sg021 streptavidin horseradish peroxidase pierce  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sg021 streptavidin horseradish peroxidase pierce
    Sg021 Streptavidin Horseradish Peroxidase Pierce, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sg021 streptavidin horseradish peroxidase pierce/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sg021 streptavidin horseradish peroxidase pierce - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Kinase-Catalyzed Biotinylation
    Article Snippet: .. For Hela cell lysates, we used PKA kinase buffer supplied by the manufacturer, which is provided in the Reagents and Solutions section Protease inhibitor cocktail: Calbiochem, cat. No. 539137 β-mercaptoethanol: Fisher, cat. No. 75-36-5 PVDF membrane: Immobilon PSQ -Millipore, cat. No. IPVH00010 Coomassie Blue stain: NuSep, cat. No. SG021 Streptavidin-horseradish peroxidase: Pierce, cat. No EN-N200 Lysis buffer (see recipe) SDS loading buffer (see recipe) 12% SDS-polyacrylamide gel Mini-Protean electrophoretic system: BioRad Mini-Transblot cell: BioRad Gel imaging instrument: Molecular Dynamics Storm 8600 ..

    Cell Culture:

    Article Title: Kinase-Catalyzed Biotinylation
    Article Snippet: To use Hela cells (National Cell Culture Center, ), 10 million cells were lysed in lysis buffer (see recipe below). .. For Hela cell lysates, we used PKA kinase buffer supplied by the manufacturer, which is provided in the Reagents and Solutions section Protease inhibitor cocktail: Calbiochem, cat. No. 539137 β-mercaptoethanol: Fisher, cat. No. 75-36-5 PVDF membrane: Immobilon PSQ -Millipore, cat. No. IPVH00010 Coomassie Blue stain: NuSep, cat. No. SG021 Streptavidin-horseradish peroxidase: Pierce, cat. No EN-N200 Lysis buffer (see recipe) SDS loading buffer (see recipe) 12% SDS-polyacrylamide gel Mini-Protean electrophoretic system: BioRad Mini-Transblot cell: BioRad Gel imaging instrument: Molecular Dynamics Storm 8600

    Labeling:

    Article Title: Kinase-Catalyzed Biotinylation
    Article Snippet: ATP-biotin (Adenosine 5′-triphosphate [γ]-biotinyl-3, 6, 9-trioxaundecanediamine, Affinity Labeling Technologies, cat. no. 864538-90-9, 10 mM stored in methanol) Cells lysates stock solution: typically the stock solution is approximately 10 mg/mL, which will be diluted to a final concentration of 1–5 mg/mL in the reaction, depending on the requirement of the reaction. .. For Hela cell lysates, we used PKA kinase buffer supplied by the manufacturer, which is provided in the Reagents and Solutions section Protease inhibitor cocktail: Calbiochem, cat. No. 539137 β-mercaptoethanol: Fisher, cat. No. 75-36-5 PVDF membrane: Immobilon PSQ -Millipore, cat. No. IPVH00010 Coomassie Blue stain: NuSep, cat. No. SG021 Streptavidin-horseradish peroxidase: Pierce, cat. No EN-N200 Lysis buffer (see recipe) SDS loading buffer (see recipe) 12% SDS-polyacrylamide gel Mini-Protean electrophoretic system: BioRad Mini-Transblot cell: BioRad Gel imaging instrument: Molecular Dynamics Storm 8600

    Concentration Assay:

    Article Title: Kinase-Catalyzed Biotinylation
    Article Snippet: ATP-biotin (Adenosine 5′-triphosphate [γ]-biotinyl-3, 6, 9-trioxaundecanediamine, Affinity Labeling Technologies, cat. no. 864538-90-9, 10 mM stored in methanol) Cells lysates stock solution: typically the stock solution is approximately 10 mg/mL, which will be diluted to a final concentration of 1–5 mg/mL in the reaction, depending on the requirement of the reaction. .. For Hela cell lysates, we used PKA kinase buffer supplied by the manufacturer, which is provided in the Reagents and Solutions section Protease inhibitor cocktail: Calbiochem, cat. No. 539137 β-mercaptoethanol: Fisher, cat. No. 75-36-5 PVDF membrane: Immobilon PSQ -Millipore, cat. No. IPVH00010 Coomassie Blue stain: NuSep, cat. No. SG021 Streptavidin-horseradish peroxidase: Pierce, cat. No EN-N200 Lysis buffer (see recipe) SDS loading buffer (see recipe) 12% SDS-polyacrylamide gel Mini-Protean electrophoretic system: BioRad Mini-Transblot cell: BioRad Gel imaging instrument: Molecular Dynamics Storm 8600

    Imaging:

    Article Title: Kinase-Catalyzed Biotinylation
    Article Snippet: .. For Hela cell lysates, we used PKA kinase buffer supplied by the manufacturer, which is provided in the Reagents and Solutions section Protease inhibitor cocktail: Calbiochem, cat. No. 539137 β-mercaptoethanol: Fisher, cat. No. 75-36-5 PVDF membrane: Immobilon PSQ -Millipore, cat. No. IPVH00010 Coomassie Blue stain: NuSep, cat. No. SG021 Streptavidin-horseradish peroxidase: Pierce, cat. No EN-N200 Lysis buffer (see recipe) SDS loading buffer (see recipe) 12% SDS-polyacrylamide gel Mini-Protean electrophoretic system: BioRad Mini-Transblot cell: BioRad Gel imaging instrument: Molecular Dynamics Storm 8600 ..

    Lysis:

    Article Title: Kinase-Catalyzed Biotinylation
    Article Snippet: .. For Hela cell lysates, we used PKA kinase buffer supplied by the manufacturer, which is provided in the Reagents and Solutions section Protease inhibitor cocktail: Calbiochem, cat. No. 539137 β-mercaptoethanol: Fisher, cat. No. 75-36-5 PVDF membrane: Immobilon PSQ -Millipore, cat. No. IPVH00010 Coomassie Blue stain: NuSep, cat. No. SG021 Streptavidin-horseradish peroxidase: Pierce, cat. No EN-N200 Lysis buffer (see recipe) SDS loading buffer (see recipe) 12% SDS-polyacrylamide gel Mini-Protean electrophoretic system: BioRad Mini-Transblot cell: BioRad Gel imaging instrument: Molecular Dynamics Storm 8600 ..

    Staining:

    Article Title: Kinase-Catalyzed Biotinylation
    Article Snippet: .. For Hela cell lysates, we used PKA kinase buffer supplied by the manufacturer, which is provided in the Reagents and Solutions section Protease inhibitor cocktail: Calbiochem, cat. No. 539137 β-mercaptoethanol: Fisher, cat. No. 75-36-5 PVDF membrane: Immobilon PSQ -Millipore, cat. No. IPVH00010 Coomassie Blue stain: NuSep, cat. No. SG021 Streptavidin-horseradish peroxidase: Pierce, cat. No EN-N200 Lysis buffer (see recipe) SDS loading buffer (see recipe) 12% SDS-polyacrylamide gel Mini-Protean electrophoretic system: BioRad Mini-Transblot cell: BioRad Gel imaging instrument: Molecular Dynamics Storm 8600 ..