shrimp hepatopancreas alp  (New England Biolabs)


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    Name:
    Shrimp Alkaline Phosphatase rSAP
    Description:
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    Catalog Number:
    M0371L
    Price:
    248
    Category:
    Alkaline Phosphatases
    Size:
    2 500 units
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    Structured Review

    New England Biolabs shrimp hepatopancreas alp
    Shrimp Alkaline Phosphatase rSAP
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    https://www.bioz.com/result/shrimp hepatopancreas alp/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shrimp hepatopancreas alp - by Bioz Stars, 2021-06
    99/100 stars

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    1) Product Images from "Innate Biomineralization"

    Article Title: Innate Biomineralization

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21144820

    Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.
    Figure Legend Snippet: Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.

    Techniques Used: Staining, Cell Culture, Cell Differentiation, Titration

    Related Articles

    Cell Culture:

    Article Title: Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases
    Article Snippet: .. Methods The following materials were obtained from commercial sources: native PAI-1, secreted into the cell culture medium from human fibrosarcoma cell line HT 1080 (Biopool); single-chain tPA and two-chain tPA standard (Biopool); uPA, plasmin and thrombin were from Calbiochem; the chromogenic substrate for uPA, pGlu-Gly-Arg p-Nitroanilide (S-2444), the chromogenic substrate for tPA and H-D-Ile-Pro-Arg-pNA-2HCl (S-2288) from Chromgenix AB, chromogenic substrate for thrombin, H-D-Phe-pipecolyl-Arg-pNA(S-2238) and chromogenic substrate for plasmin, N-p-Tosyl-Gly-Pro-Lys-p-Nitroanilide from Sigma); heparin-Sepharose, monoclonal antibody against PAI-1 from American Diagnostica, Inc.; recombinant N-glycanase (Genzyme diagnostics); the pRSET(A) vector, baculovirus transfer vector pAcSG2 and BaculoGold transfection kit from Pharmgen; Sf9 cells (Spodoptera frugiperda), High Five cell (Trichoplusia ni) from Invitrogen; Restriction enzymes, T4 ligase and shrimp phosphatase were purchased from New England Biolab. .. BCA protein assay reagent from Pierce; A full-length cDNA of human PAI-1 (pPAI-1-AI) [ ] was generously provided by Prof. Keld Danö, The Finsen Laboratory.

    Recombinant:

    Article Title: Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases
    Article Snippet: .. Methods The following materials were obtained from commercial sources: native PAI-1, secreted into the cell culture medium from human fibrosarcoma cell line HT 1080 (Biopool); single-chain tPA and two-chain tPA standard (Biopool); uPA, plasmin and thrombin were from Calbiochem; the chromogenic substrate for uPA, pGlu-Gly-Arg p-Nitroanilide (S-2444), the chromogenic substrate for tPA and H-D-Ile-Pro-Arg-pNA-2HCl (S-2288) from Chromgenix AB, chromogenic substrate for thrombin, H-D-Phe-pipecolyl-Arg-pNA(S-2238) and chromogenic substrate for plasmin, N-p-Tosyl-Gly-Pro-Lys-p-Nitroanilide from Sigma); heparin-Sepharose, monoclonal antibody against PAI-1 from American Diagnostica, Inc.; recombinant N-glycanase (Genzyme diagnostics); the pRSET(A) vector, baculovirus transfer vector pAcSG2 and BaculoGold transfection kit from Pharmgen; Sf9 cells (Spodoptera frugiperda), High Five cell (Trichoplusia ni) from Invitrogen; Restriction enzymes, T4 ligase and shrimp phosphatase were purchased from New England Biolab. .. BCA protein assay reagent from Pierce; A full-length cDNA of human PAI-1 (pPAI-1-AI) [ ] was generously provided by Prof. Keld Danö, The Finsen Laboratory.

    Plasmid Preparation:

    Article Title: Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases
    Article Snippet: .. Methods The following materials were obtained from commercial sources: native PAI-1, secreted into the cell culture medium from human fibrosarcoma cell line HT 1080 (Biopool); single-chain tPA and two-chain tPA standard (Biopool); uPA, plasmin and thrombin were from Calbiochem; the chromogenic substrate for uPA, pGlu-Gly-Arg p-Nitroanilide (S-2444), the chromogenic substrate for tPA and H-D-Ile-Pro-Arg-pNA-2HCl (S-2288) from Chromgenix AB, chromogenic substrate for thrombin, H-D-Phe-pipecolyl-Arg-pNA(S-2238) and chromogenic substrate for plasmin, N-p-Tosyl-Gly-Pro-Lys-p-Nitroanilide from Sigma); heparin-Sepharose, monoclonal antibody against PAI-1 from American Diagnostica, Inc.; recombinant N-glycanase (Genzyme diagnostics); the pRSET(A) vector, baculovirus transfer vector pAcSG2 and BaculoGold transfection kit from Pharmgen; Sf9 cells (Spodoptera frugiperda), High Five cell (Trichoplusia ni) from Invitrogen; Restriction enzymes, T4 ligase and shrimp phosphatase were purchased from New England Biolab. .. BCA protein assay reagent from Pierce; A full-length cDNA of human PAI-1 (pPAI-1-AI) [ ] was generously provided by Prof. Keld Danö, The Finsen Laboratory.

    Transfection:

    Article Title: Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases
    Article Snippet: .. Methods The following materials were obtained from commercial sources: native PAI-1, secreted into the cell culture medium from human fibrosarcoma cell line HT 1080 (Biopool); single-chain tPA and two-chain tPA standard (Biopool); uPA, plasmin and thrombin were from Calbiochem; the chromogenic substrate for uPA, pGlu-Gly-Arg p-Nitroanilide (S-2444), the chromogenic substrate for tPA and H-D-Ile-Pro-Arg-pNA-2HCl (S-2288) from Chromgenix AB, chromogenic substrate for thrombin, H-D-Phe-pipecolyl-Arg-pNA(S-2238) and chromogenic substrate for plasmin, N-p-Tosyl-Gly-Pro-Lys-p-Nitroanilide from Sigma); heparin-Sepharose, monoclonal antibody against PAI-1 from American Diagnostica, Inc.; recombinant N-glycanase (Genzyme diagnostics); the pRSET(A) vector, baculovirus transfer vector pAcSG2 and BaculoGold transfection kit from Pharmgen; Sf9 cells (Spodoptera frugiperda), High Five cell (Trichoplusia ni) from Invitrogen; Restriction enzymes, T4 ligase and shrimp phosphatase were purchased from New England Biolab. .. BCA protein assay reagent from Pierce; A full-length cDNA of human PAI-1 (pPAI-1-AI) [ ] was generously provided by Prof. Keld Danö, The Finsen Laboratory.

    Polymerase Chain Reaction:

    Article Title: Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeria monocytogenes
    Article Snippet: PCR amplification of the five selected genes was carried out using primers and conditions described in Additional file . .. PCR fragments were purified using Exonuclease I (0.5 U/μl) and Shrimp alkaline phosphatase (0.05 U/μl) (USB, NEB) and sequenced (at the Biotechnology Resource Center, Cornell University) using Big Dye Terminator chemistry and AmpliTaq-FS DNA Polymerase and an automated 3730 DNA Analyzer. ..

    Article Title: Integron Diversity in Heavy-Metal-Contaminated Mine Tailings and Inferences about Integron Evolution
    Article Snippet: .. Inserted sequences were PCR amplified using the primers M13F and M13R and cleaned using exonuclease I and shrimp alkaline phosphatase (New England Biolabs, Beverly, Mass.) or QIAquick PCR purification columns (Qiagen, Valencia, Calif.). ..

    Purification:

    Article Title: Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeria monocytogenes
    Article Snippet: PCR amplification of the five selected genes was carried out using primers and conditions described in Additional file . .. PCR fragments were purified using Exonuclease I (0.5 U/μl) and Shrimp alkaline phosphatase (0.05 U/μl) (USB, NEB) and sequenced (at the Biotechnology Resource Center, Cornell University) using Big Dye Terminator chemistry and AmpliTaq-FS DNA Polymerase and an automated 3730 DNA Analyzer. ..

    Article Title: Integron Diversity in Heavy-Metal-Contaminated Mine Tailings and Inferences about Integron Evolution
    Article Snippet: .. Inserted sequences were PCR amplified using the primers M13F and M13R and cleaned using exonuclease I and shrimp alkaline phosphatase (New England Biolabs, Beverly, Mass.) or QIAquick PCR purification columns (Qiagen, Valencia, Calif.). ..

    Amplification:

    Article Title: Integron Diversity in Heavy-Metal-Contaminated Mine Tailings and Inferences about Integron Evolution
    Article Snippet: .. Inserted sequences were PCR amplified using the primers M13F and M13R and cleaned using exonuclease I and shrimp alkaline phosphatase (New England Biolabs, Beverly, Mass.) or QIAquick PCR purification columns (Qiagen, Valencia, Calif.). ..

    other:

    Article Title: Extensive transcriptional heterogeneity revealed by isoform profiling
    Article Snippet: RNA was dephosphorylated using Shrimp Alkaline Phosphatase as described earlier, but instead of proceeding to treatment with Tobacco Acid Pyrophosphatase, RNA was rephosphorylated for 1 h at 37°C using T4 Polynucleotide Kinase (NEB).

    Incubation:

    Article Title: A novel NGS library preparation method to characterize native termini of fragmented DNA
    Article Snippet: Preparing template DNA termini for adapter ligationThe termini of template DNA molecules, including control oligos, were prepared for adapter ligation. .. Up to 1 pmol, DNA ends were dephosphorylated in a 20 μl reaction using rapid Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs) and 1× CutSmart buffer incubated at 37°C for 30 min followed by a 10-min heat inactivation at 65°C. .. DNA was then 5′ phosphorylated by bringing the heat-inactivated 20 μl rSAP reaction up to 40 μl using 20 units of T4 Polynucleotide Kinase (New England Biolabs), and a 5% final concentration of PEG 8000.

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  • 99
    New England Biolabs shrimp hepatopancreas alp
    Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase <t>(ALP)</t> are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP <t>(CIP),</t> or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.
    Shrimp Hepatopancreas Alp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp hepatopancreas alp/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.

    Journal: International Journal of Molecular Sciences

    Article Title: Innate Biomineralization

    doi: 10.3390/ijms21144820

    Figure Lengend Snippet: Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.

    Article Snippet: CIP (≥10,000 U/mL), shrimp hepatopancreas ALP (≥1000 unit/mL), and p-nitrophenyl phosphate kits were purchased from New England BioLabs (Ipswich, MA, USA).

    Techniques: Staining, Cell Culture, Cell Differentiation, Titration