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Waters Corporation seppak c18 solid phase extraction spe cartridges
Seppak C18 Solid Phase Extraction Spe Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/seppak c18 solid phase extraction spe cartridges/product/Waters Corporation
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
seppak c18 solid phase extraction spe cartridges - by Bioz Stars, 2020-04
91/100 stars

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trifluoroacetic acid tfa

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Size-exclusion Chromatography:

Article Title: Valosin-containing protein (VCP)–Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor *
Article Snippet: Paragraph title: Protein Digestion of SEC Fractions ... Samples were acidified with 0.1% trifluoroacetic acid (TFA), then desalted using SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA).

Labeling:

Article Title: Custom 4-Plex DiLeu Isobaric Labels Enable Relative Quantification of Urinary Proteins in Men with Lower Urinary Tract Symptoms (LUTS)
Article Snippet: .. Peptides were desalted with SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA) according to the manufacturer’s protocol, concentrated, and reconstituted in 0.5 M TEAB before labeling. .. Urinary Peptide Labeling with 4-plex DiLeu Human urine peptides were labeled with a 5x w /w excess of DiLeu.

Concentration Assay:

Article Title: Custom 4-Plex DiLeu Isobaric Labels Enable Relative Quantification of Urinary Proteins in Men with Lower Urinary Tract Symptoms (LUTS)
Article Snippet: Protein samples were then diluted with 50 mM Tris HCl until reaching a urea concentration of < 1 M before adding trypsin in a 50:1 protein:enzyme ratio. .. Peptides were desalted with SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA) according to the manufacturer’s protocol, concentrated, and reconstituted in 0.5 M TEAB before labeling.

Article Title: Valosin-containing protein (VCP)–Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor *
Article Snippet: To mitigate significant nontryptic peptide activity, urea was added to each fraction prior to thawing for a final concentration of 8 m . .. Samples were acidified with 0.1% trifluoroacetic acid (TFA), then desalted using SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA).

Incubation:

Article Title: Valosin-containing protein (VCP)–Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor *
Article Snippet: Samples were sonicated until urea was completely dissolved, then incubated with 10 m m tris(2-carboxyethyl)phosphine (TCEP) and 40 m m chloroacetamide (CAA) at room temperature for 10 min. Fractions were then pooled by threes, to create 12 final fractions per replicate. .. Samples were acidified with 0.1% trifluoroacetic acid (TFA), then desalted using SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA).

Activity Assay:

Article Title: Valosin-containing protein (VCP)–Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor *
Article Snippet: To mitigate significant nontryptic peptide activity, urea was added to each fraction prior to thawing for a final concentration of 8 m . .. Samples were acidified with 0.1% trifluoroacetic acid (TFA), then desalted using SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA).

Sonication:

Article Title: Valosin-containing protein (VCP)–Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor *
Article Snippet: Samples were sonicated until urea was completely dissolved, then incubated with 10 m m tris(2-carboxyethyl)phosphine (TCEP) and 40 m m chloroacetamide (CAA) at room temperature for 10 min. Fractions were then pooled by threes, to create 12 final fractions per replicate. .. Samples were acidified with 0.1% trifluoroacetic acid (TFA), then desalted using SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA).

Cellular Antioxidant Activity Assay:

Article Title: Valosin-containing protein (VCP)–Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor *
Article Snippet: Samples were sonicated until urea was completely dissolved, then incubated with 10 m m tris(2-carboxyethyl)phosphine (TCEP) and 40 m m chloroacetamide (CAA) at room temperature for 10 min. Fractions were then pooled by threes, to create 12 final fractions per replicate. .. Samples were acidified with 0.1% trifluoroacetic acid (TFA), then desalted using SepPak C18 solid-phase extraction (SPE) cartridges (Waters, Milford, MA).

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    Waters Corporation solid phase extraction spe cartridges seppak c18 plus
    Solid Phase Extraction Spe Cartridges Seppak C18 Plus, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction spe cartridges seppak c18 plus/product/Waters Corporation
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction spe cartridges seppak c18 plus - by Bioz Stars, 2020-04
    86/100 stars
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    96
    Waters Corporation c18 solid phase extraction spe cartridge
    C18 Solid Phase Extraction Spe Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 solid phase extraction spe cartridge/product/Waters Corporation
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    c18 solid phase extraction spe cartridge - by Bioz Stars, 2020-04
    96/100 stars
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    94
    Waters Corporation solid phase extraction spe cartridges
    Solid Phase Extraction Spe Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction spe cartridges/product/Waters Corporation
    Average 94 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction spe cartridges - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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