sheep anti cathepsin h  (Millipore)


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    Structured Review

    Millipore sheep anti cathepsin h
    Expression of cathepsins C, H and L in TALL-104 cells and human CD8+ T cells and their co-localisation with cystatin F. (A-C) TALL-104 and pCTLs were analysed for cathepsins C, H and L expression by western blot. GAPDH or β-actin staining was used to show protein loading. (D, E) Co-localisation of cystatin F with cathepsins C, H and L was studied by immunofluorescence microscopy in TALL-104 (D) and pCTLs (E) . Cystatin F (green) and cathepsin C (red) co-localisation is shown in first row, cystatin F (red) and <t>cathepsin</t> H (green) in second row and cystatin F (red) and cathepsin L (green) in third row. Bars represent 10 μm. (F) TALL-104 cell lysates were immunoprecipitated with cystatin F antibody and analysed by western blot with anti-cathepsin C and H antibodies. (G) Proximity ligation experiment for cystatin F-cathepsin C interaction in TALL-104 cells and pCTLs. Signals were quantified in BlobFinder software. Bars represent 10 μm. ctrl = control; cysF = cystatin F; IP = immunoprecipitation; pCTL = primary human cytotoxic T cells
    Sheep Anti Cathepsin H, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep anti cathepsin h/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sheep anti cathepsin h - by Bioz Stars, 2020-05
    93/100 stars

    Images

    1) Product Images from "Increased Cystatin F Levels Correlate with Decreased Cytotoxicity of Cytotoxic T Cells"

    Article Title: Increased Cystatin F Levels Correlate with Decreased Cytotoxicity of Cytotoxic T Cells

    Journal: Radiology and Oncology

    doi: 10.2478/raon-2019-0007

    Expression of cathepsins C, H and L in TALL-104 cells and human CD8+ T cells and their co-localisation with cystatin F. (A-C) TALL-104 and pCTLs were analysed for cathepsins C, H and L expression by western blot. GAPDH or β-actin staining was used to show protein loading. (D, E) Co-localisation of cystatin F with cathepsins C, H and L was studied by immunofluorescence microscopy in TALL-104 (D) and pCTLs (E) . Cystatin F (green) and cathepsin C (red) co-localisation is shown in first row, cystatin F (red) and cathepsin H (green) in second row and cystatin F (red) and cathepsin L (green) in third row. Bars represent 10 μm. (F) TALL-104 cell lysates were immunoprecipitated with cystatin F antibody and analysed by western blot with anti-cathepsin C and H antibodies. (G) Proximity ligation experiment for cystatin F-cathepsin C interaction in TALL-104 cells and pCTLs. Signals were quantified in BlobFinder software. Bars represent 10 μm. ctrl = control; cysF = cystatin F; IP = immunoprecipitation; pCTL = primary human cytotoxic T cells
    Figure Legend Snippet: Expression of cathepsins C, H and L in TALL-104 cells and human CD8+ T cells and their co-localisation with cystatin F. (A-C) TALL-104 and pCTLs were analysed for cathepsins C, H and L expression by western blot. GAPDH or β-actin staining was used to show protein loading. (D, E) Co-localisation of cystatin F with cathepsins C, H and L was studied by immunofluorescence microscopy in TALL-104 (D) and pCTLs (E) . Cystatin F (green) and cathepsin C (red) co-localisation is shown in first row, cystatin F (red) and cathepsin H (green) in second row and cystatin F (red) and cathepsin L (green) in third row. Bars represent 10 μm. (F) TALL-104 cell lysates were immunoprecipitated with cystatin F antibody and analysed by western blot with anti-cathepsin C and H antibodies. (G) Proximity ligation experiment for cystatin F-cathepsin C interaction in TALL-104 cells and pCTLs. Signals were quantified in BlobFinder software. Bars represent 10 μm. ctrl = control; cysF = cystatin F; IP = immunoprecipitation; pCTL = primary human cytotoxic T cells

    Techniques Used: Expressing, Western Blot, Staining, Immunofluorescence, Microscopy, Immunoprecipitation, Ligation, Software

    Related Articles

    Chromatography:

    Article Title: Evidence for efficient phosphorylation of EGFR and rapid endocytosis of phosphorylated EGFR via the early/late endocytic pathway in a gefitinib-sensitive non-small cell lung cancer cell line
    Article Snippet: .. Anti-cathepsin D or anti-LIMPII IgG was affinity-purified by protein A Sepharose CL-4B (Sigma), followed by immunoaffinity chromatography using antigen-conjugated Sepharose 4B. .. A mouse monoclonal anti-pEGFR was obtained from DakoCytomation (Denmark) and BD Biosciences (San Jose, CA, USA).

    Blocking Assay:

    Article Title: Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells
    Article Snippet: .. After transfer to nitrocellulose (NC) membranes (Biometra, Goettingen, Germany) and blocking with 5% BSA or dry milk, the fractions were analyzed for subcellular marker proteins with the following antibodies: anti-FasL clone G-247.4 (BD Biosciences), anti-CD63 clone MEM-259 (Acris Antibodies, Herford, Germany), anti-LAMP-1 clone 25 (BD Biosciences), anti-cathepsin D clone CTD-19 (Sigma), anti-cytochrome oxidase IV (CoxIV) mAb clone 10G8D12C12 (1/1000, MitoScience, Eugene, OR, USA), anti pan-cadherin clone ab22744 (Abcam, Cambridge, UK) and horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (GE Healthcare, Munich, Germany). ..

    Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
    Article Snippet: .. After blocking, samples were incubated with anti-CD63 antibody (BD Biosciences), anti-cathepsin D antibody (Sigma), or chemokine anti-C-X-C motif ligand 10 (CXCL10; Abcam) for 1 h. Samples were then washed 3 times with PBS and incubated with Alexa Fluor-conjugated secondary antibodies for 1 h at RT (Alexa Fluor 488, 594, or 647; Invitrogen). .. Images were visualized by fluorescence microscopy using a Ti-U microscope with a 60× oil immersion objective and D5-QilMc digital camera (Nikon).

    Incubation:

    Article Title: Infection of Primary Human Alveolar Macrophages Alters Staphylococcus aureus Toxin Production and Activity
    Article Snippet: .. After blocking, samples were incubated with anti-CD63 antibody (BD Biosciences), anti-cathepsin D antibody (Sigma), or chemokine anti-C-X-C motif ligand 10 (CXCL10; Abcam) for 1 h. Samples were then washed 3 times with PBS and incubated with Alexa Fluor-conjugated secondary antibodies for 1 h at RT (Alexa Fluor 488, 594, or 647; Invitrogen). .. Images were visualized by fluorescence microscopy using a Ti-U microscope with a 60× oil immersion objective and D5-QilMc digital camera (Nikon).

    other:

    Article Title: Rac deletion in osteoclasts causes severe osteopetrosis
    Article Snippet: Mouse anti-Rac1 (clone 23a8), rabbit anti-Rac2 and mouse anti-cathepsin-K antibodies were from Millipore.

    Double Immunofluorescence Staining:

    Article Title: Selective Cathepsin S Inhibition Attenuates Atherosclerosis in Apolipoprotein E–Deficient Mice with Chronic Renal Disease
    Article Snippet: .. Double immunofluorescence staining was performed using cathepsin S antibodies and either mac3 or α-smooth muscle actin (clone 1A4; Sigma-Aldrich). .. Sections were counterstained with DAPI to visualize nuclei.

    Marker:

    Article Title: Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells
    Article Snippet: .. After transfer to nitrocellulose (NC) membranes (Biometra, Goettingen, Germany) and blocking with 5% BSA or dry milk, the fractions were analyzed for subcellular marker proteins with the following antibodies: anti-FasL clone G-247.4 (BD Biosciences), anti-CD63 clone MEM-259 (Acris Antibodies, Herford, Germany), anti-LAMP-1 clone 25 (BD Biosciences), anti-cathepsin D clone CTD-19 (Sigma), anti-cytochrome oxidase IV (CoxIV) mAb clone 10G8D12C12 (1/1000, MitoScience, Eugene, OR, USA), anti pan-cadherin clone ab22744 (Abcam, Cambridge, UK) and horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (GE Healthcare, Munich, Germany). ..

    Staining:

    Article Title: Phospholipases C and A2 control lysosome-mediated IL-1? secretion: Implications for inflammatory processes
    Article Snippet: .. Filters were stained with anti-cathepsin D mAb (IgG2a, Calbiochem), anti-IL-1β mAb (3ZD, IgG1, obtained from the National Cancer Institute Biological Resources Branch), or rabbit anti-caspase-1 Ab (DBA Italia, Segrate, Milan) followed by a horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG (DAKO) and developed with ECL-plus (Amersham Biosciences). ..

    Affinity Purification:

    Article Title: Evidence for efficient phosphorylation of EGFR and rapid endocytosis of phosphorylated EGFR via the early/late endocytic pathway in a gefitinib-sensitive non-small cell lung cancer cell line
    Article Snippet: .. Anti-cathepsin D or anti-LIMPII IgG was affinity-purified by protein A Sepharose CL-4B (Sigma), followed by immunoaffinity chromatography using antigen-conjugated Sepharose 4B. .. A mouse monoclonal anti-pEGFR was obtained from DakoCytomation (Denmark) and BD Biosciences (San Jose, CA, USA).

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    Millipore sheep anti tyrosine hydroxylase
    Sheep Anti Tyrosine Hydroxylase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep anti tyrosine hydroxylase/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    sheep anti tyrosine hydroxylase - by Bioz Stars, 2020-05
    99/100 stars
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