slp773 xhoi re  (Thermo Fisher)


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    Structured Review

    Thermo Fisher slp773 xhoi re
    Slp773 Xhoi Re, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slp773 xhoi re/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    slp773 xhoi re - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Electroporation:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. For overexpression of SplA, E. coli BL21Star (DE3) cells were transformed with pET28a-Spl773 by electroporation according to the manufacturer's instructions (Invitrogen).

    Amplification:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: .. Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).

    Purification:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).

    Sequencing:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: .. Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).

    Expressing:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).

    Polymerase Chain Reaction:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: .. Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).

    Gel Extraction:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).

    Transformation Assay:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. For overexpression of SplA, E. coli BL21Star (DE3) cells were transformed with pET28a-Spl773 by electroporation according to the manufacturer's instructions (Invitrogen).

    Recombinant:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: .. Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).

    Over Expression:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. For overexpression of SplA, E. coli BL21Star (DE3) cells were transformed with pET28a-Spl773 by electroporation according to the manufacturer's instructions (Invitrogen).

    Plasmid Preparation:

    Article Title: Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey Bees
    Article Snippet: Recombinant production of SplA The sequence encoding the S-layer protein SplA of P. larvae 04-309 (ERIC II) was amplified from genomic DNA by PCR with the primer pair Slp773_NcoI-for and Slp773_XhoI-re ( ) (Invitrogen) using Phusion High-Fidelity DNA Polymerase (Fermentas) with annealing at 58°C and extension at 72°C for 80 s. PCR was performed in a thermal cycler My Cycler apparatus (Bio-Rad). .. The spl A amplification product was purified using the GeneJET Gel Extraction Kit (Fermentas), followed by digestion with NcoI/XhoI and insertion into the linearized and dephosphorylated expression vector pET28a(+) (Novagen).