sepharose cl 4b  (Millipore)


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    Name:
    Sepharose CL 4B
    Description:
    CL4B200 1L s update product number is GE17 0150 01
    Catalog Number:
    cl4b200
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    Structured Review

    Millipore sepharose cl 4b
    SDS-PAGE analysis of the salivary mucus eluted in the V o of the <t>Sepharose</t> <t>CL-4B</t> gel filtration column. Freeze-dried material (30 μg) of the V o prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.
    CL4B200 1L s update product number is GE17 0150 01
    https://www.bioz.com/result/sepharose cl 4b/product/Millipore
    Average 98 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    sepharose cl 4b - by Bioz Stars, 2020-08
    98/100 stars

    Images

    1) Product Images from "The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay"

    Article Title: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

    Journal: Virology Journal

    doi: 10.1186/1743-422X-3-99

    SDS-PAGE analysis of the salivary mucus eluted in the V o of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V o prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.
    Figure Legend Snippet: SDS-PAGE analysis of the salivary mucus eluted in the V o of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V o prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.

    Techniques Used: SDS Page, Filtration, Staining, Molecular Weight, Marker

    SDS-PAGE analysis of the salivary mucus eluted in the V i of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V i prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.
    Figure Legend Snippet: SDS-PAGE analysis of the salivary mucus eluted in the V i of the Sepharose CL-4B gel filtration column. Freeze-dried material (30 μg) of the V i prepared in reducing gel loading buffer was separated by 10% SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (a) and PAS (b). The molecular weight marker is indicated by Mw and lanes 1 to 6 represents samples from 6 donors.

    Techniques Used: SDS Page, Filtration, Staining, Molecular Weight, Marker

    Sepharose CL-4B gel filtration of crude saliva. An aliquot (20 ml) of soluble human crude saliva extracted in 6 M GuHCl containing 10 mM EDTA, 5 mM NEM, 1 mM PMSF and 0.1% CHAPS pH 6.5 was chromatographed and eluted with 4 M GuHCl containing 10 mM EDTA, 5 mM NEM and 0.05% CHAPS pH 6.5 at flow rate of 48 ml/h at room temperature. Fractions were analysed for carbohydrate with PAS at 555 nm (◆) and for protein A 280 (■). Materials eluted in the void volume (V o ) and included volume (V i ) were pooled separately, dialysed against three changes of distilled water for overnight at 4°C and freeze-dried.
    Figure Legend Snippet: Sepharose CL-4B gel filtration of crude saliva. An aliquot (20 ml) of soluble human crude saliva extracted in 6 M GuHCl containing 10 mM EDTA, 5 mM NEM, 1 mM PMSF and 0.1% CHAPS pH 6.5 was chromatographed and eluted with 4 M GuHCl containing 10 mM EDTA, 5 mM NEM and 0.05% CHAPS pH 6.5 at flow rate of 48 ml/h at room temperature. Fractions were analysed for carbohydrate with PAS at 555 nm (◆) and for protein A 280 (■). Materials eluted in the void volume (V o ) and included volume (V i ) were pooled separately, dialysed against three changes of distilled water for overnight at 4°C and freeze-dried.

    Techniques Used: Filtration, Flow Cytometry

    2) Product Images from "Quantification of small extracellular vesicles by size exclusion chromatography with fluorescence detection"

    Article Title: Quantification of small extracellular vesicles by size exclusion chromatography with fluorescence detection

    Journal: Analytical chemistry

    doi: 10.1021/acs.analchem.6b03348

    SEC-FD chromatograms obtained from separating: (a) sequentially diluted solutions of CM-Dil labeled 100-nm liposomes, and (b) a solution of albumin-FITC conjugate on an in-house prepared Sepharose CL-4B column.
    Figure Legend Snippet: SEC-FD chromatograms obtained from separating: (a) sequentially diluted solutions of CM-Dil labeled 100-nm liposomes, and (b) a solution of albumin-FITC conjugate on an in-house prepared Sepharose CL-4B column.

    Techniques Used: Size-exclusion Chromatography, Labeling

    3) Product Images from "Preparation by alkaline treatment and detailed characterisation of empty hepatitis B virus core particles for vaccine and gene therapy applications"

    Article Title: Preparation by alkaline treatment and detailed characterisation of empty hepatitis B virus core particles for vaccine and gene therapy applications

    Journal: Scientific Reports

    doi: 10.1038/srep11639

    Separation of alkaline-treated HBc VLPs by Sepharose CL-4B column chromatography . ( a ) Chromatography of bacteria- and yeast-produced wt HBc VLPs as well as two representative lysine-exposing HBc VLP variants: HBc-K75 and HBc-K80. Pooled fractions are marked by capital letters. ( b ) DLS (left) and EM (right) characterisation of the fractions A of the alkaline-treated HBc VLP variants: bacteria-produced wt HBc (top), HBc-K77 (middle), HBc-K79 (bottom). Three independent DLS measurements are shown, mean particle diameters are indicated by numbers on the respective DLS graphs.
    Figure Legend Snippet: Separation of alkaline-treated HBc VLPs by Sepharose CL-4B column chromatography . ( a ) Chromatography of bacteria- and yeast-produced wt HBc VLPs as well as two representative lysine-exposing HBc VLP variants: HBc-K75 and HBc-K80. Pooled fractions are marked by capital letters. ( b ) DLS (left) and EM (right) characterisation of the fractions A of the alkaline-treated HBc VLP variants: bacteria-produced wt HBc (top), HBc-K77 (middle), HBc-K79 (bottom). Three independent DLS measurements are shown, mean particle diameters are indicated by numbers on the respective DLS graphs.

    Techniques Used: Column Chromatography, Chromatography, Produced

    4) Product Images from "Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods"

    Article Title: Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0145686

    Efficiency and selectivity of size exclusion chromatography (SEC) on exosome isolation performed with various matrices. (A) CD63, TSG101 and albumin content of different fractions collected during SEC on Sepharose 2B (top), Sepharose CL-4B (middle) and Sephacryl S-400 (bottom) columns with equal volumes (left column) or equal protein amounts of fractions (right column) loaded for Western blot. (B) Size distribution of particles isolated with various SEC matrices evaluated with dynamic light scattering. Sepharose 2B (top), Sepharose CL-4B (middle) and Sephacryl S-400 (bottom) columns.
    Figure Legend Snippet: Efficiency and selectivity of size exclusion chromatography (SEC) on exosome isolation performed with various matrices. (A) CD63, TSG101 and albumin content of different fractions collected during SEC on Sepharose 2B (top), Sepharose CL-4B (middle) and Sephacryl S-400 (bottom) columns with equal volumes (left column) or equal protein amounts of fractions (right column) loaded for Western blot. (B) Size distribution of particles isolated with various SEC matrices evaluated with dynamic light scattering. Sepharose 2B (top), Sepharose CL-4B (middle) and Sephacryl S-400 (bottom) columns.

    Techniques Used: Size-exclusion Chromatography, Isolation, Western Blot

    5) Product Images from "Serglycin Is Implicated in the Promotion of Aggressive Phenotype of Breast Cancer Cells"

    Article Title: Serglycin Is Implicated in the Promotion of Aggressive Phenotype of Breast Cancer Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0078157

    Fractionation of PGs secreted from the MDA-MB-231 breast cancer cell line by anion-exchange and gel permeation chromatographies. One liter of conditioned medium was fractionated on a DEAE-Sephacel column (40 ml bed volume). The column was eluted stepwise with 3 volumes of the formamide buffer described under “Experimental Procedures” containing 0.2 M NaCl and 10 volumes of a NaCl linear gradient ranging from 0.2 to 1.0 M NaCl. Fractions of 6.7 ml were collected, and aliquots were precipitated by the addition of ethanol in the presence of potassium acetate. Precipitates were dissolved in distilled water, analyzed for their GAG content by the DMMB method (A), and subjected to SDS-PAGE analysis using a 4% stacking - 10% separating gel (B). Gels were stained with toluidine blue, followed by staining with coomassie blue. Serglycin was detected throughout the dissolved aliquots by Western blotting analysis after chondroitinase ABC digestion using a polyclonal antibody against serglycin (C) (SG, standard of serglycin used as positive control). A major PG peak was eluted from 0.50 to 0.7 M NaCl and pooled as indicated by the bar. Pooled serglycin was further fractionated by gel permeation chromatography (D). The pooled fractions were chromatographed on a Sepharose CL-4B column. The column was eluted with 4 M guanidine hydrochloride, 50 mM sodium acetate buffer, pH 5.8. Fractions were collected, and PGs were monitored by the DMMB method (D), were subjected to SDS-PAGE following toluidine blue and coomassie staining (E), and were analyzed by Western blotting after chondroitinase ABC digestion (F) (SG, standard of serglycin used as positive control).
    Figure Legend Snippet: Fractionation of PGs secreted from the MDA-MB-231 breast cancer cell line by anion-exchange and gel permeation chromatographies. One liter of conditioned medium was fractionated on a DEAE-Sephacel column (40 ml bed volume). The column was eluted stepwise with 3 volumes of the formamide buffer described under “Experimental Procedures” containing 0.2 M NaCl and 10 volumes of a NaCl linear gradient ranging from 0.2 to 1.0 M NaCl. Fractions of 6.7 ml were collected, and aliquots were precipitated by the addition of ethanol in the presence of potassium acetate. Precipitates were dissolved in distilled water, analyzed for their GAG content by the DMMB method (A), and subjected to SDS-PAGE analysis using a 4% stacking - 10% separating gel (B). Gels were stained with toluidine blue, followed by staining with coomassie blue. Serglycin was detected throughout the dissolved aliquots by Western blotting analysis after chondroitinase ABC digestion using a polyclonal antibody against serglycin (C) (SG, standard of serglycin used as positive control). A major PG peak was eluted from 0.50 to 0.7 M NaCl and pooled as indicated by the bar. Pooled serglycin was further fractionated by gel permeation chromatography (D). The pooled fractions were chromatographed on a Sepharose CL-4B column. The column was eluted with 4 M guanidine hydrochloride, 50 mM sodium acetate buffer, pH 5.8. Fractions were collected, and PGs were monitored by the DMMB method (D), were subjected to SDS-PAGE following toluidine blue and coomassie staining (E), and were analyzed by Western blotting after chondroitinase ABC digestion (F) (SG, standard of serglycin used as positive control).

    Techniques Used: Fractionation, Multiple Displacement Amplification, SDS Page, Staining, Western Blot, Positive Control, GPC Assay

    6) Product Images from "A novel liposomal formulation of flavopiridol"

    Article Title: A novel liposomal formulation of flavopiridol

    Journal: International journal of pharmaceutics

    doi: 10.1016/j.ijpharm.2008.08.008

    In vitro release of flavopiridol from the HSPC/Chol/PEG-DSPE liposome. Flavopiridol-entrapped HSPC/Chol/PEG-DSPE liposome in PBS was incubated at 37 °C. At different time points, the liposomes were separated from the released free drug by Sepharose CL-4B column followed by the measurement of drug amount retained in liposome. Results are the mean of 3 separate experiments. Error bars stand for standard deviations, n = 3.
    Figure Legend Snippet: In vitro release of flavopiridol from the HSPC/Chol/PEG-DSPE liposome. Flavopiridol-entrapped HSPC/Chol/PEG-DSPE liposome in PBS was incubated at 37 °C. At different time points, the liposomes were separated from the released free drug by Sepharose CL-4B column followed by the measurement of drug amount retained in liposome. Results are the mean of 3 separate experiments. Error bars stand for standard deviations, n = 3.

    Techniques Used: In Vitro, Incubation

    Related Articles

    Centrifugation:

    Article Title: Streptococcus salivarius Fimbriae Are Composed of a Glycoprotein Containing a Repeated Motif Assembled into a Filamentous Nondissociable Structure
    Article Snippet: .. Insoluble material was removed by centrifugation (10,000 × g , 2 min), and the supernatant (1-ml samples) was applied to a Sepharose CL-4B column (1.5 by 25 cm; fractionation range, 60 × 103 to 20 × 106 Da; Sigma) at room temperature equilibrated with 20 mM Tris-HCl (pH 8.0)–0.2 M NaCl (buffer B) containing 0.1% SDS. ..

    Size-exclusion Chromatography:

    Article Title: Quantification of small extracellular vesicles by size exclusion chromatography with fluorescence detection
    Article Snippet: .. SEC columns of 10 mm × 10 cm packed with Sepharose CL-4B (Sigma-Aldrich, St. Louis, MO) were in-house prepared. ..

    Purification:

    Article Title: Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine
    Article Snippet: .. Purification of RHDV VLP on Sepharose CL4B For scale-up or purification of larger quantities of RHDV VLP, a XK26/35 column (350 mm×26 mm) packed with Sepharose CL4B (Sigma, USA) was used, equilibrated with phosphate buffer at the appropriate pH. ..

    Immunoprecipitation:

    Article Title: Cloning and Characterization of Mouse RIP140, a Corepressor for Nuclear Orphan Receptor TR2
    Article Snippet: .. For immunoprecipitation, 100 μl of the cell lysate was incubated with a mouse anti-HA monoclonal antibody (for HA-TR2) (Boehringer Mannheim, Indianapolis, Ind.) at 4°C for 2 h, and 15 μl of protein A-Sepharose CL4B resin (Sigma, St. Louis, Mo.) was added to the reaction for another 2 h of incubation. .. The resin was then washed three times with lysis buffer and resuspended in SDS-PAGE loading buffer for immunoblot analysis.

    Incubation:

    Article Title: MUC1 expression and anti-MUC1 serum immune response in head and neck squamous cell carcinoma (HNSCC): a multivariate analysis
    Article Snippet: .. Circulating immune complexes were resuspended in 1 ml PBS, applied to a 15 × 1 cm protein A sepharose CL4B column (Sigma, USA) and incubated for 30 min at 4°C. ..

    Article Title: Cloning and Characterization of Mouse RIP140, a Corepressor for Nuclear Orphan Receptor TR2
    Article Snippet: .. For immunoprecipitation, 100 μl of the cell lysate was incubated with a mouse anti-HA monoclonal antibody (for HA-TR2) (Boehringer Mannheim, Indianapolis, Ind.) at 4°C for 2 h, and 15 μl of protein A-Sepharose CL4B resin (Sigma, St. Louis, Mo.) was added to the reaction for another 2 h of incubation. .. The resin was then washed three times with lysis buffer and resuspended in SDS-PAGE loading buffer for immunoblot analysis.

    other:

    Article Title: The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay
    Article Snippet: Sepharose CL-4B and reagent solvents such as guanidinium chloride (GuHCl), phenylmethylsulfonylfluoride (PMSF), caesium chloride (CsCl), ethylenediaminetetra-acetic acid disodium salt (Na2 -EDTA), N-ethylmaleimide (NEM), and 3-((3-cholamidopropyl)-dimethyl-ammonio)-1-propane-sulfonate (CHAPS) were from Sigma (UK).

    Fractionation:

    Article Title: Streptococcus salivarius Fimbriae Are Composed of a Glycoprotein Containing a Repeated Motif Assembled into a Filamentous Nondissociable Structure
    Article Snippet: .. Insoluble material was removed by centrifugation (10,000 × g , 2 min), and the supernatant (1-ml samples) was applied to a Sepharose CL-4B column (1.5 by 25 cm; fractionation range, 60 × 103 to 20 × 106 Da; Sigma) at room temperature equilibrated with 20 mM Tris-HCl (pH 8.0)–0.2 M NaCl (buffer B) containing 0.1% SDS. ..

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