san hq nuclease  (ArcticZymes)

Journal: Vaccine: X

Article Title: Efficient and scalable clarification of Orf virus from HEK suspension for vaccine development

doi: 10.1016/j.jvacx.2024.100474

Figure Lengend Snippet: Filter capacities and maximum pressure reached for the filtrations using the Sartopure® PP3 filters. Filtration was targeted to be performed until approximately 1 bar, for filtrations with maximum pressure indicated below 1 bar filtration was stopped earlier due to limited available cell culture material. The values were rounded to whole numbers.

Article Snippet: Subsequently, crude harvest was aliquoted in 0.5–1 L flasks and 60 U∙mL −1 of the nucleases M-SAN HQ or Denarase® were added together with magnesium chloride in a final concentration of 10 mM for M-SAN HQ and 2 mM for Denarase®, and incubated for 4 h at 160 rpm in a MaxQ™ 6000 shaking incubator (Thermo Fisher Scientific) at 37 °C.

Techniques: Filtration, Cell Culture, Membrane, Pore Size, Negative Control