sequencing grade modified trypsin  (Millipore)


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    Structured Review

    Millipore sequencing grade modified trypsin
    Sequencing Grade Modified Trypsin, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing grade modified trypsin/product/Millipore
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    sequencing grade modified trypsin - by Bioz Stars, 2020-02
    94/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
    Article Snippet: .. Baker, cat. no. 6903) Formic acid (FA) (EMD, cat. no. 11670) Trifluoroacetic acid HPLC grade (TFA) (EMD, cat. no. TX1276) Methanol HPLC grade (Burdick & Jackson, cat. no. AH230-4) Acetonitrile HPLC grade (ACN) (Burdick & Jackson, cat. no. AH015-4) Water HPLC grade (Burdick & Jackson, cat. no. AH365-4) Sequencing grade modified trypsin (Promega, cat. no. V5111 or V5113) Tris(hydroxymethyl)aminomethane (Tris) (Sigma, cat. no. T6066) Sodium chloride (Sigma, cat. no. S6191) Calcium chloride (Sigma, cat. no. C5670) Urea (Sigma, cat. no. U6504) Complete protease inhibitors cocktail tablets mini, EDTA-free (Roche, cat. no. 11 846 170 001) Phenylmethylsulfonyl fluoride (PMSF) (Sigma, cat. no. P7626) Sodium orthovanadate (Sigma, cat. no. S6508) Sodium fluoride (Sigma, cat. no. S7920) Sodium β-glycerophosphate (Sigma, cat. no. G6251) Sodium pyrophosphate (Sigma, cat. no. S6422) Bicinchoninic acid (BCA) protein assay kit (Pierce, cat. no. 23225) Dithiothreitol (Biovectra, cat. no. 1369) Iodoacetamide (Sigma, cat. no. I1149) Glass beads 0.5-mm diameter (Biospec, cat. no. 11079105) PhosSelect iron affinity gel (IMAC beads) (Sigma, cat. no. P9740) stored in aliquots at −20 °C Potassium phosphate monobasic (Sigma, cat. no. P5379) Potassium phosphate dibasic (Sigma, cat. no. P9666) Phosphoric acid (J.T. .. Baker, cat. no. 7664-38-2) Potassium chloride (Sigma, cat. no. P4504)

    Flow Cytometry:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: Per SEC run, 1 mg native proteome (by OD280) was injected and fractionated at 500 μl/min flow rate at 4°C, collecting fractions at 0.19 min per fraction from 10 to 28 min post‐injection, fractions 3–83 of which were considered relevant proteome elution range and considered for further analysis with fractionation index 1–81. .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Silver Staining:

    Article Title: Proteome-wide enrichment of proteins modified by lysine methylation
    Article Snippet: Nonidet P-40 (NP-40; American Bioanalytical, cat. no. AB01425) cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche, cat. no. 04693132001) Protease inhibitor cocktail for use with fungal and yeast extracts (Sigma, cat. no. P8215) PMSF (Sigma, cat. no. 93482) Lysozyme (Affymetrix, cat. no. 18645) Glutathione (GSH) Sepharose 4B (GE Healthcare, cat. no. 17-0756) Acid-washed glass beads, 425–600 mm (Sigma, cat. no G8772) Laemmli sample buffer, 4× (Bio-Rad, cat. no. 161-0747) SDS-PAGE Tris/Glycine/SDS running buffer (Bio-Rad, cat. no. 161-0732) L-glutathione, reduced (Sigma, cat. no. G4251) SilverQuest silver staining kit (Invitrogen, cat. no. LC6070) Ethanol, 95% (vol/vol) (Gold Shield, cat. no. 412602) Glacial acetic acid (Fisher, cat. no. A490) ! .. Sequencing-grade modified trypsin (Promega, cat. no. V5113) Urea (Sigma, cat. no. U5378) Liquid nitrogen !

    Sample Prep:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: An aliquot of the unfractionated mild proteome extract was included in peptide sample preparation and LC‐MS analysis. .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Mass Spectrometry:

    Article Title: HCN Regulates Cellular Processes through Posttranslational Modification of Proteins by S-cyanylation
    Article Snippet: To identify S -cyanylated proteins, tryptic digestion was performed in solution of root extracts, and peptide analysis was carried out using a nano liquid chromatography system coupled to a high-speed mass spectrometer at the Proteomics Facility of the Centro Nacional de Biotecnología, Spain. .. An aliquot of 40 μg of protein from each treatment group was digested with sequencing-grade modified trypsin (Sigma-Aldrich) at 37°C overnight on a shaker.

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: Paragraph title: Preparation of native HEK293 proteome and fractions for MS analysis ... Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Protease Inhibitor:

    Article Title: Proteome-wide enrichment of proteins modified by lysine methylation
    Article Snippet: Nonidet P-40 (NP-40; American Bioanalytical, cat. no. AB01425) cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche, cat. no. 04693132001) Protease inhibitor cocktail for use with fungal and yeast extracts (Sigma, cat. no. P8215) PMSF (Sigma, cat. no. 93482) Lysozyme (Affymetrix, cat. no. 18645) Glutathione (GSH) Sepharose 4B (GE Healthcare, cat. no. 17-0756) Acid-washed glass beads, 425–600 mm (Sigma, cat. no G8772) Laemmli sample buffer, 4× (Bio-Rad, cat. no. 161-0747) SDS-PAGE Tris/Glycine/SDS running buffer (Bio-Rad, cat. no. 161-0732) L-glutathione, reduced (Sigma, cat. no. G4251) SilverQuest silver staining kit (Invitrogen, cat. no. LC6070) Ethanol, 95% (vol/vol) (Gold Shield, cat. no. 412602) Glacial acetic acid (Fisher, cat. no. A490) ! .. Sequencing-grade modified trypsin (Promega, cat. no. V5113) Urea (Sigma, cat. no. U5378) Liquid nitrogen !

    Centrifugation:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: After 5 min of centrifugation at 16.9 × g , 4°C, the supernatant was directly subjected to fractionation on a Yarra‐SEC‐4000 column (300 × 7.8 mm, pore size 500 Å, particle size 3 μm, Phenomenex, CA, USA). .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Size-exclusion Chromatography:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: The fractions collected from two consecutive SEC fractionations of the same extract (2 × 1 mg) were pooled for subsequent bottom‐up proteomic analysis. .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Sequencing:

    Article Title: Proteome-wide enrichment of proteins modified by lysine methylation
    Article Snippet: .. Sequencing-grade modified trypsin (Promega, cat. no. V5113) Urea (Sigma, cat. no. U5378) Liquid nitrogen ! ..

    Article Title: Labeling and Identification of Direct Kinase Substrates
    Article Snippet: .. Methanol Chloroform Trypsin, sequencing grade (#V5113, Promega, Madison, WI) Ammonium acetate Urea (#U5378, Sigma-Aldrich) Sep-Pak cartridges (WAT020515, Waters, Milford, MA) Glacial acetic acid Acetonitrile (ACN) .. Sulfolink beads (#20401, Thermo/Pierce) Formic acid Dithiothreitol (DTT) Note: DTT is used for its nucleophilic thiol groups; other reducing agents may not be substituted.

    Article Title: HCN Regulates Cellular Processes through Posttranslational Modification of Proteins by S-cyanylation
    Article Snippet: .. An aliquot of 40 μg of protein from each treatment group was digested with sequencing-grade modified trypsin (Sigma-Aldrich) at 37°C overnight on a shaker. ..

    Article Title: The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
    Article Snippet: .. Baker, cat. no. 6903) Formic acid (FA) (EMD, cat. no. 11670) Trifluoroacetic acid HPLC grade (TFA) (EMD, cat. no. TX1276) Methanol HPLC grade (Burdick & Jackson, cat. no. AH230-4) Acetonitrile HPLC grade (ACN) (Burdick & Jackson, cat. no. AH015-4) Water HPLC grade (Burdick & Jackson, cat. no. AH365-4) Sequencing grade modified trypsin (Promega, cat. no. V5111 or V5113) Tris(hydroxymethyl)aminomethane (Tris) (Sigma, cat. no. T6066) Sodium chloride (Sigma, cat. no. S6191) Calcium chloride (Sigma, cat. no. C5670) Urea (Sigma, cat. no. U6504) Complete protease inhibitors cocktail tablets mini, EDTA-free (Roche, cat. no. 11 846 170 001) Phenylmethylsulfonyl fluoride (PMSF) (Sigma, cat. no. P7626) Sodium orthovanadate (Sigma, cat. no. S6508) Sodium fluoride (Sigma, cat. no. S7920) Sodium β-glycerophosphate (Sigma, cat. no. G6251) Sodium pyrophosphate (Sigma, cat. no. S6422) Bicinchoninic acid (BCA) protein assay kit (Pierce, cat. no. 23225) Dithiothreitol (Biovectra, cat. no. 1369) Iodoacetamide (Sigma, cat. no. I1149) Glass beads 0.5-mm diameter (Biospec, cat. no. 11079105) PhosSelect iron affinity gel (IMAC beads) (Sigma, cat. no. P9740) stored in aliquots at −20 °C Potassium phosphate monobasic (Sigma, cat. no. P5379) Potassium phosphate dibasic (Sigma, cat. no. P9666) Phosphoric acid (J.T. .. Baker, cat. no. 7664-38-2) Potassium chloride (Sigma, cat. no. P4504)

    Fractionation:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: Per SEC run, 1 mg native proteome (by OD280) was injected and fractionated at 500 μl/min flow rate at 4°C, collecting fractions at 0.19 min per fraction from 10 to 28 min post‐injection, fractions 3–83 of which were considered relevant proteome elution range and considered for further analysis with fractionation index 1–81. .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Molecular Weight:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: Apparent molecular weight per fraction was log‐linearly calibrated based on column performance check protein mix analyzed prior and after each experimental replicate (AL0‐3042, Phenomenex, CA, USA). .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: An aliquot of the unfractionated mild proteome extract was included in peptide sample preparation and LC‐MS analysis. .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    BIA-KA:

    Article Title: The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
    Article Snippet: .. Baker, cat. no. 6903) Formic acid (FA) (EMD, cat. no. 11670) Trifluoroacetic acid HPLC grade (TFA) (EMD, cat. no. TX1276) Methanol HPLC grade (Burdick & Jackson, cat. no. AH230-4) Acetonitrile HPLC grade (ACN) (Burdick & Jackson, cat. no. AH015-4) Water HPLC grade (Burdick & Jackson, cat. no. AH365-4) Sequencing grade modified trypsin (Promega, cat. no. V5111 or V5113) Tris(hydroxymethyl)aminomethane (Tris) (Sigma, cat. no. T6066) Sodium chloride (Sigma, cat. no. S6191) Calcium chloride (Sigma, cat. no. C5670) Urea (Sigma, cat. no. U6504) Complete protease inhibitors cocktail tablets mini, EDTA-free (Roche, cat. no. 11 846 170 001) Phenylmethylsulfonyl fluoride (PMSF) (Sigma, cat. no. P7626) Sodium orthovanadate (Sigma, cat. no. S6508) Sodium fluoride (Sigma, cat. no. S7920) Sodium β-glycerophosphate (Sigma, cat. no. G6251) Sodium pyrophosphate (Sigma, cat. no. S6422) Bicinchoninic acid (BCA) protein assay kit (Pierce, cat. no. 23225) Dithiothreitol (Biovectra, cat. no. 1369) Iodoacetamide (Sigma, cat. no. I1149) Glass beads 0.5-mm diameter (Biospec, cat. no. 11079105) PhosSelect iron affinity gel (IMAC beads) (Sigma, cat. no. P9740) stored in aliquots at −20 °C Potassium phosphate monobasic (Sigma, cat. no. P5379) Potassium phosphate dibasic (Sigma, cat. no. P9666) Phosphoric acid (J.T. .. Baker, cat. no. 7664-38-2) Potassium chloride (Sigma, cat. no. P4504)

    Modification:

    Article Title: Proteome-wide enrichment of proteins modified by lysine methylation
    Article Snippet: .. Sequencing-grade modified trypsin (Promega, cat. no. V5113) Urea (Sigma, cat. no. U5378) Liquid nitrogen ! ..

    Article Title: HCN Regulates Cellular Processes through Posttranslational Modification of Proteins by S-cyanylation
    Article Snippet: .. An aliquot of 40 μg of protein from each treatment group was digested with sequencing-grade modified trypsin (Sigma-Aldrich) at 37°C overnight on a shaker. ..

    Article Title: The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
    Article Snippet: .. Baker, cat. no. 6903) Formic acid (FA) (EMD, cat. no. 11670) Trifluoroacetic acid HPLC grade (TFA) (EMD, cat. no. TX1276) Methanol HPLC grade (Burdick & Jackson, cat. no. AH230-4) Acetonitrile HPLC grade (ACN) (Burdick & Jackson, cat. no. AH015-4) Water HPLC grade (Burdick & Jackson, cat. no. AH365-4) Sequencing grade modified trypsin (Promega, cat. no. V5111 or V5113) Tris(hydroxymethyl)aminomethane (Tris) (Sigma, cat. no. T6066) Sodium chloride (Sigma, cat. no. S6191) Calcium chloride (Sigma, cat. no. C5670) Urea (Sigma, cat. no. U6504) Complete protease inhibitors cocktail tablets mini, EDTA-free (Roche, cat. no. 11 846 170 001) Phenylmethylsulfonyl fluoride (PMSF) (Sigma, cat. no. P7626) Sodium orthovanadate (Sigma, cat. no. S6508) Sodium fluoride (Sigma, cat. no. S7920) Sodium β-glycerophosphate (Sigma, cat. no. G6251) Sodium pyrophosphate (Sigma, cat. no. S6422) Bicinchoninic acid (BCA) protein assay kit (Pierce, cat. no. 23225) Dithiothreitol (Biovectra, cat. no. 1369) Iodoacetamide (Sigma, cat. no. I1149) Glass beads 0.5-mm diameter (Biospec, cat. no. 11079105) PhosSelect iron affinity gel (IMAC beads) (Sigma, cat. no. P9740) stored in aliquots at −20 °C Potassium phosphate monobasic (Sigma, cat. no. P5379) Potassium phosphate dibasic (Sigma, cat. no. P9666) Phosphoric acid (J.T. .. Baker, cat. no. 7664-38-2) Potassium chloride (Sigma, cat. no. P4504)

    Injection:

    Article Title: Complex‐centric proteome profiling by SEC‐ SWATH‐ MS
    Article Snippet: Per SEC run, 1 mg native proteome (by OD280) was injected and fractionated at 500 μl/min flow rate at 4°C, collecting fractions at 0.19 min per fraction from 10 to 28 min post‐injection, fractions 3–83 of which were considered relevant proteome elution range and considered for further analysis with fractionation index 1–81. .. Proteins were proteolyzed to peptide level by trypsin digestion (Promega V5111) in the presence of 1% sodium deoxycholate (Sigma‐Aldrich D6750), reduced, alkylated, and de‐salted on C18 reversed phase (96‐Well MACROSpin Plate, The Nest Group, MA, USA), and each sample was supplemented with equal amounts of internal retention time calibration peptides (iRT kit, Biognosys, CH).

    Purification:

    Article Title: Proteome-wide enrichment of proteins modified by lysine methylation
    Article Snippet: Deionized water (Milli-Q purified, Millipore) Ammonium bicarbonate (Sigma, cat. no. A6141) DTT (Bio-Rad, cat. no. 161-0611) Iodoacetamide (Sigma, cat. no. I1149) Acetonitrile (EMD Millipore, cat. no. AX0151-1) Formic acid (Sigma, cat. no. 94318) ! .. Sequencing-grade modified trypsin (Promega, cat. no. V5113) Urea (Sigma, cat. no. U5378) Liquid nitrogen !

    Liquid Chromatography:

    Article Title: HCN Regulates Cellular Processes through Posttranslational Modification of Proteins by S-cyanylation
    Article Snippet: To identify S -cyanylated proteins, tryptic digestion was performed in solution of root extracts, and peptide analysis was carried out using a nano liquid chromatography system coupled to a high-speed mass spectrometer at the Proteomics Facility of the Centro Nacional de Biotecnología, Spain. .. An aliquot of 40 μg of protein from each treatment group was digested with sequencing-grade modified trypsin (Sigma-Aldrich) at 37°C overnight on a shaker.

    SDS Page:

    Article Title: Proteome-wide enrichment of proteins modified by lysine methylation
    Article Snippet: Nonidet P-40 (NP-40; American Bioanalytical, cat. no. AB01425) cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche, cat. no. 04693132001) Protease inhibitor cocktail for use with fungal and yeast extracts (Sigma, cat. no. P8215) PMSF (Sigma, cat. no. 93482) Lysozyme (Affymetrix, cat. no. 18645) Glutathione (GSH) Sepharose 4B (GE Healthcare, cat. no. 17-0756) Acid-washed glass beads, 425–600 mm (Sigma, cat. no G8772) Laemmli sample buffer, 4× (Bio-Rad, cat. no. 161-0747) SDS-PAGE Tris/Glycine/SDS running buffer (Bio-Rad, cat. no. 161-0732) L-glutathione, reduced (Sigma, cat. no. G4251) SilverQuest silver staining kit (Invitrogen, cat. no. LC6070) Ethanol, 95% (vol/vol) (Gold Shield, cat. no. 412602) Glacial acetic acid (Fisher, cat. no. A490) ! .. Sequencing-grade modified trypsin (Promega, cat. no. V5113) Urea (Sigma, cat. no. U5378) Liquid nitrogen !

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  • 88
    Millipore sequencing grade modified porcine trypsin
    Sequencing Grade Modified Porcine Trypsin, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing grade modified porcine trypsin/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequencing grade modified porcine trypsin - by Bioz Stars, 2020-02
    88/100 stars
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