Journal: Biomedicines

Article Title: Comparative Analysis of Corneal Wound Healing: Differential Molecular Responses in Tears Following PRK, FS-LASIK, and SMILE Procedures

doi: 10.3390/biomedicines12102289

Figure Lengend Snippet: Primer sequences used in RT-qPCR reaction.

Article Snippet: The following antibodies were used for the ELISA test: anti-TGFβ1 (TGFB1 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ2 (TGFB2 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ3 (TGFB3 ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL1B (Human IL-1 beta ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL15 (IL15 ELISA Kit; MyBiosource, San Diego, CA, USA), anti-VEGFA (VEGFA ELISA Kit; MyBiosource, San Diego, CA, USA), anti-IHNB (IHNB ELISA Kit; MyBiosource, San Diego, CA, USA), anti-SLURP1 (SLURP1 ELISA Kit; MyBiosource, San Diego, CA, USA), and anti-GAPDH (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques:

Journal: Biomedicines

Article Title: Comparative Analysis of Corneal Wound Healing: Differential Molecular Responses in Tears Following PRK, FS-LASIK, and SMILE Procedures

doi: 10.3390/biomedicines12102289

Figure Lengend Snippet: Changes in gene expression in tears of patients who underwent PRK, FS-LASIK, and SMILE over 180 days of observation.

Article Snippet: The following antibodies were used for the ELISA test: anti-TGFβ1 (TGFB1 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ2 (TGFB2 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ3 (TGFB3 ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL1B (Human IL-1 beta ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL15 (IL15 ELISA Kit; MyBiosource, San Diego, CA, USA), anti-VEGFA (VEGFA ELISA Kit; MyBiosource, San Diego, CA, USA), anti-IHNB (IHNB ELISA Kit; MyBiosource, San Diego, CA, USA), anti-SLURP1 (SLURP1 ELISA Kit; MyBiosource, San Diego, CA, USA), and anti-GAPDH (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Gene Expression

Journal: Biomedicines

Article Title: Comparative Analysis of Corneal Wound Healing: Differential Molecular Responses in Tears Following PRK, FS-LASIK, and SMILE Procedures

doi: 10.3390/biomedicines12102289

Figure Lengend Snippet: Changes in concentration profiles of analyzed proteins over a 180-day observation period in patients who underwent PRK procedure. TGF-β1–3 , transforming growth factor beta 1–3; IL-1β , interleukin-1 beta; IL-15 , interleukin-15; IHNBA , inhibin beta A chain; VEGFA , vascular endothelial growth factor A; SLURP1 , secreted Ly-6_uPAR-related protein 1.

Article Snippet: The following antibodies were used for the ELISA test: anti-TGFβ1 (TGFB1 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ2 (TGFB2 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ3 (TGFB3 ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL1B (Human IL-1 beta ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL15 (IL15 ELISA Kit; MyBiosource, San Diego, CA, USA), anti-VEGFA (VEGFA ELISA Kit; MyBiosource, San Diego, CA, USA), anti-IHNB (IHNB ELISA Kit; MyBiosource, San Diego, CA, USA), anti-SLURP1 (SLURP1 ELISA Kit; MyBiosource, San Diego, CA, USA), and anti-GAPDH (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Concentration Assay

Journal: Biomedicines

Article Title: Comparative Analysis of Corneal Wound Healing: Differential Molecular Responses in Tears Following PRK, FS-LASIK, and SMILE Procedures

doi: 10.3390/biomedicines12102289

Figure Lengend Snippet: Changes in concentration profiles of analyzed proteins over a 180-day observation period in patients who underwent FS-LASIK procedure. TGF-β1–3 , transforming growth factor beta 1–3; IL-1β , interleukin-1 beta; IL-15 , interleukin-15; IHNBA , inhibin beta A chain; VEGFA , vascular endothelial growth factor A; SLURP1 , secreted Ly-6_uPAR-related protein 1.

Article Snippet: The following antibodies were used for the ELISA test: anti-TGFβ1 (TGFB1 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ2 (TGFB2 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ3 (TGFB3 ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL1B (Human IL-1 beta ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL15 (IL15 ELISA Kit; MyBiosource, San Diego, CA, USA), anti-VEGFA (VEGFA ELISA Kit; MyBiosource, San Diego, CA, USA), anti-IHNB (IHNB ELISA Kit; MyBiosource, San Diego, CA, USA), anti-SLURP1 (SLURP1 ELISA Kit; MyBiosource, San Diego, CA, USA), and anti-GAPDH (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Concentration Assay

Journal: Biomedicines

Article Title: Comparative Analysis of Corneal Wound Healing: Differential Molecular Responses in Tears Following PRK, FS-LASIK, and SMILE Procedures

doi: 10.3390/biomedicines12102289

Figure Lengend Snippet: Changes in concentration profiles of analyzed proteins over a 180-day observation period in patients who underwent SMILE procedure. TGF-β1–3 , transforming growth factor beta 1–3; IL-1β , interleukin-1 beta; IL-15 , interleukin-15; IHNBA , inhibin beta A chain; VEGFA , vascular endothelial growth factor A; SLURP1 , secreted Ly-6_uPAR-related protein 1.

Article Snippet: The following antibodies were used for the ELISA test: anti-TGFβ1 (TGFB1 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ2 (TGFB2 ELISA kit; MyBiosource, San Diego, CA, USA), anti-TGFβ3 (TGFB3 ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL1B (Human IL-1 beta ELISA kit; MyBiosource, San Diego, CA, USA), anti-IL15 (IL15 ELISA Kit; MyBiosource, San Diego, CA, USA), anti-VEGFA (VEGFA ELISA Kit; MyBiosource, San Diego, CA, USA), anti-IHNB (IHNB ELISA Kit; MyBiosource, San Diego, CA, USA), anti-SLURP1 (SLURP1 ELISA Kit; MyBiosource, San Diego, CA, USA), and anti-GAPDH (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Stress Affects Mast Cell Proteases in Murine Skin in a Model of Atopic Dermatitis-like Allergic Inflammation

doi: 10.3390/ijms25115738

Figure Lengend Snippet: SP induces mMCP4 mRNA in the presence of Chrna7 in cultured MCs. ( a , b ) qRTPCR analysis of mMCP4 and mMCP6 mRNA expression in primary cultures of peritoneal MCs from Wt and α7-KO mice. Individual treatments are mentioned at the X axis of the graphs. Gene expressions were normalized to the housekeeping gene TBP. Data represent the averages of at least 3 different experiments. * = p ≤ 0.05. Abbr.: α7-KO = Chrna7 knockout; mMCP = murine mast cell protease; SLURP1 = Ly-6/uPAR-related protein 1; SP = substance P; Wt = wild type.

Article Snippet: SLURP1 (Abnova, Taipei, Taiwan, Cat No. H00057152-P01–10 μg) , 5 ng/mL , Chrna7 agonist.

Techniques: Cell Culture, Expressing, Knock-Out

Journal: International Journal of Molecular Sciences

Article Title: Stress Affects Mast Cell Proteases in Murine Skin in a Model of Atopic Dermatitis-like Allergic Inflammation

doi: 10.3390/ijms25115738

Figure Lengend Snippet: Substances used in PMCC.

Article Snippet: SLURP1 (Abnova, Taipei, Taiwan, Cat No. H00057152-P01–10 μg) , 5 ng/mL , Chrna7 agonist.

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: Endogenous α7 nAChR Agonist SLURP1 Facilitates Escherichia coli K1 Crossing the Blood-Brain Barrier

doi: 10.3389/fimmu.2021.745854

Figure Lengend Snippet: The effect of SLURP1 on E coli K1 penetration and PMN transmigration across the BBB in vitro . (A) The invasion of E coli K1 into HBMEC which pretreated with indicated doses of BSA or SLURP1. Data are presented as percent of the control values. (B) Transwell-cultured HBMEC monolayers were pre treatment with indicated doses of BSA or SLURP1 for 2 h, followed by incubated with E coli K1 in the bottom and PMN in the top of filter successively. PMN in the bottom of filter were harvested and counted. (C) The growth curve of E coli K1 in medium containing indicated doses of SLURP1. (D) The upregulation effect of SLURP1 in HBMEC which had been transfected with pCNDA3.1+-SLURP1. (E) The invasion of E coli K1 into HBMEC which had been transfected with pCNDA3.1+-SLURP1. Data are presented as percent of the control values. (F) Effect of SLURP1 upregulation on E coli K1-induced PMN transmigration across the HBMEC monolayers. Data are presented as percent of the control values. (G) The knockdown effect of SLURP1 in HBMEC transfected with siRNA. (H) The invasion of E coli K1 into HBMEC which had been transfected with siRNA. Data are presented as percent of the control values. (I) Effect of SLURP1 knockdown on E coli K1-induced PMN transmigration across the HBMEC monolayers. Data are presented as percent of the control values. Over., overexpression; Kno., knockdown. The immunoblots results are representative of three independent experiments (D, G) . The data are displayed as the mean ± SEM from three independent experiments (A–I) . * p < 0.05; ** p < 0.01 by one-way ANOVA followed by Bonferroni post-hoc test (A, B, F, I) and Student’s t -test (D, E, G, H) . ns, not significant.

Article Snippet: In brief, predesigned siRNA specific for SLURP1 and nontargeting scrambled siRNA (control) were obtained from Santa Cruz Biotechnology (CA, USA).

Techniques: Transmigration Assay, In Vitro, Control, Cell Culture, Incubation, Transfection, Knockdown, Over Expression, Western Blot