologies, Inc.) following standard Fmoc chemistry on a 2-chlorotrityl chloride resin. ..

    Article Title: Immunogenicity in mice and non-human primates of the Group A Streptococcal J8 peptide vaccine candidate conjugated to CRM197
    Article Snippet: .. Briefly, the peptide was synthesized by solid phase chemistry on a NovaPEG Rink Amide resin (0.62 mmeq/g, EMD Biosciences) using a Symphony Synthesizer (Protein Technologies, Inc.). ..

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: .. Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. 2-Chlorotrityl chloride resin was used for [GGG] PVIIA[LPETGG], whereas rink amide resin was used for linear κ-PVIIA to achieve C-terminal amidation. κ-PVIIA with N-terminal acetylation was produced by treating the resin with 6% acetic acid/0.2 M DIPEA in DMF following completion of elongation of the peptide.

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: .. The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.). .. The peptide chain was assembled on 2-chlorotrityl chloride resin (Novabiochem), piperidine was used for the deprotection step, and coupling of the Fmoc-protected amino acids was carried out as described for Boc synthesis.

    Purification:

    Article Title: ERAP1 enzyme-mediated trimming and structural analyses of MHC I–bound precursor peptides yield novel insights into antigen processing and presentation
    Article Snippet: .. Synthetic peptides Peptides were synthesized by the solid-phase methodology on a Symphony synthesizer (Protein Technologies Inc.) and purified by reverse-phase HPLC on a C18 Agilent column. ..

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Paragraph title: 4.1. Peptide Synthesis, Folding and Purification ... Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA).

    Article Title: Tag-Mass: Specific Molecular Imaging of Transcriptome and Proteome by Mass Spectrometry Based on Photocleavable Tag
    Article Snippet: .. The peptide is synthesized on Symphony (Protein Technologies, Inc.) and purified on a Delta-Pak C18, 15 μ m, 100 Å column (Waters). ..

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. Peptides were purified using reversed-phase high performance liquid chromatography (RP-HPLC) and the mass was confirmed using mass spectrometry.

    Article Title: In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization
    Article Snippet: Paragraph title: Peptide synthesis and purification. ... 9-Fluorenylmethoxycarbonyl (Fmoc)-based chemistry was used to synthesize linear peptides on an automated synthesizer (Symphony; Protein Technologies, Inc.).

    Produced:

    Article Title: Efficient Enzymatic Cyclization of an Inhibitory Cystine Knot-Containing Peptide
    Article Snippet: Linear peptides were synthesized by solid-phase peptide synthesis using an automatic peptide synthesizer (Symphony® , Protein Technologies, Inc., Tucson, AZ) following standard Fmoc chemistry protocols. .. 2-Chlorotrityl chloride resin was used for [GGG] PVIIA[LPETGG], whereas rink amide resin was used for linear κ-PVIIA to achieve C-terminal amidation. κ-PVIIA with N-terminal acetylation was produced by treating the resin with 6% acetic acid/0.2 M DIPEA in DMF following completion of elongation of the peptide.

    Activation Assay:

    Article Title: Chemical Synthesis, Proper Folding, Nav Channel Selectivity Profile and Analgesic Properties of the Spider Peptide Phlotoxin 1
    Article Snippet: Chemical Synthesis of PhlTx1 Linear PhlTx1 was assembled stepwise using fmoc solid-phase chemistry on a Symphony Synthesizer (Protein Technologies Inc., Tucson, AZ, USA) at a 0.1 mmol scale on 2-chlorotrityl chloride resin (substitution approx. .. Fmoc protecting groups were removed using 20% piperidine in dimetilformamid (DMF) and free amine was coupled using tenfold excess of Fmoc amino acids and HCTU/DIEA activation in NMP/DMF (3 times 15 min).

    Nuclear Magnetic Resonance:

    Article Title: Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties
    Article Snippet: Briefly, the peptides were synthesized using 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase peptide synthesis (SPPS) on an automatic peptide synthesizer (Symphony, Protein Technologies Inc., Tucson, USA). .. The correct peptide mass was confirmed with ESI-MS, while native disulfide connectivity was inferred from the dispersion of peaks in the 1D NMR spectra using a Bruker Avance 600 MHz spectrometer (Billerica, USA).

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: Paragraph title: Peptide Synthesis and NMR Spectroscopy ... The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.).

    Spectroscopy:

    Article Title: The Cyclic Cystine Ladder in ?-Defensins Is Important for Structure and Stability, but Not Antibacterial Activity *
    Article Snippet: Paragraph title: Peptide Synthesis and NMR Spectroscopy ... The acyclic θ-defensin analogue, BTD-2[ 3,4 ], in which the cyclic peptide backbone was broken between residues Cys3 and Arg4 , was synthesized using Fmoc chemistry on an automated synthesizer (Symphony; Protein Technologies, Inc.).

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  • 99
    Gyros Protein Technologies symphony peptide synthesizer
    Symphony Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/symphony peptide synthesizer/product/Gyros Protein Technologies
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    symphony peptide synthesizer - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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