anti sap30  (Bethyl)

Journal: European Journal of Medical Research

Article Title: SAP30 promotes clear cell renal cell carcinoma proliferation and inhibits apoptosis through the MT1G axis

doi: 10.1186/s40001-025-02440-7

Figure Lengend Snippet: SAP30 knockdown promotes the expression of the MT1G and p53 proteins, while MT1G knockdown reverses the alterations in p53 protein levels, cell proliferation and apoptosis-related phenotypes caused by the knockdown of SAP30. A Venn diagram showing shared genes between the downregulated differentially expressed genes in the KIRC TCGA dataset (|log2FC|> 2; *p value < 0.05) and transcription factors in the ENCODE database. B The top 30 enriched GO terms for 251 genes. C Protein expression of p53 and MT1G in the si-Ctrl, si-SAP30, si-MT1G and si-S + M groups. D – F CCK-8 and colony formation assays were used to analyse the effects of si-Ctrl, si-SAP30, si-MT1G and si-S + M on the proliferation of 786-O and CAKI-1 cells. G , H Flow cytometry was used to detect apoptosis in the si-Ctrl, si-SAP30, si-MT1G and si-S + M groups of 786-O and CAKI-1 cells. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The siRNAs for SAP30 and MT1G were purchased from GenePharma (Suzhou, China), and their sequences are shown in Supplementary Table S1.

Techniques: Knockdown, Expressing, CCK-8 Assay, Flow Cytometry

Journal: European Journal of Medical Research

Article Title: SAP30 promotes clear cell renal cell carcinoma proliferation and inhibits apoptosis through the MT1G axis

doi: 10.1186/s40001-025-02440-7

Figure Lengend Snippet: SAP30 binds to the MT1G promoter region to repress MT1G expression. A Immunofluorescence was used to determine the location of SAP30 in 786-O and CAKI-1 cells. B The MT1G mRNA expression data were obtained from TCGA. C Spearman correlation between MT1G methylation and mRNA expression in the KIRC TCGA dataset. D , E mRNA and protein levels of MT1G were determined in the si-Ctrl, si-SAP30 and HDACi groups. F Immunohistochemical staining for the SAP30, MT1G and p53 proteins in tumour tissues. G , H Correlations between the levels of SAP30 and MT1G and p53 were analysed. I Dual-luciferase reporter assay results showing the activity of the MT1G promoter fragment. J ChIP‒PCR analysis of the binding of SAP30 to the MT1G promoter. K Binding site base pair sequences are indicated with boxes. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The siRNAs for SAP30 and MT1G were purchased from GenePharma (Suzhou, China), and their sequences are shown in Supplementary Table S1.

Techniques: Expressing, Immunofluorescence, Methylation, Immunohistochemical staining, Staining, Luciferase, Reporter Assay, Activity Assay, Binding Assay

Journal: European Journal of Medical Research

Article Title: SAP30 promotes clear cell renal cell carcinoma proliferation and inhibits apoptosis through the MT1G axis

doi: 10.1186/s40001-025-02440-7

Figure Lengend Snippet: SAP30 knockdown inhibits ccRCC progression in vivo. A , B The subcutaneous tumours in 12 nude mice and the isolated tumours are shown. C , D The volumes and weights of the tumours were measured in nude mice. E The tumour volumes were measured at 6, 12, 24 and 48 days after tumour formation. F MT1G mRNA expression was measured by qRT‒PCR in the si-Ctrl and si-SAP30 groups in vivo. G The protein expression levels of SAP30, MT1G and p53 were determined in the si-Ctrl and si-SAP30 groups in vivo. H Hypothetical model of the proposed mechanism by which SAP30 regulates the proliferation and apoptosis of ccRCC cells through the MT1G/p53 axis. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The siRNAs for SAP30 and MT1G were purchased from GenePharma (Suzhou, China), and their sequences are shown in Supplementary Table S1.

Techniques: Knockdown, In Vivo, Isolation, Expressing