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sd leu g418 plates  (Gold Biotechnology Inc)
96
Gold Biotechnology Inc sd leu g418 plates
a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic <t>G418.</t> b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.
Sd Leu G418 Plates, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sd agar plates  (Thermo Fisher)
99
Thermo Fisher sd agar plates
a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic <t>G418.</t> b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.
Sd Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sd dropout plates  (Fisher Scientific)
90
Fisher Scientific sd dropout plates
a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic <t>G418.</t> b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.
Sd Dropout Plates, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sd-trp-leu-his plates  (BIOTEC Co Ltd)
90
BIOTEC Co Ltd sd-trp-leu-his plates
a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic <t>G418.</t> b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.
Sd Trp Leu His Plates, supplied by BIOTEC Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sd-trp-leu-his plates/product/BIOTEC Co Ltd
Average 90 stars, based on 1 article reviews
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sd leu trp plates  (TaKaRa)
95
TaKaRa sd leu trp plates
a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic <t>G418.</t> b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.
Sd Leu Trp Plates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutant library transformant sd trp agar plates  (Teknova)
94
Teknova mutant library transformant sd trp agar plates
a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic <t>G418.</t> b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.
Mutant Library Transformant Sd Trp Agar Plates, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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position 187 site directed mutant library transformant sd trp agar plates  (Teknova)
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Teknova position 187 site directed mutant library transformant sd trp agar plates
a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic <t>G418.</t> b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.
Position 187 Site Directed Mutant Library Transformant Sd Trp Agar Plates, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic G418. b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.

Journal: Nature Communications

Article Title: Generating combinatorial diversity via engineered V(D)J-like recombination in Saccharomyces cerevisiae

doi: 10.1038/s41467-025-61206-1

Figure Lengend Snippet: a Diagram of pY112-CJA-UP-H20 recombination target plasmid. Triangles represent RSSs. Cutting by RAG1/2 leads to homology-assisted DNA repair of the G418R gene that confers resistance to the antibiotic G418. b Yeast strains were engineered with both RAG1 and RAG2 genes (either full-length, core, or core NLS variants) and optionally HMGB1. After 4-d induction in SG-Leu media, cell cultures were plated on Leu, G418 plates and colonies were counted after outgrowth. c G418R recombination with yeast strains assessing the combination of full-length RAG2 with RAG1core. Cells were plated after a 4-d induction. d G418R recombination with alternate truncations of RAG1 that include more regions of the full-length protein than the initial RAG1core construct (which contains amino acids 383-1006). All strains include full-length RAG2 and HMGB1. Cells were plated after a 4-d induction. C = core protein, F = full-length protein, N-C = core protein with N-terminal nuclear localization signal, NLS = nuclear localization signal, CFU = colony forming unit. In ( b – d ) strains that appear in multiple plots are highlighted with a color, and data are presented as mean values ± SD; n = 3 biological replicates. Statistical significance was calculated with a one-way ANOVA and Tukey test (ns = not significant, ** p < 0.01, and **** p < 0.0001). From left to right, the highlighted p values are, in ( b ), p = <0.0001 and 0.0042; in ( c ), p = <0.0001 and <0.0001; and in ( d ), p = <0.0001 and 0.0148. Source data are provided in the Source Data file.

Article Snippet: Therefore, to generate SD-Leu + G418 plates, YNB was replaced with 1.7 g/L of YNB without ammonium sulfate (BD) and 1 g/L of monosodium glutamate (TCI) and supplemented with 300 μg/mL of G418 sulfate (Gold Biotechnology).

Techniques: Plasmid Preparation, Construct