semithin sections  (Millipore)


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    Structured Review

    Millipore semithin sections
    Bacterial communities associated with A. aurita polyps identified by FISH analysis. Shown are epifluorescence micrographs of bacteria on <t>semithin</t> sections (0.5 μm) of untreated (left) and antibiotic-treated (right) A. aurita polyps (Roscoff).
    Semithin Sections, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semithin sections/product/Millipore
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    semithin sections - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population"

    Article Title: Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01601-15

    Bacterial communities associated with A. aurita polyps identified by FISH analysis. Shown are epifluorescence micrographs of bacteria on semithin sections (0.5 μm) of untreated (left) and antibiotic-treated (right) A. aurita polyps (Roscoff).
    Figure Legend Snippet: Bacterial communities associated with A. aurita polyps identified by FISH analysis. Shown are epifluorescence micrographs of bacteria on semithin sections (0.5 μm) of untreated (left) and antibiotic-treated (right) A. aurita polyps (Roscoff).

    Techniques Used: Fluorescence In Situ Hybridization

    2) Product Images from "Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats"

    Article Title: Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.171266298

    Anatomical rescue of photoreceptors after transplantation of h1RPE7 in RCS rats. Semithin sections of retinae taken from animals used in the electrophysiological studies are shown. ( A ) A 6-month-old nondystrophic RCS rat showing full ONL thickness. ( B ) h1RPE7-transplanted RCS rat 5 months postgraft demonstrating significant preservation of the ONL; this level of rescue corresponds to a threshold sensitivity 2 log units above background. Area shown was 1,400 μm from injection site. ( C ) Sham-operated RCS rat 5 months postoperative showing complete loss of the ONL; there is also marked disruption of the INL. All sections were stained with toluidine blue. GC, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; IS, inner segments; OS, outer segments. [Bar = 20 μm.]
    Figure Legend Snippet: Anatomical rescue of photoreceptors after transplantation of h1RPE7 in RCS rats. Semithin sections of retinae taken from animals used in the electrophysiological studies are shown. ( A ) A 6-month-old nondystrophic RCS rat showing full ONL thickness. ( B ) h1RPE7-transplanted RCS rat 5 months postgraft demonstrating significant preservation of the ONL; this level of rescue corresponds to a threshold sensitivity 2 log units above background. Area shown was 1,400 μm from injection site. ( C ) Sham-operated RCS rat 5 months postoperative showing complete loss of the ONL; there is also marked disruption of the INL. All sections were stained with toluidine blue. GC, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; IS, inner segments; OS, outer segments. [Bar = 20 μm.]

    Techniques Used: Transplantation Assay, Preserving, Injection, Staining

    3) Product Images from "Characterization of diabetic neuropathy progression in a mouse model of type 2 diabetes mellitus"

    Article Title: Characterization of diabetic neuropathy progression in a mouse model of type 2 diabetes mellitus

    Journal: Biology Open

    doi: 10.1242/bio.036830

    Fiber changes analysis in sciatic nerves of db/db mice. (A) Representative bright-field images of semithin sections of sciatic nerves derived from diabetic and non-diabetic mice (18, 26 and 32 weeks of age). Myelinated fibers were stained with Toluidine Blue. (B) Quantification of diameter of nerve fibers in sciatic nerve. Data are presented as mean diameter±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). (C) Quantification of fiber density in the sciatic nerve. Data are presented as mean fiber number per area±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). Scale bar: 50 µm. * P
    Figure Legend Snippet: Fiber changes analysis in sciatic nerves of db/db mice. (A) Representative bright-field images of semithin sections of sciatic nerves derived from diabetic and non-diabetic mice (18, 26 and 32 weeks of age). Myelinated fibers were stained with Toluidine Blue. (B) Quantification of diameter of nerve fibers in sciatic nerve. Data are presented as mean diameter±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). (C) Quantification of fiber density in the sciatic nerve. Data are presented as mean fiber number per area±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). Scale bar: 50 µm. * P

    Techniques Used: Mouse Assay, Derivative Assay, Staining

    4) Product Images from "Murine Muscle Engineered from Dermal Precursors: An In Vitro Model for Skeletal Muscle Generation, Degeneration, and Fatty Infiltration"

    Article Title: Murine Muscle Engineered from Dermal Precursors: An In Vitro Model for Skeletal Muscle Generation, Degeneration, and Fatty Infiltration

    Journal: Tissue Engineering. Part C, Methods

    doi: 10.1089/ten.tec.2013.0146

    Ultrastructural characterization of myotubes. (A–C) . Aspect of semithin (1.5-μm) sections stained with Toluidine blue. (A) Semithin sections showing tubular and spherical cells (scale bar, 100 μm). (B) Longitudinally oriented
    Figure Legend Snippet: Ultrastructural characterization of myotubes. (A–C) . Aspect of semithin (1.5-μm) sections stained with Toluidine blue. (A) Semithin sections showing tubular and spherical cells (scale bar, 100 μm). (B) Longitudinally oriented

    Techniques Used: Staining

    5) Product Images from "Characterization of diabetic neuropathy progression in a mouse model of type 2 diabetes mellitus"

    Article Title: Characterization of diabetic neuropathy progression in a mouse model of type 2 diabetes mellitus

    Journal: Biology Open

    doi: 10.1242/bio.036830

    Fiber changes analysis in sciatic nerves of db/db mice. (A) Representative bright-field images of semithin sections of sciatic nerves derived from diabetic and non-diabetic mice (18, 26 and 32 weeks of age). Myelinated fibers were stained with Toluidine Blue. (B) Quantification of diameter of nerve fibers in sciatic nerve. Data are presented as mean diameter±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). (C) Quantification of fiber density in the sciatic nerve. Data are presented as mean fiber number per area±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). Scale bar: 50 µm. * P
    Figure Legend Snippet: Fiber changes analysis in sciatic nerves of db/db mice. (A) Representative bright-field images of semithin sections of sciatic nerves derived from diabetic and non-diabetic mice (18, 26 and 32 weeks of age). Myelinated fibers were stained with Toluidine Blue. (B) Quantification of diameter of nerve fibers in sciatic nerve. Data are presented as mean diameter±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). (C) Quantification of fiber density in the sciatic nerve. Data are presented as mean fiber number per area±s.e.m. ( n =6, two-way ANOVA with Bonferroni post-test). Scale bar: 50 µm. * P

    Techniques Used: Mouse Assay, Derivative Assay, Staining

    6) Product Images from "MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development 1MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development 1 [OPEN]"

    Article Title: MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development 1MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development 1 [OPEN]

    Journal: Plant Physiology

    doi: 10.1104/pp.16.00988

    MON1 mutation results in delayed tapetum degeneration. Semithin sections showed delayed tapetum degeneration in mon1-2 compared with the wild type (WT). Transverse semithin sections of wild-type (A–F) and mon1-2 (G–L) anthers at indicated
    Figure Legend Snippet: MON1 mutation results in delayed tapetum degeneration. Semithin sections showed delayed tapetum degeneration in mon1-2 compared with the wild type (WT). Transverse semithin sections of wild-type (A–F) and mon1-2 (G–L) anthers at indicated

    Techniques Used: Mutagenesis

    7) Product Images from "Application of q-Space Diffusion MRI for the Visualization of White Matter"

    Article Title: Application of q-Space Diffusion MRI for the Visualization of White Matter

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1770-15.2016

    Myelin map of common marmosets with chemically induced focal demyelination. Axial images of spinal cord obtained 1 week after chemically induced demyelination, showing T2WI and myelin map results in a live animal, and postmortem LFB, semithin sections, and electron microscopic (EM) analysis ( A ). The same set of axial images was obtained 6 weeks after demyelination ( B ). The g -ratio, calculated by dividing axonal diameter by myelinated fiber diameter (e.g., the g -ratio for a completely demyelinated axon is 1), shows decreased values at 6 weeks after injury (0.74 ± 0.070; mean ± SE) compared with 1 week postinjury (0.90 ± 0.095), suggestive of remyelination ( C ). Quantitative comparative analysis of demyelinated areas in the dorsal funiculus using a myelin map in a live animal and postmortem LFB ( n = 4 each for 1 and 6 weeks after injury) suggests the high accuracy of the myelin map in detecting demyelinated areas ( D ). Note that T2WI failed to detect demyelinating lesions clearly in this model. a.u., Arbitrary units. Scale bars: LFB, 1 μm; EM, 10 μm. Error bars indicate SEM.
    Figure Legend Snippet: Myelin map of common marmosets with chemically induced focal demyelination. Axial images of spinal cord obtained 1 week after chemically induced demyelination, showing T2WI and myelin map results in a live animal, and postmortem LFB, semithin sections, and electron microscopic (EM) analysis ( A ). The same set of axial images was obtained 6 weeks after demyelination ( B ). The g -ratio, calculated by dividing axonal diameter by myelinated fiber diameter (e.g., the g -ratio for a completely demyelinated axon is 1), shows decreased values at 6 weeks after injury (0.74 ± 0.070; mean ± SE) compared with 1 week postinjury (0.90 ± 0.095), suggestive of remyelination ( C ). Quantitative comparative analysis of demyelinated areas in the dorsal funiculus using a myelin map in a live animal and postmortem LFB ( n = 4 each for 1 and 6 weeks after injury) suggests the high accuracy of the myelin map in detecting demyelinated areas ( D ). Note that T2WI failed to detect demyelinating lesions clearly in this model. a.u., Arbitrary units. Scale bars: LFB, 1 μm; EM, 10 μm. Error bars indicate SEM.

    Techniques Used:

    8) Product Images from "Galectin-3 Is a Downstream Regulator of Matrix Metalloproteinase-9 Function during Endochondral Bone Formation"

    Article Title: Galectin-3 Is a Downstream Regulator of Matrix Metalloproteinase-9 Function during Endochondral Bone Formation

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E04-12-1119

    Altered organization of the chondro-osseous junction in Mmp-9 null mice. Histological analysis of the chondro-osseous junction. (a and b) Representative pictures of semithin sections stained with Toluidine blue showing the abnormal survival of the last row of hypertrophic chondrocyte (arrows) in Mmp-9 null mice compared with WT. A capillary in close contact with last transverse septa is marked by an asterisk ( * ). (c–h) Longitudinal sections through the central part of growth plates. (c and d) Alcian blue staining; note the decrease in staining in Mmp-9 null mice in the last row of hypertrophic chondrocytes and trabeculae. (e and f) PECAM staining showing no major differences in growth plate vascularization between Mmp-9 null and WT mice. (g and h) TRAP staining, note the increase in TRAP-positive cells recruited at the chondro-osseous junction in Mmp-9 null mice. hc, hypertrophic chondrocytes; tb, trabecular bone. Scale bars, 125 μm. (i) Quantification of mononuclear and multinucleated TRAP-positive cells recruited at the chondro-osseous junction, showing an increase in Mmp-9 null mice (n = 6 different metatarsals; * p ≤ 0.005).
    Figure Legend Snippet: Altered organization of the chondro-osseous junction in Mmp-9 null mice. Histological analysis of the chondro-osseous junction. (a and b) Representative pictures of semithin sections stained with Toluidine blue showing the abnormal survival of the last row of hypertrophic chondrocyte (arrows) in Mmp-9 null mice compared with WT. A capillary in close contact with last transverse septa is marked by an asterisk ( * ). (c–h) Longitudinal sections through the central part of growth plates. (c and d) Alcian blue staining; note the decrease in staining in Mmp-9 null mice in the last row of hypertrophic chondrocytes and trabeculae. (e and f) PECAM staining showing no major differences in growth plate vascularization between Mmp-9 null and WT mice. (g and h) TRAP staining, note the increase in TRAP-positive cells recruited at the chondro-osseous junction in Mmp-9 null mice. hc, hypertrophic chondrocytes; tb, trabecular bone. Scale bars, 125 μm. (i) Quantification of mononuclear and multinucleated TRAP-positive cells recruited at the chondro-osseous junction, showing an increase in Mmp-9 null mice (n = 6 different metatarsals; * p ≤ 0.005).

    Techniques Used: Mouse Assay, Staining

    9) Product Images from "Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56 ▿"

    Article Title: Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56 ▿

    Journal: Eukaryotic Cell

    doi: 10.1128/EC.00292-07

    Functional characterization of antigens recognized by E2E5. (A and B) Semithin sections of ceca from infected chickens. (A) Macrogamonts were labeled with E2E5 and an anti-mouse IgG antibody coupled to FITC. (B) The same parasites are shown by bright-field microscopy. (C) Localization of E2E5-reactive antigens at the inner oocyst wall (black arrowhead) is shown by double labeling with E2E5 and anti-mouse IgG-FITC and then with E1D8 and anti-mouse IgM-rhodamine. E1D8 recognizes the outer oocyst wall (white arrowhead). (D) Localization of E2E5-binding structures in sporocysts. Oocysts were mechanically ruptured with glass beads before being labeled with E2E5 and a goat anti-mouse Alexa 488-coupled secondary antibody. The arrow indicates the position of the stieda body. The arrowhead marks a small rupture of the wall in the sporocyst. Separate phase-contrast and green fluorescence pictures were taken and merged using Corel Photo-Paint 10. Bars, 10 μm. (E) Inhibition of in vitro excystation by the monoclonal antibody E2E5. In vitro excystation was performed in the presence of 50-fold-concentrated E2E5 or control supernatant derived from the hybridoma E1D8 or from Jurkat cells. As an isotype control, concentrated Jurkat cell supernatant was supplemented with 250 μg/ml anti-V5 IgG2a antibody (Invitrogen). Excystation efficiencies, evaluated as the number of released sporozoites per total number of sporozoites contained in sporocysts, were calculated and normalized to the mean excystation efficiency of the control. *, P
    Figure Legend Snippet: Functional characterization of antigens recognized by E2E5. (A and B) Semithin sections of ceca from infected chickens. (A) Macrogamonts were labeled with E2E5 and an anti-mouse IgG antibody coupled to FITC. (B) The same parasites are shown by bright-field microscopy. (C) Localization of E2E5-reactive antigens at the inner oocyst wall (black arrowhead) is shown by double labeling with E2E5 and anti-mouse IgG-FITC and then with E1D8 and anti-mouse IgM-rhodamine. E1D8 recognizes the outer oocyst wall (white arrowhead). (D) Localization of E2E5-binding structures in sporocysts. Oocysts were mechanically ruptured with glass beads before being labeled with E2E5 and a goat anti-mouse Alexa 488-coupled secondary antibody. The arrow indicates the position of the stieda body. The arrowhead marks a small rupture of the wall in the sporocyst. Separate phase-contrast and green fluorescence pictures were taken and merged using Corel Photo-Paint 10. Bars, 10 μm. (E) Inhibition of in vitro excystation by the monoclonal antibody E2E5. In vitro excystation was performed in the presence of 50-fold-concentrated E2E5 or control supernatant derived from the hybridoma E1D8 or from Jurkat cells. As an isotype control, concentrated Jurkat cell supernatant was supplemented with 250 μg/ml anti-V5 IgG2a antibody (Invitrogen). Excystation efficiencies, evaluated as the number of released sporozoites per total number of sporozoites contained in sporocysts, were calculated and normalized to the mean excystation efficiency of the control. *, P

    Techniques Used: Functional Assay, Infection, Labeling, Microscopy, Binding Assay, Fluorescence, Inhibition, In Vitro, Derivative Assay

    Related Articles

    In Situ:

    Article Title: Generating a Knockdown Transgene against Drosophila Heterochromatic Tim17b Gene Encoding Mitochondrial Translocase Subunit
    Article Snippet: .. Apoptosis detection We used the ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Millipore # S7110) to detect the occurrence of cell death in first instar larvae brains. .. Tissues from wild type or larvae expressing Tim17bRNAi were dissected in Grace's, fixed as described above, and washed 10 min in PBT.

    CtB Assay:

    Article Title: PTEN knockdown with the Y444F mutant AAV2 vector promotes axonal regeneration in the adult optic nerve
    Article Snippet: .. AAV2 vectors (5 μL, titers at 1.0 × 1012 ) or cholera toxin β subunit-conjugated fluorescein isothiocyanate C (CTB-FITC) (5 μL of 0.2%; Sigma, St. Louis, MO, USA) were intravitreally injected using a 5-μL Hamilton syringe (Hamilton, Bonaduz, Switzerland) (Koch et al., 2014). ..

    Mouse Assay:

    Article Title: Topical 1,25-dihydroxyvitamin D3 subverts the priming ability of draining lymph node dendritic cells
    Article Snippet: .. The fluorescent antigen, 0·5% fluorescein isothiocyanate (FITC; Sigma, diluted 1 : 1 in acetone : dibutylphthalate) was painted onto the skin of mice 30 min after topical treatment. ..

    Invadopodia Assay:

    Article Title: The ZEB1/miR-200c feedback loop regulates invasion via actin interacting proteins MYLK and TKS5
    Article Snippet: .. Gelatin degradation assay Fluorescein-gelatin coating of coverslips was performed using the QCM Gelatin Invadopodia assay kit (Millipore) according to manufacturer's instructions. .. Cells were seeded on fluorescein-gelatin coated coverslips 24 h post-transfection and incubated for another 24 h. Following fixation with 4% paraformaldehyde (PFA), F-actin was labeled using rhodamine-phalloidin (Life technologies; 1:50) and nuclei were stained with Hoechst dye (Molecular Probes).

    Concentration Assay:

    Article Title: d-Alanine Modification of a Protease-Susceptible Outer Membrane Component by the Bordetella pertussisdra Locus Promotes Resistance to Antimicrobial Peptides and Polymorphonuclear Leukocyte-Mediated Killing
    Article Snippet: .. Cytochrome c (Sigma) was added to a final concentration of 500 μg/ml, whereas fluorescein-labeled LL-37 and fluorescein isothiocyanate (FITC)-labeled poly- l -lysine (Sigma) were added at a concentration of 20 μg/ml. .. After incubation for 10 min at room temperature, bacteria were centrifuged at 13,000 rpm for 5 min, washed twice with PBS, and then resuspended in PBS.

    Incubation:

    Article Title: Trichoderma asperellum T42 Reprograms Tobacco for Enhanced Nitrogen Utilization Efficiency and Plant Growth When Fed with N Nutrients
    Article Snippet: .. The samples were then incubated in 10 μM Fluo-4 AM [4-(6-Acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′, N′-tetraacetic acid tetra -kis (acetoxymethyl) ester; Sigma–Aldrich] in loading buffer [diluted from 5 mM stock solution in 10 mM MES-KOH; 2-(N-Morpholino) ethanesulfonic acid sodium salt, 4-Morpholineethanesulfonic acid sodium salt; pH 6.15] for 2 h at 4°C in darkness. .. Root samples were rinsed thrice with loading buffer to remove excess dye and kept at 24°C in the growth chamber for 1–2 h. Fluorescence intensity was increased when intracellular esterase removed AM from Fluo-4AM and bind to cytosolic Ca2+ , measured at excitation (488 nm) and emission (510–545 nm) wavelength.

    Article Title: Ezrin Is an Effector of Hepatocyte Growth Factor-mediated Migration and Morphogenesis in Epithelial Cells
    Article Snippet: .. Transwell filters were then incubated with rhodamine-coupled anti–rabbit, and fluorescein-linked anti–mouse IgG antibodies, mounted on glass slides in a solution of Mowiol ( Calbiochem-Novabiochem Corp. , La Jolla, CA) and viewed on a Leica CLSM (Vienna, Austria). .. For each X-Y section, the intensity of fluorescence was determined with arbitrary units 1–255.

    Confocal Laser Scanning Microscopy:

    Article Title: Ezrin Is an Effector of Hepatocyte Growth Factor-mediated Migration and Morphogenesis in Epithelial Cells
    Article Snippet: .. Transwell filters were then incubated with rhodamine-coupled anti–rabbit, and fluorescein-linked anti–mouse IgG antibodies, mounted on glass slides in a solution of Mowiol ( Calbiochem-Novabiochem Corp. , La Jolla, CA) and viewed on a Leica CLSM (Vienna, Austria). .. For each X-Y section, the intensity of fluorescence was determined with arbitrary units 1–255.

    Degradation Assay:

    Article Title: The ZEB1/miR-200c feedback loop regulates invasion via actin interacting proteins MYLK and TKS5
    Article Snippet: .. Gelatin degradation assay Fluorescein-gelatin coating of coverslips was performed using the QCM Gelatin Invadopodia assay kit (Millipore) according to manufacturer's instructions. .. Cells were seeded on fluorescein-gelatin coated coverslips 24 h post-transfection and incubated for another 24 h. Following fixation with 4% paraformaldehyde (PFA), F-actin was labeled using rhodamine-phalloidin (Life technologies; 1:50) and nuclei were stained with Hoechst dye (Molecular Probes).

    Injection:

    Article Title: PTEN knockdown with the Y444F mutant AAV2 vector promotes axonal regeneration in the adult optic nerve
    Article Snippet: .. AAV2 vectors (5 μL, titers at 1.0 × 1012 ) or cholera toxin β subunit-conjugated fluorescein isothiocyanate C (CTB-FITC) (5 μL of 0.2%; Sigma, St. Louis, MO, USA) were intravitreally injected using a 5-μL Hamilton syringe (Hamilton, Bonaduz, Switzerland) (Koch et al., 2014). ..

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    Millipore semithin sections
    Semithin Sections, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semithin sections/product/Millipore
    Average 93 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    semithin sections - by Bioz Stars, 2020-08
    93/100 stars
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