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Sony sh800z cell sorter
C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
Sh800z Cell Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors"

Article Title: An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors

Journal: Scientific Reports

doi: 10.1038/srep35908

C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
Figure Legend Snippet: C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p

Techniques Used: Quantitative RT-PCR, Expressing

Related Articles

Fluorescence:

Article Title: Motility of glioblastoma cells is driven by netrin-1 induced gain of stemness
Article Snippet: The pLenti CMV GFP Puro plasmid was a gift from Eric Campeau (Addgene #17448) [ ]. .. Pool of GFP positive cells were then sorted with fluorescence activated cell sorting using Sony SH800Z Cell Sorter. .. Orthotopic glioblastoma xenografts All experimental procedures involving mice were authorized by the National Animal Experiment Board.

Article Title: Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation
Article Snippet: Quantification of NP perimeter, roundness, contact surface length, and angle toward UB epithelium was obtained from paraffin sections stained with CTNND1, CALB1, and Hoechst. .. Six2-TGC tg/+ -containing control (n = 34) and dko (n = 22) E13.5 kidneys were dissociated into single-cell suspensions with 0.25% trypsin, and GFP+ NP cells were isolated using fluorescence-activated cell sorting (FACS) with a Sony SH800Z cell sorter. .. Cells were subsequently fixed with 70% EtOH, treated with RNAse A (Thermo Fisher Scientific), and stained with propidium iodide (Invitrogen).

Article Title: Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation
Article Snippet: Measurement of Cellular Properties Quantification of NP perimeter, roundness, contact surface length, and angle toward UB epithelium was obtained from paraffin sections stained with CTNND1, CALB1, and Hoechst. .. Cell-Cycle Analysis Six2-TGC tg/+ -containing control (n = 34) and dko (n = 22) E13.5 kidneys were dissociated into single-cell suspensions with 0.25% trypsin, and GFP+ NP cells were isolated using fluorescence-activated cell sorting (FACS) with a Sony SH800Z cell sorter. .. Cells were subsequently fixed with 70% EtOH, treated with RNAse A (Thermo Fisher Scientific), and stained with propidium iodide (Invitrogen).

FACS:

Article Title: Motility of glioblastoma cells is driven by netrin-1 induced gain of stemness
Article Snippet: The pLenti CMV GFP Puro plasmid was a gift from Eric Campeau (Addgene #17448) [ ]. .. Pool of GFP positive cells were then sorted with fluorescence activated cell sorting using Sony SH800Z Cell Sorter. .. Orthotopic glioblastoma xenografts All experimental procedures involving mice were authorized by the National Animal Experiment Board.

Article Title: Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation
Article Snippet: Quantification of NP perimeter, roundness, contact surface length, and angle toward UB epithelium was obtained from paraffin sections stained with CTNND1, CALB1, and Hoechst. .. Six2-TGC tg/+ -containing control (n = 34) and dko (n = 22) E13.5 kidneys were dissociated into single-cell suspensions with 0.25% trypsin, and GFP+ NP cells were isolated using fluorescence-activated cell sorting (FACS) with a Sony SH800Z cell sorter. .. Cells were subsequently fixed with 70% EtOH, treated with RNAse A (Thermo Fisher Scientific), and stained with propidium iodide (Invitrogen).

Article Title: The Hidden Sexuality of Alexandrium Minutum: An Example of Overlooked Sex in Dinoflagellates
Article Snippet: .. Cell sorting: Cells at the different DNA content peaks ( > 200 cells per population) were sorted at low speed and in high purity mode in a SH800Z cell sorter (Sony Biotechnology Inc.) equipped with a 488 nm diode laser. ..

Article Title: Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation
Article Snippet: Measurement of Cellular Properties Quantification of NP perimeter, roundness, contact surface length, and angle toward UB epithelium was obtained from paraffin sections stained with CTNND1, CALB1, and Hoechst. .. Cell-Cycle Analysis Six2-TGC tg/+ -containing control (n = 34) and dko (n = 22) E13.5 kidneys were dissociated into single-cell suspensions with 0.25% trypsin, and GFP+ NP cells were isolated using fluorescence-activated cell sorting (FACS) with a Sony SH800Z cell sorter. .. Cells were subsequently fixed with 70% EtOH, treated with RNAse A (Thermo Fisher Scientific), and stained with propidium iodide (Invitrogen).

Isolation:

Article Title: Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation
Article Snippet: Quantification of NP perimeter, roundness, contact surface length, and angle toward UB epithelium was obtained from paraffin sections stained with CTNND1, CALB1, and Hoechst. .. Six2-TGC tg/+ -containing control (n = 34) and dko (n = 22) E13.5 kidneys were dissociated into single-cell suspensions with 0.25% trypsin, and GFP+ NP cells were isolated using fluorescence-activated cell sorting (FACS) with a Sony SH800Z cell sorter. .. Cells were subsequently fixed with 70% EtOH, treated with RNAse A (Thermo Fisher Scientific), and stained with propidium iodide (Invitrogen).

Article Title: Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation
Article Snippet: Measurement of Cellular Properties Quantification of NP perimeter, roundness, contact surface length, and angle toward UB epithelium was obtained from paraffin sections stained with CTNND1, CALB1, and Hoechst. .. Cell-Cycle Analysis Six2-TGC tg/+ -containing control (n = 34) and dko (n = 22) E13.5 kidneys were dissociated into single-cell suspensions with 0.25% trypsin, and GFP+ NP cells were isolated using fluorescence-activated cell sorting (FACS) with a Sony SH800Z cell sorter. .. Cells were subsequently fixed with 70% EtOH, treated with RNAse A (Thermo Fisher Scientific), and stained with propidium iodide (Invitrogen).

Expressing:

Article Title: Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the cell
Article Snippet: Cells were cotransfected with 0.5 μg of plasmid expressing Cas9-GFP (pX458-Addgene 48138) (for CENP-C tagging) or Cas9-mCherry (modified pX458 with GFP replaced with mCherry, a gift from the Rajat Rohatgi lab, Stanford University) (for CENP-N tagging) and guide RNA targeting sequence and 0.5 µg of homology arm donor constructs using Promega Fugene HD in a six-well plate well. .. Cells were sorted using a Sony SH800Z Cell sorter for Cas9 expression 2 d posttransfection. .. Sorted cells were outgrown to confluency and then sorted for single cells that expressed the endogenous fusion tags and counter selected for Cas9 expression.

Chromatin Immunoprecipitation:

Article Title: An adhesion code ensures robust pattern formation during tissue morphogenesis
Article Snippet: .. Cells were sorted using a Sony SH800Z cell sorter, with a 100 μm chip. ..

Cell Cycle Assay:

Article Title: Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation
Article Snippet: Measurement of Cellular Properties Quantification of NP perimeter, roundness, contact surface length, and angle toward UB epithelium was obtained from paraffin sections stained with CTNND1, CALB1, and Hoechst. .. Cell-Cycle Analysis Six2-TGC tg/+ -containing control (n = 34) and dko (n = 22) E13.5 kidneys were dissociated into single-cell suspensions with 0.25% trypsin, and GFP+ NP cells were isolated using fluorescence-activated cell sorting (FACS) with a Sony SH800Z cell sorter. .. Cells were subsequently fixed with 70% EtOH, treated with RNAse A (Thermo Fisher Scientific), and stained with propidium iodide (Invitrogen).

Clone Assay:

Article Title: Zbp1-positive cells are osteogenic progenitors in periodontal ligament
Article Snippet: The area analysis was performed by ImageJ software v1.53c (imagej.net). .. Cloning and screening of murine PDL cellsSingle-cell sorting was performed using the SH800Z Cell Sorter (Sony Biotechnology, CA, USA) with the 100-μm microfluidic sorting chips set on a single-cell mode. .. Cells were sorted into 96-well plates containing the cell culture medium (200 µL) with FGF-2.

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    Sony sh800z cell sorter
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Sh800z Cell Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh800z cell sorter/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sh800z cell sorter - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Sony sh800z sorter
    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an <t>SH800Z</t> cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p
    Sh800z Sorter, supplied by Sony, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh800z sorter/product/Sony
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sh800z sorter - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

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    C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p

    Journal: Scientific Reports

    Article Title: An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors

    doi: 10.1038/srep35908

    Figure Lengend Snippet: C8 augments important maturation markers specifically in INS-producing β cells. Quantitative RT-PCR analysis was performed to measure the relative expression levels of the β cell lineage markers INS , UCN3 , and GCK and the transcription factors PDX1 , NKX6.1 , and MAFA from sorted Venus+ and NC cells with or without treatment with C8 and/or bFGF. Briefly, #9–15 hiPSCs were differentiated as described above and treated with 5 μM C8 or 5 μM C8 and 50 ng/mL bFGF in the third stage of differentiation. After differentiation, samples were dissociated with TrypLE and sorted with an SH800Z cell sorter (Sony) for Venus+ and NC cells. Fifty cells from each group were sorted into 5 μL FCP reagent, which is a lysate reagent of the QIAGEN Fast Lane cDNA kit. For cDNA synthesis, 2 μL of the samples were used, with 0.5 μL of the 10 μL cDNA samples used for quantitative RT-PCR. Error bars indicate SD, n = 3. *p

    Article Snippet: The number of Venus- and mCherry-expressing cells was analysed in an SH800Z cell sorter (Sony, Tokyo, Japan).

    Techniques: Quantitative RT-PCR, Expressing