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Waters Corporation seppak c18 solid phase extraction cartridges
Seppak C18 Solid Phase Extraction Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/seppak c18 solid phase extraction cartridges/product/Waters Corporation
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
seppak c18 solid phase extraction cartridges - by Bioz Stars, 2020-04
87/100 stars

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Centrifugation:

Article Title: An Erk/Cdk5 axis controls the diabetogenic actions of PPARγ
Article Snippet: Following incubation, beads were recovered by centrifugation (4 min, 200 × g) and washed with binding buffer (5 × 200 μL). .. SepPak C18 solid-phase extraction cartridges were purchased from Waters Corporation (Milford, MA).

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
Article Snippet: .. Insoluble material was removed by centrifugation at 1989 g for 10 min. Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters). ..

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.
Article Snippet: Insoluble material was removed by centrifugation at 1989g for 10 minutes. .. 570 Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Modification:

Article Title: SPECHT – Single-stage phosphopeptide enrichment and stable-isotope chemical tagging: Quantitative phosphoproteomics of insulin action in muscle
Article Snippet: SepPak C18 solid-phase extraction cartridges and Oasis HLB vacuum extraction plates were from Waters Corporation (Milford, MA). .. Dulbecco’s modified Eagle’s medium (DMEM), PBS, penicillin and streptomycin were from Cellgro Mediatech Inc (Manassas, VA).

Article Title: An Erk/Cdk5 axis controls the diabetogenic actions of PPARγ
Article Snippet: ActivX ATP probes, high capacity binding streptavidin and TMT (tandem mass tags) were obtained from Thermo Scientific (Rockford, IL), modified trypsin was obtained from Promega (Madison, WI), and TiO2 beads were obtained from GL Sciences (Tokyo, Japan). .. SepPak C18 solid-phase extraction cartridges were purchased from Waters Corporation (Milford, MA).

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
Article Snippet: Samples were incubated with 100 µg of sequencing grade modified trypsin (Promega) and incubated at room temperature with rotation for 20 hr. .. Insoluble material was removed by centrifugation at 1989 g for 10 min. Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.
Article Snippet: Samples were incubated with 100 µg of sequencing grade 566 modified trypsin (Promega) and incubated at room temperature with rotation for 20 hours. .. 570 Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases
Article Snippet: Modified trypsin was from Promega (Madison, WI); Glu-C protease was from Worthington Biochemicals (Lakewood, NJ). .. SepPak C18 solid-phase extraction cartridges and Oasis HLB vacuum extraction plates were from Waters Corporation (Milford, MA).

Purification:

Article Title: An Erk/Cdk5 axis controls the diabetogenic actions of PPARγ
Article Snippet: The purified phosphopeptides were resuspended in 8 μL 5% formic acid (FA) and 4 μL was injected and analyzed by LC-MS3. .. SepPak C18 solid-phase extraction cartridges were purchased from Waters Corporation (Milford, MA).

Acid Assay:

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.
Article Snippet: Paragraph title: 30 minutes. A bicinchoninic acid assay (Pierce) was performed to measure protein concentration 560 ... 570 Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Concentration Assay:

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
Article Snippet: The sample pH was reduced to approximately 2.0 by the addition of 10% trifluoroacetic acid (TFA) to a final concentration of 0.3% trifluoroacetic acid followed by 6 M HCl at a 1:100 vol ratio. .. Insoluble material was removed by centrifugation at 1989 g for 10 min. Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.
Article Snippet: The 567 sample pH was reduced to approximately 2.0 by the addition of 10% trifluoroacetic acid (TFA) 568 to a final concentration of 0.3% trifluoroacetic acid followed by 6 M HCl at a 1:100 volume 569 ratio. .. 570 Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Incubation:

Article Title: An Erk/Cdk5 axis controls the diabetogenic actions of PPARγ
Article Snippet: Following incubation, beads were recovered by centrifugation (4 min, 200 × g) and washed with binding buffer (5 × 200 μL). .. SepPak C18 solid-phase extraction cartridges were purchased from Waters Corporation (Milford, MA).

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
Article Snippet: Samples were incubated with 100 µg of sequencing grade modified trypsin (Promega) and incubated at room temperature with rotation for 20 hr. .. Insoluble material was removed by centrifugation at 1989 g for 10 min. Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.
Article Snippet: Samples were incubated with 100 µg of sequencing grade 566 modified trypsin (Promega) and incubated at room temperature with rotation for 20 hours. .. 570 Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Mass Spectrometry:

Article Title: An Erk/Cdk5 axis controls the diabetogenic actions of PPARγ
Article Snippet: Paragraph title: Mass Spectrometry ... SepPak C18 solid-phase extraction cartridges were purchased from Waters Corporation (Milford, MA).

Protein Concentration:

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.
Article Snippet: Paragraph title: 30 minutes. A bicinchoninic acid assay (Pierce) was performed to measure protein concentration 560 ... 570 Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Sequencing:

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
Article Snippet: Samples were incubated with 100 µg of sequencing grade modified trypsin (Promega) and incubated at room temperature with rotation for 20 hr. .. Insoluble material was removed by centrifugation at 1989 g for 10 min. Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Article Title: Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages.
Article Snippet: Samples were incubated with 100 µg of sequencing grade 566 modified trypsin (Promega) and incubated at room temperature with rotation for 20 hours. .. 570 Peptides were desalted using SepPak C18 solid-phase extraction cartridges (Waters).

Injection:

Article Title: An Erk/Cdk5 axis controls the diabetogenic actions of PPARγ
Article Snippet: The purified phosphopeptides were resuspended in 8 μL 5% formic acid (FA) and 4 μL was injected and analyzed by LC-MS3. .. SepPak C18 solid-phase extraction cartridges were purchased from Waters Corporation (Milford, MA).

Binding Assay:

Article Title: An Erk/Cdk5 axis controls the diabetogenic actions of PPARγ
Article Snippet: ActivX ATP probes, high capacity binding streptavidin and TMT (tandem mass tags) were obtained from Thermo Scientific (Rockford, IL), modified trypsin was obtained from Promega (Madison, WI), and TiO2 beads were obtained from GL Sciences (Tokyo, Japan). .. SepPak C18 solid-phase extraction cartridges were purchased from Waters Corporation (Milford, MA).

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  • 98
    Waters Corporation solid phase extraction
    Solid Phase Extraction, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 98/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction/product/Waters Corporation
    Average 98 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    94
    Waters Corporation solid phase extraction cartridges
    Solid Phase Extraction Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid phase extraction cartridges/product/Waters Corporation
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    solid phase extraction cartridges - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    92
    Waters Corporation seppak c18 solid phase extraction cartridge
    Seppak C18 Solid Phase Extraction Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/seppak c18 solid phase extraction cartridge/product/Waters Corporation
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    seppak c18 solid phase extraction cartridge - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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