sfaai asisi 10 u µl  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sfaai asisi 10 u µl
    Sfaai Asisi 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sfaai asisi 10 u µl/product/Thermo Fisher
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sfaai asisi 10 u µl - by Bioz Stars, 2020-01
    81/100 stars

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    Clone Assay:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871). .. The MYC-GFP coding sequence was amplified from pTRIPZ-del89-c-Myc-d4EGFP using the primers 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGCCCCTCAACGTTAGCTTC-3′ and 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTTCTACACATTGATCCTAGCAG-3′.

    Amplification:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871).

    Construct:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871). .. The larger fragment was ligated with a PscI- and SfaAIdigested PCR product obtained from amplification of pTRIPZ using the primers 5′-TTTGACCTTGACATGCT- 3′ and 5′-ACTTATGCGATCGCGCCCCAGCTGGTTCT-3′, resulting in plasmids pTRIPZ-del89-c-Myc-d4EGFP and pTRIPZ-del89-d4EGFP for MYC-GFP and GFP expression, respectively.

    Polymerase Chain Reaction:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871).

    Expressing:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871).

    Sequencing:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871). .. A separate smaller doxycycline-inducible MYC-GFP expression plasmid was constructed for integration into hTERT-RPE1 cells.

    Plasmid Preparation:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871). .. The larger fragment was ligated with a PscI- and SfaAIdigested PCR product obtained from amplification of pTRIPZ using the primers 5′-TTTGACCTTGACATGCT- 3′ and 5′-ACTTATGCGATCGCGCCCCAGCTGGTTCT-3′, resulting in plasmids pTRIPZ-del89-c-Myc-d4EGFP and pTRIPZ-del89-d4EGFP for MYC-GFP and GFP expression, respectively.

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  • News
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  • 81
    Thermo Fisher sfaai asisi 10 u µl
    Sfaai Asisi 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sfaai asisi 10 u µl/product/Thermo Fisher
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sfaai asisi 10 u µl - by Bioz Stars, 2020-01
    81/100 stars
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