sirna shnr2e3  (Millipore)


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    Name:
    MISSION shRNA
    Description:
    Our comprehensive shRNA product offering consists of over 150 000 pre cloned shRNA constructs targeting more than 15 000 human and 15 000 mouse genes The library is available in gene family sets gene collections related to specific functional classes such as kinases and ion channels gene taget sets sets of shRNA clones that target your favorite gene and individual shRNA clones Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state of the art robotics liquid handling and laboratory information management systems LIMS to provide superior quality and service for your RNAi research needs
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    cstvrs
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    Structured Review

    Millipore sirna shnr2e3
    NR2E3 regulates ESR1 function in breast cancer cells A-B. MCF-7 cells were stably transfected with <t>shNR2E3</t> or control shRNA (shCon). Total RNA from indicated cell extracted and analysed by qRT-PCR with indicated probe. C-D. T47D cells were stably transfected with shNR2E3 or shCon. Total RNA and protein from indicated cell extracts were analysed by qRT-PCR with indicated probes. E. MCF-7 cells were stably transfected with shNR2E3 or control shRNA (shCon). Total protein from indicated cell extracted and analysed by Western blot with indicated antibody. F. Stably transfected MCF-7 with shNR2E3 or with shCon were transfected with ESR1 promoter construct, and the cells were harvested for luciferase assay. Values indicated relatively normalized luciferase activity. G-H. MCF-7 cells were transiently transfected with indicated <t>siRNA,</t> and cell lysates were used for Western blot ( G ) or for qRT-PCR ( H ). All results are shown as mean plus standard deviation (SD) from three-independent replicates (* p
    Our comprehensive shRNA product offering consists of over 150 000 pre cloned shRNA constructs targeting more than 15 000 human and 15 000 mouse genes The library is available in gene family sets gene collections related to specific functional classes such as kinases and ion channels gene taget sets sets of shRNA clones that target your favorite gene and individual shRNA clones Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state of the art robotics liquid handling and laboratory information management systems LIMS to provide superior quality and service for your RNAi research needs
    https://www.bioz.com/result/sirna shnr2e3/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    sirna shnr2e3 - by Bioz Stars, 2020-09
    99/100 stars

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    Images

    1) Product Images from "Reconstruction of nuclear receptor network reveals that NR2E3 is a novel upstream regulator of ESR1 in breast cancer"

    Article Title: Reconstruction of nuclear receptor network reveals that NR2E3 is a novel upstream regulator of ESR1 in breast cancer

    Journal: EMBO Molecular Medicine

    doi: 10.1002/emmm.201100187

    NR2E3 regulates ESR1 function in breast cancer cells A-B. MCF-7 cells were stably transfected with shNR2E3 or control shRNA (shCon). Total RNA from indicated cell extracted and analysed by qRT-PCR with indicated probe. C-D. T47D cells were stably transfected with shNR2E3 or shCon. Total RNA and protein from indicated cell extracts were analysed by qRT-PCR with indicated probes. E. MCF-7 cells were stably transfected with shNR2E3 or control shRNA (shCon). Total protein from indicated cell extracted and analysed by Western blot with indicated antibody. F. Stably transfected MCF-7 with shNR2E3 or with shCon were transfected with ESR1 promoter construct, and the cells were harvested for luciferase assay. Values indicated relatively normalized luciferase activity. G-H. MCF-7 cells were transiently transfected with indicated siRNA, and cell lysates were used for Western blot ( G ) or for qRT-PCR ( H ). All results are shown as mean plus standard deviation (SD) from three-independent replicates (* p
    Figure Legend Snippet: NR2E3 regulates ESR1 function in breast cancer cells A-B. MCF-7 cells were stably transfected with shNR2E3 or control shRNA (shCon). Total RNA from indicated cell extracted and analysed by qRT-PCR with indicated probe. C-D. T47D cells were stably transfected with shNR2E3 or shCon. Total RNA and protein from indicated cell extracts were analysed by qRT-PCR with indicated probes. E. MCF-7 cells were stably transfected with shNR2E3 or control shRNA (shCon). Total protein from indicated cell extracted and analysed by Western blot with indicated antibody. F. Stably transfected MCF-7 with shNR2E3 or with shCon were transfected with ESR1 promoter construct, and the cells were harvested for luciferase assay. Values indicated relatively normalized luciferase activity. G-H. MCF-7 cells were transiently transfected with indicated siRNA, and cell lysates were used for Western blot ( G ) or for qRT-PCR ( H ). All results are shown as mean plus standard deviation (SD) from three-independent replicates (* p

    Techniques Used: Stable Transfection, Transfection, shRNA, Quantitative RT-PCR, Western Blot, Construct, Luciferase, Activity Assay, Standard Deviation

    Related Articles

    Transduction:

    Article Title: CAPER as a therapeutic target for triple negative breast cancer
    Article Snippet: .. Transduction-ready control shRNA (cat# SHC001V) and human CAPER shRNA lentiviral particles (cat# SHCLNV-NM_004902 (clone# TRCN0000021770 and TRCN0000021769 abbreviated as shRNA #70 and #69)) were purchased from Sigma-Aldrich (St. Louis, MO). .. Rabbit polyclonal anti-CAPER antibody (cat# 3733-100) was purchased from BioVision Inc (Milpitas, CA).

    Article Title: SMAC mimetics induce autophagy-dependent apoptosis of HIV-1-infected resting memory CD4+ T cells
    Article Snippet: .. Lentiviral transduction with MISSION lentiviral particles containing short hairpin RNA (shRNA) targeting ATG2A (SHCLNV- /TRCN0000168420), ATG2B (SHCLNV- /TRCN0000150114), ATG5 (SHCLNV- /TRCN0000151963), ATG7 (SHCLNV- /TRCN0000007584), RUBCN (SHCLNV- /TRCN0000235638), SQSTM1 (SHCLNV- /TRCN0000007237), ULK1 (SHCLNV- /TRCN0000000835), or scrambled non-target negative control (SHC002V) was performed according to the manufacturer's protocol (Sigma). .. SHC002V was used as non-targeting negative control as it activates the RNA-induced silencing complex and the RNAi pathway, but does not target any human gene allowing the examination of the effects of shRNA transduction and RNAi activation on gene expression.

    Article Title: RIPK1 regulates survival of human melanoma cells upon endoplasmic reticulum stress through autophagy
    Article Snippet: .. Short hairpin RNA (shRNA) knockdown Sigma-Aldrich MISSION® Lentiviral Transduction Particles for shRNA-mediated knockdown of ERN1 (SHCLNV-NM_001433), ATF6 (SHCLNV-NM_007348) and EIF2AK3 (SHCLNV-NM_032025) were purchased from Sigma-Aldrich and used as described previously. .. The human RIPK1 shRNA (TG320591), BCL2L11 shRNA (TG306422), BECN1 shRNA (TG314484) and scrambled shRNA (TG300130), were purchased from Origene, and were used to infect cells according to the manufacturer's protocol.

    Transfection:

    Article Title: GPR119 agonist enhances gefitinib responsiveness through lactate-mediated inhibition of autophagy
    Article Snippet: .. MCF-7 cells and HepG2 cells were transfected with siRNA using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). shRNA lentivirus particles for GPR119 (cat # SHCLNV-NM_178471) and Nontarget control (cat # SHV0002) were purchased from Sigma-Aldrich. .. MCF-7 cell was incubated with lentivirus particles and hexamidine bromide.

    shRNA:

    Article Title: Reconstruction of nuclear receptor network reveals that NR2E3 is a novel upstream regulator of ESR1 in breast cancer
    Article Snippet: .. shRNA and siRNA shNR2E3 (SHCLND-NM_014249) and shControl (SHC002) clones were purchased from Sigma (St. Louis, MO). .. To express shRNA, we transfected shRNA expression vectors into the cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA).

    Article Title: CAPER as a therapeutic target for triple negative breast cancer
    Article Snippet: .. Transduction-ready control shRNA (cat# SHC001V) and human CAPER shRNA lentiviral particles (cat# SHCLNV-NM_004902 (clone# TRCN0000021770 and TRCN0000021769 abbreviated as shRNA #70 and #69)) were purchased from Sigma-Aldrich (St. Louis, MO). .. Rabbit polyclonal anti-CAPER antibody (cat# 3733-100) was purchased from BioVision Inc (Milpitas, CA).

    Article Title: GPR119 agonist enhances gefitinib responsiveness through lactate-mediated inhibition of autophagy
    Article Snippet: .. MCF-7 cells and HepG2 cells were transfected with siRNA using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). shRNA lentivirus particles for GPR119 (cat # SHCLNV-NM_178471) and Nontarget control (cat # SHV0002) were purchased from Sigma-Aldrich. .. MCF-7 cell was incubated with lentivirus particles and hexamidine bromide.

    Article Title: The signaling axis atypical protein kinase C λ/ι-Satb2 mediates leukemic transformation of B-cell progenitors
    Article Snippet: .. The lentiviral vectors for aPKCι shRNA (pLKO.1-CMV-tGFP shRNA plasmid DNA, catalog SHCLND 10141531MN) and control shRNA (pLKO.1-puro-CMV-TurboGFP+ control plasmid DNA, catalog SHC003 02101412MN) were obtained from Sigma-Aldrich. .. High titter lentiviruses of Satb2, Etv5 and mock vector were generated in 293 T cells.

    Article Title: SMAC mimetics induce autophagy-dependent apoptosis of HIV-1-infected resting memory CD4+ T cells
    Article Snippet: .. Lentiviral transduction with MISSION lentiviral particles containing short hairpin RNA (shRNA) targeting ATG2A (SHCLNV- /TRCN0000168420), ATG2B (SHCLNV- /TRCN0000150114), ATG5 (SHCLNV- /TRCN0000151963), ATG7 (SHCLNV- /TRCN0000007584), RUBCN (SHCLNV- /TRCN0000235638), SQSTM1 (SHCLNV- /TRCN0000007237), ULK1 (SHCLNV- /TRCN0000000835), or scrambled non-target negative control (SHC002V) was performed according to the manufacturer's protocol (Sigma). .. SHC002V was used as non-targeting negative control as it activates the RNA-induced silencing complex and the RNAi pathway, but does not target any human gene allowing the examination of the effects of shRNA transduction and RNAi activation on gene expression.

    Article Title: RIPK1 regulates survival of human melanoma cells upon endoplasmic reticulum stress through autophagy
    Article Snippet: .. Short hairpin RNA (shRNA) knockdown Sigma-Aldrich MISSION® Lentiviral Transduction Particles for shRNA-mediated knockdown of ERN1 (SHCLNV-NM_001433), ATF6 (SHCLNV-NM_007348) and EIF2AK3 (SHCLNV-NM_032025) were purchased from Sigma-Aldrich and used as described previously. .. The human RIPK1 shRNA (TG320591), BCL2L11 shRNA (TG306422), BECN1 shRNA (TG314484) and scrambled shRNA (TG300130), were purchased from Origene, and were used to infect cells according to the manufacturer's protocol.

    Negative Control:

    Article Title: SMAC mimetics induce autophagy-dependent apoptosis of HIV-1-infected resting memory CD4+ T cells
    Article Snippet: .. Lentiviral transduction with MISSION lentiviral particles containing short hairpin RNA (shRNA) targeting ATG2A (SHCLNV- /TRCN0000168420), ATG2B (SHCLNV- /TRCN0000150114), ATG5 (SHCLNV- /TRCN0000151963), ATG7 (SHCLNV- /TRCN0000007584), RUBCN (SHCLNV- /TRCN0000235638), SQSTM1 (SHCLNV- /TRCN0000007237), ULK1 (SHCLNV- /TRCN0000000835), or scrambled non-target negative control (SHC002V) was performed according to the manufacturer's protocol (Sigma). .. SHC002V was used as non-targeting negative control as it activates the RNA-induced silencing complex and the RNAi pathway, but does not target any human gene allowing the examination of the effects of shRNA transduction and RNAi activation on gene expression.

    Produced:

    Article Title: Arsenite Suppression of BMP Signaling in Human Keratinocytes
    Article Snippet: .. Knockdowns of FOXN1, DUSP2 and DUSP14 in SIK cells were produced using Mission shRNAs (Sigma-Aldrich, St. Louis, MO). .. Viral particles were prepared in HEK293FT cells by co-transfection of the Mission shRNA with ViraPower Packaging Mix using Lipofectamine 2000 transfection reagent, both from Life Technologies (Carlsbad, CA).

    Plasmid Preparation:

    Article Title: The signaling axis atypical protein kinase C λ/ι-Satb2 mediates leukemic transformation of B-cell progenitors
    Article Snippet: .. The lentiviral vectors for aPKCι shRNA (pLKO.1-CMV-tGFP shRNA plasmid DNA, catalog SHCLND 10141531MN) and control shRNA (pLKO.1-puro-CMV-TurboGFP+ control plasmid DNA, catalog SHC003 02101412MN) were obtained from Sigma-Aldrich. .. High titter lentiviruses of Satb2, Etv5 and mock vector were generated in 293 T cells.