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Bio-Rad secondary antibody
Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibody/product/Bio-Rad
Average 90 stars, based on 57 article reviews
Price from $9.99 to $1999.99
secondary antibody - by Bioz Stars, 2020-02
90/100 stars

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Related Articles

Blocking Assay:

Article Title: Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats
Article Snippet: After transferring and blocking to a polyvinylidene difluoride (PVDF) membrane, the membrane was incubated for 2 h with anti-histone H3 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or H4 antibody (Santa Cruz) at 1:500. .. After washing and incubation for 2 h with peroxidase-labeled secondary antibody diluted 1:1000 in T-BST, The signal was obtained using a Bio-Rad Molecular Imager ChemiDocMP and quantified by Image Lab version 5.0 software (Bio-Rad, Richmond, CA, USA).

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: After washing and blocking, the ErbB2/trastuzumab complex was formed by adding trastuzumab at concentrations from 32 µg/mL to 0.06 ng/mL in PBST spiked with 10% NHS. .. The Type 3 antibody AbD25279 (Bio-Rad HCA263) was added at 2 µg/mL in HISPEC assay diluent, either directly HRP-conjugated or followed by a commercially available HRP-conjugated secondary antibody using the dilutions recommended by the suppliers: anti-His:HRP (1:2,000, Bio-Rad MCA5995P), anti-BAP:HRP (1:5,000, Bio-Rad HCA275P) and anti-FLAG:HRP (1:20,000, Sigma A8592).

Article Title: Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs
Article Snippet: Following a wash (PBS with 0.05% Tween 20), wells were blocked for 1 h at 37°C with blocking buffer (PBS with 1% BSA and 0.05% Tween 20). .. This was followed by detection using 2.5 μg/ml biotinylated mouse anti-bovine TNFα or IFNγ secondary antibody (AbD Serotec), 1:200 streptavidin-HRP conjugate (Invitrogen), and 1-Step turbo TMB-ELISA substrate solution (Thermo Scientific, Rockford, IL).

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: After blocking with 5% (w/v) BSA in PBST for 1 hour (h) at room temperature (RT), the drug target/drug complex was formed by adding the drug to the wells coated with the corresponding drug target and incubated for 1 h at RT. .. Detection was performed by adding HRP-conjugated Type 3 detection antibodies (conjugated with LYNX rapid HRP antibody conjugation kit, Bio-Rad LNK002P) or by adding Type 3 antibodies followed by HRP-conjugated anti-tag secondary antibodies in HISPEC assay diluent (Bio-Rad BUF49A) for 1 h at RT.

Enzyme-linked Immunosorbent Assay:

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: Paragraph title: General ELISA information ... Detection was performed by adding HRP-conjugated Type 3 detection antibodies (conjugated with LYNX rapid HRP antibody conjugation kit, Bio-Rad LNK002P) or by adding Type 3 antibodies followed by HRP-conjugated anti-tag secondary antibodies in HISPEC assay diluent (Bio-Rad BUF49A) for 1 h at RT.

Article Title: Opposing Roles of Integrin ?6A?1 and Dystroglycan in Laminin-mediated Extracellular Signal-regulated Kinase Activation
Article Snippet: Serial dilutions of laminin-1, -5, and -10/11 were added and incubated for 2 h. After washing, bound ligand was detected with specific antisera against laminin α1 chain , laminin α3 chain (2B10), or 4C7 mAb against laminin α5 chain ( ) diluted to give a maximal absorbance of 1.2–1.6 in enzyme-linked immunosorbent assay. .. After a further wash, bound antibodies were detected by horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA) followed by addition of 1 mg/ml 5-amino-2-hydroxybenzoic acid (Sigma-Aldrich), 0.001% H2 O2 .

Sandwich ELISA:

Article Title: Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs
Article Snippet: Paragraph title: Sandwich ELISA ... This was followed by detection using 2.5 μg/ml biotinylated mouse anti-bovine TNFα or IFNγ secondary antibody (AbD Serotec), 1:200 streptavidin-HRP conjugate (Invitrogen), and 1-Step turbo TMB-ELISA substrate solution (Thermo Scientific, Rockford, IL).

Incubation:

Article Title: Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats
Article Snippet: .. After washing and incubation for 2 h with peroxidase-labeled secondary antibody diluted 1:1000 in T-BST, The signal was obtained using a Bio-Rad Molecular Imager ChemiDocMP and quantified by Image Lab version 5.0 software (Bio-Rad, Richmond, CA, USA). .. Statistics For most parameters, statistical analysis was performed using a Mann–Whitney U test; otherwise, ANOVA with the Bonferroni post hoc test for multiple comparisons of two or four groups was used as indicated.

Article Title: Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs
Article Snippet: Standards and samples diluted in wash buffer were added to the plate and incubated for 1 h at 37°C. .. This was followed by detection using 2.5 μg/ml biotinylated mouse anti-bovine TNFα or IFNγ secondary antibody (AbD Serotec), 1:200 streptavidin-HRP conjugate (Invitrogen), and 1-Step turbo TMB-ELISA substrate solution (Thermo Scientific, Rockford, IL).

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: After blocking with 5% (w/v) BSA in PBST for 1 hour (h) at room temperature (RT), the drug target/drug complex was formed by adding the drug to the wells coated with the corresponding drug target and incubated for 1 h at RT. .. Detection was performed by adding HRP-conjugated Type 3 detection antibodies (conjugated with LYNX rapid HRP antibody conjugation kit, Bio-Rad LNK002P) or by adding Type 3 antibodies followed by HRP-conjugated anti-tag secondary antibodies in HISPEC assay diluent (Bio-Rad BUF49A) for 1 h at RT.

Article Title: Decreased levels of baseline and drug-induced tubulin polymerisation are hallmarks of resistance to taxanes in ovarian cancer cells and are associated with epithelial-to-mesenchymal transition
Article Snippet: .. Membranes were then incubated with species-appropriate horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature prior to detection using the Clarity Western ECL substrate (Bio-Rad Laboratories, Hercules, CA, USA). .. Migration assay Wound-healing assays were performed in triplicate measurements using the CytoSelect 24-well Wound-Healing Assay (Cell Biolabs, Inc. San Diego, CA, USA) according to the manufacturer’s instructions.

Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
Article Snippet: .. For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA). .. Embryos were mounted between cover slips for viewing and analysed using Zeiss Axioplan 2 microscope (Thornwood, NY, USA).

Article Title: Opposing Roles of Integrin ?6A?1 and Dystroglycan in Laminin-mediated Extracellular Signal-regulated Kinase Activation
Article Snippet: Serial dilutions of laminin-1, -5, and -10/11 were added and incubated for 2 h. After washing, bound ligand was detected with specific antisera against laminin α1 chain , laminin α3 chain (2B10), or 4C7 mAb against laminin α5 chain ( ) diluted to give a maximal absorbance of 1.2–1.6 in enzyme-linked immunosorbent assay. .. After a further wash, bound antibodies were detected by horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA) followed by addition of 1 mg/ml 5-amino-2-hydroxybenzoic acid (Sigma-Aldrich), 0.001% H2 O2 .

Expressing:

Article Title: Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration
Article Snippet: A secondary antibody-peroxidase conjugate was used for visualisation (Bio-Rad). .. The expression of the protein of interest was normalised to GAPDH.

Western Blot:

Article Title: Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats
Article Snippet: Paragraph title: 4.9. Western Blotting ... After washing and incubation for 2 h with peroxidase-labeled secondary antibody diluted 1:1000 in T-BST, The signal was obtained using a Bio-Rad Molecular Imager ChemiDocMP and quantified by Image Lab version 5.0 software (Bio-Rad, Richmond, CA, USA).

Article Title: The Bile Acid Sensor FXR Is Required for Immune-Regulatory Activities of TLR-9 in Intestinal Inflammation
Article Snippet: Paragraph title: Western Blotting ... The anti-immunoglobulin G horseradish peroxidase conjugate (Bio-Rad) was used as the secondary antibody and specific protein bands were visualized using the Super Signal West Dura reagent (Pierce), following the manufacturer's suggested protocol.

Article Title: Decreased levels of baseline and drug-induced tubulin polymerisation are hallmarks of resistance to taxanes in ovarian cancer cells and are associated with epithelial-to-mesenchymal transition
Article Snippet: .. Membranes were then incubated with species-appropriate horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature prior to detection using the Clarity Western ECL substrate (Bio-Rad Laboratories, Hercules, CA, USA). .. Migration assay Wound-healing assays were performed in triplicate measurements using the CytoSelect 24-well Wound-Healing Assay (Cell Biolabs, Inc. San Diego, CA, USA) according to the manufacturer’s instructions.

Article Title: Therapeutic potential of ERK5 targeting in triple negative breast cancer
Article Snippet: The antibodies utilized in the different Western blot analyses were: pCHK1Ser296 , pCHK2Tyr68 , pH2AXSer139 , caspase-7, caspase-9, COX IV and CDK9 (Cell Signalling Technology, Danvers, MA), GAPDH, PARP, pCDK1Tyr15 , AIF, BCL2, MCL-1, Wee1 and ERK1/2 (Santa Cruz Biotechnology, Santa Cruz, CA); caspase-3, caspase-8, BCLXL , XIAP, cIAP1, cIAP2, cytochrome C, SMAC/DIABLO, cyclin A, pRbSer807/811 , pRbSer780 and Bid (BD Biosciences, San Jose, CA). .. Horseradish peroxidase–conjugated secondary antibodies were from Bio-Rad (Hercules, CA, USA).

Article Title: Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration
Article Snippet: Paragraph title: Western blot ... A secondary antibody-peroxidase conjugate was used for visualisation (Bio-Rad).

Conjugation Assay:

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: .. Detection was performed by adding HRP-conjugated Type 3 detection antibodies (conjugated with LYNX rapid HRP antibody conjugation kit, Bio-Rad LNK002P) or by adding Type 3 antibodies followed by HRP-conjugated anti-tag secondary antibodies in HISPEC assay diluent (Bio-Rad BUF49A) for 1 h at RT. .. Plates were washed ten times with PBST, followed by the addition of QuantaBlu fluorogenic peroxidase substrate (Thermo Fisher Scientific #15169).

Immunohistochemistry:

Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
Article Snippet: Paragraph title: Cryosectioning and immunohistochemistry ... For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA).

Protease Inhibitor:

Article Title: Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration
Article Snippet: Retinas were collected into Cellytic M buffer (Sigma-Aldrich) containing a Protease Inhibitor Cocktail (Sigma-Aldrich). .. A secondary antibody-peroxidase conjugate was used for visualisation (Bio-Rad).

Article Title: Involvement of urokinase receptor in the cross-talk between human hematopoietic stem cells and bone marrow microenvironment
Article Snippet: Rabbit anti-actin, mouse anti-tubulin antibodies, hydrocortisone sodium hemisuccinate, the protease inhibitor cocktail were from Sigma-Aldrich (St. Louis, MO). .. Polyclonal anti-caspase3 and anti-VN antibodies, the monoclonal anti-PARP1 and anti-β1 integrin antibodies were purchased from Santa Cruz (Santa Cruz, CA); horseradish peroxidase-conjugated secondary antibodies from Bio-Rad (Hercules, CA) and FITC anti-mouse IgG from Jackson Lab. (West Grove, PA).

Transferring:

Article Title: Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats
Article Snippet: After transferring and blocking to a polyvinylidene difluoride (PVDF) membrane, the membrane was incubated for 2 h with anti-histone H3 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or H4 antibody (Santa Cruz) at 1:500. .. After washing and incubation for 2 h with peroxidase-labeled secondary antibody diluted 1:1000 in T-BST, The signal was obtained using a Bio-Rad Molecular Imager ChemiDocMP and quantified by Image Lab version 5.0 software (Bio-Rad, Richmond, CA, USA).

Sequencing:

Article Title: Involvement of urokinase receptor in the cross-talk between human hematopoietic stem cells and bone marrow microenvironment
Article Snippet: The rabbit antibody, recognizing the SRSRY sequence of uPAR [ ], and uPAR84-95 (AVTYSRSRYLEC) and scrambled (TLVEYYSRASCR) peptides were purchased from PRIMM (Milan, Italy). .. Polyclonal anti-caspase3 and anti-VN antibodies, the monoclonal anti-PARP1 and anti-β1 integrin antibodies were purchased from Santa Cruz (Santa Cruz, CA); horseradish peroxidase-conjugated secondary antibodies from Bio-Rad (Hercules, CA) and FITC anti-mouse IgG from Jackson Lab. (West Grove, PA).

Affinity Purification:

Article Title: Homologs of PROTEIN TARGETING TO STARCH Control Starch Granule Initiation in Arabidopsis Leaves [OPEN]
Article Snippet: Antibodies specific for PTST2 were affinity purified from the antisera using NHS-activated Sepharose beads conjugated to the recombinant PTST2 protein. .. Proteins were detected with either chemiluminescence, using horseradish peroxidase-coupled secondary antibodies (Bio-Rad), or with IR fluorescence.

Recombinant:

Article Title: Homologs of PROTEIN TARGETING TO STARCH Control Starch Granule Initiation in Arabidopsis Leaves [OPEN]
Article Snippet: Antibodies specific for PTST2 were affinity purified from the antisera using NHS-activated Sepharose beads conjugated to the recombinant PTST2 protein. .. Proteins were detected with either chemiluminescence, using horseradish peroxidase-coupled secondary antibodies (Bio-Rad), or with IR fluorescence.

Fluorescence:

Article Title: Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics
Article Snippet: Detection was performed by adding HRP-conjugated Type 3 detection antibodies (conjugated with LYNX rapid HRP antibody conjugation kit, Bio-Rad LNK002P) or by adding Type 3 antibodies followed by HRP-conjugated anti-tag secondary antibodies in HISPEC assay diluent (Bio-Rad BUF49A) for 1 h at RT. .. Fluorescence signal was recorded after 30 min on a photometer (Tecan Infinite; excitation at 320 ± 25 nm, emission at 430 ± 35 nm).

Article Title: Homologs of PROTEIN TARGETING TO STARCH Control Starch Granule Initiation in Arabidopsis Leaves [OPEN]
Article Snippet: .. Proteins were detected with either chemiluminescence, using horseradish peroxidase-coupled secondary antibodies (Bio-Rad), or with IR fluorescence. .. For IR fluorescence, IR800-conjugated secondary antibodies and an Odyssey CLx detection system (Li-Cor) were used.

Magnetic Beads:

Article Title: Involvement of urokinase receptor in the cross-talk between human hematopoietic stem cells and bone marrow microenvironment
Article Snippet: Polyclonal anti-caspase3 and anti-VN antibodies, the monoclonal anti-PARP1 and anti-β1 integrin antibodies were purchased from Santa Cruz (Santa Cruz, CA); horseradish peroxidase-conjugated secondary antibodies from Bio-Rad (Hercules, CA) and FITC anti-mouse IgG from Jackson Lab. (West Grove, PA). .. Lymphocyte separation medium was from Life Technologies (Gaithersburg, MD); anti-CD3 Abs and IgG-conjugated magnetic beads for immunedepletion were from Life Technologies (Carlsbad, CA).

Microscopy:

Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
Article Snippet: For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA). .. Embryos were mounted between cover slips for viewing and analysed using Zeiss Axioplan 2 microscope (Thornwood, NY, USA).

Purification:

Article Title: Opposing Roles of Integrin ?6A?1 and Dystroglycan in Laminin-mediated Extracellular Signal-regulated Kinase Activation
Article Snippet: Culture supernatant was passed over a wheat germ agglutinin-Sepharose column (Amersham Biosciences) followed by purification on a laminin-1 affinity column as described previously ( ). .. After a further wash, bound antibodies were detected by horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA) followed by addition of 1 mg/ml 5-amino-2-hydroxybenzoic acid (Sigma-Aldrich), 0.001% H2 O2 .

Protein Extraction:

Article Title: Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats
Article Snippet: Each 50 mg of spleen tissue was homogenized with protein extraction solution (iNtRon biotechnology, Sungnam, Korea) according to the manufacturer’s instructions. .. After washing and incubation for 2 h with peroxidase-labeled secondary antibody diluted 1:1000 in T-BST, The signal was obtained using a Bio-Rad Molecular Imager ChemiDocMP and quantified by Image Lab version 5.0 software (Bio-Rad, Richmond, CA, USA).

Article Title: Homologs of PROTEIN TARGETING TO STARCH Control Starch Granule Initiation in Arabidopsis Leaves [OPEN]
Article Snippet: Paragraph title: Protein Extraction and Immunoblotting ... Proteins were detected with either chemiluminescence, using horseradish peroxidase-coupled secondary antibodies (Bio-Rad), or with IR fluorescence.

Affinity Column:

Article Title: Opposing Roles of Integrin ?6A?1 and Dystroglycan in Laminin-mediated Extracellular Signal-regulated Kinase Activation
Article Snippet: Culture supernatant was passed over a wheat germ agglutinin-Sepharose column (Amersham Biosciences) followed by purification on a laminin-1 affinity column as described previously ( ). .. After a further wash, bound antibodies were detected by horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA) followed by addition of 1 mg/ml 5-amino-2-hydroxybenzoic acid (Sigma-Aldrich), 0.001% H2 O2 .

IA:

Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
Article Snippet: Monoclonal antibodies F59 (antislow MyHC; 1 : 10) and EB165 (antifast MyLC; 1 : 200) were obtained from Developmental Studies Hybridoma Bank (University of Iowa, IA, USA). .. For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA).

Mouse Assay:

Article Title: Cardiac-Oxidized Antigens Are Targets of Immune Recognition by Antibodies and Potential Molecular Determinants in Chagas Disease Pathogenesis
Article Snippet: .. Membranes were probed with sera from normal or chagasic rats, mice or human patients (1∶100 dilution) followed by HRP-conjugated secondary antibody (1∶5000, BioRad), and signal was detected by an ECL plus chemiluminiscence detection system (GE-Healthcare). .. Image analysis 2D gels (n = 4/group) or Western blots (n = 4/group) were digitalized on a ProXPRESS Proteomic Imaging System (Perkin Elmer), and the images were analyzed on Progenesis SameSpotst™ software 2.0 (NonLinear Dynamics).

SDS Page:

Article Title: The Bile Acid Sensor FXR Is Required for Immune-Regulatory Activities of TLR-9 in Intestinal Inflammation
Article Snippet: Western Blotting Total proteins from Raw264.7 cells were separated by SDS PAGE, transferred to nitrocellulose membranes (Bio-Rad) and probed with primary antibodies FXR (Abcam), IRF-7 (SantaCruz) and α-tubulin (Sigma). .. The anti-immunoglobulin G horseradish peroxidase conjugate (Bio-Rad) was used as the secondary antibody and specific protein bands were visualized using the Super Signal West Dura reagent (Pierce), following the manufacturer's suggested protocol.

Software:

Article Title: Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats
Article Snippet: .. After washing and incubation for 2 h with peroxidase-labeled secondary antibody diluted 1:1000 in T-BST, The signal was obtained using a Bio-Rad Molecular Imager ChemiDocMP and quantified by Image Lab version 5.0 software (Bio-Rad, Richmond, CA, USA). .. Statistics For most parameters, statistical analysis was performed using a Mann–Whitney U test; otherwise, ANOVA with the Bonferroni post hoc test for multiple comparisons of two or four groups was used as indicated.

Article Title: The Bile Acid Sensor FXR Is Required for Immune-Regulatory Activities of TLR-9 in Intestinal Inflammation
Article Snippet: The anti-immunoglobulin G horseradish peroxidase conjugate (Bio-Rad) was used as the secondary antibody and specific protein bands were visualized using the Super Signal West Dura reagent (Pierce), following the manufacturer's suggested protocol. .. Films were scanned and the densitometry analysis performed using the Image J software (NIH, Bethesda, MD, USA).

Article Title: Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration
Article Snippet: A secondary antibody-peroxidase conjugate was used for visualisation (Bio-Rad). .. The protein was visualised with chemiluminescence using a Clarity Western ECL kit (Bio-Rad) and images were captured and analysed using a Chemidoc MP with Image Lab software (Bio-Rad).

In Vitro:

Article Title: Cardiac-Oxidized Antigens Are Targets of Immune Recognition by Antibodies and Potential Molecular Determinants in Chagas Disease Pathogenesis
Article Snippet: Immune recognition of cardiac proteins by chagasic sera Protein homogenates of cardiomyocytes or heart tissue (normal and in vitro oxidized) were resolved by 1D- or 2D-GE, and transferred to PVDF membranes, as above. .. Membranes were probed with sera from normal or chagasic rats, mice or human patients (1∶100 dilution) followed by HRP-conjugated secondary antibody (1∶5000, BioRad), and signal was detected by an ECL plus chemiluminiscence detection system (GE-Healthcare).

Staining:

Article Title: Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos
Article Snippet: .. For brown staining, embryos were incubated with peroxidase-tagged secondary antirabbit antibodies (1 : 200) (BioRad, Hercules, CA, USA) and stained with Fast DAB (Sigma, CA, USA). .. Embryos were mounted between cover slips for viewing and analysed using Zeiss Axioplan 2 microscope (Thornwood, NY, USA).

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  • 87
    Bio-Rad secondary antibodies against bovine igg1
    Titrated IgM, <t>IgG1,</t> and IgG2 antibody response in animals vaccinated with salivary gland extract (SGE), midgut extract (ME) or a combination of both SGE and ME (SGE+ME). Plates were coated with 0.5 μg/mL SGE (A,B) or ME (C,D) , respectively.
    Secondary Antibodies Against Bovine Igg1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary antibodies against bovine igg1/product/Bio-Rad
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    secondary antibodies against bovine igg1 - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    90
    Bio-Rad goat anti mouse igg h l hrp conjugate affinity purified secondary antibody
    Adenoviruses expressing capsid-incorporated SP70 epitope elicited SP70-specific humoral immune responses. (A) Immunization regimen showing when immunizations were performed and when sera were collected for antibodies assay and passive protection experiment. (B) Western blot of purified SP70-GST protein (lane 1, 2, 3) and cell extracts from EV71-infected Vero cells (lane 4, 5, 6, 7). The proteins were transferred onto PVDF and reacted with mice antisera raised against R1SP70A3 (lane 1, 4), R2SP70A3 (lane 2, 5), R7SP70A3 (lane 3, 6), or Ad3EGFP (lane 7) respectively. Mice antibodies were detected with an <t>HRP-linked</t> secondary antibody and the signals were developed by ECL. The position of the EV71 VP1 capsid protein is indicated. (C) Total anti-SP70 <t>IgG</t> levels were quantified by indirect ELISA analysis with 1 µg/ml of synthetic SP70 peptide as antigen. Post-prime and post-boost sera were collected at 2, 4, 6, and 8 weeks (wk) for ELISA binding assays. Error bars represent the standard errors of the means per group (n = 5). (D) The distribution of SP70-specific IgG subtypes in the sera. Sera from mice boosted with the antigens were collected at 8-week for ELISA isotype binding assays. ELISA plates were coated with synthetic SP70 peptide. The plates were then incubated with immunized mice sera (anti-EV71-08-02 sera diluted 1∶1000 and other groups antisera diluted 1∶10000). The binding was detected with HRP-conjugated secondary isotype specific antibodies. OD at 450 nm represents isotype-specific SP70 antibody levels in sera. Data are shown as mean ± SD for each group mice (n = 4) for one of two independent experiments. (E) Early humoral responses to SP70 were determined by ELISA with synthesized SP70 peptide at day 7 following the first immunization with hexon-modified Ad vectors. Data are shown as mean ± SD for each group mice (n = 4). (F) Total anti-EGFP IgG levels were quantified by indirect ELISA analysis with 1 µg/ml of recombinant EGFP as antigen. Post-prime and post-boost sera were collected at 2, 4, 6, and 8 weeks (wk) for ELISA binding assays. Data are shown as means ± SD per group (n = 5).
    Goat Anti Mouse Igg H L Hrp Conjugate Affinity Purified Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg h l hrp conjugate affinity purified secondary antibody/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l hrp conjugate affinity purified secondary antibody - by Bioz Stars, 2020-02
    90/100 stars
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    87
    Bio-Rad texas red conjugated secondary antibodies
    Confocal and electron microscopy of rat chondrocytes immuno-cytochemically stained with COX-2 and caveolin <t>antibodies.</t> Cells were double stained with fluorescein isothiocyanate- and <t>Texas</t> <t>Red-conjugated</t> <t>secondary</t> antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under a confocal microscope at their respective wavelengths. For each experiment, a least 50 cells were examined and the presented images represent typical staining pattern for the majority of examined cells. a, Cav-3; b, COX-2; c, merged image of a and b; d and e; stained only with secondary antibodies without the prior treatment of either Cav-3 or COX-2 antibodies ( A ). Cells were double stained with different size of gold particle-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under an electron microscope. Arrow showed structure of caveolae. # and * represented COX-2 (15 nm particles) and Cav-3 (10 nm particles), respectively.
    Texas Red Conjugated Secondary Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/texas red conjugated secondary antibodies/product/Bio-Rad
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    texas red conjugated secondary antibodies - by Bioz Stars, 2020-02
    87/100 stars
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    90
    Bio-Rad secondary antibodies conjugated to alkaline phosphatase
    The effect of maternal diet on expression levels of Rrn3 transcription factor in the offspring. (A) A cartoon illustrating the role of Rrn3 in mediating extracellular signals <t>to</t> rDNA transcription by Pol I. (B) RT PCR analysis of Rrn3 transcript levels in 17 dpc and adult kidneys. RNA purified from kidneys was treated with DNase, reverse transcribed with random hexamer primers, and analyzed by qPCR with two different pairs of primers to the last exon of Rrn3 gene. Transcript levels were normalized to two control transcripts, Cypa and Lamc1 , data were combined, adjusted to NPD levels, and presented as mean ± SD. (C) Western blot analysis of Rrn3 protein levels in adult kidneys. Extracts from kidneys (male), 50 μg total protein per lane, were electrophoresed in SDS gels, transferred to PVDF membrane and probed consecutively with <t>antibodies</t> to Rrn3 and β-actin proteins. After incubation with <t>secondary</t> <t>antibody</t> <t>conjugated</t> with <t>alkaline</t> <t>phosphatase,</t> membranes were developed with NBT/BCIP substrate. Representative membrane is shown. (D) Densitometry of Rrn3 band intensities. Western blot membranes were scanned and Rrn3 and β-actin band densities were measured using ImageJ64 software. Graph shows Rrn3/β-actin ratios adjusted to NPD levels, mean ± SD, n = 6.
    Secondary Antibodies Conjugated To Alkaline Phosphatase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary antibodies conjugated to alkaline phosphatase/product/Bio-Rad
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    secondary antibodies conjugated to alkaline phosphatase - by Bioz Stars, 2020-02
    90/100 stars
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    Image Search Results


    Titrated IgM, IgG1, and IgG2 antibody response in animals vaccinated with salivary gland extract (SGE), midgut extract (ME) or a combination of both SGE and ME (SGE+ME). Plates were coated with 0.5 μg/mL SGE (A,B) or ME (C,D) , respectively.

    Journal: Frontiers in Physiology

    Article Title: Preliminary Evaluation of Tick Protein Extracts and Recombinant Ferritin 2 as Anti-tick Vaccines Targeting Ixodes ricinus in Cattle

    doi: 10.3389/fphys.2018.01696

    Figure Lengend Snippet: Titrated IgM, IgG1, and IgG2 antibody response in animals vaccinated with salivary gland extract (SGE), midgut extract (ME) or a combination of both SGE and ME (SGE+ME). Plates were coated with 0.5 μg/mL SGE (A,B) or ME (C,D) , respectively.

    Article Snippet: The plates were then washed, blocked with 1% fetal bovine serum in PBS, incubated with the sera dilutions followed by specific secondary antibodies against bovine IgG1, IgG2, and IgM conjugated with horseradish peroxidase (Bio-Rad).

    Techniques:

    Adenoviruses expressing capsid-incorporated SP70 epitope elicited SP70-specific humoral immune responses. (A) Immunization regimen showing when immunizations were performed and when sera were collected for antibodies assay and passive protection experiment. (B) Western blot of purified SP70-GST protein (lane 1, 2, 3) and cell extracts from EV71-infected Vero cells (lane 4, 5, 6, 7). The proteins were transferred onto PVDF and reacted with mice antisera raised against R1SP70A3 (lane 1, 4), R2SP70A3 (lane 2, 5), R7SP70A3 (lane 3, 6), or Ad3EGFP (lane 7) respectively. Mice antibodies were detected with an HRP-linked secondary antibody and the signals were developed by ECL. The position of the EV71 VP1 capsid protein is indicated. (C) Total anti-SP70 IgG levels were quantified by indirect ELISA analysis with 1 µg/ml of synthetic SP70 peptide as antigen. Post-prime and post-boost sera were collected at 2, 4, 6, and 8 weeks (wk) for ELISA binding assays. Error bars represent the standard errors of the means per group (n = 5). (D) The distribution of SP70-specific IgG subtypes in the sera. Sera from mice boosted with the antigens were collected at 8-week for ELISA isotype binding assays. ELISA plates were coated with synthetic SP70 peptide. The plates were then incubated with immunized mice sera (anti-EV71-08-02 sera diluted 1∶1000 and other groups antisera diluted 1∶10000). The binding was detected with HRP-conjugated secondary isotype specific antibodies. OD at 450 nm represents isotype-specific SP70 antibody levels in sera. Data are shown as mean ± SD for each group mice (n = 4) for one of two independent experiments. (E) Early humoral responses to SP70 were determined by ELISA with synthesized SP70 peptide at day 7 following the first immunization with hexon-modified Ad vectors. Data are shown as mean ± SD for each group mice (n = 4). (F) Total anti-EGFP IgG levels were quantified by indirect ELISA analysis with 1 µg/ml of recombinant EGFP as antigen. Post-prime and post-boost sera were collected at 2, 4, 6, and 8 weeks (wk) for ELISA binding assays. Data are shown as means ± SD per group (n = 5).

    Journal: PLoS ONE

    Article Title: Protection against Enterovirus 71 with Neutralizing Epitope Incorporation within Adenovirus Type 3 Hexon

    doi: 10.1371/journal.pone.0041381

    Figure Lengend Snippet: Adenoviruses expressing capsid-incorporated SP70 epitope elicited SP70-specific humoral immune responses. (A) Immunization regimen showing when immunizations were performed and when sera were collected for antibodies assay and passive protection experiment. (B) Western blot of purified SP70-GST protein (lane 1, 2, 3) and cell extracts from EV71-infected Vero cells (lane 4, 5, 6, 7). The proteins were transferred onto PVDF and reacted with mice antisera raised against R1SP70A3 (lane 1, 4), R2SP70A3 (lane 2, 5), R7SP70A3 (lane 3, 6), or Ad3EGFP (lane 7) respectively. Mice antibodies were detected with an HRP-linked secondary antibody and the signals were developed by ECL. The position of the EV71 VP1 capsid protein is indicated. (C) Total anti-SP70 IgG levels were quantified by indirect ELISA analysis with 1 µg/ml of synthetic SP70 peptide as antigen. Post-prime and post-boost sera were collected at 2, 4, 6, and 8 weeks (wk) for ELISA binding assays. Error bars represent the standard errors of the means per group (n = 5). (D) The distribution of SP70-specific IgG subtypes in the sera. Sera from mice boosted with the antigens were collected at 8-week for ELISA isotype binding assays. ELISA plates were coated with synthetic SP70 peptide. The plates were then incubated with immunized mice sera (anti-EV71-08-02 sera diluted 1∶1000 and other groups antisera diluted 1∶10000). The binding was detected with HRP-conjugated secondary isotype specific antibodies. OD at 450 nm represents isotype-specific SP70 antibody levels in sera. Data are shown as mean ± SD for each group mice (n = 4) for one of two independent experiments. (E) Early humoral responses to SP70 were determined by ELISA with synthesized SP70 peptide at day 7 following the first immunization with hexon-modified Ad vectors. Data are shown as mean ± SD for each group mice (n = 4). (F) Total anti-EGFP IgG levels were quantified by indirect ELISA analysis with 1 µg/ml of recombinant EGFP as antigen. Post-prime and post-boost sera were collected at 2, 4, 6, and 8 weeks (wk) for ELISA binding assays. Data are shown as means ± SD per group (n = 5).

    Article Snippet: The membranes were blocked with 5% skim-milk in PBS and then incubated with the anti-SP70-GST serum (1∶250), washed and exposed to a 1∶10000 dilution of goat anti-mouse IgG (H + L)-HRP conjugate affinity-purified secondary antibody (Bio-Rad).

    Techniques: Expressing, Western Blot, Purification, Infection, Mouse Assay, Indirect ELISA, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation, Synthesized, Modification, Recombinant

    Confocal and electron microscopy of rat chondrocytes immuno-cytochemically stained with COX-2 and caveolin antibodies. Cells were double stained with fluorescein isothiocyanate- and Texas Red-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under a confocal microscope at their respective wavelengths. For each experiment, a least 50 cells were examined and the presented images represent typical staining pattern for the majority of examined cells. a, Cav-3; b, COX-2; c, merged image of a and b; d and e; stained only with secondary antibodies without the prior treatment of either Cav-3 or COX-2 antibodies ( A ). Cells were double stained with different size of gold particle-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under an electron microscope. Arrow showed structure of caveolae. # and * represented COX-2 (15 nm particles) and Cav-3 (10 nm particles), respectively.

    Journal: Journal of Korean Medical Science

    Article Title: Evidence for Cyclooxygenase-2 Association with Caveolin-3 in Primary Cultured Rat Chondrocytes

    doi: 10.3346/jkms.2006.21.1.100

    Figure Lengend Snippet: Confocal and electron microscopy of rat chondrocytes immuno-cytochemically stained with COX-2 and caveolin antibodies. Cells were double stained with fluorescein isothiocyanate- and Texas Red-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under a confocal microscope at their respective wavelengths. For each experiment, a least 50 cells were examined and the presented images represent typical staining pattern for the majority of examined cells. a, Cav-3; b, COX-2; c, merged image of a and b; d and e; stained only with secondary antibodies without the prior treatment of either Cav-3 or COX-2 antibodies ( A ). Cells were double stained with different size of gold particle-conjugated secondary antibodies after an initial treatment with either Cav-3 or COX-2 antibodies separately and then examined under an electron microscope. Arrow showed structure of caveolae. # and * represented COX-2 (15 nm particles) and Cav-3 (10 nm particles), respectively.

    Article Snippet: After washing, cells were double stained with FITC- and Texas Red-conjugated secondary antibodies for 30 min. After washing, samples were examined at the appropriate wavelengths under a Bio-Rad MRC 1000 confocal microscope (Hercules, CA, U.S.A.).

    Techniques: Electron Microscopy, Staining, Microscopy

    The effect of maternal diet on expression levels of Rrn3 transcription factor in the offspring. (A) A cartoon illustrating the role of Rrn3 in mediating extracellular signals to rDNA transcription by Pol I. (B) RT PCR analysis of Rrn3 transcript levels in 17 dpc and adult kidneys. RNA purified from kidneys was treated with DNase, reverse transcribed with random hexamer primers, and analyzed by qPCR with two different pairs of primers to the last exon of Rrn3 gene. Transcript levels were normalized to two control transcripts, Cypa and Lamc1 , data were combined, adjusted to NPD levels, and presented as mean ± SD. (C) Western blot analysis of Rrn3 protein levels in adult kidneys. Extracts from kidneys (male), 50 μg total protein per lane, were electrophoresed in SDS gels, transferred to PVDF membrane and probed consecutively with antibodies to Rrn3 and β-actin proteins. After incubation with secondary antibody conjugated with alkaline phosphatase, membranes were developed with NBT/BCIP substrate. Representative membrane is shown. (D) Densitometry of Rrn3 band intensities. Western blot membranes were scanned and Rrn3 and β-actin band densities were measured using ImageJ64 software. Graph shows Rrn3/β-actin ratios adjusted to NPD levels, mean ± SD, n = 6.

    Journal: Biochimica et Biophysica Acta

    Article Title: Regulation of ribosomal RNA expression across the lifespan is fine-tuned by maternal diet before implantation

    doi: 10.1016/j.bbagrm.2016.04.001

    Figure Lengend Snippet: The effect of maternal diet on expression levels of Rrn3 transcription factor in the offspring. (A) A cartoon illustrating the role of Rrn3 in mediating extracellular signals to rDNA transcription by Pol I. (B) RT PCR analysis of Rrn3 transcript levels in 17 dpc and adult kidneys. RNA purified from kidneys was treated with DNase, reverse transcribed with random hexamer primers, and analyzed by qPCR with two different pairs of primers to the last exon of Rrn3 gene. Transcript levels were normalized to two control transcripts, Cypa and Lamc1 , data were combined, adjusted to NPD levels, and presented as mean ± SD. (C) Western blot analysis of Rrn3 protein levels in adult kidneys. Extracts from kidneys (male), 50 μg total protein per lane, were electrophoresed in SDS gels, transferred to PVDF membrane and probed consecutively with antibodies to Rrn3 and β-actin proteins. After incubation with secondary antibody conjugated with alkaline phosphatase, membranes were developed with NBT/BCIP substrate. Representative membrane is shown. (D) Densitometry of Rrn3 band intensities. Western blot membranes were scanned and Rrn3 and β-actin band densities were measured using ImageJ64 software. Graph shows Rrn3/β-actin ratios adjusted to NPD levels, mean ± SD, n = 6.

    Article Snippet: After washes, secondary antibodies conjugated to alkaline phosphatase (Bio Rad, Hercules, CA, USA) were added for 1 h at room temperature.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Purification, Random Hexamer Labeling, Real-time Polymerase Chain Reaction, Western Blot, Incubation, Software