sagescience bluepippin system  (Sage Science)

 
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    Name:
    BluePippin
    Description:
    DNA Size Selection System The BluePippin has the DNA capabilities of the Pippin Prep plus the extra benefit of pulsed field electrophoresis for resolving and collecting high molecular weight DNA For long range genomic applications high pass filtering allows users to collect all fragments above a size threshold set by the user Target sizes or ranges of sizes are entered in software and fractions are collected in buffer Up to 5 samples gel cassette may be run with no possibility of cross contamination
    Catalog Number:
    blu0001
    Price:
    None
    Quantity:
    ea
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    Structured Review

    Sage Science sagescience bluepippin system
    BluePippin
    DNA Size Selection System The BluePippin has the DNA capabilities of the Pippin Prep plus the extra benefit of pulsed field electrophoresis for resolving and collecting high molecular weight DNA For long range genomic applications high pass filtering allows users to collect all fragments above a size threshold set by the user Target sizes or ranges of sizes are entered in software and fractions are collected in buffer Up to 5 samples gel cassette may be run with no possibility of cross contamination
    https://www.bioz.com/result/sagescience bluepippin system/product/Sage Science
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sagescience bluepippin system - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Marker:

    Article Title: Robust Benchmark Structural Variant Calls of An Asian Using the State-of-Art Long Fragment Sequencing Technologies
    Article Snippet: .. 5 ug genomic DNA was sheared to ∼15 kb fragments by a g-TUBE (#520079, Covaris) centrifugation (2,000 Xg, 2 min, twice), was size-selected for 10 kb using the BluePippin system (Sage Science, MA) by marker (0.75% DF Marker S1 High-Pass 6-10kb vs3) for the 10-20 kb DNA target fragments. .. Quality control of the libraries was performed by Qubit fluorometer (Life Technologies, CA) and Bioanalyzer 2100 (Agilent, CA).

    Article Title: Effect of Short-Term Antiretroviral Therapy Interruption on Levels of Integrated HIV DNA
    Article Snippet: .. For each sample, 10 μg of DNA was loaded into a 0.75% agarose gel cassette with an external S1 marker and size selected using a 20-kb high-pass protocol on a BluePippin pulsed-field gel electrophoresis system (Sage Science) to remove unintegrated viral DNA species. .. HIV DNA levels were quantified by qPCR with primer and probe targeting HIV LTR/ gag and normalized to cellular input by qPCR targeting the CCR5 gene ( ).

    Centrifugation:

    Article Title: Robust Benchmark Structural Variant Calls of An Asian Using the State-of-Art Long Fragment Sequencing Technologies
    Article Snippet: .. 5 ug genomic DNA was sheared to ∼15 kb fragments by a g-TUBE (#520079, Covaris) centrifugation (2,000 Xg, 2 min, twice), was size-selected for 10 kb using the BluePippin system (Sage Science, MA) by marker (0.75% DF Marker S1 High-Pass 6-10kb vs3) for the 10-20 kb DNA target fragments. .. Quality control of the libraries was performed by Qubit fluorometer (Life Technologies, CA) and Bioanalyzer 2100 (Agilent, CA).

    Selection:

    Article Title: An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes
    Article Snippet: .. The concentrated sample containing a pool of 96 libraries was subjected to size selection on a BluePippin (Sage Science, Beverly, USA). ..

    Article Title: The genetic architecture underlying ecological adaptation in Atlantic herring is not consistent with the infinitesimal model
    Article Snippet: .. Size selection for molecules over 40 kb using BluePippin (SageScience), and libraries for PacBio sequencing was made using the manufacturer’s protocol. .. Sequencing was carried out on one PacBio Sequel II 8M SMRT Cell for 15 h to generate 125 Gb CLR sequence data.

    Article Title: Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications
    Article Snippet: .. DNA integrity can be estimated using pulsed-field gel electrophoresis and the adequate fraction selected for downstream sequencing using the Blue pippin DNA size selection system (BluePippin; Sage Science, Beverly, MA, USA). .. RNA extraction protocol For RNA extraction, the biological material was kept frozen at all times until the addition of sorbitol buffer.

    Agarose Gel Electrophoresis:

    Article Title: Effect of Short-Term Antiretroviral Therapy Interruption on Levels of Integrated HIV DNA
    Article Snippet: .. For each sample, 10 μg of DNA was loaded into a 0.75% agarose gel cassette with an external S1 marker and size selected using a 20-kb high-pass protocol on a BluePippin pulsed-field gel electrophoresis system (Sage Science) to remove unintegrated viral DNA species. .. HIV DNA levels were quantified by qPCR with primer and probe targeting HIV LTR/ gag and normalized to cellular input by qPCR targeting the CCR5 gene ( ).

    Sequencing:

    Article Title: The genetic architecture underlying ecological adaptation in Atlantic herring is not consistent with the infinitesimal model
    Article Snippet: .. Size selection for molecules over 40 kb using BluePippin (SageScience), and libraries for PacBio sequencing was made using the manufacturer’s protocol. .. Sequencing was carried out on one PacBio Sequel II 8M SMRT Cell for 15 h to generate 125 Gb CLR sequence data.

    Article Title: Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications
    Article Snippet: .. DNA integrity can be estimated using pulsed-field gel electrophoresis and the adequate fraction selected for downstream sequencing using the Blue pippin DNA size selection system (BluePippin; Sage Science, Beverly, MA, USA). .. RNA extraction protocol For RNA extraction, the biological material was kept frozen at all times until the addition of sorbitol buffer.

    Amplification:

    Article Title: ddRAD-seq derived genome-wide SNPs, high density linkage map and QTLs for fruit quality traits in strawberry (Fragaria x ananassa)
    Article Snippet: .. The adaptor-ligated DNA fragments were amplified by 22 cycles of polymerase chain reaction and the amplicons of 300–900 bp length were separated using BluePippin (Sage Science, MA, USA). .. The extracted DNA fragments were then sequenced by the Illumina HiSeq 2000 platform (Illumina, Inc., CA, USA) using 93 base pair (bp) paired-end (PE) mode following the procedures described in Shirasawa et al. ( ).

    Polymerase Chain Reaction:

    Article Title: ddRAD-seq derived genome-wide SNPs, high density linkage map and QTLs for fruit quality traits in strawberry (Fragaria x ananassa)
    Article Snippet: .. The adaptor-ligated DNA fragments were amplified by 22 cycles of polymerase chain reaction and the amplicons of 300–900 bp length were separated using BluePippin (Sage Science, MA, USA). .. The extracted DNA fragments were then sequenced by the Illumina HiSeq 2000 platform (Illumina, Inc., CA, USA) using 93 base pair (bp) paired-end (PE) mode following the procedures described in Shirasawa et al. ( ).

    Pulsed-Field Gel:

    Article Title: Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications
    Article Snippet: .. DNA integrity can be estimated using pulsed-field gel electrophoresis and the adequate fraction selected for downstream sequencing using the Blue pippin DNA size selection system (BluePippin; Sage Science, Beverly, MA, USA). .. RNA extraction protocol For RNA extraction, the biological material was kept frozen at all times until the addition of sorbitol buffer.

    Article Title: Effect of Short-Term Antiretroviral Therapy Interruption on Levels of Integrated HIV DNA
    Article Snippet: .. For each sample, 10 μg of DNA was loaded into a 0.75% agarose gel cassette with an external S1 marker and size selected using a 20-kb high-pass protocol on a BluePippin pulsed-field gel electrophoresis system (Sage Science) to remove unintegrated viral DNA species. .. HIV DNA levels were quantified by qPCR with primer and probe targeting HIV LTR/ gag and normalized to cellular input by qPCR targeting the CCR5 gene ( ).

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  • 99
    Sage Science sagescience bluepippin system
    Sagescience Bluepippin System, supplied by Sage Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sagescience bluepippin system/product/Sage Science
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sagescience bluepippin system - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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