Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Metagenomic analysis of soybean endosphere microbiome to reveal signatures of microbes for health and disease

doi: 10.1186/s43141-023-00535-4

Figure Lengend Snippet: The gene list annotation using the metagenomic database

Article Snippet: Rhodopseudomonas palustris TIE-1 , RPA3585 , 513 , Simkania negevensis Z, ATCC VR-1471 , fabG-B , 744.

Techniques: Sequencing

Journal: Applied and Environmental Microbiology

Article Title: Temperature Affects the Host Range of Rhabdochlamydia porcellionis

doi: 10.1128/aem.00309-23

Figure Lengend Snippet: Permissivity of arthropod and mammalian cell lines to R. porcellionis. (A) The y axis represents the fold change of the number of genome copies per microliter relative to the initial time point. The results are shown as the mean and standard deviation from three biological replicates. (B) R. porcellionis in mammalian and arthropod cell lines at 6 days postinfection. Growth could be observed only in Sf9 cells. The reticulate bodies do not appear to be grouped in an inclusion and seem to be replicating directly in the cytoplasm. The enlarged bodies in the C6/36 cell line are likely aberrant bodies. Bacteria appear to have been internalized in all the other cell lines but failed to replicate. White arrows indicate enlarged bacteria in C6/36 cells and internalized EBs in the other cell lines. Cells were stained with concanavalin A (red), DAPI (blue), and anti- Simkania antibody (green); bar, 10 μm.

Article Snippet: The coverslips were then incubated at room temperature for 2 h in blocking solution with rabbit anti- Simkania negevensis antibodies ( ) (dilution at 1:1,000), rabbit anti- Waddlia chondrophila antibodies ( ) (dilution at 1:1,000), or goat antibodies targeting the major outer membrane protein of Chlamydia trachomatis (dilution at 1:1,000) (LSBio, Seattle, WA, USA).

Techniques: Standard Deviation, Staining

Journal: Applied and Environmental Microbiology

Article Title: Temperature Affects the Host Range of Rhabdochlamydia porcellionis

doi: 10.1128/aem.00309-23

Figure Lengend Snippet: The growth of R. porcellionis in mammalian cells depends on temperature. (A) Growth kinetics of R. porcellionis in Sf9 cells incubated at 20, 28, 33, and 37°C. (B) Growth kinetics of R. porcellionis in mammalian cells incubated at 28°C. In both panel A and panel B, the y axis represents the fold change relative to the initial time point. (C) Doubling time of R. porcellionis in the different cell lines. Doubling times were estimated by dividing 48 h by the log 2 of the highest fold change observed between two consecutive time points. Despite the marked difference between the doubling time in Sf9 and the other cell lines, the Kruskal-Wallis test was not statistically significant ( P = 0.06). (D) IFU count of R. porcellionis grown in different cell lines at 28°C. The cells were fixed at 6 days postinfection. The tendency of infected Sf9 cells to detach from the glass coverslips could induce an underestimation of the IFU count. A one-way ANOVA revealed that there was a statistically significant difference between at least two cell lines ( P value = 0.0002). The plot shows the results of the Tukey honestly significant difference test for the pairwise comparison of the IFU count in the different cell lines (**, <0.01; ***, <0.001). (E) McCoy, A549, and Ishikawa cells infected with R. porcellionis , incubated at 28°C, and fixed at 6 days postinfection. The two enlarged bodies in McCoy and A549 cells are likely aberrant bodies (white arrows). Cells were stained with concanavalin A (red), DAPI (blue), and anti- Simkania antibodies (green). Bar, 10 μm. The results show the mean and standard deviation from three biological replicates.

Article Snippet: The coverslips were then incubated at room temperature for 2 h in blocking solution with rabbit anti- Simkania negevensis antibodies ( ) (dilution at 1:1,000), rabbit anti- Waddlia chondrophila antibodies ( ) (dilution at 1:1,000), or goat antibodies targeting the major outer membrane protein of Chlamydia trachomatis (dilution at 1:1,000) (LSBio, Seattle, WA, USA).

Techniques: Incubation, Infection, Staining, Standard Deviation

Journal: International Journal of Peptides

Article Title: Activity of Cathelicidin Peptides against Simkania negevensis

doi: 10.1155/2011/708710

Figure Lengend Snippet: Activity of cathelicidin peptides at different concentrations on the infectivity of Simkania negevensis.

Article Snippet: The reference strain Z of S. negevensis (American Type Culture Collection VR-1471) was grown in LLC-MK2 cells [ ] in 6-large well plates and elementary bodies (EBs) were purified by use of sucrose gradients [ ], resuspended in sucrose-phosphate-glutamic acid buffer (SPG) pH 7.4 (75 g sucrose, 87 mL 0.2 M Na 2 HPO 4 , 13 mL 0.2 M NaH 2 PO 4 , 0.72 g L-glutamic acid), and frozen in aliquots at −80°C.

Techniques: Activity Assay, Infection