site specific gateway recombination technology  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 83

    Structured Review

    Thermo Fisher site specific gateway recombination technology
    Site Specific Gateway Recombination Technology, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/site specific gateway recombination technology/product/Thermo Fisher
    Average 83 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    site specific gateway recombination technology - by Bioz Stars, 2020-02
    83/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma
    Article Snippet: .. To obtain a lentivirus-based plasmid DNA encoding the GADD34 gene, a fragment encoding full-length GADD34 gene in pCR4blunt-TOPO (Thermo Fisher Scientific) were reinserted in the pLenti-CMVTRE3G puro DEST (w811–1; Addgene) vector using a site-specific recombination technique with a Gateway Cloning Kit (Thermo Fisher Scientific). .. The lentivirus-mediated transformation was performed using rtTA3G-expressing U251 ( ) and selected using blasticidin S and puromycin, and clonal cells were analyzed by immunoblots against GADD34 as described below, after doxycycline (200 ng/mL) treatment for 24 hours.

    Article Title: Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis
    Article Snippet: .. Cloning and plant transformation Gateway technology based on site‐specific recombination was used to generate overexpressing lines of RAP2.3 and RAP2.12 by introducing the open reading frames into the pB7WG2 destination vector through ligation into pENTR 1A DS (Thermo‐Fisher Scientific, Waltham, MA, USA; Karimi et al .). .. The primer pairs Forrap2.3‐ox1 and Revrap2.3‐ox1, and Forrap2.12‐ox1 and Revrap2.12‐ox1 (Table ) were used to amplify the open reading frames of RAP2.3 and RAP2.12 from cDNA generated from leaf RNA.

    Article Title: Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana
    Article Snippet: .. Expression of PdMT2A in Salt-Sensitive Yeast Strain The full-length PdMT2A gene was obtained from the date palm cDNA library and cloned into the yeast expression vector pYES-DEST52 (Thermo Fisher Scientific, Carlsbad, CA, USA), using site-specific Gateway™ recombination technology (Thermo Fisher Scientific) downstream of the galactose-inducible GAL1 promoter ( A). .. The recombinant pYES-DEST52-PdMT2A vector (TY) and the empty pYES-DEST52 vector (EV) were transferred to the salt-sensitive mutant yeast (S. cerevisiae ) strain BYT458 [ ] (kindly provided by Hana Sychrova, Czech Republic), using Yeastmaker™ Yeast Transformation System 2 (Clontech Laboratories, Inc., Mountainview, CA, USA), following the manufacturer’s instructions.

    Selection:

    Article Title: Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis
    Article Snippet: Cloning and plant transformation Gateway technology based on site‐specific recombination was used to generate overexpressing lines of RAP2.3 and RAP2.12 by introducing the open reading frames into the pB7WG2 destination vector through ligation into pENTR 1A DS (Thermo‐Fisher Scientific, Waltham, MA, USA; Karimi et al .). .. Plant selection was carried out based on glufosinate resistance from the pB7WG2 vector.

    Amplification:

    Article Title: Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis 1 and for Proper Control of Flowering in Arabidopsis 1 [C] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] [OA]
    Article Snippet: Amplified cDNA was subcloned into pENTR- d -TOPO (Invitrogen) according to the manufacturer's protocol. .. Next, the cDNA was subcloned into the pMDC107 binary vector ( ) by site-specific recombination with Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's protocol.

    Ligation:

    Article Title: Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis
    Article Snippet: .. Cloning and plant transformation Gateway technology based on site‐specific recombination was used to generate overexpressing lines of RAP2.3 and RAP2.12 by introducing the open reading frames into the pB7WG2 destination vector through ligation into pENTR 1A DS (Thermo‐Fisher Scientific, Waltham, MA, USA; Karimi et al .). .. The primer pairs Forrap2.3‐ox1 and Revrap2.3‐ox1, and Forrap2.12‐ox1 and Revrap2.12‐ox1 (Table ) were used to amplify the open reading frames of RAP2.3 and RAP2.12 from cDNA generated from leaf RNA.

    Mutagenesis:

    Article Title: Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana
    Article Snippet: Expression of PdMT2A in Salt-Sensitive Yeast Strain The full-length PdMT2A gene was obtained from the date palm cDNA library and cloned into the yeast expression vector pYES-DEST52 (Thermo Fisher Scientific, Carlsbad, CA, USA), using site-specific Gateway™ recombination technology (Thermo Fisher Scientific) downstream of the galactose-inducible GAL1 promoter ( A). .. The recombinant pYES-DEST52-PdMT2A vector (TY) and the empty pYES-DEST52 vector (EV) were transferred to the salt-sensitive mutant yeast (S. cerevisiae ) strain BYT458 [ ] (kindly provided by Hana Sychrova, Czech Republic), using Yeastmaker™ Yeast Transformation System 2 (Clontech Laboratories, Inc., Mountainview, CA, USA), following the manufacturer’s instructions.

    Isolation:

    Article Title: Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma
    Article Snippet: To obtain a lentivirus-based plasmid DNA encoding the GADD34 gene, a fragment encoding full-length GADD34 gene in pCR4blunt-TOPO (Thermo Fisher Scientific) were reinserted in the pLenti-CMVTRE3G puro DEST (w811–1; Addgene) vector using a site-specific recombination technique with a Gateway Cloning Kit (Thermo Fisher Scientific). .. U87ΔEGFR-RliFluc cell line was generated from U87ΔEGFR as follows; a fragment encoding RliFluc cDNA (codon-optimized and red-shifted Luciola italica luciferase gene) was isolated by Nhe I/ Xho I enzymatic digestion of pCSCW-RliFluc-ImCherry (a gift from Bakhos A. Tannous, Massachusetts General Hospital, Boston, MA; ref. ), and ligated into the unique Sal I site of pLenti PGK Puro DEST (w529–2; Addgene) after blunt-end treatment.

    cDNA Library Assay:

    Article Title: Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana
    Article Snippet: .. Expression of PdMT2A in Salt-Sensitive Yeast Strain The full-length PdMT2A gene was obtained from the date palm cDNA library and cloned into the yeast expression vector pYES-DEST52 (Thermo Fisher Scientific, Carlsbad, CA, USA), using site-specific Gateway™ recombination technology (Thermo Fisher Scientific) downstream of the galactose-inducible GAL1 promoter ( A). .. The recombinant pYES-DEST52-PdMT2A vector (TY) and the empty pYES-DEST52 vector (EV) were transferred to the salt-sensitive mutant yeast (S. cerevisiae ) strain BYT458 [ ] (kindly provided by Hana Sychrova, Czech Republic), using Yeastmaker™ Yeast Transformation System 2 (Clontech Laboratories, Inc., Mountainview, CA, USA), following the manufacturer’s instructions.

    Generated:

    Article Title: Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma
    Article Snippet: To obtain a lentivirus-based plasmid DNA encoding the GADD34 gene, a fragment encoding full-length GADD34 gene in pCR4blunt-TOPO (Thermo Fisher Scientific) were reinserted in the pLenti-CMVTRE3G puro DEST (w811–1; Addgene) vector using a site-specific recombination technique with a Gateway Cloning Kit (Thermo Fisher Scientific). .. U87ΔEGFR-RliFluc cell line was generated from U87ΔEGFR as follows; a fragment encoding RliFluc cDNA (codon-optimized and red-shifted Luciola italica luciferase gene) was isolated by Nhe I/ Xho I enzymatic digestion of pCSCW-RliFluc-ImCherry (a gift from Bakhos A. Tannous, Massachusetts General Hospital, Boston, MA; ref. ), and ligated into the unique Sal I site of pLenti PGK Puro DEST (w529–2; Addgene) after blunt-end treatment.

    Article Title: Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis
    Article Snippet: Cloning and plant transformation Gateway technology based on site‐specific recombination was used to generate overexpressing lines of RAP2.3 and RAP2.12 by introducing the open reading frames into the pB7WG2 destination vector through ligation into pENTR 1A DS (Thermo‐Fisher Scientific, Waltham, MA, USA; Karimi et al .). .. The primer pairs Forrap2.3‐ox1 and Revrap2.3‐ox1, and Forrap2.12‐ox1 and Revrap2.12‐ox1 (Table ) were used to amplify the open reading frames of RAP2.3 and RAP2.12 from cDNA generated from leaf RNA.

    Luciferase:

    Article Title: Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma
    Article Snippet: To obtain a lentivirus-based plasmid DNA encoding the GADD34 gene, a fragment encoding full-length GADD34 gene in pCR4blunt-TOPO (Thermo Fisher Scientific) were reinserted in the pLenti-CMVTRE3G puro DEST (w811–1; Addgene) vector using a site-specific recombination technique with a Gateway Cloning Kit (Thermo Fisher Scientific). .. U87ΔEGFR-RliFluc cell line was generated from U87ΔEGFR as follows; a fragment encoding RliFluc cDNA (codon-optimized and red-shifted Luciola italica luciferase gene) was isolated by Nhe I/ Xho I enzymatic digestion of pCSCW-RliFluc-ImCherry (a gift from Bakhos A. Tannous, Massachusetts General Hospital, Boston, MA; ref. ), and ligated into the unique Sal I site of pLenti PGK Puro DEST (w529–2; Addgene) after blunt-end treatment.

    Expressing:

    Article Title: Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis
    Article Snippet: Cloning and plant transformation Gateway technology based on site‐specific recombination was used to generate overexpressing lines of RAP2.3 and RAP2.12 by introducing the open reading frames into the pB7WG2 destination vector through ligation into pENTR 1A DS (Thermo‐Fisher Scientific, Waltham, MA, USA; Karimi et al .). .. The pB7WG2 expression clones were transformed into Agrobacterium tumefaciens strain GV3101, followed by plant transformation of Col‐0 with the floral dip method (Clough & Bent ).

    Article Title: Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana
    Article Snippet: .. Expression of PdMT2A in Salt-Sensitive Yeast Strain The full-length PdMT2A gene was obtained from the date palm cDNA library and cloned into the yeast expression vector pYES-DEST52 (Thermo Fisher Scientific, Carlsbad, CA, USA), using site-specific Gateway™ recombination technology (Thermo Fisher Scientific) downstream of the galactose-inducible GAL1 promoter ( A). .. The recombinant pYES-DEST52-PdMT2A vector (TY) and the empty pYES-DEST52 vector (EV) were transferred to the salt-sensitive mutant yeast (S. cerevisiae ) strain BYT458 [ ] (kindly provided by Hana Sychrova, Czech Republic), using Yeastmaker™ Yeast Transformation System 2 (Clontech Laboratories, Inc., Mountainview, CA, USA), following the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis 1 and for Proper Control of Flowering in Arabidopsis 1 [C] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] [OA]
    Article Snippet: The UBP26 genomic clone and its 2.8-kb native promoter were amplified by PCR with Phusion High-Fidelity DNA polymerase (Finnzymes) according to the manufacturer's protocol (the termination codon was not included in the amplification). .. Next, the cDNA was subcloned into the pMDC107 binary vector ( ) by site-specific recombination with Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's protocol.

    Western Blot:

    Article Title: Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma
    Article Snippet: To obtain a lentivirus-based plasmid DNA encoding the GADD34 gene, a fragment encoding full-length GADD34 gene in pCR4blunt-TOPO (Thermo Fisher Scientific) were reinserted in the pLenti-CMVTRE3G puro DEST (w811–1; Addgene) vector using a site-specific recombination technique with a Gateway Cloning Kit (Thermo Fisher Scientific). .. The lentivirus-mediated transformation was performed using rtTA3G-expressing U251 ( ) and selected using blasticidin S and puromycin, and clonal cells were analyzed by immunoblots against GADD34 as described below, after doxycycline (200 ng/mL) treatment for 24 hours.

    Transformation Assay:

    Article Title: Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma
    Article Snippet: To obtain a lentivirus-based plasmid DNA encoding the GADD34 gene, a fragment encoding full-length GADD34 gene in pCR4blunt-TOPO (Thermo Fisher Scientific) were reinserted in the pLenti-CMVTRE3G puro DEST (w811–1; Addgene) vector using a site-specific recombination technique with a Gateway Cloning Kit (Thermo Fisher Scientific). .. The lentivirus-mediated transformation was performed using rtTA3G-expressing U251 ( ) and selected using blasticidin S and puromycin, and clonal cells were analyzed by immunoblots against GADD34 as described below, after doxycycline (200 ng/mL) treatment for 24 hours.

    Article Title: Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis
    Article Snippet: .. Cloning and plant transformation Gateway technology based on site‐specific recombination was used to generate overexpressing lines of RAP2.3 and RAP2.12 by introducing the open reading frames into the pB7WG2 destination vector through ligation into pENTR 1A DS (Thermo‐Fisher Scientific, Waltham, MA, USA; Karimi et al .). .. The primer pairs Forrap2.3‐ox1 and Revrap2.3‐ox1, and Forrap2.12‐ox1 and Revrap2.12‐ox1 (Table ) were used to amplify the open reading frames of RAP2.3 and RAP2.12 from cDNA generated from leaf RNA.

    Article Title: Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana
    Article Snippet: Expression of PdMT2A in Salt-Sensitive Yeast Strain The full-length PdMT2A gene was obtained from the date palm cDNA library and cloned into the yeast expression vector pYES-DEST52 (Thermo Fisher Scientific, Carlsbad, CA, USA), using site-specific Gateway™ recombination technology (Thermo Fisher Scientific) downstream of the galactose-inducible GAL1 promoter ( A). .. The recombinant pYES-DEST52-PdMT2A vector (TY) and the empty pYES-DEST52 vector (EV) were transferred to the salt-sensitive mutant yeast (S. cerevisiae ) strain BYT458 [ ] (kindly provided by Hana Sychrova, Czech Republic), using Yeastmaker™ Yeast Transformation System 2 (Clontech Laboratories, Inc., Mountainview, CA, USA), following the manufacturer’s instructions.

    Article Title: Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis 1 and for Proper Control of Flowering in Arabidopsis 1 [C] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] [OA]
    Article Snippet: Next, the cDNA was subcloned into the pMDC107 binary vector ( ) by site-specific recombination with Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's protocol. .. Plasmids were transformed into agrobacterium, which was used for transformation of C24 and ubp26 - 1 .

    Recombinant:

    Article Title: Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana
    Article Snippet: Expression of PdMT2A in Salt-Sensitive Yeast Strain The full-length PdMT2A gene was obtained from the date palm cDNA library and cloned into the yeast expression vector pYES-DEST52 (Thermo Fisher Scientific, Carlsbad, CA, USA), using site-specific Gateway™ recombination technology (Thermo Fisher Scientific) downstream of the galactose-inducible GAL1 promoter ( A). .. The recombinant pYES-DEST52-PdMT2A vector (TY) and the empty pYES-DEST52 vector (EV) were transferred to the salt-sensitive mutant yeast (S. cerevisiae ) strain BYT458 [ ] (kindly provided by Hana Sychrova, Czech Republic), using Yeastmaker™ Yeast Transformation System 2 (Clontech Laboratories, Inc., Mountainview, CA, USA), following the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma
    Article Snippet: .. To obtain a lentivirus-based plasmid DNA encoding the GADD34 gene, a fragment encoding full-length GADD34 gene in pCR4blunt-TOPO (Thermo Fisher Scientific) were reinserted in the pLenti-CMVTRE3G puro DEST (w811–1; Addgene) vector using a site-specific recombination technique with a Gateway Cloning Kit (Thermo Fisher Scientific). .. The lentivirus-mediated transformation was performed using rtTA3G-expressing U251 ( ) and selected using blasticidin S and puromycin, and clonal cells were analyzed by immunoblots against GADD34 as described below, after doxycycline (200 ng/mL) treatment for 24 hours.

    Article Title: Hypoxia and the group VII ethylene response transcription factor HRE2 promote adventitious root elongation in Arabidopsis
    Article Snippet: .. Cloning and plant transformation Gateway technology based on site‐specific recombination was used to generate overexpressing lines of RAP2.3 and RAP2.12 by introducing the open reading frames into the pB7WG2 destination vector through ligation into pENTR 1A DS (Thermo‐Fisher Scientific, Waltham, MA, USA; Karimi et al .). .. The primer pairs Forrap2.3‐ox1 and Revrap2.3‐ox1, and Forrap2.12‐ox1 and Revrap2.12‐ox1 (Table ) were used to amplify the open reading frames of RAP2.3 and RAP2.12 from cDNA generated from leaf RNA.

    Article Title: Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana
    Article Snippet: .. Expression of PdMT2A in Salt-Sensitive Yeast Strain The full-length PdMT2A gene was obtained from the date palm cDNA library and cloned into the yeast expression vector pYES-DEST52 (Thermo Fisher Scientific, Carlsbad, CA, USA), using site-specific Gateway™ recombination technology (Thermo Fisher Scientific) downstream of the galactose-inducible GAL1 promoter ( A). .. The recombinant pYES-DEST52-PdMT2A vector (TY) and the empty pYES-DEST52 vector (EV) were transferred to the salt-sensitive mutant yeast (S. cerevisiae ) strain BYT458 [ ] (kindly provided by Hana Sychrova, Czech Republic), using Yeastmaker™ Yeast Transformation System 2 (Clontech Laboratories, Inc., Mountainview, CA, USA), following the manufacturer’s instructions.

    Article Title: Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis 1 and for Proper Control of Flowering in Arabidopsis 1 [C] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] and for Proper Control of Flowering in Arabidopsis 1 [C] [W] [OA]
    Article Snippet: .. Next, the cDNA was subcloned into the pMDC107 binary vector ( ) by site-specific recombination with Gateway LR Clonase II enzyme mix (Invitrogen) according to the manufacturer's protocol. .. Plasmids were transformed into agrobacterium, which was used for transformation of C24 and ubp26 - 1 .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 83
    Thermo Fisher site specific gateway recombination technology
    Site Specific Gateway Recombination Technology, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/site specific gateway recombination technology/product/Thermo Fisher
    Average 83 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    site specific gateway recombination technology - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    83
    Thermo Fisher site specific recombination technique
    Site Specific Recombination Technique, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/site specific recombination technique/product/Thermo Fisher
    Average 83 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    site specific recombination technique - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    Image Search Results