sequalprep long range pcr kit (Thermo Fisher)


93
Name:
SequalPrep Long PCR Kit
Description:
The SequalPrep Long PCR Kit with dNTPs provides the highest amplification success rate of any long range PCR kit tested across multiple organisms and platforms for non optimized amplicons up to 20 kB with little to no optimization Successful long range PCR with minimal optimization enables efficient PCR enrichment for a variety of applications including medical re sequencing on next generation sequencing NGS platforms PCR amplification is the traditional method to enrich target regions of the genome for genotyping and re sequencing applications To take advantage of the enhanced NGS processing capabilities higher throughput enrichment methods have become necessary Unfortunately the potential efficiency gains from long range PCR using available commercial solutions have been offset with lower amplification success rates limiting enrichment to 5 kB regions The SequalPrep Long PCR Kit with dNTPs overcomes these issues and provides the highest amplification success rate of any commercially available kit tested across multiple organisms and platforms for amplicons up to 20 kB with minimal optimization This kit can be used upstream of any NGS platform or for other genotyping applications When combined with the SequalPrep Normalization Plate the SequalPrep Sequence Equalization Sample Preparation System is the first complete PCR enrichment and amplicon equalization solution that provides a viable and efficient enrichment method to meet NGS re sequencing throughput capabilities This NGS Sample Prep system significantly reduces PCR amplification and normalization complexity to allow for enrichment of regions up to 1MB in a 96 well plate format Amplify and equalize with the ease of SequalPrep for consistently high coverage of your regions of interest
Catalog Number:
a10498
Price:
None
Category:
Proteins Enzymes Peptides
Applications:
DNA Sequencing|Kits & Reagents for Sanger Sequencing|Long Fragment PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes|SOLiD® Next-Generation Sequencing|SOLiD® Next-Generation Sequencing Sample Enrichment|Sample & Library Preparation for SOLiD® Next-Generation Sequencing|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Targeted Sequencing|Sequencing
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Structured Review
Thermo Fisher
sequalprep long range pcr kit
The SequalPrep Long PCR Kit with dNTPs provides the highest amplification success rate of any long range PCR kit tested across multiple organisms and platforms for non optimized amplicons up to 20 kB with little to no optimization Successful long range PCR with minimal optimization enables efficient PCR enrichment for a variety of applications including medical re sequencing on next generation sequencing NGS platforms PCR amplification is the traditional method to enrich target regions of the genome for genotyping and re sequencing applications To take advantage of the enhanced NGS processing capabilities higher throughput enrichment methods have become necessary Unfortunately the potential efficiency gains from long range PCR using available commercial solutions have been offset with lower amplification success rates limiting enrichment to 5 kB regions The SequalPrep Long PCR Kit with dNTPs overcomes these issues and provides the highest amplification success rate of any commercially available kit tested across multiple organisms and platforms for amplicons up to 20 kB with minimal optimization This kit can be used upstream of any NGS platform or for other genotyping applications When combined with the SequalPrep Normalization Plate the SequalPrep Sequence Equalization Sample Preparation System is the first complete PCR enrichment and amplicon equalization solution that provides a viable and efficient enrichment method to meet NGS re sequencing throughput capabilities This NGS Sample Prep system significantly reduces PCR amplification and normalization complexity to allow for enrichment of regions up to 1MB in a 96 well plate format Amplify and equalize with the ease of SequalPrep for consistently high coverage of your regions of interest
https://www.bioz.com/result/sequalprep long range pcr kit/product/Thermo Fisher
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
The SequalPrep Long PCR Kit with dNTPs provides the highest amplification success rate of any long range PCR kit tested across multiple organisms and platforms for non optimized amplicons up to 20 kB with little to no optimization Successful long range PCR with minimal optimization enables efficient PCR enrichment for a variety of applications including medical re sequencing on next generation sequencing NGS platforms PCR amplification is the traditional method to enrich target regions of the genome for genotyping and re sequencing applications To take advantage of the enhanced NGS processing capabilities higher throughput enrichment methods have become necessary Unfortunately the potential efficiency gains from long range PCR using available commercial solutions have been offset with lower amplification success rates limiting enrichment to 5 kB regions The SequalPrep Long PCR Kit with dNTPs overcomes these issues and provides the highest amplification success rate of any commercially available kit tested across multiple organisms and platforms for amplicons up to 20 kB with minimal optimization This kit can be used upstream of any NGS platform or for other genotyping applications When combined with the SequalPrep Normalization Plate the SequalPrep Sequence Equalization Sample Preparation System is the first complete PCR enrichment and amplicon equalization solution that provides a viable and efficient enrichment method to meet NGS re sequencing throughput capabilities This NGS Sample Prep system significantly reduces PCR amplification and normalization complexity to allow for enrichment of regions up to 1MB in a 96 well plate format Amplify and equalize with the ease of SequalPrep for consistently high coverage of your regions of interest
https://www.bioz.com/result/sequalprep long range pcr kit/product/Thermo Fisher
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sequalprep long range pcr kit - by Bioz Stars,
2021-03
93/100 stars
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Polymerase Chain Reaction:Article Title: Probiotic supplements prevented oxonic acid-induced hyperuricemia and renal damage Article Snippet: In brief, amplicons were generated using primers that target approximately 400 base pairs of the V3V4 variable region of the 16S rRNA gene. .. PCR products were normalized using a Article Title: Helicase-like transcription factor (Hltf) gene-deletion promotes oxidative phosphorylation (OXPHOS) in colorectal tumors of AOM/DSS-treated mice Article Snippet: Isolation of genomic DNA from tail biopsies and tumor samples was achieved with the DNeasy Blood & Tissue Kit (69506) purchased from Qiagen (Valencia, CA). .. Applied Biosystems TaqMan® MGB (minor groove binder) probes with a 5´ reporter (FAM or VIC) and a 3´ nonfluorescent quencher (MGB-NFQ), Invitrogen™ TRIzol™ Reagent (15596026), Invitrogen SuperScript™ Vilo™ cDNA Synthesis Kit (11754050), and SequalPrep™ Long PCR Kit with Article Title: An Application of NGS for WDR36 Gene in Taiwanese Patients with Juvenile-Onset Open-Angle Glaucoma Article Snippet: Long range polymerase chain reaction (PCR) was applied for enrichment of WDR36 gene, intragenic primer sequences shown in Table . .. Cycling profile of long range PCR was conducted as follows: one cycle at 94℃ for 2 min, followed by 10 cycles at 94℃ for 10 s, at 55℃ for 30 s, and at 68℃ for 14 min, followed by 30 cycles at 94℃ for 10 s, at 55℃ for 30 s, and at 68℃ for 14 min (+20 s every cycle), followed by a final extension step at 72℃ for 5 min, according to Article Title: Conditional Disruption of Hepatic Carbamoyl Phosphate Synthetase 1 in Mice Results in Hyperammonemia without Orotic Aciduria and Can be Corrected by Liver-Directed Gene Therapy Article Snippet: Twelve passing clones were then expanded and DNA extracted via Qiagen blood and tissue kit following manufacturer’s protocol. .. Approximately 50ng of DNA was tested by long range PCR across the 5′ arm as well as the 3′ distal loxP using Article Title: Hybridization capture of larch (Larix Mill) chloroplast genomes from sedimentary ancient DNA reveals past changes of Siberian forests Article Snippet: Long-range PCR products were generated from DNA extractions from needles of a L. gmelinii individual collected in the Botanical Gardens of the University of Potsdam (Accession number XX-0POTSD-3867). .. PCR amplification was conducted using the Article Title: Analysis of the chromosome X exome in patients with autism spectrum disorders identified novel candidate genes, including TMLHE Article Snippet: PCR products were quantified on a Caliper LabChip system (Caliper Life Sciences, Hopkinton, MA, USA). .. In addition, the presence of the deletion of exon 2 in TMLHE was confirmed by long-range PCR using the Article Title: Large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in Vietnam Article Snippet: Then, 1 μl of the first-round reaction was used as template for the second-round PCR with amplification (using GoTaq DNA polymerase; Promega) conditions as follows: 95 °C for 5 min, and 30 cycles of denaturation (94 °C, 30 s), annealing (50 °C, 30 s) and elongation (72 °C, 60 s) in a thermal cycler. .. A Article Title: A higher throughput assay for quantification of melphalan-induced DNA damage in peripheral blood mononuclear cells Article Snippet: .. LA Taq DNA Polymerase (TaKaRa, Japan), Ex Taq Hot Start Version Kit (TaKaRa, Japan), and Purification:Article Title: Probiotic supplements prevented oxonic acid-induced hyperuricemia and renal damage Article Snippet: In brief, amplicons were generated using primers that target approximately 400 base pairs of the V3V4 variable region of the 16S rRNA gene. .. PCR products were normalized using a Size-exclusion Chromatography:Article Title: Conditional Disruption of Hepatic Carbamoyl Phosphate Synthetase 1 in Mice Results in Hyperammonemia without Orotic Aciduria and Can be Corrected by Liver-Directed Gene Therapy Article Snippet: Twelve passing clones were then expanded and DNA extracted via Qiagen blood and tissue kit following manufacturer’s protocol. .. Approximately 50ng of DNA was tested by long range PCR across the 5′ arm as well as the 3′ distal loxP using Amplification:Article Title: Hybridization capture of larch (Larix Mill) chloroplast genomes from sedimentary ancient DNA reveals past changes of Siberian forests Article Snippet: Long-range PCR products were generated from DNA extractions from needles of a L. gmelinii individual collected in the Botanical Gardens of the University of Potsdam (Accession number XX-0POTSD-3867). .. PCR amplification was conducted using the Article Title: A higher throughput assay for quantification of melphalan-induced DNA damage in peripheral blood mononuclear cells Article Snippet: .. LA Taq DNA Polymerase (TaKaRa, Japan), Ex Taq Hot Start Version Kit (TaKaRa, Japan), and |