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Horizon Discovery shrna mkp1 sirna
Regulation of the MAPK response to pore-formation is <t>MKP1</t> independent. (A) A549 cells were transfected with 2.5 µg of MKP1 or scrambled <t>siRNA</t> per 1×10 6 cells and stimulated 24 hrs post-transfection with 200 ng/ml of purified Ply for the indicated times. Transfection with MKP1 siRNA inhibited MKP1 expression but did not have an effect on Ply-induced MAPK phosphorylation.
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1) Product Images from "Phosphatase-Dependent Regulation of Epithelial Mitogen-Activated Protein Kinase Responses to Toxin-Induced Membrane Pores"

Article Title: Phosphatase-Dependent Regulation of Epithelial Mitogen-Activated Protein Kinase Responses to Toxin-Induced Membrane Pores

Journal: PLoS ONE

doi: 10.1371/journal.pone.0008076

Regulation of the MAPK response to pore-formation is MKP1 independent. (A) A549 cells were transfected with 2.5 µg of MKP1 or scrambled siRNA per 1×10 6 cells and stimulated 24 hrs post-transfection with 200 ng/ml of purified Ply for the indicated times. Transfection with MKP1 siRNA inhibited MKP1 expression but did not have an effect on Ply-induced MAPK phosphorylation.
Figure Legend Snippet: Regulation of the MAPK response to pore-formation is MKP1 independent. (A) A549 cells were transfected with 2.5 µg of MKP1 or scrambled siRNA per 1×10 6 cells and stimulated 24 hrs post-transfection with 200 ng/ml of purified Ply for the indicated times. Transfection with MKP1 siRNA inhibited MKP1 expression but did not have an effect on Ply-induced MAPK phosphorylation.

Techniques Used: Transfection, Purification, Expressing

PP1 and PP2A mediate inactivation of the epithelial MAPK response to pore-formation. (A)A549 cells were transfected with 2 µg PP1 or scrambled siRNA per 1×10 6 cells and stimulated 54 hrs post-transfection with 100 ng/ml of purified Ply. Transfection with PP1 siRNA leads to reduced PP1 expression and a corresponding increase in Ply-induced p38 and JNK phosphorylation. (B) A549 cells were transfected with 6 µg PP2Aα/β or control shRNA per 1×10 6 cells and stimulated 60 hrs post-transfection with 100 ng/ml of purified Ply. Transfection with PP2Aα/β shRNA leads to reduced PP2A expression and a moderate increase in p38 phosphorylation.
Figure Legend Snippet: PP1 and PP2A mediate inactivation of the epithelial MAPK response to pore-formation. (A)A549 cells were transfected with 2 µg PP1 or scrambled siRNA per 1×10 6 cells and stimulated 54 hrs post-transfection with 100 ng/ml of purified Ply. Transfection with PP1 siRNA leads to reduced PP1 expression and a corresponding increase in Ply-induced p38 and JNK phosphorylation. (B) A549 cells were transfected with 6 µg PP2Aα/β or control shRNA per 1×10 6 cells and stimulated 60 hrs post-transfection with 100 ng/ml of purified Ply. Transfection with PP2Aα/β shRNA leads to reduced PP2A expression and a moderate increase in p38 phosphorylation.

Techniques Used: Transfection, Purification, Expressing, shRNA

Related Articles

shRNA:

Article Title: Phosphatase-Dependent Regulation of Epithelial Mitogen-Activated Protein Kinase Responses to Toxin-Induced Membrane Pores
Article Snippet: .. siRNA and shRNA MKP1 siRNA was from Dharmacon (Lafayette, CO). .. PP1 and scrambled siRNAs were from Santa Cruz Biotechnology (Santa Cruz, CA), and PP2Aα/β and GFP control shRNA were constructed by William Hahn and obtained from Addgene (Cambridge, MA) (Addgene plasmids 10676, 15249, and 15250) .