Journal: iScience

Article Title: Origin and development of uniparental and polyploid blastomeres

doi: 10.1016/j.isci.2025.112337

Figure Lengend Snippet: Blastomeres with whole-genome abnormalities can reach blastocyst stage despite impaired developmental potential (A) Distribution of 53 biparental diploid blastomere outgrowths and 48 blastomere outgrowths with WG abnormalities across different time points of outgrowth collection, with the x axis representing the fraction of counts. (B) Developmental stage comparison of biparental diploid blastomere outgrowths and blastomeres outgrowths with WG abnormalities in (A), with the x axis representing the fraction of counts (Fisher’s exact test). (C) Schematic representation of the six blastomere outgrowths with WG abnormalities that reached the blastocyst stage at T3, along with their sibling blastomere outgrowths and original embryos. (D) Images of the two cleaved anuclear blastomere outgrowths and schematic diagrams illustrating the embryos from which they originated. In (C) and (D), the same symbols as in Figure 2 C are employed. Fertilization and the first cleavage are not depicted for embryos containing blastomeres with an unknown haplotype (due to sample loss or haplotype failure). See also Figure S1 and Tables S1 , , and .

Article Snippet: For a subset of blastocyst stage single cells, the libraries were resequenced paired-end 150 on a NovaSeq 6000 Illumina sequencer for more reads.

Techniques: Comparison