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Horizon Discovery smartpool deconvoluted sirna
Kinome <t>siRNA</t> screen to determine genes involved in AT 1 R–EGFR transactivation. (A) Using the HMEC-LST-AT 1 R cell line, we performed a primary siRNA screen using the Dharmacon siGENOME <t>SMARTpool</t> siRNA library targeting kinase genes (720 in total,
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1) Product Images from "A functional siRNA screen identifies genes modulating angiotensin II-mediated EGFR transactivation"

Article Title: A functional siRNA screen identifies genes modulating angiotensin II-mediated EGFR transactivation

Journal: Journal of Cell Science

doi: 10.1242/jcs.128280

Kinome siRNA screen to determine genes involved in AT 1 R–EGFR transactivation. (A) Using the HMEC-LST-AT 1 R cell line, we performed a primary siRNA screen using the Dharmacon siGENOME SMARTpool siRNA library targeting kinase genes (720 in total,
Figure Legend Snippet: Kinome siRNA screen to determine genes involved in AT 1 R–EGFR transactivation. (A) Using the HMEC-LST-AT 1 R cell line, we performed a primary siRNA screen using the Dharmacon siGENOME SMARTpool siRNA library targeting kinase genes (720 in total,

Techniques Used:

Related Articles

Concentration Assay:

Article Title: A functional siRNA screen identifies genes modulating angiotensin II-mediated EGFR transactivation
Article Snippet: .. Briefly, 40 nM Dharmacon SMARTpool siRNA or 25 nM Dharmacon SMARTpool deconvoluted siRNA (individual duplex) were complexed for 20 minutes at ambient temperature in a 200 µl volume of HuMEC basal medium with a final concentration of 1 µl per well of DharmaFECT 1 transfection lipid (ThermoFisher Scientific). .. HMEC-LST-AT1 R cells (∼100,000) in antibiotic-free HuMEC complete medium (800 µl volume) were seeded into each well along with the complexed siRNA and incubated for 24 hours.

Transfection:

Article Title: A functional siRNA screen identifies genes modulating angiotensin II-mediated EGFR transactivation
Article Snippet: .. Briefly, 40 nM Dharmacon SMARTpool siRNA or 25 nM Dharmacon SMARTpool deconvoluted siRNA (individual duplex) were complexed for 20 minutes at ambient temperature in a 200 µl volume of HuMEC basal medium with a final concentration of 1 µl per well of DharmaFECT 1 transfection lipid (ThermoFisher Scientific). .. HMEC-LST-AT1 R cells (∼100,000) in antibiotic-free HuMEC complete medium (800 µl volume) were seeded into each well along with the complexed siRNA and incubated for 24 hours.