sequencing grade modified trypsin  (Millipore)


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    Name:
    Trypsin
    Description:

    Catalog Number:
    59429c
    Price:
    None
    Applications:
    Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.
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    Millipore sequencing grade modified trypsin

    https://www.bioz.com/result/sequencing grade modified trypsin/product/Millipore
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    sequencing grade modified trypsin - by Bioz Stars, 2020-09
    99/100 stars

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    Incubation:

    Article Title: Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors
    Article Snippet: .. After incubation at 35 °C for 1 h, the viral inoculates were replaced with serum-free medium containing L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (0.3 μg/mL) (Sigma-Aldrich) appropriate for each cell line. .. Cell culture supernatants were harvested at 12, 24, 48, and 72 h post infection (hpi) for virus titration in MDCK cells, as described above.

    Article Title: Draft genome of the honey bee ectoparasitic mite, Tropilaelaps mercedesae, is shaped by the parasitic life history
    Article Snippet: .. Proteomic-grade trypsin (Sigma) was added at a protein:trypsin ratio of 50:1 and incubated at 37°C overnight. .. To carry out the relative quantification, we compared the relative expression levels of the target mRNA to Ef-1α mRNA as the internal reference using the 2−ΔΔCt method.

    Article Title: Chlamydial and human heat shock protein 60s activate human vascular endothelium, smooth muscle cells, and macrophages
    Article Snippet: .. Human SMCs and ECs were first harvested by incubation with HBSS containing trypsin-EDTA (Sigma Chemical Co.). .. Cells were then incubated (30 min at 4°C) with the specific primary antibody: antibodies against human E-selectin (H18/7) , ICAM-1 (Hu5/3) , and VCAM-1(E1/6) ( ) were a generous gift of M. Gimbrone (Brigham and Women's Hospital, Boston, Massachusetts, USA); control mouse IgGs were purchased from PharMingen (San Diego, California, USA).

    Transfection:

    Article Title: Comparison of the virulence and transmissibility of canine H3N2 influenza viruses and characterization of their canine adaptation factors
    Article Snippet: .. After 30 h, 1 mL of Opti-MEM I containing 0.2 µg/mL of L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich) was added to the transfected cells. .. The supernatant was harvested after 48 h and injected into 11-day-old embryonated chicken eggs to propagate the virus.

    Article Title: Influenza HA Subtypes Demonstrate Divergent Phenotypes for Cleavage Activation and pH of Fusion: Implications for Host Range and Adaptation
    Article Snippet: .. At 16 hrs post transfection, HA-expressing Vero cells were washed once with PBS and treated with 5 µg/ml TPCK-Trypsin (Sigma), where indicated, at 37°C for 30 min. Trypsin-treated/untreated HA-expressing cells were subsequently overlaid with BSR-T7/5 target cells, which constitutively express the bacteriophage T7 RNA polymerase, and BSR-T7/5 cells were allowed to settle and adhere for 1 hr at 37°C. .. Following overlay with BSR-T7/5 target cells, cells were washed once with PBS and exposed to 1.0 ml PBS that had been pH adjusted using 100 mM citric acid to the indicated pH and incubated at 37°C for 5 min.

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  • 84
    Millipore trypsin sequencing grade modified
    Trypsin Sequencing Grade Modified, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin sequencing grade modified/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin sequencing grade modified - by Bioz Stars, 2020-09
    84/100 stars
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