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Agilent technologies semi preparative hplc
In-frame deletion of pya25 and pya26 in S. netropsis . <t>HPLC</t> analysis of <t>pyrrolamides</t> production in S. netropsis wild-type strain, the mutant strains WDY002 (Δ pya25 ) and WDY003 (Δ pya26 ), and the complementation strains WDY004 (negative control) and WDY005. Congocidine, Compound 3 , and Distamycin are indicated. The characteristic absorbance wave-length for pyrrolamides is 297 nm.
Semi Preparative Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Mining of the Pyrrolamide Antibiotics Analogs in Streptomyces netropsis Reveals the Amidohydrolase-Dependent "Iterative Strategy" Underlying the Pyrrole Polymerization"

Article Title: Mining of the Pyrrolamide Antibiotics Analogs in Streptomyces netropsis Reveals the Amidohydrolase-Dependent "Iterative Strategy" Underlying the Pyrrole Polymerization

Journal: PLoS ONE

doi: 10.1371/journal.pone.0099077

In-frame deletion of pya25 and pya26 in S. netropsis . HPLC analysis of pyrrolamides production in S. netropsis wild-type strain, the mutant strains WDY002 (Δ pya25 ) and WDY003 (Δ pya26 ), and the complementation strains WDY004 (negative control) and WDY005. Congocidine, Compound 3 , and Distamycin are indicated. The characteristic absorbance wave-length for pyrrolamides is 297 nm.
Figure Legend Snippet: In-frame deletion of pya25 and pya26 in S. netropsis . HPLC analysis of pyrrolamides production in S. netropsis wild-type strain, the mutant strains WDY002 (Δ pya25 ) and WDY003 (Δ pya26 ), and the complementation strains WDY004 (negative control) and WDY005. Congocidine, Compound 3 , and Distamycin are indicated. The characteristic absorbance wave-length for pyrrolamides is 297 nm.

Techniques Used: High Performance Liquid Chromatography, Mutagenesis, Negative Control

2) Product Images from "Bis-butanediol-mercapturic acid (bis-BDMA) as a urinary biomarker of metabolic activation of butadiene to its ultimate carcinogenic species"

Article Title: Bis-butanediol-mercapturic acid (bis-BDMA) as a urinary biomarker of metabolic activation of butadiene to its ultimate carcinogenic species

Journal: Carcinogenesis

doi: 10.1093/carcin/bgu047

Full scan accurate mass ESI − -QTOF spectrum of bis -BDMA and 2 H 6 - bis -BDMA obtained on a Waters Acquity UPLC/Synapt G2 QTOF mass spectrometer ( A ) and HPLC-ESI − -MS/MS detection of bis -BDMA in urine of F344 rats exposed to 0 p.p.m. of BD and
Figure Legend Snippet: Full scan accurate mass ESI − -QTOF spectrum of bis -BDMA and 2 H 6 - bis -BDMA obtained on a Waters Acquity UPLC/Synapt G2 QTOF mass spectrometer ( A ) and HPLC-ESI − -MS/MS detection of bis -BDMA in urine of F344 rats exposed to 0 p.p.m. of BD and

Techniques Used: Mass Spectrometry, High Performance Liquid Chromatography

3) Product Images from "Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties"

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2017.00221

Production of polyketomycin and foxicin in Streptomyces diastatochromogenes Tü6028. (A) HPLC chromatogram of S. diastatochromogenes Tü6028 wild type at λ430 nm with polyketomycin structure (top) and of Δ pokOIV mutant at λ320 nm (below); (B) Production of foxicin A (▴) and polyketomycin (O) in S. diastatochromogenes Tü6028 wild type; (C) Morphology of S. diastatochromogenes Tü6028 wild type (left) and Δ pokOIV mutant (right). On the plate, the Δ pokOIV mutant appears yellow and deficient in producing spores and melanins (dark color).
Figure Legend Snippet: Production of polyketomycin and foxicin in Streptomyces diastatochromogenes Tü6028. (A) HPLC chromatogram of S. diastatochromogenes Tü6028 wild type at λ430 nm with polyketomycin structure (top) and of Δ pokOIV mutant at λ320 nm (below); (B) Production of foxicin A (▴) and polyketomycin (O) in S. diastatochromogenes Tü6028 wild type; (C) Morphology of S. diastatochromogenes Tü6028 wild type (left) and Δ pokOIV mutant (right). On the plate, the Δ pokOIV mutant appears yellow and deficient in producing spores and melanins (dark color).

Techniques Used: High Performance Liquid Chromatography, Mutagenesis

4) Product Images from "Multi-arm Nanoconjugates for Cancer Cell-Targeted Delivery of Photosensitizers"

Article Title: Multi-arm Nanoconjugates for Cancer Cell-Targeted Delivery of Photosensitizers

Journal: Molecular pharmaceutics

doi: 10.1021/acs.molpharmaceut.8b00088

Physicochemical characterization of nanoconjugates. A. SEC-HPLC chromatography of IR700, 8PEG-IR700 conjugates, and RGD-8PEG-IR700 nanoconjugates (λ = 689 nm), as well as BSA and IgG (λ = 280 nm). B. Particle size distribution of RGD-8PEG-IR700 nanoconjugates determined with DLS. C. Singlet oxygen generation of IR700, 8PEG-IR700, and RGD-8PEG-IR700.
Figure Legend Snippet: Physicochemical characterization of nanoconjugates. A. SEC-HPLC chromatography of IR700, 8PEG-IR700 conjugates, and RGD-8PEG-IR700 nanoconjugates (λ = 689 nm), as well as BSA and IgG (λ = 280 nm). B. Particle size distribution of RGD-8PEG-IR700 nanoconjugates determined with DLS. C. Singlet oxygen generation of IR700, 8PEG-IR700, and RGD-8PEG-IR700.

Techniques Used: Size-exclusion Chromatography, High Performance Liquid Chromatography, Chromatography

5) Product Images from "Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies"

Article Title: Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies

Journal: Microbial Cell Factories

doi: 10.1186/s12934-020-01418-w

a The CRM A production titer of mutant XC-11GUR on Medium N2 (N2) and optimized medium N2 (ON2). b The HPLC analysis of CRM A production (asterisk) of mutant XC-11GUR on Medium N2 (i) and Optimized Medium N2 (ii)
Figure Legend Snippet: a The CRM A production titer of mutant XC-11GUR on Medium N2 (N2) and optimized medium N2 (ON2). b The HPLC analysis of CRM A production (asterisk) of mutant XC-11GUR on Medium N2 (i) and Optimized Medium N2 (ii)

Techniques Used: Mutagenesis, High Performance Liquid Chromatography

a Phylogenetic analysis of Actinoalloteichus sp. AHMU CJ021. Numbers at the nodes represent bootstrap percentages obtained from 1000 replicates. The scale bar (0.005) represents nucleotide substitutions per site. Sequence alignment was performed with ClustalW. A phylogenetic tree was constructed with MEGA 7.0 software using neighbor-joining algorithm based on 16S rDNA gene sequences; b HPLC analyses of CRM A production (asterisk) in Actinoalloteichus sp. AHMU CJ021 wild type and mutant strains. (i) Actinoalloteichus sp. AHMU CJ021 wild type; (ii–iv) XC-11G, XC-11GU and XC-11GUR respectively; Transcriptional analysis, c RT-PCR assay and d qPCR assay, determined camE expression in the Actinoalloteichus sp. AHMU CJ021wild type strain ( WT ) and three mutants: XC-16R ( M1 ), XC-14G ( M2 ) and XC-11G ( M3 ); e The bioassay comparison and screening of mutants generated by UV mutagenesis, the asterisk indicates the inhibition zone level of the original mutant XC-11G; the number of mutants belonging to each range is indicated up the column; f The cultured morphology of the Actinoalloteichus sp. AHMU CJ021 wild type strain and three mutants on M-ISP2 medium; g HPLC analyses for riboflavin (black solid circle) biosynthesis in the Actinoalloteichus sp. AHMU CJ021 wild type and mutant strains. (i) authentic standard of riboflavin; (ii) Actinoalloteichus sp. AHMU CJ021 wild type; (iii–v) XC-11G, XC-11GU and XC-11GUR, respectively
Figure Legend Snippet: a Phylogenetic analysis of Actinoalloteichus sp. AHMU CJ021. Numbers at the nodes represent bootstrap percentages obtained from 1000 replicates. The scale bar (0.005) represents nucleotide substitutions per site. Sequence alignment was performed with ClustalW. A phylogenetic tree was constructed with MEGA 7.0 software using neighbor-joining algorithm based on 16S rDNA gene sequences; b HPLC analyses of CRM A production (asterisk) in Actinoalloteichus sp. AHMU CJ021 wild type and mutant strains. (i) Actinoalloteichus sp. AHMU CJ021 wild type; (ii–iv) XC-11G, XC-11GU and XC-11GUR respectively; Transcriptional analysis, c RT-PCR assay and d qPCR assay, determined camE expression in the Actinoalloteichus sp. AHMU CJ021wild type strain ( WT ) and three mutants: XC-16R ( M1 ), XC-14G ( M2 ) and XC-11G ( M3 ); e The bioassay comparison and screening of mutants generated by UV mutagenesis, the asterisk indicates the inhibition zone level of the original mutant XC-11G; the number of mutants belonging to each range is indicated up the column; f The cultured morphology of the Actinoalloteichus sp. AHMU CJ021 wild type strain and three mutants on M-ISP2 medium; g HPLC analyses for riboflavin (black solid circle) biosynthesis in the Actinoalloteichus sp. AHMU CJ021 wild type and mutant strains. (i) authentic standard of riboflavin; (ii) Actinoalloteichus sp. AHMU CJ021 wild type; (iii–v) XC-11G, XC-11GU and XC-11GUR, respectively

Techniques Used: Sequencing, Construct, Software, High Performance Liquid Chromatography, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Expressing, Generated, Inhibition, Cell Culture

6) Product Images from "Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study"

Article Title: Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms19030793

3D structure, purification and identification of cyclotides. ( a ) 3D cartoon structure of kalata b1 (PDB ID:1KAL) illustrating the knotted pattern with three internal disulfide bonds (yellow and stick); ( b ) Semi-preparative HPLC showing elution of cyclotides from V. philippica , P1, P2, and P3; ( c ) A survey mass spectrum of four digested peptide fragments of Varv A; ( d ) Product ion spectrum of fragment 4 (derived from Varv A).
Figure Legend Snippet: 3D structure, purification and identification of cyclotides. ( a ) 3D cartoon structure of kalata b1 (PDB ID:1KAL) illustrating the knotted pattern with three internal disulfide bonds (yellow and stick); ( b ) Semi-preparative HPLC showing elution of cyclotides from V. philippica , P1, P2, and P3; ( c ) A survey mass spectrum of four digested peptide fragments of Varv A; ( d ) Product ion spectrum of fragment 4 (derived from Varv A).

Techniques Used: Purification, High Performance Liquid Chromatography, Derivative Assay

7) Product Images from "Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination"

Article Title: Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination

Journal: Journal of labelled compounds & radiopharmaceuticals

doi: 10.1002/jlcr.3487

HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.
Figure Legend Snippet: HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.

Techniques Used: High Performance Liquid Chromatography, Purification, Injection, Flow Cytometry

Related Articles

High Performance Liquid Chromatography:

Article Title: Mining of the Pyrrolamide Antibiotics Analogs in Streptomyces netropsis Reveals the Amidohydrolase-Dependent "Iterative Strategy" Underlying the Pyrrole Polymerization
Article Snippet: .. Purification of pyrrolamides was performed by semi-preparative HPLC of the crude extract on a Agilent ZORBAX SB-C18 column (5 µm, 9.4×250 mm) with a flow rate of 3 mL/min over a 35 min gradient (T = 0 min, 10% B; T = 20 min, 40% B; T = 25 min, 100% B; T = 35 min, 100%; solvent A, water; solvent B, acetonitrile). ..

Article Title: Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study
Article Snippet: .. Cyclotides in the eluted fractions were further purified using semi-preparative HPLC (Agilent 1220 LC, Agilent Technologies, Santa Clara, CA, USA). .. Separation was achieved using a linear gradient from 10–70% B over 25 mins, where A was 0.1% TFA in water and B 80% acetonitrile containing 0.1% TFA, on a semi-preparative HPLC column (Phenomenex, UK; Jupiter C18 reverse phase, 300 A, 5 μm particle size, 9.4 × 250 mm) at a flow rate of 4 mL/min.

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties
Article Snippet: .. After thin-layer chromatography in ethyl acetate: formic acid :water (44:3:3) foxicin A (Rf 0.41) was further purified by semi-preparative HPLC (Agilent Technologies), equipped with a Zorbax B-C18 (9.4 × 150 mm) pre-column and Zorbax B-C18 (9.4 mm × 20 mm) main column with acetonitrile + 0.5% acetic acid as buffer A and water + 0.5% acetic acid as buffer B and a flow rate of 2 mL/min. ..

Article Title: Bis-butanediol-mercapturic acid (bis-BDMA) as a urinary biomarker of metabolic activation of butadiene to its ultimate carcinogenic species
Article Snippet: .. Bis -BDMA was isolated by semi-preparative HPLC using an Agilent 1100 HPLC system interfaced to a DAD UV detector (Agilent Technologies, Santa Clara, CA). .. HPLC separation was achieved with a Synergi Hydro RP column (250 × 10.00mm; 4 μ) (Phenomenex, Torrance, CA) using isocratic elution with 6% acetonitrile in 0.1% trifluoroacetic acid/water.

Article Title: Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination
Article Snippet: .. The reaction mixture was stirred at 50 °C for 1 h. The product was purified by semi-preparative HPLC (conditions: Agilent Eclipse plus C18 column (9.4 × 250 mm, 5 μm), mobile phase: 5 - 50% acetonitrile in water (0.1% TFA), with a flow rate of 4.0 mL/min.) ..

Article Title: Multi-arm Nanoconjugates for Cancer Cell-Targeted Delivery of Photosensitizers
Article Snippet: .. The modified RGD-mal was purified with semi-preparative HPLC using an Agilent ZORBAX C18 column (9.4×250 mm, 5 μm, Agilent Technologies, Santa Clara, CA, USA). .. The nanoconjugates were synthesized according to the scheme in .

Article Title: Crown Procyanidin Tetramer: A Procyanidin with an Unusual Cyclic Skeleton with a Potent Protective Effect against Amyloid-β-Induced Toxicity
Article Snippet: .. The semi-preparative HPLC was performed on an Agilent 1260 Infinity system (Agilent, Les Ulis, France) containing a quaternary pump (1260 Infinity), a compartment thermostat column (1290 Infinity), a sample injector (1260 Standard Autosampler), and a diode array detector (1260 DAD VL+). ..

Article Title: Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies
Article Snippet: .. Each fraction was analyzed by HPLC and dissolved in MeOH and finally purified by semi-preparative HPLC to give the resulting CRM A, as identified by 1 H, 13C NMR and HR-ESI-MS. Analytical HPLC of CRM A was performed on the Agilent 1260 Infinity System with an Agilent Zorbax SB-C18 column (150 × 4.6 mm, 5 μm) eluted with a linear gradient of 0% to 80% solvent B over 20 min, followed by 80% to 100% solvent B in 30 s, and then eluted with 100% solvent B in 4.5 min, at a flow rate of 1.0 mL/min using UV detection at both 210 nm and 254 nm. ..

Flow Cytometry:

Article Title: Mining of the Pyrrolamide Antibiotics Analogs in Streptomyces netropsis Reveals the Amidohydrolase-Dependent "Iterative Strategy" Underlying the Pyrrole Polymerization
Article Snippet: .. Purification of pyrrolamides was performed by semi-preparative HPLC of the crude extract on a Agilent ZORBAX SB-C18 column (5 µm, 9.4×250 mm) with a flow rate of 3 mL/min over a 35 min gradient (T = 0 min, 10% B; T = 20 min, 40% B; T = 25 min, 100% B; T = 35 min, 100%; solvent A, water; solvent B, acetonitrile). ..

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties
Article Snippet: .. After thin-layer chromatography in ethyl acetate: formic acid :water (44:3:3) foxicin A (Rf 0.41) was further purified by semi-preparative HPLC (Agilent Technologies), equipped with a Zorbax B-C18 (9.4 × 150 mm) pre-column and Zorbax B-C18 (9.4 mm × 20 mm) main column with acetonitrile + 0.5% acetic acid as buffer A and water + 0.5% acetic acid as buffer B and a flow rate of 2 mL/min. ..

Article Title: Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination
Article Snippet: .. The reaction mixture was stirred at 50 °C for 1 h. The product was purified by semi-preparative HPLC (conditions: Agilent Eclipse plus C18 column (9.4 × 250 mm, 5 μm), mobile phase: 5 - 50% acetonitrile in water (0.1% TFA), with a flow rate of 4.0 mL/min.) ..

Isolation:

Article Title: Bis-butanediol-mercapturic acid (bis-BDMA) as a urinary biomarker of metabolic activation of butadiene to its ultimate carcinogenic species
Article Snippet: .. Bis -BDMA was isolated by semi-preparative HPLC using an Agilent 1100 HPLC system interfaced to a DAD UV detector (Agilent Technologies, Santa Clara, CA). .. HPLC separation was achieved with a Synergi Hydro RP column (250 × 10.00mm; 4 μ) (Phenomenex, Torrance, CA) using isocratic elution with 6% acetonitrile in 0.1% trifluoroacetic acid/water.

Purification:

Article Title: Mining of the Pyrrolamide Antibiotics Analogs in Streptomyces netropsis Reveals the Amidohydrolase-Dependent "Iterative Strategy" Underlying the Pyrrole Polymerization
Article Snippet: .. Purification of pyrrolamides was performed by semi-preparative HPLC of the crude extract on a Agilent ZORBAX SB-C18 column (5 µm, 9.4×250 mm) with a flow rate of 3 mL/min over a 35 min gradient (T = 0 min, 10% B; T = 20 min, 40% B; T = 25 min, 100% B; T = 35 min, 100%; solvent A, water; solvent B, acetonitrile). ..

Article Title: Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study
Article Snippet: .. Cyclotides in the eluted fractions were further purified using semi-preparative HPLC (Agilent 1220 LC, Agilent Technologies, Santa Clara, CA, USA). .. Separation was achieved using a linear gradient from 10–70% B over 25 mins, where A was 0.1% TFA in water and B 80% acetonitrile containing 0.1% TFA, on a semi-preparative HPLC column (Phenomenex, UK; Jupiter C18 reverse phase, 300 A, 5 μm particle size, 9.4 × 250 mm) at a flow rate of 4 mL/min.

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties
Article Snippet: .. After thin-layer chromatography in ethyl acetate: formic acid :water (44:3:3) foxicin A (Rf 0.41) was further purified by semi-preparative HPLC (Agilent Technologies), equipped with a Zorbax B-C18 (9.4 × 150 mm) pre-column and Zorbax B-C18 (9.4 mm × 20 mm) main column with acetonitrile + 0.5% acetic acid as buffer A and water + 0.5% acetic acid as buffer B and a flow rate of 2 mL/min. ..

Article Title: Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination
Article Snippet: .. The reaction mixture was stirred at 50 °C for 1 h. The product was purified by semi-preparative HPLC (conditions: Agilent Eclipse plus C18 column (9.4 × 250 mm, 5 μm), mobile phase: 5 - 50% acetonitrile in water (0.1% TFA), with a flow rate of 4.0 mL/min.) ..

Article Title: Multi-arm Nanoconjugates for Cancer Cell-Targeted Delivery of Photosensitizers
Article Snippet: .. The modified RGD-mal was purified with semi-preparative HPLC using an Agilent ZORBAX C18 column (9.4×250 mm, 5 μm, Agilent Technologies, Santa Clara, CA, USA). .. The nanoconjugates were synthesized according to the scheme in .

Article Title: Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies
Article Snippet: .. Each fraction was analyzed by HPLC and dissolved in MeOH and finally purified by semi-preparative HPLC to give the resulting CRM A, as identified by 1 H, 13C NMR and HR-ESI-MS. Analytical HPLC of CRM A was performed on the Agilent 1260 Infinity System with an Agilent Zorbax SB-C18 column (150 × 4.6 mm, 5 μm) eluted with a linear gradient of 0% to 80% solvent B over 20 min, followed by 80% to 100% solvent B in 30 s, and then eluted with 100% solvent B in 4.5 min, at a flow rate of 1.0 mL/min using UV detection at both 210 nm and 254 nm. ..

Nuclear Magnetic Resonance:

Article Title: Activation and enhancement of caerulomycin A biosynthesis in marine-derived Actinoalloteichus sp. AHMU CJ021 by combinatorial genome mining strategies
Article Snippet: .. Each fraction was analyzed by HPLC and dissolved in MeOH and finally purified by semi-preparative HPLC to give the resulting CRM A, as identified by 1 H, 13C NMR and HR-ESI-MS. Analytical HPLC of CRM A was performed on the Agilent 1260 Infinity System with an Agilent Zorbax SB-C18 column (150 × 4.6 mm, 5 μm) eluted with a linear gradient of 0% to 80% solvent B over 20 min, followed by 80% to 100% solvent B in 30 s, and then eluted with 100% solvent B in 4.5 min, at a flow rate of 1.0 mL/min using UV detection at both 210 nm and 254 nm. ..

Thin Layer Chromatography:

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties
Article Snippet: .. After thin-layer chromatography in ethyl acetate: formic acid :water (44:3:3) foxicin A (Rf 0.41) was further purified by semi-preparative HPLC (Agilent Technologies), equipped with a Zorbax B-C18 (9.4 × 150 mm) pre-column and Zorbax B-C18 (9.4 mm × 20 mm) main column with acetonitrile + 0.5% acetic acid as buffer A and water + 0.5% acetic acid as buffer B and a flow rate of 2 mL/min. ..

Modification:

Article Title: Multi-arm Nanoconjugates for Cancer Cell-Targeted Delivery of Photosensitizers
Article Snippet: .. The modified RGD-mal was purified with semi-preparative HPLC using an Agilent ZORBAX C18 column (9.4×250 mm, 5 μm, Agilent Technologies, Santa Clara, CA, USA). .. The nanoconjugates were synthesized according to the scheme in .

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    Agilent technologies semi preparativerp hplc column
    Semi Preparativerp Hplc Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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