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Bio-Rad sepharose 4b immunoaffinity columns
Sepharose 4b Immunoaffinity Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sepharose 4b immunoaffinity columns/product/Bio-Rad
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sepharose 4b immunoaffinity columns - by Bioz Stars, 2021-03
86/100 stars

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Purification:

Article Title: Loss of HDAC11 ameliorates clinical symptoms in a multiple sclerosis mouse model
Article Snippet: To generate an anti-HDAC11 antibody, a peptide corresponding to amino acids 179–193 of HDAC11 (DLDAHQGNGHERDFM) coupled to keyhole limpet hemocyanin was injected subcutaneously into New Zealand white rabbits. .. The resulting antibody was immunoaffinity purified on a peptide column with Affi-gel agarose (Bio-Rad). .. Specificity of this anti-HDAC11 antibody was confirmed using brain tissue extracts from HDAC11 KO and WT mice.

Article Title: ?F508 CFTR protein expression in tissues from patients with cystic fibrosis
Article Snippet: The protein was purified from inclusion bodies, solubilized in 8 M urea and 2% SDS, and isolated prior to immunization from preparative polyacrylamide gels ( ). .. Both antisera were purified by immunoaffinity chromatography with the CFTR antigen, which was coupled to activated Affi-Gel 10 agarose columns (Bio-Rad Laboratories Inc., Hercules, California, USA). .. After washing the columns with 5 volumes 0.5 M NaCl, antibodies were eluted with 4.9 M MgCl2 for the peptide and 50 mM diethylamine (pH 11.5) for the recombinant CFTR antigen.

Incubation:

Article Title: Apolipoproteins E and CIII interact to regulate HDL metabolism and coronary heart disease risk
Article Snippet: .. Briefly, thawed plasma from each time point was incubated with Sepharose 4B immunoaffinity columns (Bio-Rad) containing affinity purified polyclonal antibodies to apoA-I (catalog 11A-G2b, Academy Bio-Medical Co.). ..

Article Title: Dietary unsaturated fat increases HDL metabolic pathways involving apoE favorable to reverse cholesterol transport
Article Snippet: .. Briefly, thawed plasma from each time point was incubated with Sepharose 4B immunoaffinity columns (Bio-Rad) containing affinity-purified polyclonal antibodies against apoA-I (Academy Biomedical, catalog 11A-G2b). ..

Affinity Purification:

Article Title: Apolipoproteins E and CIII interact to regulate HDL metabolism and coronary heart disease risk
Article Snippet: .. Briefly, thawed plasma from each time point was incubated with Sepharose 4B immunoaffinity columns (Bio-Rad) containing affinity purified polyclonal antibodies to apoA-I (catalog 11A-G2b, Academy Bio-Medical Co.). ..

Article Title: Dietary unsaturated fat increases HDL metabolic pathways involving apoE favorable to reverse cholesterol transport
Article Snippet: .. Briefly, thawed plasma from each time point was incubated with Sepharose 4B immunoaffinity columns (Bio-Rad) containing affinity-purified polyclonal antibodies against apoA-I (Academy Biomedical, catalog 11A-G2b). ..

Chromatography:

Article Title: ?F508 CFTR protein expression in tissues from patients with cystic fibrosis
Article Snippet: The protein was purified from inclusion bodies, solubilized in 8 M urea and 2% SDS, and isolated prior to immunization from preparative polyacrylamide gels ( ). .. Both antisera were purified by immunoaffinity chromatography with the CFTR antigen, which was coupled to activated Affi-Gel 10 agarose columns (Bio-Rad Laboratories Inc., Hercules, California, USA). .. After washing the columns with 5 volumes 0.5 M NaCl, antibodies were eluted with 4.9 M MgCl2 for the peptide and 50 mM diethylamine (pH 11.5) for the recombinant CFTR antigen.

Liquid Chromatography:

Article Title: A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine
Article Snippet: Immunoaffinity chromatography IgGs from all three sample groups were covalently bound to CNBr-activated Sepharose (GE Healthcare, UK) according to the manufacturer’s protocol. .. Sepharose with bound immunoglobulins was loaded into 3 plastic cartridges (BioRad, USA) creating liquid chromatography columns. .. The same antigen (protein lysate from Salmonella Typhimurium grown in LB) was used for all three IgG columns - column with antibodies from negative control animals, from vaccinated animals and from infected animals.

Flow Cytometry:

Article Title: Rapid Isolation of a Single-Chain Antibody against the Cyanobacterial Toxin Microcystin-LR by Phage Display and Its Use in the Immunoaffinity Concentration of Microcystins from Water
Article Snippet: The microcystin concentrations in each extract were then determined in duplicate by indirect competition ELISA. .. The anti-microcystin-LR immunoaffinity gel was prepared by coupling 30 μg of 3A8 scAb to 0.5 ml of Ni2+ charged Fast Flow Sepharose for 2 h at room temperature with gentle rotation in a polypropylene column (Bio-Rad, Hercules, Calif.). ..

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    Bio-Rad sepharose 4b immunoaffinity columns
    Sepharose 4b Immunoaffinity Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sepharose 4b immunoaffinity columns/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sepharose 4b immunoaffinity columns - by Bioz Stars, 2021-03
    86/100 stars
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