shuffletm t7 competent escherichia coli cells  (New England Biolabs)


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    Name:
    SHuffle T7 Competent E coli
    Description:
    SHuffle T7 Competent E coli 12x0 05 ml
    Catalog Number:
    c3026j
    Price:
    228
    Size:
    12x0 05 ml
    Category:
    Competent Bacteria
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    Structured Review

    New England Biolabs shuffletm t7 competent escherichia coli cells
    SHuffle T7 Competent E coli
    SHuffle T7 Competent E coli 12x0 05 ml
    https://www.bioz.com/result/shuffletm t7 competent escherichia coli cells/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shuffletm t7 competent escherichia coli cells - by Bioz Stars, 2020-08
    97/100 stars

    Related Products / Commonly Used Together

    pro-vcc
    pet32a
    14-kda prodomain

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    Related Articles

    Clone Assay:

    Article Title: The β-Prism Lectin Domain of Vibrio cholerae Hemolysin Promotes Self-assembly of the β-Pore-forming Toxin by a Carbohydrate-independent Mechanism *
    Article Snippet: .. Briefly, pro-VCC, which includes the 14-kDa prodomain (amino acid residues 26–741 of 82-kDa prepro-VCC) , was cloned into the pET32a(+) expression vector (Novagen) and transformed into SHuffleTM T7 competent Escherichia coli cells (New England Biolabs), which provided a nonreducing cytosol appropriate for proper folding of the cytolysin ( ). .. To prepare VCC50 , a stop codon was introduced after the cytolytic domain, and to obtain the mutant VCCD617A , a point mutation was introduced into the pro-VCC nucleotide sequence ( , ).

    Article Title: The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall
    Article Snippet: .. Cloning was done using E. coli DH5α and selection with 30 µg/ml kanamycin; the final expression vector was transformed into SHuffle T7 competent E. coli cells (New England Biolabs). .. LB medium containing selection antibiotic was inoculated with the expression strain and shaken at 200 rpm and 30°C.

    Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation
    Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into SHuffle™ T7 competent Escherichia coli cells (New England Biolabs). .. The hexahistidine-tagged pro-VCC was isolated by chromatography of the cell lysate on Ni2+ -agarose column.

    Selection:

    Article Title: The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall
    Article Snippet: .. Cloning was done using E. coli DH5α and selection with 30 µg/ml kanamycin; the final expression vector was transformed into SHuffle T7 competent E. coli cells (New England Biolabs). .. LB medium containing selection antibiotic was inoculated with the expression strain and shaken at 200 rpm and 30°C.

    Mutagenesis:

    Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation
    Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into SHuffle™ T7 competent Escherichia coli cells (New England Biolabs). .. The hexahistidine-tagged pro-VCC was isolated by chromatography of the cell lysate on Ni2+ -agarose column.

    Isolation:

    Article Title: Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
    Article Snippet: .. EGF-CLIPf isolation and labeling: Expression of recombinant EGF-CLIPf -His6 was performed in SHuffle T7 E. coli (New England Biolabs). .. EGF-CLIPf -His6 was purified from E. coli cell lysate using Ni-NTA Agarose (Qiagen).

    Labeling:

    Article Title: Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
    Article Snippet: .. EGF-CLIPf isolation and labeling: Expression of recombinant EGF-CLIPf -His6 was performed in SHuffle T7 E. coli (New England Biolabs). .. EGF-CLIPf -His6 was purified from E. coli cell lysate using Ni-NTA Agarose (Qiagen).

    Purification:

    Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease
    Article Snippet: .. Protein expression and purification The pET25b (+) vectors that harbor coding genes of αPD-1-ABD-PE, αPD-1, ABD-PE, and αPD-1-PE were transferred into competent Shuffle T7 E. coli cells (New England Biolabs). .. These transformed E. coli cells were cultured in LB broth at 32 °C until the optical density (OD595 ) of the culture reached 0.6.

    other:

    Article Title: Crystal structures of a llama VHH antibody BCD090-M2 targeting human ErbB3 receptor
    Article Snippet: The llama VHH antibody BCD090-M2 was expressed in soluble form in the cytoplasm of E. coli SHuffle T7 Express cells as a SUMO fusion.

    Activity Assay:

    Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation
    Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into SHuffle™ T7 competent Escherichia coli cells (New England Biolabs). .. The hexahistidine-tagged pro-VCC was isolated by chromatography of the cell lysate on Ni2+ -agarose column.

    Expressing:

    Article Title: Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
    Article Snippet: .. EGF-CLIPf isolation and labeling: Expression of recombinant EGF-CLIPf -His6 was performed in SHuffle T7 E. coli (New England Biolabs). .. EGF-CLIPf -His6 was purified from E. coli cell lysate using Ni-NTA Agarose (Qiagen).

    Article Title: The β-Prism Lectin Domain of Vibrio cholerae Hemolysin Promotes Self-assembly of the β-Pore-forming Toxin by a Carbohydrate-independent Mechanism *
    Article Snippet: .. Briefly, pro-VCC, which includes the 14-kDa prodomain (amino acid residues 26–741 of 82-kDa prepro-VCC) , was cloned into the pET32a(+) expression vector (Novagen) and transformed into SHuffleTM T7 competent Escherichia coli cells (New England Biolabs), which provided a nonreducing cytosol appropriate for proper folding of the cytolysin ( ). .. To prepare VCC50 , a stop codon was introduced after the cytolytic domain, and to obtain the mutant VCCD617A , a point mutation was introduced into the pro-VCC nucleotide sequence ( , ).

    Article Title: The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall
    Article Snippet: .. Cloning was done using E. coli DH5α and selection with 30 µg/ml kanamycin; the final expression vector was transformed into SHuffle T7 competent E. coli cells (New England Biolabs). .. LB medium containing selection antibiotic was inoculated with the expression strain and shaken at 200 rpm and 30°C.

    Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease
    Article Snippet: .. Protein expression and purification The pET25b (+) vectors that harbor coding genes of αPD-1-ABD-PE, αPD-1, ABD-PE, and αPD-1-PE were transferred into competent Shuffle T7 E. coli cells (New England Biolabs). .. These transformed E. coli cells were cultured in LB broth at 32 °C until the optical density (OD595 ) of the culture reached 0.6.

    Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation
    Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into SHuffle™ T7 competent Escherichia coli cells (New England Biolabs). .. The hexahistidine-tagged pro-VCC was isolated by chromatography of the cell lysate on Ni2+ -agarose column.

    Transformation Assay:

    Article Title: The β-Prism Lectin Domain of Vibrio cholerae Hemolysin Promotes Self-assembly of the β-Pore-forming Toxin by a Carbohydrate-independent Mechanism *
    Article Snippet: .. Briefly, pro-VCC, which includes the 14-kDa prodomain (amino acid residues 26–741 of 82-kDa prepro-VCC) , was cloned into the pET32a(+) expression vector (Novagen) and transformed into SHuffleTM T7 competent Escherichia coli cells (New England Biolabs), which provided a nonreducing cytosol appropriate for proper folding of the cytolysin ( ). .. To prepare VCC50 , a stop codon was introduced after the cytolytic domain, and to obtain the mutant VCCD617A , a point mutation was introduced into the pro-VCC nucleotide sequence ( , ).

    Article Title: The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall
    Article Snippet: .. Cloning was done using E. coli DH5α and selection with 30 µg/ml kanamycin; the final expression vector was transformed into SHuffle T7 competent E. coli cells (New England Biolabs). .. LB medium containing selection antibiotic was inoculated with the expression strain and shaken at 200 rpm and 30°C.

    Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation
    Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into SHuffle™ T7 competent Escherichia coli cells (New England Biolabs). .. The hexahistidine-tagged pro-VCC was isolated by chromatography of the cell lysate on Ni2+ -agarose column.

    Recombinant:

    Article Title: Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
    Article Snippet: .. EGF-CLIPf isolation and labeling: Expression of recombinant EGF-CLIPf -His6 was performed in SHuffle T7 E. coli (New England Biolabs). .. EGF-CLIPf -His6 was purified from E. coli cell lysate using Ni-NTA Agarose (Qiagen).

    Plasmid Preparation:

    Article Title: The β-Prism Lectin Domain of Vibrio cholerae Hemolysin Promotes Self-assembly of the β-Pore-forming Toxin by a Carbohydrate-independent Mechanism *
    Article Snippet: .. Briefly, pro-VCC, which includes the 14-kDa prodomain (amino acid residues 26–741 of 82-kDa prepro-VCC) , was cloned into the pET32a(+) expression vector (Novagen) and transformed into SHuffleTM T7 competent Escherichia coli cells (New England Biolabs), which provided a nonreducing cytosol appropriate for proper folding of the cytolysin ( ). .. To prepare VCC50 , a stop codon was introduced after the cytolytic domain, and to obtain the mutant VCCD617A , a point mutation was introduced into the pro-VCC nucleotide sequence ( , ).

    Article Title: The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall
    Article Snippet: .. Cloning was done using E. coli DH5α and selection with 30 µg/ml kanamycin; the final expression vector was transformed into SHuffle T7 competent E. coli cells (New England Biolabs). .. LB medium containing selection antibiotic was inoculated with the expression strain and shaken at 200 rpm and 30°C.

    Article Title: Vibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation
    Article Snippet: .. 2.1 Preparation of wild-type and mutant toxins: homogeneity and hemolytic activity The wild-type toxin as well as the mutants were prepared by cloning Pro-VCC , into the peT32a+ expression vector (Novagen) and transformed into SHuffle™ T7 competent Escherichia coli cells (New England Biolabs). .. The hexahistidine-tagged pro-VCC was isolated by chromatography of the cell lysate on Ni2+ -agarose column.

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  • News
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  • Team
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  • Bioz vStars
  • 97
    New England Biolabs shuffletm t7 competent escherichia coli cells
    Shuffletm T7 Competent Escherichia Coli Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffletm t7 competent escherichia coli cells/product/New England Biolabs
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    shuffletm t7 competent escherichia coli cells - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

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