small unilamellar vesicle suv preparation lipids  (Croda International Plc)

Journal: European Biophysics Journal

Article Title: The effect of C-terminal deamidation on bacterial susceptibility and resistance to modelin-5

doi: 10.1007/s00249-025-01732-4

Figure Lengend Snippet: The binding of M5-NH isoforms to bacterial CMs. Figure 1 shows the change in fluorescence (ΔF) induced by increasing concentrations of M5-NH with FPE-labelled lipid SUVs that represented the P. aeruginosa CM (black line) and the S. aureus CM (dashed black line) (Table ). Also shown are corresponding changes in fluorescence induced by M5-OH with FPE-labelled lipid SUVs representing the P. aeruginosa CM (grey line) and the S. aureus CM (dashed grey line). In each case, analysis of these curves was used to derive K d (Table A), as described above, and error bars represent the standard deviation (Maman )

Article Snippet: The CM of P. aeruginosa , strain NCIMB 8295, and S. aureus , strain NCIMB 6571, were represented by small unilamellar vesicles (SUVs) formed from lipid mixtures with the compositions shown in Table .

Techniques: Binding Assay, Fluorescence, Standard Deviation

Journal: European Biophysics Journal

Article Title: The effect of C-terminal deamidation on bacterial susceptibility and resistance to modelin-5

doi: 10.1007/s00249-025-01732-4

Figure Lengend Snippet: CD analysis of M5-NH isoforms in the presence of bacterial CMs. Figure 5 shows CD spectra for the conformational behaviour of M5-NH in the presence of lipid SUVs representing the P. aeruginosa CM, (black line) and the S. aureus CM, (dashed black line. Also shown are corresponding spectra for the conformational behaviour of M5-OH with lipid SUVs representing the P. aeruginosa CM (grey line) and S. aureus CM (dashed grey line) (Table ). Minima at 208 nm and 225 nm, and maxima at 190 nm are indicative of α-helical architecture, and in each case, these spectra were analysed and levels of α-helicity determined (Table A), as described above (Miles et al. )

Article Snippet: The CM of P. aeruginosa , strain NCIMB 8295, and S. aureus , strain NCIMB 6571, were represented by small unilamellar vesicles (SUVs) formed from lipid mixtures with the compositions shown in Table .

Techniques: Circular Dichroism

Journal: European Biophysics Journal

Article Title: The effect of C-terminal deamidation on bacterial susceptibility and resistance to modelin-5

doi: 10.1007/s00249-025-01732-4

Figure Lengend Snippet: The membranolytic action of M5-NH isoforms against bacterial CMs. Figure 4 shows the change in lysis levels induced by increasing concentrations of M5-NH with calcein loaded, lipid SUVs representing the P. aeruginosa CM (black line) and the S. aureus CM (dashed black line) (Table ). Also shown are corresponding changes in surface pressure induced by M5-OH with calcein loaded SUVs representing the P. aeruginosa CM (grey line) and the S. aureus CM (dashed grey line). In each case, maximal levels of lysis induced by peptides were determined (Table A) and error bars represent the standard deviation

Article Snippet: The CM of P. aeruginosa , strain NCIMB 8295, and S. aureus , strain NCIMB 6571, were represented by small unilamellar vesicles (SUVs) formed from lipid mixtures with the compositions shown in Table .

Techniques: Lysis, Standard Deviation