smad7  (Boster Bio)


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    Name:
    Anti Smad7 Antibody
    Description:

    Catalog Number:
    A00784
    Price:
    412.0
    Category:
    Primary Antibodies
    Reactivity:
    Human Mouse Rat
    Applications:
    WB
    Host:
    Rabbit
    Buy from Supplier


    Structured Review

    Boster Bio smad7
    Anti Smad7 Antibody

    https://www.bioz.com/result/smad7/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    smad7 - by Bioz Stars, 2021-09
    86/100 stars

    Images

    1) Product Images from "Molecular responses of radiation-induced liver damage in rats"

    Article Title: Molecular responses of radiation-induced liver damage in rats

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2014.3051

    Immunohistochemical staining of liver sections 3 days and 1, 2, 4, 8 and 12 weeks after 20 Gy irradiation. Brown color denotes positivity. The perivenous area was the major area of positive staining. TGF-β1, Smad3, CTGF and NF-κB p65 staining was positive between 3 days and 12 weeks after irradiation. Smad4 and Smad7 staining was positive between 3 days and 1 week after irradiation. TNF-α staining was positive between 1 and 4 weeks after irradiation. (Magnification, ×200). Gy, grays; TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor; TNFα, tumor necrosis factor-α, NF, nuclear factor.
    Figure Legend Snippet: Immunohistochemical staining of liver sections 3 days and 1, 2, 4, 8 and 12 weeks after 20 Gy irradiation. Brown color denotes positivity. The perivenous area was the major area of positive staining. TGF-β1, Smad3, CTGF and NF-κB p65 staining was positive between 3 days and 12 weeks after irradiation. Smad4 and Smad7 staining was positive between 3 days and 1 week after irradiation. TNF-α staining was positive between 1 and 4 weeks after irradiation. (Magnification, ×200). Gy, grays; TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor; TNFα, tumor necrosis factor-α, NF, nuclear factor.

    Techniques Used: Immunohistochemistry, Staining, Irradiation

    Western blot analysis (ratio of the molecules investigated, vs. GAPDH). Similar to the results obtained using the reverse transcription quantitative polymerase chain reaction, the protein expression levels were as follows: NF-κB p65 was increased between 3 days and 12 weeks after irradiation. CTGF and Smad3 were significantly increased between 2 and 12 weeks after irradiation. TGF-β1 was significantly increased between 1 and 12 weeks after irradiation and TNF-α was significantly increased between 3 days and 4 weeks after irradiation. Smad4 was significantly increased between 3 days and 1 week after irradiation. Smad7 was significantly increased 3 days after irradiation and reduced significantly 1 and 2 weeks after irradiation, however, it remained higher than that in the control. From 4 weeks post-irradiation, the protein expression of Smad7 returned to the control level. * P
    Figure Legend Snippet: Western blot analysis (ratio of the molecules investigated, vs. GAPDH). Similar to the results obtained using the reverse transcription quantitative polymerase chain reaction, the protein expression levels were as follows: NF-κB p65 was increased between 3 days and 12 weeks after irradiation. CTGF and Smad3 were significantly increased between 2 and 12 weeks after irradiation. TGF-β1 was significantly increased between 1 and 12 weeks after irradiation and TNF-α was significantly increased between 3 days and 4 weeks after irradiation. Smad4 was significantly increased between 3 days and 1 week after irradiation. Smad7 was significantly increased 3 days after irradiation and reduced significantly 1 and 2 weeks after irradiation, however, it remained higher than that in the control. From 4 weeks post-irradiation, the protein expression of Smad7 returned to the control level. * P

    Techniques Used: Western Blot, Real-time Polymerase Chain Reaction, Expressing, Irradiation

    mRNA expression levels were determined using reverse transcription quantitative polymerase chain reaction (ratio of the molecules investigated, vs. GAPDH). The mRNA expression of NF-κB p65 was upregulated between 3 days and 12 weeks after irradiation. The mRNA expression levels were as follows: CTGF and Smad3 were significantly upregulated between 2 and 12 weeks after irradiation. TGF-β1 was significantly upregulated between 1 and 12 weeks after irradiation. TNF-α was significantly upregulated between 3 days and 4 weeks after irradiation. Smad4 was significantly upregulated 3 days and 1 week after irradiation. Smad7 was significantly upregulated 3 days after irradiation and reduced significantly 1–2 weeks after irradiation, however, the expression levels remained higher than that in the control. From 4 weeks post-irradiation, the mRNA expression of Smad7 returned to the control level. * P
    Figure Legend Snippet: mRNA expression levels were determined using reverse transcription quantitative polymerase chain reaction (ratio of the molecules investigated, vs. GAPDH). The mRNA expression of NF-κB p65 was upregulated between 3 days and 12 weeks after irradiation. The mRNA expression levels were as follows: CTGF and Smad3 were significantly upregulated between 2 and 12 weeks after irradiation. TGF-β1 was significantly upregulated between 1 and 12 weeks after irradiation. TNF-α was significantly upregulated between 3 days and 4 weeks after irradiation. Smad4 was significantly upregulated 3 days and 1 week after irradiation. Smad7 was significantly upregulated 3 days after irradiation and reduced significantly 1–2 weeks after irradiation, however, the expression levels remained higher than that in the control. From 4 weeks post-irradiation, the mRNA expression of Smad7 returned to the control level. * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Irradiation

    Related Articles

    other:

    Article Title: Role of transforming growth factor-beta signaling pathway in pathogenesis of benign biliary stricture
    Article Snippet: Antibodies to TGF-β1 (Rabbit anti-human monoclonal antibody, Santa-dilution 1:400), TβRI (Rabbit anti-human monoclonal antibody, Santa-dilution 1:400), TβRII (Rabbit anti-human monoclonal antibody, Santa-dilution 1:300), Smad4 (Rabbit anti-human monoclonal antibody, Santa-dilution 1:400), Smad7 (Rabbit anti-human monoclonal antibody, Santa-dilution 1:400) and CTGF (Rabbit anti-human monoclonal antibody, Santa-dilution 1:300) were purchased from Wuhan Boster Biological Technology Co. Ltd.

    Immunohistochemistry:

    Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury
    Article Snippet: .. According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H2 O2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C. ..

    Staining:

    Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury
    Article Snippet: .. According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H2 O2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C. ..

    Activity Assay:

    Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury
    Article Snippet: .. According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H2 O2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C. ..

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  • 92
    Boster Bio smad7 antibody
    <t>Smad7</t> protein expression in each group by Western blot. (a) Western blot strip chart. (b) The gray graph shows the relative statistical values of Smad7 for each group. Compared with the NC group, Smad7 expression decreased in the experimental groups, especially in the diabetic nephropathy group, with a subsequent increase in the MH and ML groups, and a more pronounced increase in the MH group compared with the ML group. ▲ P
    Smad7 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smad7 antibody/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    smad7 antibody - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    86
    Boster Bio smad7
    Immunohistochemical staining of liver sections 3 days and 1, 2, 4, 8 and 12 weeks after 20 Gy irradiation. Brown color denotes positivity. The perivenous area was the major area of positive staining. TGF-β1, Smad3, CTGF and NF-κB p65 staining was positive between 3 days and 12 weeks after irradiation. Smad4 and <t>Smad7</t> staining was positive between 3 days and 1 week after irradiation. TNF-α staining was positive between 1 and 4 weeks after irradiation. (Magnification, ×200). Gy, grays; TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor; TNFα, tumor necrosis factor-α, NF, nuclear factor.
    Smad7, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smad7/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    smad7 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Smad7 protein expression in each group by Western blot. (a) Western blot strip chart. (b) The gray graph shows the relative statistical values of Smad7 for each group. Compared with the NC group, Smad7 expression decreased in the experimental groups, especially in the diabetic nephropathy group, with a subsequent increase in the MH and ML groups, and a more pronounced increase in the MH group compared with the ML group. ▲ P

    Journal: Journal of Diabetes Research

    Article Title: MG132 Ameliorates Kidney Lesions by Inhibiting the Degradation of Smad7 in Streptozotocin-Induced Diabetic Nephropathy

    doi: 10.1155/2014/918396

    Figure Lengend Snippet: Smad7 protein expression in each group by Western blot. (a) Western blot strip chart. (b) The gray graph shows the relative statistical values of Smad7 for each group. Compared with the NC group, Smad7 expression decreased in the experimental groups, especially in the diabetic nephropathy group, with a subsequent increase in the MH and ML groups, and a more pronounced increase in the MH group compared with the ML group. ▲ P

    Article Snippet: Immunoblot analysis was performed using TGF-β antibody (rabbit, 1 : 1000; Cell Signaling Technology (CST), USA), Smurf2 antibody (rabbit, 1 : 1000; Abcam, USA), actin antibody (rabbit, 1 : 1000; Abcam, USA), Smad7 antibody (rabbit, 1 : 500; Boster Biological Technology, China).

    Techniques: Expressing, Western Blot, Stripping Membranes

    Smad7 mRNA levels by Real-Time fluorescent quantitative PCR in each group. (a) RT-PCR strip chart. (b) The gray graph shows the relative statistical values of the mRNA levels. There was not significant change of Smad7 mRNA levels in the different group.

    Journal: Journal of Diabetes Research

    Article Title: MG132 Ameliorates Kidney Lesions by Inhibiting the Degradation of Smad7 in Streptozotocin-Induced Diabetic Nephropathy

    doi: 10.1155/2014/918396

    Figure Lengend Snippet: Smad7 mRNA levels by Real-Time fluorescent quantitative PCR in each group. (a) RT-PCR strip chart. (b) The gray graph shows the relative statistical values of the mRNA levels. There was not significant change of Smad7 mRNA levels in the different group.

    Article Snippet: Immunoblot analysis was performed using TGF-β antibody (rabbit, 1 : 1000; Cell Signaling Technology (CST), USA), Smurf2 antibody (rabbit, 1 : 1000; Abcam, USA), actin antibody (rabbit, 1 : 1000; Abcam, USA), Smad7 antibody (rabbit, 1 : 500; Boster Biological Technology, China).

    Techniques: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Stripping Membranes

    Effects of echinacoside on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p

    Journal: Molecules

    Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β1/smad as Potential Anti-Hepatic Fibrosis Agents

    doi: 10.3390/molecules21010102

    Figure Lengend Snippet: Effects of echinacoside on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p

    Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Effects of CPhGs on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p

    Journal: Molecules

    Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β1/smad as Potential Anti-Hepatic Fibrosis Agents

    doi: 10.3390/molecules21010102

    Figure Lengend Snippet: Effects of CPhGs on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p

    Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Effects of echinacoside on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data were expressed as mean ± SD. ** p

    Journal: Molecules

    Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β1/smad as Potential Anti-Hepatic Fibrosis Agents

    doi: 10.3390/molecules21010102

    Figure Lengend Snippet: Effects of echinacoside on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data were expressed as mean ± SD. ** p

    Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

    Techniques: Expressing

    Effects of CPhGs on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data are expressed as mean ± SD. ** p

    Journal: Molecules

    Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β1/smad as Potential Anti-Hepatic Fibrosis Agents

    doi: 10.3390/molecules21010102

    Figure Lengend Snippet: Effects of CPhGs on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data are expressed as mean ± SD. ** p

    Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

    Techniques: Expressing

    Effects of acteoside on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data were expressed as mean ± SD. ** p

    Journal: Molecules

    Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β1/smad as Potential Anti-Hepatic Fibrosis Agents

    doi: 10.3390/molecules21010102

    Figure Lengend Snippet: Effects of acteoside on the protein expression of smad2, p-smad2, smad3, p-smad3 and smad7 in HSC. ( A ) Smad2 protein level; ( B ) P-smad2 protein level; ( C ) Smad3 protein level; ( D ) P-smad3 protein level; ( E ) Smad7 protein level. Data were expressed as mean ± SD. ** p

    Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

    Techniques: Expressing

    Effects of acteoside on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p

    Journal: Molecules

    Article Title: Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β1/smad as Potential Anti-Hepatic Fibrosis Agents

    doi: 10.3390/molecules21010102

    Figure Lengend Snippet: Effects of acteoside on the expressions of smad2, smad3 and smad7 in HSC (RT-PCR assay). Data were expressed as mean ± SD. ** p

    Article Snippet: Smad7 antibody was purchased from Boster (Wuhan, China).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Kuijie Granule increases the expression of Smad4 and decreases Smad7 in colon tissue of patients with UC. (a) and (b) show the data analysis results for Smad4 and Smad7 expression; there exist significant differences before and after Kuijie Granule treatment ( ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis

    doi: 10.1155/2016/2601830

    Figure Lengend Snippet: Kuijie Granule increases the expression of Smad4 and decreases Smad7 in colon tissue of patients with UC. (a) and (b) show the data analysis results for Smad4 and Smad7 expression; there exist significant differences before and after Kuijie Granule treatment ( ∗∗ P

    Article Snippet: Reagents Rabbit anti-TGF-β 1, rabbit anti-TGF-β RII, rabbit anti-Smad2, rabbit anti-Smad4, rabbit anti-Smad6, and rabbit anti-Smad7 monoclonal antibody were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Expressing

    Mechanisms of Kuijie Granule in suppressing ulcerative colitis. Kuijie Granule can improve the clinical symptoms (diarrhea, mucous bloody stool, abdominal pain, abdominal distention, and tenesmus) in UC patients by regulating TGF- β /Smads signal transduction pathway. Kuijie Granule decreased the expression of TGF- β 1, increased the expression of TGF- β RII, Smad4, and Smad7, and has no effect on Smad2 and Smad6 expression; then, the decreasing of the phosphorylation of anti-inflammation signal proteins and the blocking of the expression of inflammation signal proteins finally reduced inflammation reaction to improve or cure UC. Interactions leading to activation of molecular targets are indicated by arrows; those that are inhibited are indicated by a bar.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis

    doi: 10.1155/2016/2601830

    Figure Lengend Snippet: Mechanisms of Kuijie Granule in suppressing ulcerative colitis. Kuijie Granule can improve the clinical symptoms (diarrhea, mucous bloody stool, abdominal pain, abdominal distention, and tenesmus) in UC patients by regulating TGF- β /Smads signal transduction pathway. Kuijie Granule decreased the expression of TGF- β 1, increased the expression of TGF- β RII, Smad4, and Smad7, and has no effect on Smad2 and Smad6 expression; then, the decreasing of the phosphorylation of anti-inflammation signal proteins and the blocking of the expression of inflammation signal proteins finally reduced inflammation reaction to improve or cure UC. Interactions leading to activation of molecular targets are indicated by arrows; those that are inhibited are indicated by a bar.

    Article Snippet: Reagents Rabbit anti-TGF-β 1, rabbit anti-TGF-β RII, rabbit anti-Smad2, rabbit anti-Smad4, rabbit anti-Smad6, and rabbit anti-Smad7 monoclonal antibody were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Transduction, Expressing, Blocking Assay, Activation Assay

    Study flow chart of Kuijie Granule for ulcerative colitis. In this study, we take steps as follows: (1) determine the diagnostic criteria for UC; (2) collect the cases and score the main symptoms; (3) collect the colonic pathological tissue to detect and analyze the expression of TGF- β /Smads, which include TGF- β 1, TGF- β RII, Smad2, Smad4, Smad6, and Smad7; (4) treat UC patients with Kuijie Granule for 60 days; (5) repeat steps (2) and (3); (6) compare the main symptoms scores and TGF- β /Smads expression before and after Kuijie Granule treatment; (7) get the conclusions.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis

    doi: 10.1155/2016/2601830

    Figure Lengend Snippet: Study flow chart of Kuijie Granule for ulcerative colitis. In this study, we take steps as follows: (1) determine the diagnostic criteria for UC; (2) collect the cases and score the main symptoms; (3) collect the colonic pathological tissue to detect and analyze the expression of TGF- β /Smads, which include TGF- β 1, TGF- β RII, Smad2, Smad4, Smad6, and Smad7; (4) treat UC patients with Kuijie Granule for 60 days; (5) repeat steps (2) and (3); (6) compare the main symptoms scores and TGF- β /Smads expression before and after Kuijie Granule treatment; (7) get the conclusions.

    Article Snippet: Reagents Rabbit anti-TGF-β 1, rabbit anti-TGF-β RII, rabbit anti-Smad2, rabbit anti-Smad4, rabbit anti-Smad6, and rabbit anti-Smad7 monoclonal antibody were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Flow Cytometry, Diagnostic Assay, Expressing

    Immunohistochemical staining of liver sections 3 days and 1, 2, 4, 8 and 12 weeks after 20 Gy irradiation. Brown color denotes positivity. The perivenous area was the major area of positive staining. TGF-β1, Smad3, CTGF and NF-κB p65 staining was positive between 3 days and 12 weeks after irradiation. Smad4 and Smad7 staining was positive between 3 days and 1 week after irradiation. TNF-α staining was positive between 1 and 4 weeks after irradiation. (Magnification, ×200). Gy, grays; TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor; TNFα, tumor necrosis factor-α, NF, nuclear factor.

    Journal: Molecular Medicine Reports

    Article Title: Molecular responses of radiation-induced liver damage in rats

    doi: 10.3892/mmr.2014.3051

    Figure Lengend Snippet: Immunohistochemical staining of liver sections 3 days and 1, 2, 4, 8 and 12 weeks after 20 Gy irradiation. Brown color denotes positivity. The perivenous area was the major area of positive staining. TGF-β1, Smad3, CTGF and NF-κB p65 staining was positive between 3 days and 12 weeks after irradiation. Smad4 and Smad7 staining was positive between 3 days and 1 week after irradiation. TNF-α staining was positive between 1 and 4 weeks after irradiation. (Magnification, ×200). Gy, grays; TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor; TNFα, tumor necrosis factor-α, NF, nuclear factor.

    Article Snippet: The primary antibodies used were as follows: Rabbit monoclonal antibodies against transforming growth factor-β1 (TGF-β1; 1:250), nuclear factor (NF)-κB65 (1:100), mothers against decapentaplegic homolog 4 (Smad4) (1:40), Smad3 (1:250), Smad7 (1:30), tumor necrosis factor-α (TNF-α; 1:200) and connective tissue growth factor (CTGF; 1:200), all purchased from Boster Co., Ltd.

    Techniques: Immunohistochemistry, Staining, Irradiation

    Western blot analysis (ratio of the molecules investigated, vs. GAPDH). Similar to the results obtained using the reverse transcription quantitative polymerase chain reaction, the protein expression levels were as follows: NF-κB p65 was increased between 3 days and 12 weeks after irradiation. CTGF and Smad3 were significantly increased between 2 and 12 weeks after irradiation. TGF-β1 was significantly increased between 1 and 12 weeks after irradiation and TNF-α was significantly increased between 3 days and 4 weeks after irradiation. Smad4 was significantly increased between 3 days and 1 week after irradiation. Smad7 was significantly increased 3 days after irradiation and reduced significantly 1 and 2 weeks after irradiation, however, it remained higher than that in the control. From 4 weeks post-irradiation, the protein expression of Smad7 returned to the control level. * P

    Journal: Molecular Medicine Reports

    Article Title: Molecular responses of radiation-induced liver damage in rats

    doi: 10.3892/mmr.2014.3051

    Figure Lengend Snippet: Western blot analysis (ratio of the molecules investigated, vs. GAPDH). Similar to the results obtained using the reverse transcription quantitative polymerase chain reaction, the protein expression levels were as follows: NF-κB p65 was increased between 3 days and 12 weeks after irradiation. CTGF and Smad3 were significantly increased between 2 and 12 weeks after irradiation. TGF-β1 was significantly increased between 1 and 12 weeks after irradiation and TNF-α was significantly increased between 3 days and 4 weeks after irradiation. Smad4 was significantly increased between 3 days and 1 week after irradiation. Smad7 was significantly increased 3 days after irradiation and reduced significantly 1 and 2 weeks after irradiation, however, it remained higher than that in the control. From 4 weeks post-irradiation, the protein expression of Smad7 returned to the control level. * P

    Article Snippet: The primary antibodies used were as follows: Rabbit monoclonal antibodies against transforming growth factor-β1 (TGF-β1; 1:250), nuclear factor (NF)-κB65 (1:100), mothers against decapentaplegic homolog 4 (Smad4) (1:40), Smad3 (1:250), Smad7 (1:30), tumor necrosis factor-α (TNF-α; 1:200) and connective tissue growth factor (CTGF; 1:200), all purchased from Boster Co., Ltd.

    Techniques: Western Blot, Real-time Polymerase Chain Reaction, Expressing, Irradiation

    mRNA expression levels were determined using reverse transcription quantitative polymerase chain reaction (ratio of the molecules investigated, vs. GAPDH). The mRNA expression of NF-κB p65 was upregulated between 3 days and 12 weeks after irradiation. The mRNA expression levels were as follows: CTGF and Smad3 were significantly upregulated between 2 and 12 weeks after irradiation. TGF-β1 was significantly upregulated between 1 and 12 weeks after irradiation. TNF-α was significantly upregulated between 3 days and 4 weeks after irradiation. Smad4 was significantly upregulated 3 days and 1 week after irradiation. Smad7 was significantly upregulated 3 days after irradiation and reduced significantly 1–2 weeks after irradiation, however, the expression levels remained higher than that in the control. From 4 weeks post-irradiation, the mRNA expression of Smad7 returned to the control level. * P

    Journal: Molecular Medicine Reports

    Article Title: Molecular responses of radiation-induced liver damage in rats

    doi: 10.3892/mmr.2014.3051

    Figure Lengend Snippet: mRNA expression levels were determined using reverse transcription quantitative polymerase chain reaction (ratio of the molecules investigated, vs. GAPDH). The mRNA expression of NF-κB p65 was upregulated between 3 days and 12 weeks after irradiation. The mRNA expression levels were as follows: CTGF and Smad3 were significantly upregulated between 2 and 12 weeks after irradiation. TGF-β1 was significantly upregulated between 1 and 12 weeks after irradiation. TNF-α was significantly upregulated between 3 days and 4 weeks after irradiation. Smad4 was significantly upregulated 3 days and 1 week after irradiation. Smad7 was significantly upregulated 3 days after irradiation and reduced significantly 1–2 weeks after irradiation, however, the expression levels remained higher than that in the control. From 4 weeks post-irradiation, the mRNA expression of Smad7 returned to the control level. * P

    Article Snippet: The primary antibodies used were as follows: Rabbit monoclonal antibodies against transforming growth factor-β1 (TGF-β1; 1:250), nuclear factor (NF)-κB65 (1:100), mothers against decapentaplegic homolog 4 (Smad4) (1:40), Smad3 (1:250), Smad7 (1:30), tumor necrosis factor-α (TNF-α; 1:200) and connective tissue growth factor (CTGF; 1:200), all purchased from Boster Co., Ltd.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Irradiation