secondary antibody  (Bio-Rad)

 
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    Name:
    Secondary Antibody Goat Anti Rabbit Antibody Conjugated to Horseradish Peroxidase
    Description:
    Antibody goat anti rabbit antibody conjugated to horseradish peroxidase HRP lyophilized for shipping and long term storage education use only
    Catalog Number:
    1662408edu
    Price:
    None
    Category:
    ThINQ Investigation Kits for AP Biology
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    Structured Review

    Bio-Rad secondary antibody
    Secondary Antibody Goat Anti Rabbit Antibody Conjugated to Horseradish Peroxidase
    Antibody goat anti rabbit antibody conjugated to horseradish peroxidase HRP lyophilized for shipping and long term storage education use only
    https://www.bioz.com/result/secondary antibody/product/Bio-Rad
    Average 99 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    secondary antibody - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
    Article Snippet: .. For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA). .. Embryos were mounted between cover slips for viewing and analysed using Zeiss Axioplan 2 microscope (Thornwood, NY, USA).

    Incubation:

    Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
    Article Snippet: .. For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA). .. Embryos were mounted between cover slips for viewing and analysed using Zeiss Axioplan 2 microscope (Thornwood, NY, USA).

    Mouse Assay:

    Article Title: Cardiac-Oxidized Antigens Are Targets of Immune Recognition by Antibodies and Potential Molecular Determinants in Chagas Disease Pathogenesis
    Article Snippet: .. Membranes were probed with sera from normal or chagasic rats, mice or human patients (1∶100 dilution) followed by HRP-conjugated secondary antibody (1∶5000, BioRad), and signal was detected by an ECL plus chemiluminiscence detection system (GE-Healthcare). .. Image analysis 2D gels (n = 4/group) or Western blots (n = 4/group) were digitalized on a ProXPRESS Proteomic Imaging System (Perkin Elmer), and the images were analyzed on Progenesis SameSpotst™ software 2.0 (NonLinear Dynamics).

    Conjugation Assay:

    Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
    Article Snippet: .. Detection was performed by adding HRP-conjugated Type 3 detection antibodies (conjugated with LYNX rapid HRP antibody conjugation kit, Bio-Rad LNK002P) or by adding Type 3 antibodies followed by HRP-conjugated anti-tag secondary antibodies in HISPEC assay diluent (Bio-Rad BUF49A) for 1 h at RT. .. Plates were washed ten times with PBST, followed by the addition of QuantaBlu fluorogenic peroxidase substrate (Thermo Fisher Scientific #15169).

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  • 93
    Bio-Rad secondary antibodies against bovine igg1
    Titrated IgM, <t>IgG1,</t> and IgG2 antibody response in animals vaccinated with salivary gland extract (SGE), midgut extract (ME) or a combination of both SGE and ME (SGE+ME). Plates were coated with 0.5 μg/mL SGE (A,B) or ME (C,D) , respectively.
    Secondary Antibodies Against Bovine Igg1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary antibodies against bovine igg1/product/Bio-Rad
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    secondary antibodies against bovine igg1 - by Bioz Stars, 2020-09
    93/100 stars
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    91
    Bio-Rad texas red conjugated secondary antibodies
    Confocal and electron microscopy of rat chondrocytes immuno-cytochemically stained with COX-2 and caveolin <t>antibodies.</t> Cells were double stained with fluorescein isothiocyanate- and <t>Texas</t> <t>Red-conjugated</t> <t>secondary</t> antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under a confocal microscope at their respective wavelengths. For each experiment, a least 50 cells were examined and the presented images represent typical staining pattern for the majority of examined cells. a, Cav-3; b, COX-2; c, merged image of a and b; d and e; stained only with secondary antibodies without the prior treatment of either Cav-3 or COX-2 antibodies ( A ). Cells were double stained with different size of gold particle-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under an electron microscope. Arrow showed structure of caveolae. # and * represented COX-2 (15 nm particles) and Cav-3 (10 nm particles), respectively.
    Texas Red Conjugated Secondary Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/texas red conjugated secondary antibodies/product/Bio-Rad
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    texas red conjugated secondary antibodies - by Bioz Stars, 2020-09
    91/100 stars
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    85
    Bio-Rad goat anti mouse fluorescein labeled secondary mab
    Fluorescent microscopy images of A2780/AD cells incubated with UIC2 antibodies followed by <t>fluorescein</t> <t>labeled</t> <t>anti-mouse</t> IgG for 16 h at 4 (A) or 37°C (B), with conjugate 11 [P-(AP-FITC)-mAb] for 16h at 4 (C) or 37°C (D), and with conjugate 11 [P-(AP-FITC)-mAb] for 48h at 37°C (E) and conjugate 10 [P-(AP-FITC)] for 16 h at 37°C (F).
    Goat Anti Mouse Fluorescein Labeled Secondary Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse fluorescein labeled secondary mab/product/Bio-Rad
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse fluorescein labeled secondary mab - by Bioz Stars, 2020-09
    85/100 stars
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    89
    Bio-Rad secondary antibodies conjugated to alkaline phosphatase
    The effect of maternal diet on expression levels of Rrn3 transcription factor in the offspring. (A) A cartoon illustrating the role of Rrn3 in mediating extracellular signals <t>to</t> rDNA transcription by Pol I. (B) RT PCR analysis of Rrn3 transcript levels in 17 dpc and adult kidneys. RNA purified from kidneys was treated with DNase, reverse transcribed with random hexamer primers, and analyzed by qPCR with two different pairs of primers to the last exon of Rrn3 gene. Transcript levels were normalized to two control transcripts, Cypa and Lamc1 , data were combined, adjusted to NPD levels, and presented as mean ± SD. (C) Western blot analysis of Rrn3 protein levels in adult kidneys. Extracts from kidneys (male), 50 μg total protein per lane, were electrophoresed in SDS gels, transferred to PVDF membrane and probed consecutively with <t>antibodies</t> to Rrn3 and β-actin proteins. After incubation with <t>secondary</t> <t>antibody</t> <t>conjugated</t> with <t>alkaline</t> <t>phosphatase,</t> membranes were developed with NBT/BCIP substrate. Representative membrane is shown. (D) Densitometry of Rrn3 band intensities. Western blot membranes were scanned and Rrn3 and β-actin band densities were measured using ImageJ64 software. Graph shows Rrn3/β-actin ratios adjusted to NPD levels, mean ± SD, n = 6.
    Secondary Antibodies Conjugated To Alkaline Phosphatase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary antibodies conjugated to alkaline phosphatase/product/Bio-Rad
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    secondary antibodies conjugated to alkaline phosphatase - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results


    Titrated IgM, IgG1, and IgG2 antibody response in animals vaccinated with salivary gland extract (SGE), midgut extract (ME) or a combination of both SGE and ME (SGE+ME). Plates were coated with 0.5 μg/mL SGE (A,B) or ME (C,D) , respectively.

    Journal: Frontiers in Physiology

    Article Title: Preliminary Evaluation of Tick Protein Extracts and Recombinant Ferritin 2 as Anti-tick Vaccines Targeting Ixodes ricinus in Cattle

    doi: 10.3389/fphys.2018.01696

    Figure Lengend Snippet: Titrated IgM, IgG1, and IgG2 antibody response in animals vaccinated with salivary gland extract (SGE), midgut extract (ME) or a combination of both SGE and ME (SGE+ME). Plates were coated with 0.5 μg/mL SGE (A,B) or ME (C,D) , respectively.

    Article Snippet: The plates were then washed, blocked with 1% fetal bovine serum in PBS, incubated with the sera dilutions followed by specific secondary antibodies against bovine IgG1, IgG2, and IgM conjugated with horseradish peroxidase (Bio-Rad).

    Techniques:

    Confocal and electron microscopy of rat chondrocytes immuno-cytochemically stained with COX-2 and caveolin antibodies. Cells were double stained with fluorescein isothiocyanate- and Texas Red-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under a confocal microscope at their respective wavelengths. For each experiment, a least 50 cells were examined and the presented images represent typical staining pattern for the majority of examined cells. a, Cav-3; b, COX-2; c, merged image of a and b; d and e; stained only with secondary antibodies without the prior treatment of either Cav-3 or COX-2 antibodies ( A ). Cells were double stained with different size of gold particle-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under an electron microscope. Arrow showed structure of caveolae. # and * represented COX-2 (15 nm particles) and Cav-3 (10 nm particles), respectively.

    Journal: Journal of Korean Medical Science

    Article Title: Evidence for Cyclooxygenase-2 Association with Caveolin-3 in Primary Cultured Rat Chondrocytes

    doi: 10.3346/jkms.2006.21.1.100

    Figure Lengend Snippet: Confocal and electron microscopy of rat chondrocytes immuno-cytochemically stained with COX-2 and caveolin antibodies. Cells were double stained with fluorescein isothiocyanate- and Texas Red-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under a confocal microscope at their respective wavelengths. For each experiment, a least 50 cells were examined and the presented images represent typical staining pattern for the majority of examined cells. a, Cav-3; b, COX-2; c, merged image of a and b; d and e; stained only with secondary antibodies without the prior treatment of either Cav-3 or COX-2 antibodies ( A ). Cells were double stained with different size of gold particle-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under an electron microscope. Arrow showed structure of caveolae. # and * represented COX-2 (15 nm particles) and Cav-3 (10 nm particles), respectively.

    Article Snippet: After washing, cells were double stained with FITC- and Texas Red-conjugated secondary antibodies for 30 min. After washing, samples were examined at the appropriate wavelengths under a Bio-Rad MRC 1000 confocal microscope (Hercules, CA, U.S.A.).

    Techniques: Electron Microscopy, Staining, Microscopy

    Fluorescent microscopy images of A2780/AD cells incubated with UIC2 antibodies followed by fluorescein labeled anti-mouse IgG for 16 h at 4 (A) or 37°C (B), with conjugate 11 [P-(AP-FITC)-mAb] for 16h at 4 (C) or 37°C (D), and with conjugate 11 [P-(AP-FITC)-mAb] for 48h at 37°C (E) and conjugate 10 [P-(AP-FITC)] for 16 h at 37°C (F).

    Journal: Macromolecular bioscience

    Article Title: Targeting of Multidrug-Resistant Human Ovarian Carcinoma Cells With Anti-P-Glycoprotein Antibody Conjugates

    doi: 10.1002/mabi.201100350

    Figure Lengend Snippet: Fluorescent microscopy images of A2780/AD cells incubated with UIC2 antibodies followed by fluorescein labeled anti-mouse IgG for 16 h at 4 (A) or 37°C (B), with conjugate 11 [P-(AP-FITC)-mAb] for 16h at 4 (C) or 37°C (D), and with conjugate 11 [P-(AP-FITC)-mAb] for 48h at 37°C (E) and conjugate 10 [P-(AP-FITC)] for 16 h at 37°C (F).

    Article Snippet: The localization of conjugates 10 [P-(AP-FITC)] and 11 [P-(AP-FITC)-mAb], and UIC2 visualized with goat anti-mouse fluorescein labeled secondary mAb were determined on a Bio-Rad MRC 600 laser scanning confocal imaging system.

    Techniques: Microscopy, Incubation, Labeling

    Flow cytometry analysis of P-glycoprotein expression on the human ovarian cancer cell line A2780/AD: (—) A2780 control cells; (···) A2780 control cells exposed to fluorescein labeled anti-mouse IgG; (---) A2780/AD cells exposed to P-(AP-FITC)-mAb; and ( ) A2780/AD cells exposed to UIC2 mAb followed by fluorescein labeled anti-mouse IgG. FL1-Height is the relative intensity of fluorescein.

    Journal: Macromolecular bioscience

    Article Title: Targeting of Multidrug-Resistant Human Ovarian Carcinoma Cells With Anti-P-Glycoprotein Antibody Conjugates

    doi: 10.1002/mabi.201100350

    Figure Lengend Snippet: Flow cytometry analysis of P-glycoprotein expression on the human ovarian cancer cell line A2780/AD: (—) A2780 control cells; (···) A2780 control cells exposed to fluorescein labeled anti-mouse IgG; (---) A2780/AD cells exposed to P-(AP-FITC)-mAb; and ( ) A2780/AD cells exposed to UIC2 mAb followed by fluorescein labeled anti-mouse IgG. FL1-Height is the relative intensity of fluorescein.

    Article Snippet: The localization of conjugates 10 [P-(AP-FITC)] and 11 [P-(AP-FITC)-mAb], and UIC2 visualized with goat anti-mouse fluorescein labeled secondary mAb were determined on a Bio-Rad MRC 600 laser scanning confocal imaging system.

    Techniques: Flow Cytometry, Cytometry, Expressing, Labeling

    The effect of maternal diet on expression levels of Rrn3 transcription factor in the offspring. (A) A cartoon illustrating the role of Rrn3 in mediating extracellular signals to rDNA transcription by Pol I. (B) RT PCR analysis of Rrn3 transcript levels in 17 dpc and adult kidneys. RNA purified from kidneys was treated with DNase, reverse transcribed with random hexamer primers, and analyzed by qPCR with two different pairs of primers to the last exon of Rrn3 gene. Transcript levels were normalized to two control transcripts, Cypa and Lamc1 , data were combined, adjusted to NPD levels, and presented as mean ± SD. (C) Western blot analysis of Rrn3 protein levels in adult kidneys. Extracts from kidneys (male), 50 μg total protein per lane, were electrophoresed in SDS gels, transferred to PVDF membrane and probed consecutively with antibodies to Rrn3 and β-actin proteins. After incubation with secondary antibody conjugated with alkaline phosphatase, membranes were developed with NBT/BCIP substrate. Representative membrane is shown. (D) Densitometry of Rrn3 band intensities. Western blot membranes were scanned and Rrn3 and β-actin band densities were measured using ImageJ64 software. Graph shows Rrn3/β-actin ratios adjusted to NPD levels, mean ± SD, n = 6.

    Journal: Biochimica et Biophysica Acta

    Article Title: Regulation of ribosomal RNA expression across the lifespan is fine-tuned by maternal diet before implantation

    doi: 10.1016/j.bbagrm.2016.04.001

    Figure Lengend Snippet: The effect of maternal diet on expression levels of Rrn3 transcription factor in the offspring. (A) A cartoon illustrating the role of Rrn3 in mediating extracellular signals to rDNA transcription by Pol I. (B) RT PCR analysis of Rrn3 transcript levels in 17 dpc and adult kidneys. RNA purified from kidneys was treated with DNase, reverse transcribed with random hexamer primers, and analyzed by qPCR with two different pairs of primers to the last exon of Rrn3 gene. Transcript levels were normalized to two control transcripts, Cypa and Lamc1 , data were combined, adjusted to NPD levels, and presented as mean ± SD. (C) Western blot analysis of Rrn3 protein levels in adult kidneys. Extracts from kidneys (male), 50 μg total protein per lane, were electrophoresed in SDS gels, transferred to PVDF membrane and probed consecutively with antibodies to Rrn3 and β-actin proteins. After incubation with secondary antibody conjugated with alkaline phosphatase, membranes were developed with NBT/BCIP substrate. Representative membrane is shown. (D) Densitometry of Rrn3 band intensities. Western blot membranes were scanned and Rrn3 and β-actin band densities were measured using ImageJ64 software. Graph shows Rrn3/β-actin ratios adjusted to NPD levels, mean ± SD, n = 6.

    Article Snippet: After washes, secondary antibodies conjugated to alkaline phosphatase (Bio Rad, Hercules, CA, USA) were added for 1 h at room temperature.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Purification, Random Hexamer Labeling, Real-time Polymerase Chain Reaction, Western Blot, Incubation, Software