Journal: Cell Reports Medicine
Article Title: Pyroptotic T cell-derived active IL-16 has a driving function in ovarian endometriosis development
doi: 10.1016/j.xcrm.2024.101476
Figure Lengend Snippet: Active IL-16 promotes the production of IL-1β, IL-6, IL-8, and TNF (A) Correlation analysis between IL-16 and IL-6 using the Spearman correlation method. (B) Correlation analysis between IL-16 and IL-1β using the Spearman correlation method. (C) Representative analysis of IL-6 expression by CD45 + CD56 − CD3 + CD4 + T cells isolated from the RNE and EE. (D) Statistical analysis of the percentages of IL-6-producing T cells among the CD4 + T cell population isolated from the RNE (n = 9) and EE (n = 10), unpaired t test. Data represent a pool from multiple independent experiments. (E, G, and I) PBMCs from a healthy donor were cultured with or without active IL-16 (10 or 100 ng/mL). For the detection of TNF, each group of cells was treated with phorbol myristate acetate (50 ng/mL), ionomycin (1 μg/mL), and monensin (10 μg/mL). After 4 h, the expression of IL-6, TNF, and TGF-β1 was determined by intracellular staining. (F, H, and J) Statistical analysis of the percentages of IL-6 + , TNF + , and TGF-β1 + cells among the CD4 + T cell population, paired t test, IL-6 (n = 14), TNF (n = 9), and TGF-β1 (n = 9). Data in (E)–(J) represent a pool from multiple independent experiments. (K‒O) PBMCs from a healthy donor were stimulated for 12 h in the presence of PBS or different concentrations of active IL-16 (10 or 100 ng/mL), after which the production of IL-6, IL-1β, TNF, CXCL8, and TGF-β in cell supernatants was analyzed using a CBA assay, paired t test, n = 21. (P) PBMCs from a healthy donor were stimulated for 12 h in the presence of PBS or different concentrations of IL-6 (10 or 100 ng/mL), after which the production of IL-16 in cell supernatants was analyzed using ELISA, paired t test, n = 10. Data in (K)–(P) represent a pool from multiple independent experiments.
Article Snippet: Hu IL-8 CBA Flex Set A9 100Tst , BD , 558277.
Techniques: Expressing, Isolation, Cell Culture, Staining, Enzyme-linked Immunosorbent Assay
