smai site  (Thermo Fisher)


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    Name:
    SmaI
    Description:
    5 C C C ↓G G G 3 3 G G G ↑C C C 5 Thermo Scientific SmaI restriction enzyme recognizes CCC GGG sites and cuts best at 30°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Isoschizomers TspMI XmaCI neoschizomer XmaI Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0663
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher smai site
    5 C C C ↓G G G 3 3 G G G ↑C C C 5 Thermo Scientific SmaI restriction enzyme recognizes CCC GGG sites and cuts best at 30°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Isoschizomers TspMI XmaCI neoschizomer XmaI Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/smai site/product/Thermo Fisher
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    smai site - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Functional Analysis of Drosophila HSP70 Promoter with Different HSE Numbers in Human Cells
    Article Snippet: .. The 600 bp EcoRV/BamHI fragment of pGEM/Gdnf containing the Gdnf gene was cloned into the EcoRV and SmaI sites of B7 and F7 using the Klenow fragment (Fermentas). .. The authenticity of the resulting constructs B7/Gdnf/gfp and F7/Gdnf/gfp was confirmed by restriction endonuclease analysis and sequencing.

    Article Title: Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants
    Article Snippet: .. PCR amplicons were cloned into PVX-containing pgR107 vector [ , ] in the SmaI restriction site, using T4 DNA ligase (Thermo Scientific, Waltham, MA, USA). ..

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Amplification:

    Article Title: The association of circulating adiponectin and + 45 T/G polymorphism of adiponectin gene with gestational diabetes mellitus in Iranian population
    Article Snippet: .. PCR thermal cycling was started with initial denaturation at 95°C for 5 min, followed with 35 cycles of amplification which included denaturation at 95°C for 30 sec, annealing at 58°C for 30 sec, extension at 72°C for 30 sec, and final extension at 72°C for 10 min. PCR products were digested by SmaI restriction enzyme (Fermentas Life Sciences, Lithuania) and separated on 2% agarose gel electrophoresis. ..

    Article Title: Saccharomyces cerevisiae-based system for studying clustered DNA damages
    Article Snippet: .. The plasmid pMM-25, was constructed by inserting the amplification product as a SmaI fragment (1,305 bp) into SmaI site in the MCS of plasmid pBluescript II SK ± (2,961 bp, Fermentas), thus generating a 4,266-bp vector (Supplementary Fig. 2B). .. The vector pMM-25, amplified in E. coli DH5α competent cells and sequenced, was used as the sole source in all our experiments.

    Article Title: Molecular epidemiology of hepatitis B and hepatitis delta viruses circulating in the Western Amazon region, North Brazil
    Article Snippet: .. For restriction fragment length polymorphism (RFLP) the amplification products were detected in 1.5% agarose gel and digested with restriction enzyme SmaI (5 U) (Life Technologies). .. Digested products were analyzed by electrophoresis through 6% acrylamide gels according to the size of the fragments: genotype 1 (227-178 bp), genotype 2 (no digestion), genotype 3 (298-107 bp) [ ].

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Agarose Gel Electrophoresis:

    Article Title: The association of circulating adiponectin and + 45 T/G polymorphism of adiponectin gene with gestational diabetes mellitus in Iranian population
    Article Snippet: .. PCR thermal cycling was started with initial denaturation at 95°C for 5 min, followed with 35 cycles of amplification which included denaturation at 95°C for 30 sec, annealing at 58°C for 30 sec, extension at 72°C for 30 sec, and final extension at 72°C for 10 min. PCR products were digested by SmaI restriction enzyme (Fermentas Life Sciences, Lithuania) and separated on 2% agarose gel electrophoresis. ..

    Article Title: Molecular epidemiology of hepatitis B and hepatitis delta viruses circulating in the Western Amazon region, North Brazil
    Article Snippet: .. For restriction fragment length polymorphism (RFLP) the amplification products were detected in 1.5% agarose gel and digested with restriction enzyme SmaI (5 U) (Life Technologies). .. Digested products were analyzed by electrophoresis through 6% acrylamide gels according to the size of the fragments: genotype 1 (227-178 bp), genotype 2 (no digestion), genotype 3 (298-107 bp) [ ].

    Stable Transfection:

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Size-exclusion Chromatography:

    Article Title: The association of circulating adiponectin and + 45 T/G polymorphism of adiponectin gene with gestational diabetes mellitus in Iranian population
    Article Snippet: .. PCR thermal cycling was started with initial denaturation at 95°C for 5 min, followed with 35 cycles of amplification which included denaturation at 95°C for 30 sec, annealing at 58°C for 30 sec, extension at 72°C for 30 sec, and final extension at 72°C for 10 min. PCR products were digested by SmaI restriction enzyme (Fermentas Life Sciences, Lithuania) and separated on 2% agarose gel electrophoresis. ..

    Construct:

    Article Title: Saccharomyces cerevisiae-based system for studying clustered DNA damages
    Article Snippet: .. The plasmid pMM-25, was constructed by inserting the amplification product as a SmaI fragment (1,305 bp) into SmaI site in the MCS of plasmid pBluescript II SK ± (2,961 bp, Fermentas), thus generating a 4,266-bp vector (Supplementary Fig. 2B). .. The vector pMM-25, amplified in E. coli DH5α competent cells and sequenced, was used as the sole source in all our experiments.

    Polymerase Chain Reaction:

    Article Title: Nasopharyngeal carriage of Staphylococcus aureus among imprisoned males from Brazil without exposure to healthcare: risk factors and molecular characterization
    Article Snippet: .. Reactants for PCR and PFGE were acquired from the following manufacturers: Taq polymerase, Biotools (Madrid, Spain); PCR primers, Life Technologies (São Paulo, Brazil); SmaI restriction enzyme, Fermentas (Pittsburgh, PA). .. International clones kindly provided by Dr. Antonio Carlos Campos Pignatari, Laboratório Especial de Microbiologia Clínica, Disciplina de Infectologia, Universidade Federal de São Paulo/Escola Paulista de Medicina, and Dr. Agnes Marie Sá Figueiredo, Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Prof. Paulo de Góes, Brazil, were used as controls.

    Article Title: The association of circulating adiponectin and + 45 T/G polymorphism of adiponectin gene with gestational diabetes mellitus in Iranian population
    Article Snippet: .. PCR thermal cycling was started with initial denaturation at 95°C for 5 min, followed with 35 cycles of amplification which included denaturation at 95°C for 30 sec, annealing at 58°C for 30 sec, extension at 72°C for 30 sec, and final extension at 72°C for 10 min. PCR products were digested by SmaI restriction enzyme (Fermentas Life Sciences, Lithuania) and separated on 2% agarose gel electrophoresis. ..

    Article Title: Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants
    Article Snippet: .. PCR amplicons were cloned into PVX-containing pgR107 vector [ , ] in the SmaI restriction site, using T4 DNA ligase (Thermo Scientific, Waltham, MA, USA). ..

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Expressing:

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Modification:

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Western Blot:

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Transformation Assay:

    Article Title: Affimer proteins are versatile and renewable affinity reagents
    Article Snippet: .. The UL49 gene of Herpesvirus of Turkeys (HVT) was amplified by PCR using Q5 DNA polymerase (NEB, UK) and cloned using the Gibson Assembly kit (NEB) into a modified pMT-V5/6His (Invitrogen) in which the V5/6His cassette was replaced with 6His-AviTag and a SmaI site that was used to generate an N-terminal fusion to the UL49 gene product. pMT HVT UL49 was co-transfected with pCoHygro (Invitrogen) into Drosophila S2 cells using calcium phosphate precipitation and stably transformed cells were selected with hygromycin according to the manufacturer’s instructions (Invitrogen) before expression testing by western blot analysis 24 hr after induction with 500 µM copper sulphate. .. For purification, protein was extracted from stably transformed cells 36 hr after induction with copper sulphate at 20°C using a modified lysis buffer (25 mM Tris (pH8), 1.5% Triton-X100, 50 mM arginine (pH8), 10 mM imidazole, 7.5% glycerol, 300 mM KCl).

    Plasmid Preparation:

    Article Title: Saccharomyces cerevisiae-based system for studying clustered DNA damages
    Article Snippet: .. The plasmid pMM-25, was constructed by inserting the amplification product as a SmaI fragment (1,305 bp) into SmaI site in the MCS of plasmid pBluescript II SK ± (2,961 bp, Fermentas), thus generating a 4,266-bp vector (Supplementary Fig. 2B). .. The vector pMM-25, amplified in E. coli DH5α competent cells and sequenced, was used as the sole source in all our experiments.

    Article Title: Superinfection by PHYVV Alters the Recovery Process in PepGMV-Infected Pepper Plants
    Article Snippet: .. PCR amplicons were cloned into PVX-containing pgR107 vector [ , ] in the SmaI restriction site, using T4 DNA ligase (Thermo Scientific, Waltham, MA, USA). ..

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  • 94
    Thermo Fisher smai restriction site
    Smai Restriction Site, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smai restriction site/product/Thermo Fisher
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    smai restriction site - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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