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Horizon Discovery shrna sirna smart pools
Accelerated Cell-Cycle Progression in Cks2 −/− MEFs (A) Time-lapse microscopy of asynchronously growing wild-type, Cks2 −/− and Cks1 −/− MEFs. MEFs were immortalized using <t>shRNA</t> against p53. Wild-type MEFs were infected with a retrovirus expressing histone H2B-GFP, Cks2 −/− and Cks1 −/− MEFs expressed histone H2B-mCherry. Wild-type and knockout MEFs were mixed and imaged. The time between two successive anaphases was recorded for at least 50 cells of each genotype; the mean is shown. An example of a wild-type and Cks2 −/− cell is shown. Alternatively, MEFs were immortalized using the 3T3 protocol, and cell cycle duration was measured as the duration from one cytokinesis to the next for at least 50 cells per genotype. (B) FACS analysis of DNA content to generate cell cycle profiles of asynchronously proliferating wild-type, Cks2 −/− and Cks1 −/− MEFs. (C) Quantification of the cell cycle populations by FACS. (D) Experimental design and example of the FACS pattern of wild-type MEFs at time 0, depicting the G1, BrdU-positive and G2 gates. The ratio of BrdU-positive cells with a G1 DNA content (labeled “early S”) over unlabeled G1 cells was followed over a 24 hr chase.
Shrna Sirna Smart Pools, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/shrna sirna smart pools/product/Horizon Discovery
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
shrna sirna smart pools - by Bioz Stars, 2020-09
85/100 stars

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1) Product Images from "The CDK Subunit CKS2 Counteracts CKS1 to Control Cyclin A/CDK2 Activity in Maintaining Replicative Fidelity and Neurodevelopment"

Article Title: The CDK Subunit CKS2 Counteracts CKS1 to Control Cyclin A/CDK2 Activity in Maintaining Replicative Fidelity and Neurodevelopment

Journal: Developmental Cell

doi: 10.1016/j.devcel.2012.06.018

Accelerated Cell-Cycle Progression in Cks2 −/− MEFs (A) Time-lapse microscopy of asynchronously growing wild-type, Cks2 −/− and Cks1 −/− MEFs. MEFs were immortalized using shRNA against p53. Wild-type MEFs were infected with a retrovirus expressing histone H2B-GFP, Cks2 −/− and Cks1 −/− MEFs expressed histone H2B-mCherry. Wild-type and knockout MEFs were mixed and imaged. The time between two successive anaphases was recorded for at least 50 cells of each genotype; the mean is shown. An example of a wild-type and Cks2 −/− cell is shown. Alternatively, MEFs were immortalized using the 3T3 protocol, and cell cycle duration was measured as the duration from one cytokinesis to the next for at least 50 cells per genotype. (B) FACS analysis of DNA content to generate cell cycle profiles of asynchronously proliferating wild-type, Cks2 −/− and Cks1 −/− MEFs. (C) Quantification of the cell cycle populations by FACS. (D) Experimental design and example of the FACS pattern of wild-type MEFs at time 0, depicting the G1, BrdU-positive and G2 gates. The ratio of BrdU-positive cells with a G1 DNA content (labeled “early S”) over unlabeled G1 cells was followed over a 24 hr chase.
Figure Legend Snippet: Accelerated Cell-Cycle Progression in Cks2 −/− MEFs (A) Time-lapse microscopy of asynchronously growing wild-type, Cks2 −/− and Cks1 −/− MEFs. MEFs were immortalized using shRNA against p53. Wild-type MEFs were infected with a retrovirus expressing histone H2B-GFP, Cks2 −/− and Cks1 −/− MEFs expressed histone H2B-mCherry. Wild-type and knockout MEFs were mixed and imaged. The time between two successive anaphases was recorded for at least 50 cells of each genotype; the mean is shown. An example of a wild-type and Cks2 −/− cell is shown. Alternatively, MEFs were immortalized using the 3T3 protocol, and cell cycle duration was measured as the duration from one cytokinesis to the next for at least 50 cells per genotype. (B) FACS analysis of DNA content to generate cell cycle profiles of asynchronously proliferating wild-type, Cks2 −/− and Cks1 −/− MEFs. (C) Quantification of the cell cycle populations by FACS. (D) Experimental design and example of the FACS pattern of wild-type MEFs at time 0, depicting the G1, BrdU-positive and G2 gates. The ratio of BrdU-positive cells with a G1 DNA content (labeled “early S”) over unlabeled G1 cells was followed over a 24 hr chase.

Techniques Used: Time-lapse Microscopy, shRNA, Infection, Expressing, Knock-Out, FACS, Labeling

Related Articles

Transfection:

Article Title: The CDK Subunit CKS2 Counteracts CKS1 to Control Cyclin A/CDK2 Activity in Maintaining Replicative Fidelity and Neurodevelopment
Article Snippet: .. siRNA and shRNA siRNA Smart pools were purchased from Dharmacon and transfected using Hyperfect (QIAGEN) following the manufacturer's instructions. .. For the shRNA vector against p53, viral particles were generated by standard transfections with helper plasmids in 293T cells.

Article Title: A Feedback Loop Consisting of MicroRNA 23a/27a and the ?-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression
Article Snippet: .. Short interfering RNA (siRNA) smart pools (for KLF3 and SP1) and control siRNA pools were obtained from Dharmacon (D-001210-01-05 for nontargeting siRNA, M-006987-03 for siKLF3, and M-026959-00 for siSP1) and were transfected into K562 cells (100 nM) using DharmaFECT1. .. For erythroid differentiation, the transfected K562 cells were washed with PBS and plated for hemin induction the next day.

Article Title: Transcriptome and Small RNA Deep Sequencing Reveals Deregulation of miRNA Biogenesis in Human Glioma
Article Snippet: .. Small interfering RNA (siRNA) SMART pools (Dharmacon, Lafayette, CO) targeting DICER1 , EIF2C1 , and EIF2C2 were transfected using RNAiMax (Life Technologies, Carlsbad, CA), according to manufacturer’s instructions. .. Cells were harvested after 48 hours, and total RNA was isolated using the mirVana kit (Ambion, Carlsbad, CA).

shRNA:

Article Title: The CDK Subunit CKS2 Counteracts CKS1 to Control Cyclin A/CDK2 Activity in Maintaining Replicative Fidelity and Neurodevelopment
Article Snippet: .. siRNA and shRNA siRNA Smart pools were purchased from Dharmacon and transfected using Hyperfect (QIAGEN) following the manufacturer's instructions. .. For the shRNA vector against p53, viral particles were generated by standard transfections with helper plasmids in 293T cells.

Small Interfering RNA:

Article Title: A Feedback Loop Consisting of MicroRNA 23a/27a and the ?-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression
Article Snippet: .. Short interfering RNA (siRNA) smart pools (for KLF3 and SP1) and control siRNA pools were obtained from Dharmacon (D-001210-01-05 for nontargeting siRNA, M-006987-03 for siKLF3, and M-026959-00 for siSP1) and were transfected into K562 cells (100 nM) using DharmaFECT1. .. For erythroid differentiation, the transfected K562 cells were washed with PBS and plated for hemin induction the next day.

Article Title: Transcriptome and Small RNA Deep Sequencing Reveals Deregulation of miRNA Biogenesis in Human Glioma
Article Snippet: .. Small interfering RNA (siRNA) SMART pools (Dharmacon, Lafayette, CO) targeting DICER1 , EIF2C1 , and EIF2C2 were transfected using RNAiMax (Life Technologies, Carlsbad, CA), according to manufacturer’s instructions. .. Cells were harvested after 48 hours, and total RNA was isolated using the mirVana kit (Ambion, Carlsbad, CA).

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    Horizon Discovery nm drp1 sirna smart pool accell probes
    Nm Drp1 Sirna Smart Pool Accell Probes, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nm drp1 sirna smart pool accell probes/product/Horizon Discovery
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nm drp1 sirna smart pool accell probes - by Bioz Stars, 2020-09
    92/100 stars
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