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Merck & Co sequant zic philic column
Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequant zic philic column/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sequant zic philic column - by Bioz Stars, 2021-07
86/100 stars

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1) Product Images from "Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG"

Article Title: Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG

Journal: BMC Microbiology

doi: 10.1186/s12866-020-02006-z

Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )
Figure Legend Snippet: Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )

Techniques Used: Incubation

Related Articles

Injection:

Article Title: Host-dependent induction of disease tolerance to infection by tetracycline antibiotics
Article Snippet: Compound annotation was conducted by searching the mzCloud database with a mass accuracy of 3 ppm for precursor masses and 10 ppm for fragment ion masses as well as ChemSpider with a mass accuracy of 3 ppm using BioCyc, Human Metabolome Database, KEGG, MassBank and MetaboLights as databases. .. Targeted metabolomics Each sample was injected onto a SeQuant ZIC-pHILIC HPLC column (Merck, 100 x 2.1 mm; 5 µm) operated with an Ultimate 3000 HPLC system (Dionex, Thermo Fisher Scientific) at a flow rate of 100 µl/min and directly coupled to a TSQ Quantiva mass spectrometer (Thermo Fisher Scientific). ..

Article Title: Tetracycline Antibiotics Induce Host-Dependent Disease Tolerance to Infection
Article Snippet: .. Targeted metabolomics Each sample was injected onto a SeQuant ZIC-pHILIC HPLC column (Merck, 100 × 2.1 mm; 5 μm) operated with an Ultimate 3000 HPLC system (Dionex, Thermo Fisher Scientific) at a flow rate of 100 μL/min and directly coupled to a TSQ Quantiva mass spectrometer (Thermo Fisher Scientific). ..

Article Title: Protective Role of Mytilus edulis Hydrolysate in Lipopolysaccharide-Galactosamine Acute Liver Injury
Article Snippet: The sustainability of the lab-scale pre-GMP production was confirmed by the IGMM (Institute of Genetics and Molecular Medicine) Mass Spectrometry laboratory (University of Edinburgh). .. N2-01 (in its undiluted form) was diluted 40:1 in 5:3:2 methanol:acetonitrile:water and 10 ul were injected onto a ZIC-pHILIC 4.6 mm x 150 mm Sequant column (Merck, United Kingdom) on an Ultimate 3000 series HPLC (Thermo Fisher Scientific, United States) with A gradient from 90 to 5% B in 20 min, where A was 20 mm ammonium carbonate and B was acetonitrile. .. Data were acquired on Q Exactive (Thermo Fisher Scientific, United States) with HESI in positive (75.5–1132.5) and negative (77.5–1132.5) mode at 70k resolution, with ddMS2 at 17.5k.

High Performance Liquid Chromatography:

Article Title: Host-dependent induction of disease tolerance to infection by tetracycline antibiotics
Article Snippet: Compound annotation was conducted by searching the mzCloud database with a mass accuracy of 3 ppm for precursor masses and 10 ppm for fragment ion masses as well as ChemSpider with a mass accuracy of 3 ppm using BioCyc, Human Metabolome Database, KEGG, MassBank and MetaboLights as databases. .. Targeted metabolomics Each sample was injected onto a SeQuant ZIC-pHILIC HPLC column (Merck, 100 x 2.1 mm; 5 µm) operated with an Ultimate 3000 HPLC system (Dionex, Thermo Fisher Scientific) at a flow rate of 100 µl/min and directly coupled to a TSQ Quantiva mass spectrometer (Thermo Fisher Scientific). ..

Article Title: Tetracycline Antibiotics Induce Host-Dependent Disease Tolerance to Infection
Article Snippet: .. Targeted metabolomics Each sample was injected onto a SeQuant ZIC-pHILIC HPLC column (Merck, 100 × 2.1 mm; 5 μm) operated with an Ultimate 3000 HPLC system (Dionex, Thermo Fisher Scientific) at a flow rate of 100 μL/min and directly coupled to a TSQ Quantiva mass spectrometer (Thermo Fisher Scientific). ..

Article Title: Metabolic Analyses Revealed Time-Dependent Synergistic Killing by Colistin and Aztreonam Combination Against Multidrug-Resistant Acinetobacter baumannii
Article Snippet: The supernatants (0.3 mL) containing extracted metabolites were collected after centrifugation at 3,220 ×g and 4°C for 10 min, then further centrifuged at 14,000 ×g for 10 min to achieve particle-free samples (0.2 mL) for LC-MS analysis. .. LC-MS Analysis of Metabolites Based on a published method , metabolite samples were analyzed on a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled to a Dionex U3000 high-performance liquid chromatography (HPLC) with a ZIC-pHILIC column (5 μm, polymeric, 150 × 4.6 mm; SeQuant, Merck). .. The MS system was operated in both positive and negative electro-spray ionisation (ESI) mode with a resolution at 35,000 and a detection range of 85 to 1,275 m/z .

Article Title: Protective Role of Mytilus edulis Hydrolysate in Lipopolysaccharide-Galactosamine Acute Liver Injury
Article Snippet: The sustainability of the lab-scale pre-GMP production was confirmed by the IGMM (Institute of Genetics and Molecular Medicine) Mass Spectrometry laboratory (University of Edinburgh). .. N2-01 (in its undiluted form) was diluted 40:1 in 5:3:2 methanol:acetonitrile:water and 10 ul were injected onto a ZIC-pHILIC 4.6 mm x 150 mm Sequant column (Merck, United Kingdom) on an Ultimate 3000 series HPLC (Thermo Fisher Scientific, United States) with A gradient from 90 to 5% B in 20 min, where A was 20 mm ammonium carbonate and B was acetonitrile. .. Data were acquired on Q Exactive (Thermo Fisher Scientific, United States) with HESI in positive (75.5–1132.5) and negative (77.5–1132.5) mode at 70k resolution, with ddMS2 at 17.5k.

Mass Spectrometry:

Article Title: Host-dependent induction of disease tolerance to infection by tetracycline antibiotics
Article Snippet: Compound annotation was conducted by searching the mzCloud database with a mass accuracy of 3 ppm for precursor masses and 10 ppm for fragment ion masses as well as ChemSpider with a mass accuracy of 3 ppm using BioCyc, Human Metabolome Database, KEGG, MassBank and MetaboLights as databases. .. Targeted metabolomics Each sample was injected onto a SeQuant ZIC-pHILIC HPLC column (Merck, 100 x 2.1 mm; 5 µm) operated with an Ultimate 3000 HPLC system (Dionex, Thermo Fisher Scientific) at a flow rate of 100 µl/min and directly coupled to a TSQ Quantiva mass spectrometer (Thermo Fisher Scientific). ..

Article Title: Tetracycline Antibiotics Induce Host-Dependent Disease Tolerance to Infection
Article Snippet: .. Targeted metabolomics Each sample was injected onto a SeQuant ZIC-pHILIC HPLC column (Merck, 100 × 2.1 mm; 5 μm) operated with an Ultimate 3000 HPLC system (Dionex, Thermo Fisher Scientific) at a flow rate of 100 μL/min and directly coupled to a TSQ Quantiva mass spectrometer (Thermo Fisher Scientific). ..

Article Title: Metabolic Analyses Revealed Time-Dependent Synergistic Killing by Colistin and Aztreonam Combination Against Multidrug-Resistant Acinetobacter baumannii
Article Snippet: The supernatants (0.3 mL) containing extracted metabolites were collected after centrifugation at 3,220 ×g and 4°C for 10 min, then further centrifuged at 14,000 ×g for 10 min to achieve particle-free samples (0.2 mL) for LC-MS analysis. .. LC-MS Analysis of Metabolites Based on a published method , metabolite samples were analyzed on a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled to a Dionex U3000 high-performance liquid chromatography (HPLC) with a ZIC-pHILIC column (5 μm, polymeric, 150 × 4.6 mm; SeQuant, Merck). .. The MS system was operated in both positive and negative electro-spray ionisation (ESI) mode with a resolution at 35,000 and a detection range of 85 to 1,275 m/z .

Liquid Chromatography:

Article Title: Pyrimidine Salvage in Trypanosoma brucei Bloodstream Forms and the Trypanocidal Action of Halogenated Pyrimidiness
Article Snippet: Metabolomics samples were analyzed using hydrophilic interaction liquid chromatography coupled with high-resolution mass spectrometry. .. Liquid chromatography separation used a zwitterionic ZIC-pHILIC column (Merck Sequant, Umeå, Sweden) with ammonium carbonate alkaline gradient, as previously described ( ). .. The method was performed on a Dionex RSLC3000 (Thermo Fisher, Waltham, MA) LC system coupled with an Exactive Orbitrap (Thermo Fisher) operating at 50,000 resolution in positive and negative mode ESI (rapid switching) with mass spectrometry parameters as previously published ( ).

Liquid Chromatography with Mass Spectroscopy:

Article Title: Metabolic Analyses Revealed Time-Dependent Synergistic Killing by Colistin and Aztreonam Combination Against Multidrug-Resistant Acinetobacter baumannii
Article Snippet: The supernatants (0.3 mL) containing extracted metabolites were collected after centrifugation at 3,220 ×g and 4°C for 10 min, then further centrifuged at 14,000 ×g for 10 min to achieve particle-free samples (0.2 mL) for LC-MS analysis. .. LC-MS Analysis of Metabolites Based on a published method , metabolite samples were analyzed on a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled to a Dionex U3000 high-performance liquid chromatography (HPLC) with a ZIC-pHILIC column (5 μm, polymeric, 150 × 4.6 mm; SeQuant, Merck). .. The MS system was operated in both positive and negative electro-spray ionisation (ESI) mode with a resolution at 35,000 and a detection range of 85 to 1,275 m/z .

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    Merck & Co sequant zic philic column
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic column/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck & Co zwitterionic zic philic column
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Zwitterionic Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zwitterionic zic philic column/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zwitterionic zic philic column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck & Co zic philic column
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )

    Journal: BMC Microbiology

    Article Title: Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG

    doi: 10.1186/s12866-020-02006-z

    Figure Lengend Snippet: Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )

    Article Snippet: Five-microliters of each sample were analysed immediately by LS-MS run in negative ion mode as described previously [ ] in order to ensure the efficient separation of GlcNAc-MurNAc and MurNAc on a SeQuant® ZIC®-pHILIC column (Merck, PEEK 150 × 2.1 mm, 5 μm).

    Techniques: Incubation