sequencing grade modified trypsin  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    Sequencing Grade Modified Trypsin
    Description:
    Cleavage sites C terminal of Arg and Lys Optimal pH 7 9
    Catalog Number:
    v5111
    Price:
    None
    Category:
    Protein Analysis Mass Spectrometry Proteases and Surfactants
    Buy from Supplier


    Structured Review

    Promega sequencing grade modified trypsin
    Cleavage sites C terminal of Arg and Lys Optimal pH 7 9
    https://www.bioz.com/result/sequencing grade modified trypsin/product/Promega
    Average 90 stars, based on 136 article reviews
    Price from $9.99 to $1999.99
    sequencing grade modified trypsin - by Bioz Stars, 2020-01
    90/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Isolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from Capra hircus ear cartilage
    Article Snippet: The dehydrated gel pieces were digested with sequence graded trypsin at 37 °C air bath for overnight (Product number V5111, Promega gold, Madison, WI) to cleave the protein at specific peptide bonds depending on the amino-acid sequences . .. To stop the trypsin reaction, peptide extraction was performed by addition of 50 µl of extraction solution (60% acetonitrile, 0.1% TFA) to gel pieces thrice followed by centrifugation at 12,000 × g for 20 s. Supernant was collected and lyophilized for further use.

    Article Title: Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches
    Article Snippet: Proteins were recovered by centrifugation (13.2x103 rpm, 15 minutes, 4°C), and 5μg of proteins (Bradford, 1976) were submitted to a label-free differential proteomic analysis. .. Proteins were precipitated with acetone overnight, and then dissolved in 40μl of 50mM NH4 HCO3 (v/v, pH = 8.5) containing 1μg of trypsine (Promega V51 11).

    Article Title: Proteomic characterization of serine hydrolase activity and composition in normal urine
    Article Snippet: Affinity purification & identification of active serine hydrolases Normal urine (50 mL) was concentrated to 800 μL using centrifugation (4000 g, 30 minutes, 4°C) with a 30 kDa cut-off [Amicon® UFC903024]. .. For in-gel digestion, tagged proteins were separated on LDS-PAGE, bands excised from gels and analyzed by MS-MS. For in-solution digestion, the Dynabeads® were washed (50 mM PBS, 0.25% Tween 20), and tagged proteins digested with 250 ng trypsin [Promega V5111].

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: The reaction was cleared by centrifugation then incubated with protein A beads (20 μl/IP) for 1 hr. .. Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Electrophoresis:

    Article Title: Isolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from Capra hircus ear cartilage
    Article Snippet: Processing of protein bands for in-gel digestion: After SDS-PAGE electrophoresis, the required protein band was excised (1–2 mm) and digested with sequence graded trypsin according to the protocol published elswhere . .. The dehydrated gel pieces were digested with sequence graded trypsin at 37 °C air bath for overnight (Product number V5111, Promega gold, Madison, WI) to cleave the protein at specific peptide bonds depending on the amino-acid sequences .

    Incubation:

    Article Title: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
    Article Snippet: .. After destaining and alkylation, each piece of gel was incubated with trypsin (Promega, http://worldwide.promega.com/search-results/?q=V5111 ) and extracted as described in. .. Resulting peptides were injected to LC-MS/MS system (RSLCnano connected on line to Orbitrap Elite; Thermo Fisher Scientific).

    Article Title: Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes
    Article Snippet: The reduced and alkylated proteins were then precipitated using chilled acetone (1:5 v/v of sample) and incubated at −80 °C for 4–6 h to ensure complete removal of SDS from sample. .. Trypsin digestion was carried out at 37 °C for 12–16 h with an enzyme to substrate ratio of 1:50 (catalogue no. V5111; Promega).

    Article Title: Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches
    Article Snippet: Proteins were precipitated with acetone overnight, and then dissolved in 40μl of 50mM NH4 HCO3 (v/v, pH = 8.5) containing 1μg of trypsine (Promega V51 11). .. Samples were incubated at 37°C overnight, and trypsinization was stopped with formic acid (0,1% final v/v).

    Article Title: Proteomic analysis reveals novel insights into tanshinones biosynthesis in Salvia miltiorrhiza hairy roots
    Article Snippet: .. Proteins were recovered by acetone precipitation and dissolved in 20 µL 25 mM (w/v) NH4 HCO3 (pH 8.5) containing 2 µg trypsin (Promega V51 11) and incubated at 37 °C overnight. ..

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: After that, spots were briefly washed with 100 mM NH4 HCO3 and again incubated for 15 min in 50% (v/v) acetonitrile (ACN), for 3 min in 100% ACN, for 3 min in 100 mM NH4 HCO3 , for 15 min in 50 mM NH4 HCO3 in 50% (v/v) ACN and finally dried under vacuum. .. The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited.

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: The reaction was cleared by centrifugation then incubated with protein A beads (20 μl/IP) for 1 hr. .. Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Mass Spectrometry:

    Article Title: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
    Article Snippet: Paragraph title: Mass spectrometry analysis ... After destaining and alkylation, each piece of gel was incubated with trypsin (Promega, http://worldwide.promega.com/search-results/?q=V5111 ) and extracted as described in.

    Article Title: A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene
    Article Snippet: Paragraph title: Mass spectrometry and data analysis ... Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI, USA) and digested peptides were extracted in 5% formic acid/50% acetonitrile and separated using C18 reversed phase LC column (75 micron × 15cm, Pepmap 300, 5 micron particle size) (Dionex, Sunnyvale, CA, USA).

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Article Title: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust
    Article Snippet: .. Protein in-gel digestion and mass spectrometry protein characterization Spots were excised from cCBB-stained 2-DE gels and in-gel digested with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] as previously described [ ]. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the Nano-ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA). .. A Q-TOF Ultima tandem mass spectrometer couple with a Nanoaquity HPLC system (Waters) with electrospray ionization was used to analyze the eluting peptides.

    BIA-KA:

    Article Title: Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes
    Article Snippet: Supernatant was collected and protein estimation was carried out by using bicinchoninic acid assay (BCA) . .. Trypsin digestion was carried out at 37 °C for 12–16 h with an enzyme to substrate ratio of 1:50 (catalogue no. V5111; Promega).

    Modification:

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: .. The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Article Title: Quantitative phosphoproteomic analysis of T-cell receptor signaling
    Article Snippet: .. Protein sample: 1 × 108 cells or 10 mg protein Cell lysis buffer: 9 M urea, 1 mM Na orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate in 20 mM HEPES, pH 8.0 ( see Note ) 45 mM dithiothreitol and 100 mM iodoacetimide Trypsin, sequencing grade, modified, TPCK treated, affinity purified (Cat, V5113, Promega, NJ, USA) .. Solvent A containing 0.1% of trifluroacetic acid (TFA) Solvent B containing 0.1% of TFA in 40% acetonitrile Sep-Pack light C18 cartridges (WAT020515, Waters, MA, USA) and extraction manifold apparatus (WAT200677, Waters) 10 mL disposable syringe

    Article Title: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust
    Article Snippet: .. Protein in-gel digestion and mass spectrometry protein characterization Spots were excised from cCBB-stained 2-DE gels and in-gel digested with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] as previously described [ ]. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the Nano-ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Western Blot:

    Article Title: Isolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from Capra hircus ear cartilage
    Article Snippet: Paragraph title: SDS-PAGE and western nlot analysis ... The dehydrated gel pieces were digested with sequence graded trypsin at 37 °C air bath for overnight (Product number V5111, Promega gold, Madison, WI) to cleave the protein at specific peptide bonds depending on the amino-acid sequences .

    High Performance Liquid Chromatography:

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Article Title: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust
    Article Snippet: Protein in-gel digestion and mass spectrometry protein characterization Spots were excised from cCBB-stained 2-DE gels and in-gel digested with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] as previously described [ ]. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the Nano-ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA). .. A Q-TOF Ultima tandem mass spectrometer couple with a Nanoaquity HPLC system (Waters) with electrospray ionization was used to analyze the eluting peptides.

    Flow Cytometry:

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited. .. Chromatography separations were obtained on a BioBasic C18 column (180 μm I.D × 150 mm length, 5 μm particle size), using a linear gradient from 5% to 80% solvent B [solvent A: 0.1% (v/v) formic acid; solvent B: ACN containing 0.1% (v/v) formic acid] with a flow of 2.5 μl/min.

    Article Title: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust
    Article Snippet: Protein in-gel digestion and mass spectrometry protein characterization Spots were excised from cCBB-stained 2-DE gels and in-gel digested with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] as previously described [ ]. .. Chromatography separations were carried out on a ZORBAX 300SB-C18 column (75 μm I.D × 150 mm length, 3.5 μm particle size, Agilent Tecnologies ®), using a linear gradient from 5 to 60% solvent B [solvent A: 0.1% (v/v) formic acid; solvent B: ACN containing 0.1% (v/v) formic acid] with a flow of 300 nl/min.

    Chromatography:

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited. .. Chromatography separations were obtained on a BioBasic C18 column (180 μm I.D × 150 mm length, 5 μm particle size), using a linear gradient from 5% to 80% solvent B [solvent A: 0.1% (v/v) formic acid; solvent B: ACN containing 0.1% (v/v) formic acid] with a flow of 2.5 μl/min.

    Article Title: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust
    Article Snippet: Protein in-gel digestion and mass spectrometry protein characterization Spots were excised from cCBB-stained 2-DE gels and in-gel digested with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] as previously described [ ]. .. Chromatography separations were carried out on a ZORBAX 300SB-C18 column (75 μm I.D × 150 mm length, 3.5 μm particle size, Agilent Tecnologies ®), using a linear gradient from 5 to 60% solvent B [solvent A: 0.1% (v/v) formic acid; solvent B: ACN containing 0.1% (v/v) formic acid] with a flow of 300 nl/min.

    Concentration Assay:

    Article Title: Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches
    Article Snippet: Proteins were precipitated with acetone overnight, and then dissolved in 40μl of 50mM NH4 HCO3 (v/v, pH = 8.5) containing 1μg of trypsine (Promega V51 11). .. Trypsic peptides (0.8μg/μL final concentration, v/v) were separated on reverse phase column (length: 25cm, diameter: 75μm, particles: 3μm, outlet: 300nL/min, PepMap C18, Dionex), and submitted to an ACN gradient (4 to 35%, v/v) for 120 minutes.

    Transferring:

    Article Title: Proteomic characterization of serine hydrolase activity and composition in normal urine
    Article Snippet: For in-gel digestion, tagged proteins were separated on LDS-PAGE, bands excised from gels and analyzed by MS-MS. For in-solution digestion, the Dynabeads® were washed (50 mM PBS, 0.25% Tween 20), and tagged proteins digested with 250 ng trypsin [Promega V5111]. .. Peptide digests were desalted and purified off-line using ZipTip® C18 Pipette Tips [Millipore], were frozen at -80°C and dried using a speed vacuum.

    SDS Page:

    Article Title: Isolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from Capra hircus ear cartilage
    Article Snippet: Paragraph title: SDS-PAGE and western nlot analysis ... The dehydrated gel pieces were digested with sequence graded trypsin at 37 °C air bath for overnight (Product number V5111, Promega gold, Madison, WI) to cleave the protein at specific peptide bonds depending on the amino-acid sequences .

    Article Title: Proteomic analysis of human osteoarthritis synovial fluid
    Article Snippet: Paragraph title: SDS-PAGE and in-gel digestion ... Trypsin digestion was carried out at 37˚C for 12–16 hrs (Catalog number: V5111 Sequencing grade, Promega, Madison, WI, US).

    Article Title: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
    Article Snippet: Mass spectrometry analysis Samples from co-immunoprecipitation experiments were separated by 15% SDS-PAGE gel electrophoresis. .. After destaining and alkylation, each piece of gel was incubated with trypsin (Promega, http://worldwide.promega.com/search-results/?q=V5111 ) and extracted as described in.

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: CUL3 immunocomplexes were resolved by SDS–PAGE, stained with Coomassie blue, and the protein bands in the CUL3 IP and corresponding gel bands in pre-immune IP were excised and subjected to LC/MS at the University of Nebraska Proteomics Core Facility (Lincoln, NE) as described previously ( ). .. Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Tandem Mass Spectroscopy:

    Article Title: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
    Article Snippet: After destaining and alkylation, each piece of gel was incubated with trypsin (Promega, http://worldwide.promega.com/search-results/?q=V5111 ) and extracted as described in. .. MS and HCD MS/MS data were acquired under high resolution mode (60000 and 15000, respectively).

    Article Title: A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene
    Article Snippet: Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI, USA) and digested peptides were extracted in 5% formic acid/50% acetonitrile and separated using C18 reversed phase LC column (75 micron × 15cm, Pepmap 300, 5 micron particle size) (Dionex, Sunnyvale, CA, USA). .. The system was user-controlled employing MassLynx software (v 4.1, Waters) in data-dependant acquisition mode with the following parameters: 0.9-sec survey scan (380–1900 Da) followed by up to three 1.4-s MS/MS acquisitions (60–1900 Da).

    Article Title: Proteomic characterization of serine hydrolase activity and composition in normal urine
    Article Snippet: .. For in-gel digestion, tagged proteins were separated on LDS-PAGE, bands excised from gels and analyzed by MS-MS. For in-solution digestion, the Dynabeads® were washed (50 mM PBS, 0.25% Tween 20), and tagged proteins digested with 250 ng trypsin [Promega V5111]. .. Peptide digests were desalted and purified off-line using ZipTip® C18 Pipette Tips [Millipore], were frozen at -80°C and dried using a speed vacuum.

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA). .. The system was user controlled employing MassLynx software (v 4.1, Waters) in data-dependant acquisition mode with the following parameters: 0.9-sec survey scan (380–1900 Da) followed by up to three 1.4-sec MS/MS acquisitions (60–1900 Da).

    Sequencing:

    Article Title: The inhibition, reactivation and mechanism of VX-, sarin-, fluoro-VX and fluoro-sarin surrogates following their interaction with HuAChE and HuBuChE.
    Article Snippet: .. Trypsin was sequencing grade V5111 and purchased from Promega (Madison, WI). .. C4 ZipTips were obtained from Millipore (Billerica, MA).

    Article Title: Isolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from Capra hircus ear cartilage
    Article Snippet: .. The dehydrated gel pieces were digested with sequence graded trypsin at 37 °C air bath for overnight (Product number V5111, Promega gold, Madison, WI) to cleave the protein at specific peptide bonds depending on the amino-acid sequences . .. Trypsin powder (100 µg/vial) was resuspended according to the manufacture’s protocol (Promega gold, Madison, WI).

    Article Title: Proteomic analysis of human osteoarthritis synovial fluid
    Article Snippet: .. Trypsin digestion was carried out at 37˚C for 12–16 hrs (Catalog number: V5111 Sequencing grade, Promega, Madison, WI, US). ..

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: .. The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Article Title: Quantitative phosphoproteomic analysis of T-cell receptor signaling
    Article Snippet: .. Protein sample: 1 × 108 cells or 10 mg protein Cell lysis buffer: 9 M urea, 1 mM Na orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate in 20 mM HEPES, pH 8.0 ( see Note ) 45 mM dithiothreitol and 100 mM iodoacetimide Trypsin, sequencing grade, modified, TPCK treated, affinity purified (Cat, V5113, Promega, NJ, USA) .. Solvent A containing 0.1% of trifluroacetic acid (TFA) Solvent B containing 0.1% of TFA in 40% acetonitrile Sep-Pack light C18 cartridges (WAT020515, Waters, MA, USA) and extraction manifold apparatus (WAT200677, Waters) 10 mL disposable syringe

    Article Title: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust
    Article Snippet: .. Protein in-gel digestion and mass spectrometry protein characterization Spots were excised from cCBB-stained 2-DE gels and in-gel digested with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] as previously described [ ]. .. The LC-ESI-MS/MS experiments were conducted using a Surveyor (MS pump Plus) HPLC system directly connected to the Nano-ESI source of a Finnigan LCQ DECA XP MAX ion trap mass spectrometer (ThermoFisher Scientific Inc., Waltham, USA).

    Injection:

    Article Title: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
    Article Snippet: After destaining and alkylation, each piece of gel was incubated with trypsin (Promega, http://worldwide.promega.com/search-results/?q=V5111 ) and extracted as described in. .. Resulting peptides were injected to LC-MS/MS system (RSLCnano connected on line to Orbitrap Elite; Thermo Fisher Scientific).

    Recombinant:

    Article Title: The inhibition, reactivation and mechanism of VX-, sarin-, fluoro-VX and fluoro-sarin surrogates following their interaction with HuAChE and HuBuChE.
    Article Snippet: Trypsin was sequencing grade V5111 and purchased from Promega (Madison, WI). .. Recombinant human acetylcholinesterase (HuAChE) from Sigma-Aldrich (C1682; St Lois, MO) and recombinant human serum butyrylcholinesterase (HuBChE) from Lee Biosolutions (Maryland Heights, MO).

    Nucleic Acid Electrophoresis:

    Article Title: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
    Article Snippet: Mass spectrometry analysis Samples from co-immunoprecipitation experiments were separated by 15% SDS-PAGE gel electrophoresis. .. After destaining and alkylation, each piece of gel was incubated with trypsin (Promega, http://worldwide.promega.com/search-results/?q=V5111 ) and extracted as described in.

    Labeling:

    Article Title: Proteomic characterization of serine hydrolase activity and composition in normal urine
    Article Snippet: Labeled enzymes were co-immunoprecipitated overnight using 10 μl anti-TAMRA antibody [A6397, Invitrogen] bound to 40 μl of Dynabeads® [Dynabeads® protein G, Invitrogen], and then separated with a magnet. .. For in-gel digestion, tagged proteins were separated on LDS-PAGE, bands excised from gels and analyzed by MS-MS. For in-solution digestion, the Dynabeads® were washed (50 mM PBS, 0.25% Tween 20), and tagged proteins digested with 250 ng trypsin [Promega V5111].

    Purification:

    Article Title: Proteomic characterization of serine hydrolase activity and composition in normal urine
    Article Snippet: For in-gel digestion, tagged proteins were separated on LDS-PAGE, bands excised from gels and analyzed by MS-MS. For in-solution digestion, the Dynabeads® were washed (50 mM PBS, 0.25% Tween 20), and tagged proteins digested with 250 ng trypsin [Promega V5111]. .. Peptide digests were desalted and purified off-line using ZipTip® C18 Pipette Tips [Millipore], were frozen at -80°C and dried using a speed vacuum.

    Lysis:

    Article Title: Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches
    Article Snippet: Briefly, proteins were extracted in 6M guanidinium chloride pH = 8.5 (Lysis buffer of ICPL kit (SERVA, Germany)) by ultrasonication (3x10 seconds, 20% amplitude, U50 IKAtechnik). .. Proteins were precipitated with acetone overnight, and then dissolved in 40μl of 50mM NH4 HCO3 (v/v, pH = 8.5) containing 1μg of trypsine (Promega V51 11).

    Article Title: Quantitative phosphoproteomic analysis of T-cell receptor signaling
    Article Snippet: .. Protein sample: 1 × 108 cells or 10 mg protein Cell lysis buffer: 9 M urea, 1 mM Na orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate in 20 mM HEPES, pH 8.0 ( see Note ) 45 mM dithiothreitol and 100 mM iodoacetimide Trypsin, sequencing grade, modified, TPCK treated, affinity purified (Cat, V5113, Promega, NJ, USA) .. Solvent A containing 0.1% of trifluroacetic acid (TFA) Solvent B containing 0.1% of TFA in 40% acetonitrile Sep-Pack light C18 cartridges (WAT020515, Waters, MA, USA) and extraction manifold apparatus (WAT200677, Waters) 10 mL disposable syringe

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: The cleared extract was diluted with 0.1% NP-40 lysis buffer (1:2), then immunoprecipitated by anti-Xenopus CUL3 antibody (20 μg) or pre-immune serum overnight. .. Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Chromatin Immunoprecipitation:

    Article Title: Isolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from Capra hircus ear cartilage
    Article Snippet: The dehydrated gel pieces were digested with sequence graded trypsin at 37 °C air bath for overnight (Product number V5111, Promega gold, Madison, WI) to cleave the protein at specific peptide bonds depending on the amino-acid sequences . .. Lyophilized peptides were mixed with equal vol. of matrix solution of α-cyano-4-hydroxycinnamic acid (Bruker Daltonics, Germany) in 30% acetonitrile and 70% TFA (0.1% TFA in MQ water) and spotted on to the Anchor chip target plate (600/384F, Bruker Daltonics).

    Affinity Purification:

    Article Title: Quantitative phosphoproteomic analysis of T-cell receptor signaling
    Article Snippet: .. Protein sample: 1 × 108 cells or 10 mg protein Cell lysis buffer: 9 M urea, 1 mM Na orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate in 20 mM HEPES, pH 8.0 ( see Note ) 45 mM dithiothreitol and 100 mM iodoacetimide Trypsin, sequencing grade, modified, TPCK treated, affinity purified (Cat, V5113, Promega, NJ, USA) .. Solvent A containing 0.1% of trifluroacetic acid (TFA) Solvent B containing 0.1% of TFA in 40% acetonitrile Sep-Pack light C18 cartridges (WAT020515, Waters, MA, USA) and extraction manifold apparatus (WAT200677, Waters) 10 mL disposable syringe

    Article Title: Proteomic characterization of serine hydrolase activity and composition in normal urine
    Article Snippet: Paragraph title: Affinity purification & identification of active serine hydrolases ... For in-gel digestion, tagged proteins were separated on LDS-PAGE, bands excised from gels and analyzed by MS-MS. For in-solution digestion, the Dynabeads® were washed (50 mM PBS, 0.25% Tween 20), and tagged proteins digested with 250 ng trypsin [Promega V5111].

    Immu-Puri:

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: Paragraph title: Xenopus egg extract preparation, immunopurification and mass spectrometric analysis ... Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Software:

    Article Title: The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex
    Article Snippet: After destaining and alkylation, each piece of gel was incubated with trypsin (Promega, http://worldwide.promega.com/search-results/?q=V5111 ) and extracted as described in. .. The mass spectrometric data were processed by the Proteome Discoverer software (version 1.4, Thermo Fisher Scientific) with in-house Mascot (version 2.4.1, Matrix Science).

    Article Title: A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene
    Article Snippet: Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI, USA) and digested peptides were extracted in 5% formic acid/50% acetonitrile and separated using C18 reversed phase LC column (75 micron × 15cm, Pepmap 300, 5 micron particle size) (Dionex, Sunnyvale, CA, USA). .. The system was user-controlled employing MassLynx software (v 4.1, Waters) in data-dependant acquisition mode with the following parameters: 0.9-sec survey scan (380–1900 Da) followed by up to three 1.4-s MS/MS acquisitions (60–1900 Da).

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA). .. The system was user controlled employing MassLynx software (v 4.1, Waters) in data-dependant acquisition mode with the following parameters: 0.9-sec survey scan (380–1900 Da) followed by up to three 1.4-sec MS/MS acquisitions (60–1900 Da).

    Sample Prep:

    Article Title: Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes
    Article Snippet: Paragraph title: Sample preparation, in-solution digestion and TMT labelling ... Trypsin digestion was carried out at 37 °C for 12–16 h with an enzyme to substrate ratio of 1:50 (catalogue no. V5111; Promega).

    Acid Assay:

    Article Title: Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes
    Article Snippet: Supernatant was collected and protein estimation was carried out by using bicinchoninic acid assay (BCA) . .. Trypsin digestion was carried out at 37 °C for 12–16 h with an enzyme to substrate ratio of 1:50 (catalogue no. V5111; Promega).

    Immunoprecipitation:

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: The cleared extract was diluted with 0.1% NP-40 lysis buffer (1:2), then immunoprecipitated by anti-Xenopus CUL3 antibody (20 μg) or pre-immune serum overnight. .. Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: CUL3 immunocomplexes were resolved by SDS–PAGE, stained with Coomassie blue, and the protein bands in the CUL3 IP and corresponding gel bands in pre-immune IP were excised and subjected to LC/MS at the University of Nebraska Proteomics Core Facility (Lincoln, NE) as described previously ( ). .. Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Staining:

    Article Title: Proteomic analysis of human osteoarthritis synovial fluid
    Article Snippet: The gel was then stained using colloidal Coomassie blue. .. Trypsin digestion was carried out at 37˚C for 12–16 hrs (Catalog number: V5111 Sequencing grade, Promega, Madison, WI, US).

    Article Title: Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis
    Article Snippet: Protein in-gel digestion and LC-ESI-MS/MS analysis Spots excised from gels stained with cCBB were digested as described by Magni and co-workers [ ] with some refinements. .. The following phases consisting in the protein digestion with trypsin [Sequencing grade modified Trypsin V5111, Promega, Madison] and in the recovery of peptides were carried out as described in the article above cited.

    Article Title: The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A
    Article Snippet: CUL3 immunocomplexes were resolved by SDS–PAGE, stained with Coomassie blue, and the protein bands in the CUL3 IP and corresponding gel bands in pre-immune IP were excised and subjected to LC/MS at the University of Nebraska Proteomics Core Facility (Lincoln, NE) as described previously ( ). .. Briefly, gel pieces were digested by trypsin (no.V5111, Promega, Madison, WI) and digested peptides were extracted in 5% formic acid / 50% acetonitrile and separated using C18 reversed phase LC column (75 micron×15 cm, BEH 130, 1.7 micron Waters, Milford, MA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Promega trypsin
    Trypsin, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin/product/Promega
    Average 90 stars, based on 1408 article reviews
    Price from $9.99 to $1999.99
    trypsin - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results