shrimp alkaline phosphatase affymetrics  (New England Biolabs)


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  • 99
    Name:
    Shrimp Alkaline Phosphatase rSAP
    Description:
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    Catalog Number:
    m0371l
    Price:
    248
    Size:
    2 500 units
    Category:
    Alkaline Phosphatases
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    Structured Review

    New England Biolabs shrimp alkaline phosphatase affymetrics
    Shrimp Alkaline Phosphatase rSAP
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    https://www.bioz.com/result/shrimp alkaline phosphatase affymetrics/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phosphatase affymetrics - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Efficient Formation of the Tandem Thymine Glycol/8-oxo-7,8-dihydroguanine Lesion in Isolated DNA and the Mutagenic and Cytotoxic Properties of the Tandem Lesions in Escherichia coli Cells
    Article Snippet: .. For the bypass efficiency assay, 5% of the above PCR products was treated with 10 U NcoI and 1 U shrimp alkaline phosphatase in a 10-μL NEB buffer 2 at 37°C for 2 hrs, followed by heating at 65 °C for 20 min to deactivate the phosphatase. .. The mixture was then treated in a 15-μL NEB buffer 2 containing 5 mM DTT, ATP (50 pmol cold, premixed with 1.66 pmol [γ-32 P] ATP) and 10 U polynucleotide kinase.

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: .. We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India). .. All sequencing reactions were performed on ABI3100xl Automated system (Bangalore, India).

    Article Title: DMSO increases mutation-scanning detection sensitivity in clinical samples using high resolution melting
    Article Snippet: .. The PCR products were digested by Exonuclease I and Shrimp Alkaline Phosphatase (New England Biolabs) and processed for Sanger sequencing at Eton Bioscience Inc. (Boston, MA, USA). .. To enable sequencing of short PCR amplicons, a 30-T tail was added to the 5′-end of the forward primer ( ).

    Article Title: Potential tick vectors for Theileria equi in Israel.
    Article Snippet: .. Theileria equi Mehlhorn and Schein, 1998 (Piroplasmida: Babesiidae) is an important tick-borne pathogen of horses that is highly endemic in many parts of the world, including Israel. .. Theileria equi Mehlhorn and Schein, 1998 (Piroplasmida: Babesiidae) is an important tick-borne pathogen of horses that is highly endemic in many parts of the world, including Israel.

    Article Title: Multiple Divergent Haplotypes Express Completely Distinct Sets of Class I MHC Genes in Zebrafish
    Article Snippet: .. Prior to sequencing, PCR reactions (5 μL aliquots) were first mixed with 3 units of Exonuclease (Affymetrix) and 0.3 units of Shrimp Alkaline Phosphatase (NEB) and incubated at 37°C for 30 minutes followed by inactivation at 80°C for 15 minutes. .. Sequences were analyzed using SeqMan (DNAstar) to identify specific SNPs and assign haplotypes ( ).

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: .. For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase. .. The above mixture was then treated in 15 μl of New England Biolabs buffer 3 with 5 m m DTT, ATP (50 pmol of cold, premixed with 1.66 pmol of [γ-32 P]ATP), and 5 units of T4 polynucleotide kinase.

    Sequencing:

    Article Title: DMSO increases mutation-scanning detection sensitivity in clinical samples using high resolution melting
    Article Snippet: .. The PCR products were digested by Exonuclease I and Shrimp Alkaline Phosphatase (New England Biolabs) and processed for Sanger sequencing at Eton Bioscience Inc. (Boston, MA, USA). .. To enable sequencing of short PCR amplicons, a 30-T tail was added to the 5′-end of the forward primer ( ).

    Article Title: Potential tick vectors for Theileria equi in Israel.
    Article Snippet: .. Theileria equi Mehlhorn and Schein, 1998 (Piroplasmida: Babesiidae) is an important tick-borne pathogen of horses that is highly endemic in many parts of the world, including Israel. .. Theileria equi Mehlhorn and Schein, 1998 (Piroplasmida: Babesiidae) is an important tick-borne pathogen of horses that is highly endemic in many parts of the world, including Israel.

    Article Title: Multiple Divergent Haplotypes Express Completely Distinct Sets of Class I MHC Genes in Zebrafish
    Article Snippet: .. Prior to sequencing, PCR reactions (5 μL aliquots) were first mixed with 3 units of Exonuclease (Affymetrix) and 0.3 units of Shrimp Alkaline Phosphatase (NEB) and incubated at 37°C for 30 minutes followed by inactivation at 80°C for 15 minutes. .. Sequences were analyzed using SeqMan (DNAstar) to identify specific SNPs and assign haplotypes ( ).

    Incubation:

    Article Title: Multiple Divergent Haplotypes Express Completely Distinct Sets of Class I MHC Genes in Zebrafish
    Article Snippet: .. Prior to sequencing, PCR reactions (5 μL aliquots) were first mixed with 3 units of Exonuclease (Affymetrix) and 0.3 units of Shrimp Alkaline Phosphatase (NEB) and incubated at 37°C for 30 minutes followed by inactivation at 80°C for 15 minutes. .. Sequences were analyzed using SeqMan (DNAstar) to identify specific SNPs and assign haplotypes ( ).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Translesion Synthesis of 8,5′-Cyclopurine-2′-deoxynucleosides by DNA Polymerases η, ι, and ζ *
    Article Snippet: .. For PAGE analysis, a portion of the PCR fragments was treated with 5 units of NcoI and 1 unit of shrimp alkaline phosphatase at 37 °C in 10 μl of New England Biolab buffer 3 for 1 h, followed by heating at 80 °C for 20 min to deactivate the shrimp alkaline phosphatase. .. The above mixture was then treated in 15 μl of New England Biolabs buffer 3 with 5 m m DTT, ATP (50 pmol of cold, premixed with 1.66 pmol of [γ-32 P]ATP), and 5 units of T4 polynucleotide kinase.

    Purification:

    Article Title: Commensalism facilitates gene flow in mountains: a comparison between two Rattus species
    Article Snippet: .. We purified all PCR products by the single-step enzymatic process with exonuclease 1 and shrimp alkaline phosphatase (from New England Biolabs, Bangalore, India). .. All sequencing reactions were performed on ABI3100xl Automated system (Bangalore, India).

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  • 99
    New England Biolabs exonuclease i
    Exonuclease I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease i/product/New England Biolabs
    Average 99 stars, based on 561 article reviews
    Price from $9.99 to $1999.99
    exonuclease i - by Bioz Stars, 2020-07
    99/100 stars
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