Journal: International Journal of General Medicine

Article Title: Comprehensive Analysis Reveals Distinct Immunological and Prognostic Characteristics of CD276/B7-H3 in Pan-Cancer

doi: 10.2147/IJGM.S395553

Figure Lengend Snippet: Exploration the biological role of CD276 in OV and CESC cells. ( A – D ) Knockdown efficiency of CD276 detected by qPCR in A2780, SKOV3, Hela and Siha cells. ( E – H ) The results of CCK-8 assay showing the decreased levels of CD276 inhibited the proliferation of A2780, SKOV3, Hela and Siha cell lines. Time, hours (**p < 0.01, ***p < 0.001, ****p < 0.0001).

Article Snippet: Two human ovarian cancer cell lines (A2780, SKOV3), a human cervical adenocarcinoma cell line (Hela) and a human cervical squamous cell carcinoma cell line (Siha) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: CCK-8 Assay

Journal: Molecular Cancer

Article Title: Methylation-mediated silencing and tumour suppressive function of hsa-miR-124 in cervical cancer

doi: 10.1186/1476-4598-9-167

Figure Lengend Snippet: Hsa-mi R-124 methylation in primary keratinocytes (EK cells) and cervical cancer cell lines SiHa, CaSki and HeLa. A. hsa-miR-124-1 methylation, B. hsa-miR-124-2 methylation, C. hsa-miR-124-3 methylation. Whereas in primary keratinocytes no methylation was detectable, all cervical cancer cell lines were positive for methylation of hsa-miR-124-1, hsa-miR-124-2 and hsa-miR-124-3. D . In SiHa cells treated with 5000 nM DAC, methylation levels of hsa-miR-124-1 (black), hsa-miR-124-2 (white) and hsa-miR-124-3 (grey) were reduced by more than 50%. Methylation levels of all regions in untreated cells were set to 100%. E . Whereas in untreated and mock (PBS) treated SiHa cells no hsa-miR-124 expression was detectable, SiHa cells treated with 5000 nM DAC showed clear hsa-miR-124 expression.

Article Snippet: The human cervical carcinoma cell lines SiHa, CaSki and HeLa were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Methylation, Expressing

Journal: Molecular Cancer

Article Title: Methylation-mediated silencing and tumour suppressive function of hsa-miR-124 in cervical cancer

doi: 10.1186/1476-4598-9-167

Figure Lengend Snippet: Ectopic expression of hsa-miR-124 in SiHa and CaSki cells. A. Whereas parental cell lines and empty vector control cells (SiHa_ctrl and CaSki_ctrl) showed no detectable expression of hsa-miR-124 , cells transduced with hsa-miR-124 (SiHa_miR-124 and CaSki_miR-124) expressed hsa-miR-124 . Ectopic hsa-miR-124 expression resulted in decreased proliferation rates of B . SiHa_miR-124 (red) and C . CaSki_miR-124 (red) compared to parental (black) and empty vector control cells (grey). In D . results of wound-healing assays in SiHa_miR-124, SiHa_ctrl and SiHa cells are shown, indicating decreased migratory capacity in cells expressing hsa-miR-124 .

Article Snippet: The human cervical carcinoma cell lines SiHa, CaSki and HeLa were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Plasmid Preparation, Transduction

Journal: Molecular Cancer

Article Title: Methylation-mediated silencing and tumour suppressive function of hsa-miR-124 in cervical cancer

doi: 10.1186/1476-4598-9-167

Figure Lengend Snippet: IGFBP7 and SLC25A36 expression in HPV-immortalised cells and cervical cancer cells transduced with hsa-miR-124 . A. Expression levels of IGFBP7 , a potential target gene of hsa-miR-124 , were increased in late passages of FK16B and FK18B cells, also showing increased methylation of hsa-miR-124 , compared to their corresponding early passages. B . Effects of ectopic hsa-miR-124 expression on mRNA expression of IGFBP7 (grey bars) in SiHa and CaSki cells. Expression of SLC25A36 (white bars), a gene without an hsa-miR-124 target site, was also determined as a control. Expression in CaSki_ctrl and SiHa_ctrl was set to 100%, respectively. Results from 3 independent experiments showed that IGFBP7 expression was decreased in CaSki_miR-124 but not in SiHa_miR-124 cells compared to their parental and empty vector control cell lines. Expression of SLC25A36 was similar in cells with and without ectopic hsa-miR-124 expression.

Article Snippet: The human cervical carcinoma cell lines SiHa, CaSki and HeLa were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Transduction, Methylation, Plasmid Preparation

Journal: Cells, Tissues, Organs

Article Title: LncRNA HOTAIR Promotes Chemoresistance by Facilitating Epithelial to Mesenchymal Transition through miR-29b/PTEN/PI3K Signaling in Cervical Cancer

doi: 10.1159/000519844

Figure Lengend Snippet: HOTAIR promoted migration and proliferation of cervical cancer cells in vitro. a Representative images for the transwell assays under different experimental conditions. b Relative cell invasion of Hela and SiHa cells to NC control. (×100). c Representative images for the wound healing assays. d Wound disclosure in the experimental groups. e Representative images for the colony formation assay. f Relative colony formation to control in the experimental groups. g, h The proliferation of Hela and SiHa cells in different experimental groups with time was measured by CCK-8 assay. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01 versus NC.

Article Snippet: Cell Culture Human cervical adenocarcinoma cell lines HeLa (ATCC® CRM-CCL-2TM) and cervical squamous carcinoma cell line SiHa (ATCC® HTB-35 TM) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Migration, In Vitro, Colony Assay, CCK-8 Assay

Journal: Cells, Tissues, Organs

Article Title: LncRNA HOTAIR Promotes Chemoresistance by Facilitating Epithelial to Mesenchymal Transition through miR-29b/PTEN/PI3K Signaling in Cervical Cancer

doi: 10.1159/000519844

Figure Lengend Snippet: HOTAIR facilitated chemoresistance of cervical cancer cells in vitro. CCK-8 assay was conducted to determine the cell viability of Hela and SiHa cells under different treatment of Cisplatin (a, b), Paclitaxel (c, d) and Docetaxel (e, f). The relative chemoresistance was determined by IC50. Data are expressed as mean ± SD. * p < 0.05 versus Control (pcHOTAIR or siRNA).

Article Snippet: Cell Culture Human cervical adenocarcinoma cell lines HeLa (ATCC® CRM-CCL-2TM) and cervical squamous carcinoma cell line SiHa (ATCC® HTB-35 TM) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: In Vitro, CCK-8 Assay

Journal: Cells, Tissues, Organs

Article Title: LncRNA HOTAIR Promotes Chemoresistance by Facilitating Epithelial to Mesenchymal Transition through miR-29b/PTEN/PI3K Signaling in Cervical Cancer

doi: 10.1159/000519844

Figure Lengend Snippet: MiR-29b suppressed proliferation and migration of cervical cancer cells in vitro. a Representative images for the transwell assays under different experimental conditions. b Relative cell invasion of Hela and SiHa cells to NC control. (×100). c Representative images for the wound healing assays. d Wound disclosure in the experimental groups. e, f The proliferation of Hela and SiHa cells in different experimental groups with time was measured by CCK-8 assay. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus NC.

Article Snippet: Cell Culture Human cervical adenocarcinoma cell lines HeLa (ATCC® CRM-CCL-2TM) and cervical squamous carcinoma cell line SiHa (ATCC® HTB-35 TM) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Migration, In Vitro, CCK-8 Assay

Journal: Cells, Tissues, Organs

Article Title: LncRNA HOTAIR Promotes Chemoresistance by Facilitating Epithelial to Mesenchymal Transition through miR-29b/PTEN/PI3K Signaling in Cervical Cancer

doi: 10.1159/000519844

Figure Lengend Snippet: MiR-29b inhibited chemoresistance of cervical cancer cells. CCK-8 assay was conducted to determine the cell viability of Hela and SiHa cells under different treatment of Cisplatin (a, b), Paclitaxel (c, d) and Docetaxel (e, f). The relative chemoresistance was determined by IC50. Data are expressed as mean ± SD.

Article Snippet: Cell Culture Human cervical adenocarcinoma cell lines HeLa (ATCC® CRM-CCL-2TM) and cervical squamous carcinoma cell line SiHa (ATCC® HTB-35 TM) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: CCK-8 Assay

Journal: Cells, Tissues, Organs

Article Title: LncRNA HOTAIR Promotes Chemoresistance by Facilitating Epithelial to Mesenchymal Transition through miR-29b/PTEN/PI3K Signaling in Cervical Cancer

doi: 10.1159/000519844

Figure Lengend Snippet: MiR-29b suppressed EMT transition of cervical cancer cells. a, c Western blot analysis of the expression of epithelial and mesenchymal marker proteins in Hela and SiHa cells. b,d Quantitative analysis of the expression of E-cadherin, N-cadherin and vimentin proteins. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus NC.

Article Snippet: Cell Culture Human cervical adenocarcinoma cell lines HeLa (ATCC® CRM-CCL-2TM) and cervical squamous carcinoma cell line SiHa (ATCC® HTB-35 TM) were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Western Blot, Expressing, Marker

Journal: Cell Death & Disease

Article Title: LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer

doi: 10.1038/s41419-021-03556-4

Figure Lengend Snippet: A High abundance of H3K4me2 aggregation occurred in the CD274 / CD47 promoter region in a variety of tumor cells, including cervical cancer, according to histone ChIP-Seq data from the ENCODE database. B ChIP-Seq performed for SiHa cells also confirmed universal enrichment of H3K4me2 marks in the CD274 / CD47 promoter region. C , D Specific sequences targeting the CD274 / CD47 promoter region. E ChIP-qPCR indicated that the H3K4me2 level in the CD274 promoter region increased significantly after LSD1 knockdown in SiHa and C33A cells ( n = 3 per group); F the H3K4me2 level in the CD47 promoter region underwent the same change ( n = 3 per group). ChIP-qPCR experiments were repeated twice. N.S. not significant. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Human cervical cancer cell lines SiHa and C33A were initially obtained from the American Type Culture Collection (ATCC; Manassas, VA) and were maintained in our laboratory.

Techniques: ChIP-sequencing

Journal: Cell Death & Disease

Article Title: LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer

doi: 10.1038/s41419-021-03556-4

Figure Lengend Snippet: A MicroRNA sequencing indicated that miR-34a, which was predicted to target the 3′UTR of CD47/PD-L1, was upregulated significantly after LSD1 knockdown in SiHa cells. B Changes in miR-34a expression after LSD1 knockdown in SiHa cells were confirmed by qRT-PCR ( n = 3 per group). C , D Changes in the expression of PD-L1/CD47 mRNA in SiHa and C33A cells after transfection with miR-34a mimics or inhibitors ( n = 3 per group). E Western blotting and F flow cytometry showed that miR-34a mimics upregulated the protein expression of CD47/PD-L1 in SiHa and C33A cells, whereas miR-34a inhibitors had the opposite effects. Each experiment was repeated three times. N.S. not significant. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Human cervical cancer cell lines SiHa and C33A were initially obtained from the American Type Culture Collection (ATCC; Manassas, VA) and were maintained in our laboratory.

Techniques: Sequencing, Expressing, Quantitative RT-PCR, Transfection, Western Blot, Flow Cytometry

Journal: Cell Death & Disease

Article Title: LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer

doi: 10.1038/s41419-021-03556-4

Figure Lengend Snippet: A Co-IP assay showed that LSD1 formed a stable complex with wild-type p53 in SiHa cells. B LSD1 knockdown contributes to upregulation of p53 mRNA and protein expression in SiHa cells ( n = 3 per group). C There was a positive correlation between miR-34a and p53 mRNA in cervical cancer according to the normalized counts RNA-sequencing data from TCGA-CESC. D p53 silencing led to downregulation of miR-34a expression in SiHa cells ( n = 3 per group). E Nutlin-3a and doxorubicin were used to stimulate p53 in SiHa cells and contributed to upregulation of miR-34a expression in a dose-dependent manner ( n = 3 per group). Each experiment was repeated three times. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Human cervical cancer cell lines SiHa and C33A were initially obtained from the American Type Culture Collection (ATCC; Manassas, VA) and were maintained in our laboratory.

Techniques: Co-Immunoprecipitation Assay, Expressing, RNA Sequencing Assay