sirna transfections hearts  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical sirna transfections hearts
    Sirna Transfections Hearts, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna transfections hearts/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sirna transfections hearts - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Cell Culture:

    Article Title: AMPK regulates cell shape of cardiomyocytes by modulating turnover of microtubules through CLIP-170
    Article Snippet: Specific reactivity with the targeted phosphoserine sequence was confirmed by an ELISA in which phosphorylated or non-phosphorylated peptides were coated. .. Cell culture, plasmid transfection, and siRNA Primary cultures of neonatal cardiomyocytes were prepared from 1- to 3-day-old Wistar rats as described previously [ , ]. .. Briefly, harvested hearts were incubated in 0.25% trypsin/EDTA (Sigma) at 4°C overnight and then digested with collagenase type II (Worthington).

    Article Title: AMPK regulates cell shape of cardiomyocytes by modulating turnover of microtubules through CLIP‐170
    Article Snippet: Specific reactivity with the targeted phosphoserine sequence was confirmed by an ELISA in which phosphorylated or non‐phosphorylated peptides were coated. .. Cell culture, plasmid transfection, and siRNA Primary cultures of neonatal cardiomyocytes were prepared from 1‐ to 3‐day‐old Wistar rats as described previously (Seguchi et al , ; Shintani et al , ). .. Briefly, harvested hearts were incubated in 0.25% trypsin/EDTA (Sigma) at 4°C overnight and then digested with collagenase type II (Worthington).

    Article Title: DNA single-strand break-induced DNA damage response causes heart failure
    Article Snippet: .. Cell culture and siRNA transfections Hearts were excised from newborn (1- to 2-day-old) Sprague-Dawley rats and neonatal rat cardiomyocytes (NRCMs) were isolated using Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp.) according to the manufacturer's instructions . .. NRCMs were cultured in DMEM (Wako) supplemented with 10% fetal bovine serum, 10 μM cytosine β-D -arabinofuranoside hydrochloride (Ara-C, Sigma).

    Plasmid Preparation:

    Article Title: AMPK regulates cell shape of cardiomyocytes by modulating turnover of microtubules through CLIP-170
    Article Snippet: Specific reactivity with the targeted phosphoserine sequence was confirmed by an ELISA in which phosphorylated or non-phosphorylated peptides were coated. .. Cell culture, plasmid transfection, and siRNA Primary cultures of neonatal cardiomyocytes were prepared from 1- to 3-day-old Wistar rats as described previously [ , ]. .. Briefly, harvested hearts were incubated in 0.25% trypsin/EDTA (Sigma) at 4°C overnight and then digested with collagenase type II (Worthington).

    Article Title: AMPK regulates cell shape of cardiomyocytes by modulating turnover of microtubules through CLIP‐170
    Article Snippet: Specific reactivity with the targeted phosphoserine sequence was confirmed by an ELISA in which phosphorylated or non‐phosphorylated peptides were coated. .. Cell culture, plasmid transfection, and siRNA Primary cultures of neonatal cardiomyocytes were prepared from 1‐ to 3‐day‐old Wistar rats as described previously (Seguchi et al , ; Shintani et al , ). .. Briefly, harvested hearts were incubated in 0.25% trypsin/EDTA (Sigma) at 4°C overnight and then digested with collagenase type II (Worthington).

    Transfection:

    Article Title: AMPK regulates cell shape of cardiomyocytes by modulating turnover of microtubules through CLIP-170
    Article Snippet: Specific reactivity with the targeted phosphoserine sequence was confirmed by an ELISA in which phosphorylated or non-phosphorylated peptides were coated. .. Cell culture, plasmid transfection, and siRNA Primary cultures of neonatal cardiomyocytes were prepared from 1- to 3-day-old Wistar rats as described previously [ , ]. .. Briefly, harvested hearts were incubated in 0.25% trypsin/EDTA (Sigma) at 4°C overnight and then digested with collagenase type II (Worthington).

    Article Title: AMPK regulates cell shape of cardiomyocytes by modulating turnover of microtubules through CLIP‐170
    Article Snippet: Specific reactivity with the targeted phosphoserine sequence was confirmed by an ELISA in which phosphorylated or non‐phosphorylated peptides were coated. .. Cell culture, plasmid transfection, and siRNA Primary cultures of neonatal cardiomyocytes were prepared from 1‐ to 3‐day‐old Wistar rats as described previously (Seguchi et al , ; Shintani et al , ). .. Briefly, harvested hearts were incubated in 0.25% trypsin/EDTA (Sigma) at 4°C overnight and then digested with collagenase type II (Worthington).

    Article Title: DNA single-strand break-induced DNA damage response causes heart failure
    Article Snippet: .. Cell culture and siRNA transfections Hearts were excised from newborn (1- to 2-day-old) Sprague-Dawley rats and neonatal rat cardiomyocytes (NRCMs) were isolated using Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp.) according to the manufacturer's instructions . .. NRCMs were cultured in DMEM (Wako) supplemented with 10% fetal bovine serum, 10 μM cytosine β-D -arabinofuranoside hydrochloride (Ara-C, Sigma).

    Isolation:

    Article Title: Tsg101 positively regulates P62-Keap1-Nrf2 pathway to protect hearts against oxidative damage
    Article Snippet: The adult animal cardiomyocytes were cultured on laminin (10 μg/ml, Life technologies)-coated plates with AW medium (Cellutron life technologies) with 10% FBS, followed by treatment with H2 O2 (200 μM) for 1 h. Then, cell survival was determined using a MTS analysis kit (Promega). .. 2.8 Isolation of neonatal animal cardiomyocytes and infection/transfection with recombinant adenoviruses and siRNAs Neonatal cardiomyocytes were isolated from ventricles of 1–3 day old neonatal Sprague Dawley rats, or mice (FVB/N, 1–3 day old) using a neonatal cardiomyocyte isolation kit (Worthington Biochemical, LK003300). .. Per the manufacturer's instructions, cell pellets were obtained after enzyme digestion and centrifugation.

    Article Title: DNA single-strand break-induced DNA damage response causes heart failure
    Article Snippet: .. Cell culture and siRNA transfections Hearts were excised from newborn (1- to 2-day-old) Sprague-Dawley rats and neonatal rat cardiomyocytes (NRCMs) were isolated using Neonatal Cardiomyocyte Isolation System (Worthington Biochemical Corp.) according to the manufacturer's instructions . .. NRCMs were cultured in DMEM (Wako) supplemented with 10% fetal bovine serum, 10 μM cytosine β-D -arabinofuranoside hydrochloride (Ara-C, Sigma).

    Infection:

    Article Title: Tsg101 positively regulates P62-Keap1-Nrf2 pathway to protect hearts against oxidative damage
    Article Snippet: The adult animal cardiomyocytes were cultured on laminin (10 μg/ml, Life technologies)-coated plates with AW medium (Cellutron life technologies) with 10% FBS, followed by treatment with H2 O2 (200 μM) for 1 h. Then, cell survival was determined using a MTS analysis kit (Promega). .. 2.8 Isolation of neonatal animal cardiomyocytes and infection/transfection with recombinant adenoviruses and siRNAs Neonatal cardiomyocytes were isolated from ventricles of 1–3 day old neonatal Sprague Dawley rats, or mice (FVB/N, 1–3 day old) using a neonatal cardiomyocyte isolation kit (Worthington Biochemical, LK003300). .. Per the manufacturer's instructions, cell pellets were obtained after enzyme digestion and centrifugation.

    Recombinant:

    Article Title: Tsg101 positively regulates P62-Keap1-Nrf2 pathway to protect hearts against oxidative damage
    Article Snippet: The adult animal cardiomyocytes were cultured on laminin (10 μg/ml, Life technologies)-coated plates with AW medium (Cellutron life technologies) with 10% FBS, followed by treatment with H2 O2 (200 μM) for 1 h. Then, cell survival was determined using a MTS analysis kit (Promega). .. 2.8 Isolation of neonatal animal cardiomyocytes and infection/transfection with recombinant adenoviruses and siRNAs Neonatal cardiomyocytes were isolated from ventricles of 1–3 day old neonatal Sprague Dawley rats, or mice (FVB/N, 1–3 day old) using a neonatal cardiomyocyte isolation kit (Worthington Biochemical, LK003300). .. Per the manufacturer's instructions, cell pellets were obtained after enzyme digestion and centrifugation.

    Mouse Assay:

    Article Title: Tsg101 positively regulates P62-Keap1-Nrf2 pathway to protect hearts against oxidative damage
    Article Snippet: The adult animal cardiomyocytes were cultured on laminin (10 μg/ml, Life technologies)-coated plates with AW medium (Cellutron life technologies) with 10% FBS, followed by treatment with H2 O2 (200 μM) for 1 h. Then, cell survival was determined using a MTS analysis kit (Promega). .. 2.8 Isolation of neonatal animal cardiomyocytes and infection/transfection with recombinant adenoviruses and siRNAs Neonatal cardiomyocytes were isolated from ventricles of 1–3 day old neonatal Sprague Dawley rats, or mice (FVB/N, 1–3 day old) using a neonatal cardiomyocyte isolation kit (Worthington Biochemical, LK003300). .. Per the manufacturer's instructions, cell pellets were obtained after enzyme digestion and centrifugation.

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