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GE Healthcare shrimp alkaline phosphatase
Shrimp Alkaline Phosphatase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 224 article reviews
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shrimp alkaline phosphatase - by Bioz Stars, 2020-01
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Clone Assay:

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA). .. A pUC57-mHCV (m stands for mutated), with an inserted sequence of 144 nt derived from HCV subtype 1a polyprotein, reference sequence AF009606.1, position 130 to 273 cloned into the Bam HI site, was purchased from GenScript (Piscataway, NJ, USA).

Article Title: Human and Murine Clonal CD8+ T Cell Expansions Arise during Tuberculosis Because of TCR Selection
Article Snippet: For TCR product sequencing, a total of 12 μl of the products from the second round of the nested PCR amplification was combined with 1.5 μl of 10X shrimp alkaline phosphatase reaction buffer (200 mM Tris-HCl (pH 8.0) and 100 mM MgCl2), 1 U of shrimp alkaline phosphatase (Amersham Biosciences), and 1 U of exonuclease I (New England Biolabs), and water to total 15 μl. .. The sequences of the four TCRs cloned are shown in .

Amplification:

Article Title: Demographic History of a Recent Invasion of House Mice on the Isolated Island of Gough
Article Snippet: The mitochondrial DNA (mtDNA) d-loop was amplified in all samples (934bps; ). .. PCR products were purified using Exonuclease I and Shrimp Alkaline Phosphatase (Amersham Biosciences).

Article Title: Major pathologic response and RAD51 predict survival in lung cancer patients receiving neoadjuvant chemotherapy
Article Snippet: .. For direct sequencing, all PCR amplification products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ) and sequenced by the MD Anderson Core Sequencing and Microarray Facility. .. Immunohistochemical staining for biomarkers was performed as described previously .

Article Title: XPD Polymorphisms and Risk of Squamous Cell Carcinoma of the Head and Neck in a Korean Sample
Article Snippet: One unit of shrimp alkaline phosphatase (Amersham Life Science, Cleveland, OH, USA) was added to the reaction mixture to clean up the primer extension reaction products, and the mixture was incubated at 37℃ for one hour, followed by 15 min at 72℃ for enzyme inactivation. .. The amplified material and Genescan 120 Liz size-standard solution (Applied Biosystems) were added to Hi-Di formamide (Applied Biosystems) and this was all reacted at 95℃ for five minutes, and then it was incubated on ice for five minutes.

Article Title: Root-associated fungal communities in three Pyroleae species and their mycobiont sharing with surrounding trees in subalpine coniferous forests on Mount Fuji, Japan
Article Snippet: .. Amplification products were purified using a PCR clean-up kit containing exonuclease I and shrimp alkaline phosphatase (GE Healthcare, Hertfordshire, UK). .. Sanger sequencing was performed using the Big Dye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and ITS1 or ITS4.

Article Title: Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya
Article Snippet: Paragraph title: DNA isolation and PCR amplification ... PCR products were purified for direct sequencing by enzymatic treatment using exonuclease I and shrimp alkaline phosphatase (PCR Product Presequencing Kit, Amersham).

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: The amplicon was purified from an agarose gel and inserted into the pGEM-T Easy vector (Promega), generating the pGEM-T Easy-MS2 plasmid. pGEM-T Easy-MS2 was subsequently digested with Not I, generating a fragment of 3,604 bp containing the MS2 genome, which was purified and filled in using the Klenow fragment of DNA polymerase (New England Biolabs, Herts, United Kingdom). pET-47b(+) (Novagen, Darmstadt, Germany) was digested with Xba I and Xho I enzymes (New England Biolabs) liberating a 255 bp fragment of the polylinker region. .. After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

Article Title: Bias in effect size of systemic lupus erythematosus susceptibility loci across Europe: a case-control study
Article Snippet: Genotyping DNA samples were amplified in a multiplex PCR with the KAPA2G fast HotStart (Kapa Biosystems, Woburn, MA, USA) in a final volume of 10 μl (20 ng genomic DNA), using 3 mM MgCl2 and 0.2 μM each primer. .. Products were purified by Exo-SAP digestion with exonuclease I (Epicentre, Madison, WI, USA) and shrimp alkaline phosphatase (GE Healthcare, Barcelona, Spain).

Article Title: Human and Murine Clonal CD8+ T Cell Expansions Arise during Tuberculosis Because of TCR Selection
Article Snippet: .. For TCR product sequencing, a total of 12 μl of the products from the second round of the nested PCR amplification was combined with 1.5 μl of 10X shrimp alkaline phosphatase reaction buffer (200 mM Tris-HCl (pH 8.0) and 100 mM MgCl2), 1 U of shrimp alkaline phosphatase (Amersham Biosciences), and 1 U of exonuclease I (New England Biolabs), and water to total 15 μl. ..

Synthesized:

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: MATERIALS AND METHODS Internal control construction - Complementary DNA from the MS2 RNA (Roche, Mannheim, Germany) was synthesized using the primer CLONMS2-R ( ) and ImProm II reverse transcriptase (Promega, Madison, WI, USA). .. After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

Construct:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The plasmid constructed for conjugation in A. actinomycetemcomitans is based on the mobilizable plasmid pGP704 ( ). .. The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK).

Electrophoresis:

Article Title: XPD Polymorphisms and Risk of Squamous Cell Carcinoma of the Head and Neck in a Korean Sample
Article Snippet: One unit of shrimp alkaline phosphatase (Amersham Life Science, Cleveland, OH, USA) was added to the reaction mixture to clean up the primer extension reaction products, and the mixture was incubated at 37℃ for one hour, followed by 15 min at 72℃ for enzyme inactivation. .. Electrophoresis was performed on an ABI Prism 3100 Genetic Analyzer (Applied Biosystems) and the genes were analyzed using ABI Prism GeneScan and Genotyper software.

Microarray:

Article Title: Major pathologic response and RAD51 predict survival in lung cancer patients receiving neoadjuvant chemotherapy
Article Snippet: .. For direct sequencing, all PCR amplification products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ) and sequenced by the MD Anderson Core Sequencing and Microarray Facility. .. Immunohistochemical staining for biomarkers was performed as described previously .

Incubation:

Article Title: Antitumor activity of pan‐ HER inhibitors in HER2‐positive gastric cancer, et al. Antitumor activity of pan‐HER inhibitors in HER2‐positive gastric cancer
Article Snippet: .. The sequences of the primers were the same as previously reported., The detailed PCR protocol has been previously reported, and all the PCR products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences). .. DNA sequence alterations were evaluated using MutationTaster2.

Article Title: Major pathologic response and RAD51 predict survival in lung cancer patients receiving neoadjuvant chemotherapy
Article Snippet: .. For direct sequencing, all PCR amplification products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ) and sequenced by the MD Anderson Core Sequencing and Microarray Facility. .. Immunohistochemical staining for biomarkers was performed as described previously .

Article Title: XPD Polymorphisms and Risk of Squamous Cell Carcinoma of the Head and Neck in a Korean Sample
Article Snippet: .. One unit of shrimp alkaline phosphatase (Amersham Life Science, Cleveland, OH, USA) was added to the reaction mixture to clean up the primer extension reaction products, and the mixture was incubated at 37℃ for one hour, followed by 15 min at 72℃ for enzyme inactivation. .. The amplified material and Genescan 120 Liz size-standard solution (Applied Biosystems) were added to Hi-Di formamide (Applied Biosystems) and this was all reacted at 95℃ for five minutes, and then it was incubated on ice for five minutes.

Article Title: Exposure to Polycyclic Aromatic Hydrocarbons Among Never Smokers in Golestan Province, Iran, an Area of High Incidence of Esophageal Cancer - a Cross-Sectional Study with Repeated Measurement of Urinary 1-OHPG in Two Seasons
Article Snippet: .. Following SBE, the samples were incubated at 37°C (1 h) with 1 unit shrimp alkaline phosphatase (Amersham) to degrade the unincorporated dideoxynucleotide triphosphates. .. Two multiplex genotyping experiments (as described above) were used for SBE reactions.

Modification:

Article Title: Root-associated fungal communities in three Pyroleae species and their mycobiont sharing with surrounding trees in subalpine coniferous forests on Mount Fuji, Japan
Article Snippet: Briefly, each Pyroleae or ECM root tip was placed in a 2.0-ml tube, pulverized using a bead-beater, and DNA was extracted using a modified cetyl trimethyl ammonium bromide method. .. Amplification products were purified using a PCR clean-up kit containing exonuclease I and shrimp alkaline phosphatase (GE Healthcare, Hertfordshire, UK).

Transformation Assay:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK). .. The ligation mixture was transformed into electrocompetent DH5α(λpir) E. coli cells.

Derivative Assay:

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA). .. A pUC57-mHCV (m stands for mutated), with an inserted sequence of 144 nt derived from HCV subtype 1a polyprotein, reference sequence AF009606.1, position 130 to 273 cloned into the Bam HI site, was purchased from GenScript (Piscataway, NJ, USA).

Conjugation Assay:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The plasmid constructed for conjugation in A. actinomycetemcomitans is based on the mobilizable plasmid pGP704 ( ). .. The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK).

Inverse PCR:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK). .. The resulting plasmid, pVT1460, was used as the template for inverse PCR to delete the β-lactamase gene as described previously ( ).

Ligation:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK). .. The ligation mixture was transformed into electrocompetent DH5α(λpir) E. coli cells.

Generated:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: An isogenic mutant of VT1169 with the morC gene deleted was generated by conjugation using a non-replicating broad host range plasmid ( ). .. The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK).

DNA Sequencing:

Article Title: Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya
Article Snippet: PCR products were purified for direct sequencing by enzymatic treatment using exonuclease I and shrimp alkaline phosphatase (PCR Product Presequencing Kit, Amersham). .. All DNA sequencing was carried out by direct cycle sequencing on both strands of purified PCR DNA products from PCR amplification.

Sequencing:

Article Title: Demographic History of a Recent Invasion of House Mice on the Isolated Island of Gough
Article Snippet: Paragraph title: Genotyping and sequencing ... PCR products were purified using Exonuclease I and Shrimp Alkaline Phosphatase (Amersham Biosciences).

Article Title: Antitumor activity of pan‐ HER inhibitors in HER2‐positive gastric cancer, et al. Antitumor activity of pan‐HER inhibitors in HER2‐positive gastric cancer
Article Snippet: Paragraph title: 2.7. Direct sequencing assay ... The sequences of the primers were the same as previously reported., The detailed PCR protocol has been previously reported, and all the PCR products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences).

Article Title: Major pathologic response and RAD51 predict survival in lung cancer patients receiving neoadjuvant chemotherapy
Article Snippet: .. For direct sequencing, all PCR amplification products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ) and sequenced by the MD Anderson Core Sequencing and Microarray Facility. .. Immunohistochemical staining for biomarkers was performed as described previously .

Article Title: Root-associated fungal communities in three Pyroleae species and their mycobiont sharing with surrounding trees in subalpine coniferous forests on Mount Fuji, Japan
Article Snippet: Amplification products were purified using a PCR clean-up kit containing exonuclease I and shrimp alkaline phosphatase (GE Healthcare, Hertfordshire, UK). .. Sanger sequencing was performed using the Big Dye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and ITS1 or ITS4.

Article Title: Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya
Article Snippet: .. PCR products were purified for direct sequencing by enzymatic treatment using exonuclease I and shrimp alkaline phosphatase (PCR Product Presequencing Kit, Amersham). .. All DNA sequencing was carried out by direct cycle sequencing on both strands of purified PCR DNA products from PCR amplification.

Article Title: Novel TCAP Mutation c.32C > A Causing Limb Girdle Muscular Dystrophy 2G
Article Snippet: PCR products were purified by treating with exonuclease I and Shrimp alkaline phosphatase (Amersham, Piscataway, NJ) at 37°C and 80°C for 15 minutes each. .. Sequencing of PCR products was carried out using 100.0 ng (2.0 µl) of PCR product and 4 pmol (1.0 µl) of primer (forward and reverse in separate reactions), and 4.0 µl of BigDye Terminator ready mixture (Applied Biosystem, Foster city, USA).

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA). .. A pUC57-mHCV (m stands for mutated), with an inserted sequence of 144 nt derived from HCV subtype 1a polyprotein, reference sequence AF009606.1, position 130 to 273 cloned into the Bam HI site, was purchased from GenScript (Piscataway, NJ, USA).

Article Title: Human and Murine Clonal CD8+ T Cell Expansions Arise during Tuberculosis Because of TCR Selection
Article Snippet: .. For TCR product sequencing, a total of 12 μl of the products from the second round of the nested PCR amplification was combined with 1.5 μl of 10X shrimp alkaline phosphatase reaction buffer (200 mM Tris-HCl (pH 8.0) and 100 mM MgCl2), 1 U of shrimp alkaline phosphatase (Amersham Biosciences), and 1 U of exonuclease I (New England Biolabs), and water to total 15 μl. ..

DNA Extraction:

Article Title: Major pathologic response and RAD51 predict survival in lung cancer patients receiving neoadjuvant chemotherapy
Article Snippet: Paragraph title: DNA extraction and mutation analysis ... For direct sequencing, all PCR amplification products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ) and sequenced by the MD Anderson Core Sequencing and Microarray Facility.

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK). .. A. actinomycetemcomitans genomic DNA from VT1169 was purified using the Puregene genomic DNA isolation kit (Qiagen, Valencia, CA) and used as a template for PCR.

Article Title: Root-associated fungal communities in three Pyroleae species and their mycobiont sharing with surrounding trees in subalpine coniferous forests on Mount Fuji, Japan
Article Snippet: DNA extraction and molecular identification followed that described elsewhere (Miyamoto et al. ). .. Amplification products were purified using a PCR clean-up kit containing exonuclease I and shrimp alkaline phosphatase (GE Healthcare, Hertfordshire, UK).

Article Title: Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya
Article Snippet: Paragraph title: DNA isolation and PCR amplification ... PCR products were purified for direct sequencing by enzymatic treatment using exonuclease I and shrimp alkaline phosphatase (PCR Product Presequencing Kit, Amersham).

Mutagenesis:

Article Title: Major pathologic response and RAD51 predict survival in lung cancer patients receiving neoadjuvant chemotherapy
Article Snippet: Paragraph title: DNA extraction and mutation analysis ... For direct sequencing, all PCR amplification products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ) and sequenced by the MD Anderson Core Sequencing and Microarray Facility.

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: An isogenic mutant of VT1169 with the morC gene deleted was generated by conjugation using a non-replicating broad host range plasmid ( ). .. The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK).

Purification:

Article Title: Demographic History of a Recent Invasion of House Mice on the Isolated Island of Gough
Article Snippet: .. PCR products were purified using Exonuclease I and Shrimp Alkaline Phosphatase (Amersham Biosciences). .. PCR products were sequenced using the Big Dye Terminator v3.1 (Applied Biosystems) cycle sequencing kit and run on an Applied Biosystems 3730xl capillary sequencer (Life Technology, Carlsbad, CA).

Article Title: Major pathologic response and RAD51 predict survival in lung cancer patients receiving neoadjuvant chemotherapy
Article Snippet: Subsequently, the biotinylated PCR products were purified and made into single‐stranded DNA to which a sequencing primer was annealed using a vacuum prep tool (Pyrosequencing, Inc., Westborough, MA). .. For direct sequencing, all PCR amplification products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences, Piscataway, NJ) and sequenced by the MD Anderson Core Sequencing and Microarray Facility.

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK). .. A. actinomycetemcomitans genomic DNA from VT1169 was purified using the Puregene genomic DNA isolation kit (Qiagen, Valencia, CA) and used as a template for PCR.

Article Title: Root-associated fungal communities in three Pyroleae species and their mycobiont sharing with surrounding trees in subalpine coniferous forests on Mount Fuji, Japan
Article Snippet: .. Amplification products were purified using a PCR clean-up kit containing exonuclease I and shrimp alkaline phosphatase (GE Healthcare, Hertfordshire, UK). .. Sanger sequencing was performed using the Big Dye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and ITS1 or ITS4.

Article Title: Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya
Article Snippet: .. PCR products were purified for direct sequencing by enzymatic treatment using exonuclease I and shrimp alkaline phosphatase (PCR Product Presequencing Kit, Amersham). .. All DNA sequencing was carried out by direct cycle sequencing on both strands of purified PCR DNA products from PCR amplification.

Article Title: Novel TCAP Mutation c.32C > A Causing Limb Girdle Muscular Dystrophy 2G
Article Snippet: .. PCR products were purified by treating with exonuclease I and Shrimp alkaline phosphatase (Amersham, Piscataway, NJ) at 37°C and 80°C for 15 minutes each. .. Sequencing of PCR products was carried out using 100.0 ng (2.0 µl) of PCR product and 4 pmol (1.0 µl) of primer (forward and reverse in separate reactions), and 4.0 µl of BigDye Terminator ready mixture (Applied Biosystem, Foster city, USA).

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: .. After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA). .. The 3,604 bp region originated from the pGEM-T Easy-MS2 excision was ligated to pET-47b(+), generating pET-47b(+)-MS2.

Article Title: Bias in effect size of systemic lupus erythematosus susceptibility loci across Europe: a case-control study
Article Snippet: .. Products were purified by Exo-SAP digestion with exonuclease I (Epicentre, Madison, WI, USA) and shrimp alkaline phosphatase (GE Healthcare, Barcelona, Spain). .. Subsequently, single-base extension reactions were performed with the SNaPshot Multiplex kit (Applied Biosystems, Foster City, CA, USA).

Polymerase Chain Reaction:

Article Title: Demographic History of a Recent Invasion of House Mice on the Isolated Island of Gough
Article Snippet: .. PCR products were purified using Exonuclease I and Shrimp Alkaline Phosphatase (Amersham Biosciences). .. PCR products were sequenced using the Big Dye Terminator v3.1 (Applied Biosystems) cycle sequencing kit and run on an Applied Biosystems 3730xl capillary sequencer (Life Technology, Carlsbad, CA).

Article Title: Antitumor activity of pan‐ HER inhibitors in HER2‐positive gastric cancer, et al. Antitumor activity of pan‐HER inhibitors in HER2‐positive gastric cancer
Article Snippet: .. The sequences of the primers were the same as previously reported., The detailed PCR protocol has been previously reported, and all the PCR products were incubated using exonuclease I and shrimp alkaline phosphatase (Amersham Biosciences). .. DNA sequence alterations were evaluated using MutationTaster2.

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK). .. A. actinomycetemcomitans genomic DNA from VT1169 was purified using the Puregene genomic DNA isolation kit (Qiagen, Valencia, CA) and used as a template for PCR.

Article Title: XPD Polymorphisms and Risk of Squamous Cell Carcinoma of the Head and Neck in a Korean Sample
Article Snippet: Polymerase chain reaction (PCR) using a GeneAmp PCR System 9700 thermal cycler (Applied Biosystems, Foster City, CA, USA) was performed on the samples, and the samples contained 1.25 pM of each primer, 5 ng of genomic DNA, 250 µM of the dNTPs and 0.15U Taq DNA polymerase. .. One unit of shrimp alkaline phosphatase (Amersham Life Science, Cleveland, OH, USA) was added to the reaction mixture to clean up the primer extension reaction products, and the mixture was incubated at 37℃ for one hour, followed by 15 min at 72℃ for enzyme inactivation.

Article Title: Root-associated fungal communities in three Pyroleae species and their mycobiont sharing with surrounding trees in subalpine coniferous forests on Mount Fuji, Japan
Article Snippet: .. Amplification products were purified using a PCR clean-up kit containing exonuclease I and shrimp alkaline phosphatase (GE Healthcare, Hertfordshire, UK). .. Sanger sequencing was performed using the Big Dye Terminator version 3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) and ITS1 or ITS4.

Article Title: Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya
Article Snippet: .. PCR products were purified for direct sequencing by enzymatic treatment using exonuclease I and shrimp alkaline phosphatase (PCR Product Presequencing Kit, Amersham). .. All DNA sequencing was carried out by direct cycle sequencing on both strands of purified PCR DNA products from PCR amplification.

Article Title: Novel TCAP Mutation c.32C > A Causing Limb Girdle Muscular Dystrophy 2G
Article Snippet: .. PCR products were purified by treating with exonuclease I and Shrimp alkaline phosphatase (Amersham, Piscataway, NJ) at 37°C and 80°C for 15 minutes each. .. Sequencing of PCR products was carried out using 100.0 ng (2.0 µl) of PCR product and 4 pmol (1.0 µl) of primer (forward and reverse in separate reactions), and 4.0 µl of BigDye Terminator ready mixture (Applied Biosystem, Foster city, USA).

Article Title: Bias in effect size of systemic lupus erythematosus susceptibility loci across Europe: a case-control study
Article Snippet: Genotyping DNA samples were amplified in a multiplex PCR with the KAPA2G fast HotStart (Kapa Biosystems, Woburn, MA, USA) in a final volume of 10 μl (20 ng genomic DNA), using 3 mM MgCl2 and 0.2 μM each primer. .. Products were purified by Exo-SAP digestion with exonuclease I (Epicentre, Madison, WI, USA) and shrimp alkaline phosphatase (GE Healthcare, Barcelona, Spain).

Article Title: Human and Murine Clonal CD8+ T Cell Expansions Arise during Tuberculosis Because of TCR Selection
Article Snippet: Paragraph title: Single cell sorting and single cell PCR ... For TCR product sequencing, a total of 12 μl of the products from the second round of the nested PCR amplification was combined with 1.5 μl of 10X shrimp alkaline phosphatase reaction buffer (200 mM Tris-HCl (pH 8.0) and 100 mM MgCl2), 1 U of shrimp alkaline phosphatase (Amersham Biosciences), and 1 U of exonuclease I (New England Biolabs), and water to total 15 μl.

Article Title: Exposure to Polycyclic Aromatic Hydrocarbons Among Never Smokers in Golestan Province, Iran, an Area of High Incidence of Esophageal Cancer - a Cross-Sectional Study with Repeated Measurement of Urinary 1-OHPG in Two Seasons
Article Snippet: The samples were incubated at 37°C (45 min) with 4 μl Exo-SAP-IT (Amersham, Roosendaal, the Netherlands) to degrade deoxynucleotide triphosphates and PCR primers. .. Following SBE, the samples were incubated at 37°C (1 h) with 1 unit shrimp alkaline phosphatase (Amersham) to degrade the unincorporated dideoxynucleotide triphosphates.

Nested PCR:

Article Title: Human and Murine Clonal CD8+ T Cell Expansions Arise during Tuberculosis Because of TCR Selection
Article Snippet: .. For TCR product sequencing, a total of 12 μl of the products from the second round of the nested PCR amplification was combined with 1.5 μl of 10X shrimp alkaline phosphatase reaction buffer (200 mM Tris-HCl (pH 8.0) and 100 mM MgCl2), 1 U of shrimp alkaline phosphatase (Amersham Biosciences), and 1 U of exonuclease I (New England Biolabs), and water to total 15 μl. ..

Plasmid Preparation:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The plasmid constructed for conjugation in A. actinomycetemcomitans is based on the mobilizable plasmid pGP704 ( ). .. The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK).

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: The amplicon was purified from an agarose gel and inserted into the pGEM-T Easy vector (Promega), generating the pGEM-T Easy-MS2 plasmid. pGEM-T Easy-MS2 was subsequently digested with Not I, generating a fragment of 3,604 bp containing the MS2 genome, which was purified and filled in using the Klenow fragment of DNA polymerase (New England Biolabs, Herts, United Kingdom). pET-47b(+) (Novagen, Darmstadt, Germany) was digested with Xba I and Xho I enzymes (New England Biolabs) liberating a 255 bp fragment of the polylinker region. .. After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

Software:

Article Title: Demographic History of a Recent Invasion of House Mice on the Isolated Island of Gough
Article Snippet: PCR products were purified using Exonuclease I and Shrimp Alkaline Phosphatase (Amersham Biosciences). .. Sequences were visualized and edited using the Geneious software (Biomatters Ltd, Auckland, New Zealand).

Article Title: XPD Polymorphisms and Risk of Squamous Cell Carcinoma of the Head and Neck in a Korean Sample
Article Snippet: One unit of shrimp alkaline phosphatase (Amersham Life Science, Cleveland, OH, USA) was added to the reaction mixture to clean up the primer extension reaction products, and the mixture was incubated at 37℃ for one hour, followed by 15 min at 72℃ for enzyme inactivation. .. Electrophoresis was performed on an ABI Prism 3100 Genetic Analyzer (Applied Biosystems) and the genes were analyzed using ABI Prism GeneScan and Genotyper software.

Article Title: Exposure to Polycyclic Aromatic Hydrocarbons Among Never Smokers in Golestan Province, Iran, an Area of High Incidence of Esophageal Cancer - a Cross-Sectional Study with Repeated Measurement of Urinary 1-OHPG in Two Seasons
Article Snippet: Genotyping was performed by single base extension (SBE) method using SnaPShot (Applied Biosystems, Nieuwerkerk, a.d. IJssel, the Netherlands), and primer 3 and Netprimer software were used to design SBE primers. .. Following SBE, the samples were incubated at 37°C (1 h) with 1 unit shrimp alkaline phosphatase (Amersham) to degrade the unincorporated dideoxynucleotide triphosphates.

Multiplex Assay:

Article Title: Root-associated fungal communities in three Pyroleae species and their mycobiont sharing with surrounding trees in subalpine coniferous forests on Mount Fuji, Japan
Article Snippet: The internal transcribed spacer (ITS) regions of fungal rDNA were amplified by polymerase chain reaction (PCR) using the forward primer ITS1F and the reverse primers ITS4, LR21, LR22, and LBW with a Multiplex PCR kit (QIAGEN, Hilden, Germany), following the manufacturer’s instructions. .. Amplification products were purified using a PCR clean-up kit containing exonuclease I and shrimp alkaline phosphatase (GE Healthcare, Hertfordshire, UK).

Article Title: Bias in effect size of systemic lupus erythematosus susceptibility loci across Europe: a case-control study
Article Snippet: Genotyping DNA samples were amplified in a multiplex PCR with the KAPA2G fast HotStart (Kapa Biosystems, Woburn, MA, USA) in a final volume of 10 μl (20 ng genomic DNA), using 3 mM MgCl2 and 0.2 μM each primer. .. Products were purified by Exo-SAP digestion with exonuclease I (Epicentre, Madison, WI, USA) and shrimp alkaline phosphatase (GE Healthcare, Barcelona, Spain).

Article Title: Exposure to Polycyclic Aromatic Hydrocarbons Among Never Smokers in Golestan Province, Iran, an Area of High Incidence of Esophageal Cancer - a Cross-Sectional Study with Repeated Measurement of Urinary 1-OHPG in Two Seasons
Article Snippet: Following SBE, the samples were incubated at 37°C (1 h) with 1 unit shrimp alkaline phosphatase (Amersham) to degrade the unincorporated dideoxynucleotide triphosphates. .. Two multiplex genotyping experiments (as described above) were used for SBE reactions.

Positron Emission Tomography:

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: The amplicon was purified from an agarose gel and inserted into the pGEM-T Easy vector (Promega), generating the pGEM-T Easy-MS2 plasmid. pGEM-T Easy-MS2 was subsequently digested with Not I, generating a fragment of 3,604 bp containing the MS2 genome, which was purified and filled in using the Klenow fragment of DNA polymerase (New England Biolabs, Herts, United Kingdom). pET-47b(+) (Novagen, Darmstadt, Germany) was digested with Xba I and Xho I enzymes (New England Biolabs) liberating a 255 bp fragment of the polylinker region. .. After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

Agarose Gel Electrophoresis:

Article Title: Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya
Article Snippet: The PCR was completed with a final extension step of 72°C for 9 min. PCR products showing successful amplification on agarose gel analysis were directly sequenced for both strands. .. PCR products were purified for direct sequencing by enzymatic treatment using exonuclease I and shrimp alkaline phosphatase (PCR Product Presequencing Kit, Amersham).

Article Title: Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
Article Snippet: The amplicon was purified from an agarose gel and inserted into the pGEM-T Easy vector (Promega), generating the pGEM-T Easy-MS2 plasmid. pGEM-T Easy-MS2 was subsequently digested with Not I, generating a fragment of 3,604 bp containing the MS2 genome, which was purified and filled in using the Klenow fragment of DNA polymerase (New England Biolabs, Herts, United Kingdom). pET-47b(+) (Novagen, Darmstadt, Germany) was digested with Xba I and Xho I enzymes (New England Biolabs) liberating a 255 bp fragment of the polylinker region. .. After purification, the ends were filled in by the Klenow fragment of DNA polymerase I and dephosphorylated with shrimp alkaline phosphatase (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

Concentration Assay:

Article Title: Demographic History of a Recent Invasion of House Mice on the Isolated Island of Gough
Article Snippet: DNA concentration was adjusted to 5ng/ul for all samples to ensure consistent PCR amplification and signal strength. .. PCR products were purified using Exonuclease I and Shrimp Alkaline Phosphatase (Amersham Biosciences).

Article Title: Human and Murine Clonal CD8+ T Cell Expansions Arise during Tuberculosis Because of TCR Selection
Article Snippet: For the second round of the nested reaction, 2 μL of the first reaction were combined with 18 μL of Taq buffer (50 mM KCl, 10 mM Tris-HCl (pH 8.3), and 2.5 mM MgCl2), 500 μM dNTP, 0.6 U of Taq polymerase, 50 μM of TRACint - or TRBCint -specific primer for the constant region and an oligonucleotide mixture of 23 TRAVint or 19 TRBVint primers (each 50 μM final concentration). .. For TCR product sequencing, a total of 12 μl of the products from the second round of the nested PCR amplification was combined with 1.5 μl of 10X shrimp alkaline phosphatase reaction buffer (200 mM Tris-HCl (pH 8.0) and 100 mM MgCl2), 1 U of shrimp alkaline phosphatase (Amersham Biosciences), and 1 U of exonuclease I (New England Biolabs), and water to total 15 μl.

DNA Purification:

Article Title: XPD Polymorphisms and Risk of Squamous Cell Carcinoma of the Head and Neck in a Korean Sample
Article Snippet: Genotyping We extracted the DNA from the peripheral blood using the Wizard™ Genomic DNA purification kit (Promega, Madison, WI, USA). .. One unit of shrimp alkaline phosphatase (Amersham Life Science, Cleveland, OH, USA) was added to the reaction mixture to clean up the primer extension reaction products, and the mixture was incubated at 37℃ for one hour, followed by 15 min at 72℃ for enzyme inactivation.

Marker:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: The kanamycin resistance gene from pUC-4k (Pharmacia, Kalamazoo, MI) was used as a selective marker in place of β-lactamase. .. The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK).

FACS:

Article Title: Human and Murine Clonal CD8+ T Cell Expansions Arise during Tuberculosis Because of TCR Selection
Article Snippet: Paragraph title: Single cell sorting and single cell PCR ... For TCR product sequencing, a total of 12 μl of the products from the second round of the nested PCR amplification was combined with 1.5 μl of 10X shrimp alkaline phosphatase reaction buffer (200 mM Tris-HCl (pH 8.0) and 100 mM MgCl2), 1 U of shrimp alkaline phosphatase (Amersham Biosciences), and 1 U of exonuclease I (New England Biolabs), and water to total 15 μl.

Homologous Recombination:

Article Title: The conserved carboxyl domain of MorC, an inner membrane protein of Aggregatibacter actinomycetemcomitans, is essential for membrane function
Article Snippet: Therefore, it was necessary to develop a morC deletion strain of A. actinomycetemcomitans to eliminate homologous recombination of the truncated gene. .. The cassette was ligated with pGP704 that had been digested with EcoRV and treated with shrimp alkaline phosphatase (Amersham Life Sciences, Buckinghamshire, UK).

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